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The map label for this gene is amiC [H]

Identifier: 158423382

GI number: 158423382

Start: 2009211

End: 2010515

Strand: Direct

Name: amiC [H]

Synonym: AZC_1758

Alternate gene names: 158423382

Gene position: 2009211-2010515 (Clockwise)

Preceding gene: 158423379

Following gene: 158423383

Centisome position: 37.42

GC content: 62.61

Gene sequence:

>1305_bases
ATGCTCGGTTTGCTGGGAAGAGCGGCTAAGGGACTTGGCGCGATGGCGGTAGCCGCCGCGGTTGCCGGTTCCATGGGGAC
GGCCGCCGCGCAGGAGACGATCAAGGTCGGCGTGCTTCACTCGCTGTCCGGCACCATGGCGATCAGCGAGACCACGCTGA
AGGACGCCGTGCTCATGATGATCGAAGAGCAGAACAAGAAGGGCGGCGTGCTCGGCAAGAAGCTCGAGGCGGTGGTGGTG
GACCCGGCGTCCAACTGGCCGCTGTTCGCCGAGAAGGCCCGCGAACTCATCACCAAGGACAAGGTGTCCGTGGTGTTCGG
CTGCTGGACCTCGGTGAGCCGCAAGTCCGTGCTGCCGGTGTTCAAGGAGCTGAACTCCATCCTCTTCTACCCCGTTCAGT
ATGAGGGCGAGGAGAGCGAGCGGAACGTGTTCTACACCGGCGCCGCGCCGAACCAGCAGGCGATCCCGGCGGTGGATTAC
CTCGCCAAGGAAGAGAAGGTGGAGCGCTGGGTGCTTGCGGGCACCGACTATGTCTATCCCCGCACCACCAACAAGATCCT
TGAGGCCTATCTGAAGTCCCGCGGCGTGAAGGCCGAGGACATCATGATCAACTACACGCCCTTCGGTCACTCCGACTGGC
AGACCATCGTCGCCGACATCAAGAAGTTCGGCTCGGCCGGCAAGAAGACCGCTGTGGTCTCCACCATCAACGGCGACGCC
AACGTGCCTTTCTACAAGGAACTGGCGAACCAGGGCATCAAGGCCACCGACATCCCGGTCGTGGCCTTCTCGGTGGGCGA
AGAAGAGCTCGCCGGCATCGACACCAAGCCGCTCGTCGGCCATCTCGCTGCCTGGAACTACTTCGAGTCCATCAAGACCC
CGGAGAACGACGCCTTCATCAAGGCCTGGCAGACCTTCACCAAGAACCCGAAGCGCGTGACCAACGACCCCATGGAAGCC
ACCTATATCGGCTTCAACATGTGGGTTAAGGCCGTGGAGAAGGCCGGCACGGTCGATCCGGACAAGGTGATCGCCACCCT
GCCGGGCACCAAGCAGCCGAACCTCACCGGCGGCGTCTCGGAGATGCTCCCGAACCACCACATCACCAAGCCCGTCTTCA
TCGGCGAGATCCAGGGCGACGGCCAGTTCAACGTGGTGTGGAAGACGGACGATCTGGTGCCCGGCGAAGCCTGGTCCAAG
TATCTCGAAGGCTCCAAGAACCTCGTTGCCGACTGGGTGAAGTACAACTGCGGCAACTACGACACCACCAAGAACACCTG
CCTCGGCGCCACCGCCGCCAAGTGA

Upstream 100 bases:

>100_bases
GTATACAATCATTCGGTGCCCTTCGCGGACATGGCACGCTGCTTGCTGATCTCCCTGCCGCTGGCACAGCGCCAGCCCAT
TTTCATCGGGGAGAGAGCGG

Downstream 100 bases:

>100_bases
GCAGCAATGTGAGCTGAGGCGGCTCGGTCCTTGGGAAAAGCCCTCTCCCGCTTGCGGGGGAGGGAACCTGTCCGGAGCGG
GGAGGGCGCACTCTTGTCTC

Product: urea short-chain amide uptake protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MLGLLGRAAKGLGAMAVAAAVAGSMGTAAAQETIKVGVLHSLSGTMAISETTLKDAVLMMIEEQNKKGGVLGKKLEAVVV
DPASNWPLFAEKARELITKDKVSVVFGCWTSVSRKSVLPVFKELNSILFYPVQYEGEESERNVFYTGAAPNQQAIPAVDY
LAKEEKVERWVLAGTDYVYPRTTNKILEAYLKSRGVKAEDIMINYTPFGHSDWQTIVADIKKFGSAGKKTAVVSTINGDA
NVPFYKELANQGIKATDIPVVAFSVGEEELAGIDTKPLVGHLAAWNYFESIKTPENDAFIKAWQTFTKNPKRVTNDPMEA
TYIGFNMWVKAVEKAGTVDPDKVIATLPGTKQPNLTGGVSEMLPNHHITKPVFIGEIQGDGQFNVVWKTDDLVPGEAWSK
YLEGSKNLVADWVKYNCGNYDTTKNTCLGATAAK

Sequences:

>Translated_434_residues
MLGLLGRAAKGLGAMAVAAAVAGSMGTAAAQETIKVGVLHSLSGTMAISETTLKDAVLMMIEEQNKKGGVLGKKLEAVVV
DPASNWPLFAEKARELITKDKVSVVFGCWTSVSRKSVLPVFKELNSILFYPVQYEGEESERNVFYTGAAPNQQAIPAVDY
LAKEEKVERWVLAGTDYVYPRTTNKILEAYLKSRGVKAEDIMINYTPFGHSDWQTIVADIKKFGSAGKKTAVVSTINGDA
NVPFYKELANQGIKATDIPVVAFSVGEEELAGIDTKPLVGHLAAWNYFESIKTPENDAFIKAWQTFTKNPKRVTNDPMEA
TYIGFNMWVKAVEKAGTVDPDKVIATLPGTKQPNLTGGVSEMLPNHHITKPVFIGEIQGDGQFNVVWKTDDLVPGEAWSK
YLEGSKNLVADWVKYNCGNYDTTKNTCLGATAAK
>Mature_434_residues
MLGLLGRAAKGLGAMAVAAAVAGSMGTAAAQETIKVGVLHSLSGTMAISETTLKDAVLMMIEEQNKKGGVLGKKLEAVVV
DPASNWPLFAEKARELITKDKVSVVFGCWTSVSRKSVLPVFKELNSILFYPVQYEGEESERNVFYTGAAPNQQAIPAVDY
LAKEEKVERWVLAGTDYVYPRTTNKILEAYLKSRGVKAEDIMINYTPFGHSDWQTIVADIKKFGSAGKKTAVVSTINGDA
NVPFYKELANQGIKATDIPVVAFSVGEEELAGIDTKPLVGHLAAWNYFESIKTPENDAFIKAWQTFTKNPKRVTNDPMEA
TYIGFNMWVKAVEKAGTVDPDKVIATLPGTKQPNLTGGVSEMLPNHHITKPVFIGEIQGDGQFNVVWKTDDLVPGEAWSK
YLEGSKNLVADWVKYNCGNYDTTKNTCLGATAAK

Specific function: Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 47184; Mature: 47184

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLLGRAAKGLGAMAVAAAVAGSMGTAAAQETIKVGVLHSLSGTMAISETTLKDAVLMM
CCCCHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHEEEEEECCCCCEEEEHHHHHHHHEEE
IEEQNKKGGVLGKKLEAVVVDPASNWPLFAEKARELITKDKVSVVFGCWTSVSRKSVLPV
EECCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEHHHCCHHHHHHH
FKELNSILFYPVQYEGEESERNVFYTGAAPNQQAIPAVDYLAKEEKVERWVLAGTDYVYP
HHHCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHEEEEECCCEECC
RTTNKILEAYLKSRGVKAEDIMINYTPFGHSDWQTIVADIKKFGSAGKKTAVVSTINGDA
CCHHHHHHHHHHHCCCEEHHEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCC
NVPFYKELANQGIKATDIPVVAFSVGEEELAGIDTKPLVGHLAAWNYFESIKTPENDAFI
CCCHHHHHHHCCCCCCCCCEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHH
KAWQTFTKNPKRVTNDPMEATYIGFNMWVKAVEKAGTVDPDKVIATLPGTKQPNLTGGVS
HHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCCCCHH
EMLPNHHITKPVFIGEIQGDGQFNVVWKTDDLVPGEAWSKYLEGSKNLVADWVKYNCGNY
HHCCCCCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCC
DTTKNTCLGATAAK
CCCCCCCCCCCCCC
>Mature Secondary Structure
MLGLLGRAAKGLGAMAVAAAVAGSMGTAAAQETIKVGVLHSLSGTMAISETTLKDAVLMM
CCCCHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHEEEEEECCCCCEEEEHHHHHHHHEEE
IEEQNKKGGVLGKKLEAVVVDPASNWPLFAEKARELITKDKVSVVFGCWTSVSRKSVLPV
EECCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEHHHCCHHHHHHH
FKELNSILFYPVQYEGEESERNVFYTGAAPNQQAIPAVDYLAKEEKVERWVLAGTDYVYP
HHHCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHEEEEECCCEECC
RTTNKILEAYLKSRGVKAEDIMINYTPFGHSDWQTIVADIKKFGSAGKKTAVVSTINGDA
CCHHHHHHHHHHHCCCEEHHEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCC
NVPFYKELANQGIKATDIPVVAFSVGEEELAGIDTKPLVGHLAAWNYFESIKTPENDAFI
CCCHHHHHHHCCCCCCCCCEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHH
KAWQTFTKNPKRVTNDPMEATYIGFNMWVKAVEKAGTVDPDKVIATLPGTKQPNLTGGVS
HHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCCCCHH
EMLPNHHITKPVFIGEIQGDGQFNVVWKTDDLVPGEAWSKYLEGSKNLVADWVKYNCGNY
HHCCCCCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCC
DTTKNTCLGATAAK
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907262; 10984043; 1762155; 7813419; 10508151; 10708652 [H]