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The map label for this gene is pdhC [H]

Identifier: 158423368

GI number: 158423368

Start: 1994570

End: 1995949

Strand: Direct

Name: pdhC [H]

Synonym: AZC_1744

Alternate gene names: 158423368

Gene position: 1994570-1995949 (Clockwise)

Preceding gene: 158423367

Following gene: 158423369

Centisome position: 37.14

GC content: 70.65

Gene sequence:

>1380_bases
ATGCCCATCGAGATCCTGATGCCGGCCCTGTCTCCCACCATGGAGAAGGGCAACCTCGCCAAGTGGCTCAAGAAGGAAGG
TGACAGCGTCAAGTCCGGCGACGTCATCGCCGAGATCGAGACCGACAAGGCCACCATGGAAGTGGAGGCCGTCGACGAGG
GCGTCCTCGCCAAGATCGTGGTGCCGGAAGGGTCGCAGGACGTGCCGGTGAACCAGCTCATCGCGGTTCTGGCCGGCGAG
GGCGAGGATGTCGCCGCGGCTGCGGCCTCCGCCGGCTCCGGCGGCGCCAAGCCGGCCGCGGCGCCCGCTCCGGCGGCGGC
CCCGGCTGCGGCTCCCGCCGCTGCCCCGGCGCCTGTGGCGGCTCCGGCTGCGGCCCCCGCTCCGGCGGCCGCGCCTGTCG
CTGCCGCCCCCACCGGTGGCCGCGTGTTCGCCTCGCCGCTGGCCCGTCGCCTCGCGAAGGAGAAGGGCATCGATCTCGCG
GCGCTGGCGGGCTCCGGTCCGCGCGGCCGCATCATCGCCCGCGACGTGGAAGGGGCGAAGCCCGGTGCGGCTCCGGCTGC
CGCCGCTGCGGCCCCGGCCGCTGCCGCGCCCGCTCCCGCTCCCAAGGCGACTGCGGCTGCCGCGCCCGCCGTCTCCGGCC
CGACCGCCGATCAGGTCAAGGCGCTGTTCGCCGAGGGCACCTATGAGGAGGTGCAGCTCGATGGCATGCGCAAGACCATC
GCCAAGCGCCTTGTGGAGAGCGAGCAGCTCACGCCCACCTTCTACCTCTCGGTGGATTGCGACATCGACGACCTGATGAA
GCTGCGCGAGACGGTCAACGCCTCCGCGCCGAAGGACAAGGACGGCAAGCCCGGCTTCCGCGTCTCGGTCAACGACTTCG
TCATCAAGGCGCTGGCGCTGGCGCTCCAGAAGGTGCCCGCCGCCAATGCCGTGTGGGCCGAGGACCGTATCCTGCGCCTC
AAGCACTCGGACGTGGGCGTCGCGGTGGCCATCGACGGTGGCCTGTTCGCGCCCATCGTGAAGAAGGCGGAGCAGAAGAC
GCTCTCGGCCATCTCCAACGAGATGCGCGACCTCGCCACCCGCGCCCGCACCAAGAAGCTGAAGCCCGACGAGTATCAGG
GCGGCTCCACCTCGGTGTCGAACCTCGGCATGATGGGCGTGCGCGACTTCGTGGCCATCATCAACGCGCCGCAGTCCTCG
ATCCTGGCGGTCGGCGCCAGCGAGCAGCGGCCGGTGGTGCGTGGCGGCGAGATCAAGATCGCCACCCAGTTCACCGCCAC
CATCACCTGCGATCACCGCGTGATGGATGGTGCGCTGGGCGCCGAATTGCTCGCCGCGTTCAAGGGCTTCATCGAGAACC
CCATGTCCATGCTGGTGTGA

Upstream 100 bases:

>100_bases
GCCGTATGCCGCCAATCTCGAAAAGCTCGCGCTGCCGAACGTCGCCGAGGTGATCGAGGCGGTGCGCGCGGTCACCTATC
GCTGAGCGGGAGGAGCAGAC

Downstream 100 bases:

>100_bases
CCGTCTGGCCGGGGGCGCCCCGCGCCCCCGGTTCCGCGCGGCCCGGTTCTGTCCCTGCGGCAGATCAAATCGCGGGGCGG
CGGGACAGTGTAGCTGCTGG

Product: dihydrolipoamide S-acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MPIEILMPALSPTMEKGNLAKWLKKEGDSVKSGDVIAEIETDKATMEVEAVDEGVLAKIVVPEGSQDVPVNQLIAVLAGE
GEDVAAAAASAGSGGAKPAAAPAPAAAPAAAPAAAPAPVAAPAAAPAPAAAPVAAAPTGGRVFASPLARRLAKEKGIDLA
ALAGSGPRGRIIARDVEGAKPGAAPAAAAAAPAAAAPAPAPKATAAAAPAVSGPTADQVKALFAEGTYEEVQLDGMRKTI
AKRLVESEQLTPTFYLSVDCDIDDLMKLRETVNASAPKDKDGKPGFRVSVNDFVIKALALALQKVPAANAVWAEDRILRL
KHSDVGVAVAIDGGLFAPIVKKAEQKTLSAISNEMRDLATRARTKKLKPDEYQGGSTSVSNLGMMGVRDFVAIINAPQSS
ILAVGASEQRPVVRGGEIKIATQFTATITCDHRVMDGALGAELLAAFKGFIENPMSMLV

Sequences:

>Translated_459_residues
MPIEILMPALSPTMEKGNLAKWLKKEGDSVKSGDVIAEIETDKATMEVEAVDEGVLAKIVVPEGSQDVPVNQLIAVLAGE
GEDVAAAAASAGSGGAKPAAAPAPAAAPAAAPAAAPAPVAAPAAAPAPAAAPVAAAPTGGRVFASPLARRLAKEKGIDLA
ALAGSGPRGRIIARDVEGAKPGAAPAAAAAAPAAAAPAPAPKATAAAAPAVSGPTADQVKALFAEGTYEEVQLDGMRKTI
AKRLVESEQLTPTFYLSVDCDIDDLMKLRETVNASAPKDKDGKPGFRVSVNDFVIKALALALQKVPAANAVWAEDRILRL
KHSDVGVAVAIDGGLFAPIVKKAEQKTLSAISNEMRDLATRARTKKLKPDEYQGGSTSVSNLGMMGVRDFVAIINAPQSS
ILAVGASEQRPVVRGGEIKIATQFTATITCDHRVMDGALGAELLAAFKGFIENPMSMLV
>Mature_458_residues
PIEILMPALSPTMEKGNLAKWLKKEGDSVKSGDVIAEIETDKATMEVEAVDEGVLAKIVVPEGSQDVPVNQLIAVLAGEG
EDVAAAAASAGSGGAKPAAAPAPAAAPAAAPAAAPAPVAAPAAAPAPAAAPVAAAPTGGRVFASPLARRLAKEKGIDLAA
LAGSGPRGRIIARDVEGAKPGAAPAAAAAAPAAAAPAPAPKATAAAAPAVSGPTADQVKALFAEGTYEEVQLDGMRKTIA
KRLVESEQLTPTFYLSVDCDIDDLMKLRETVNASAPKDKDGKPGFRVSVNDFVIKALALALQKVPAANAVWAEDRILRLK
HSDVGVAVAIDGGLFAPIVKKAEQKTLSAISNEMRDLATRARTKKLKPDEYQGGSTSVSNLGMMGVRDFVAIINAPQSSI
LAVGASEQRPVVRGGEIKIATQFTATITCDHRVMDGALGAELLAAFKGFIENPMSMLV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=464, Percent_Identity=40.948275862069, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI203098816, Length=484, Percent_Identity=35.7438016528926, Blast_Score=271, Evalue=9e-73,
Organism=Homo sapiens, GI203098753, Length=484, Percent_Identity=35.7438016528926, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=40.9937888198758, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI110671329, Length=443, Percent_Identity=25.7336343115124, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI19923748, Length=230, Percent_Identity=30, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786305, Length=435, Percent_Identity=32.6436781609195, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1786946, Length=459, Percent_Identity=27.8867102396514, Blast_Score=148, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17560088, Length=463, Percent_Identity=44.4924406047516, Blast_Score=339, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI17538894, Length=316, Percent_Identity=32.9113924050633, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=457, Percent_Identity=27.7899343544858, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17537937, Length=444, Percent_Identity=25.4504504504505, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324258, Length=467, Percent_Identity=43.254817987152, Blast_Score=315, Evalue=7e-87,
Organism=Saccharomyces cerevisiae, GI6320352, Length=456, Percent_Identity=28.5087719298246, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321632, Length=177, Percent_Identity=36.1581920903955, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI20129315, Length=467, Percent_Identity=44.3254817987152, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24582497, Length=456, Percent_Identity=43.859649122807, Blast_Score=271, Evalue=5e-73,
Organism=Drosophila melanogaster, GI18859875, Length=450, Percent_Identity=27.1111111111111, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46634; Mature: 46503

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIEILMPALSPTMEKGNLAKWLKKEGDSVKSGDVIAEIETDKATMEVEAVDEGVLAKIV
CCCEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
VPEGSQDVPVNQLIAVLAGEGEDVAAAAASAGSGGAKPAAAPAPAAAPAAAPAAAPAPVA
ECCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APAAAPAPAAAPVAAAPTGGRVFASPLARRLAKEKGIDLAALAGSGPRGRIIARDVEGAK
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCCCC
PGAAPAAAAAAPAAAAPAPAPKATAAAAPAVSGPTADQVKALFAEGTYEEVQLDGMRKTI
CCCCCHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
AKRLVESEQLTPTFYLSVDCDIDDLMKLRETVNASAPKDKDGKPGFRVSVNDFVIKALAL
HHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHH
ALQKVPAANAVWAEDRILRLKHSDVGVAVAIDGGLFAPIVKKAEQKTLSAISNEMRDLAT
HHHHCCCCCCEECCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RARTKKLKPDEYQGGSTSVSNLGMMGVRDFVAIINAPQSSILAVGASEQRPVVRGGEIKI
HHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCHHEEEECCCCCCCCEECCEEEE
ATQFTATITCDHRVMDGALGAELLAAFKGFIENPMSMLV
EEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCHHHHCC
>Mature Secondary Structure 
PIEILMPALSPTMEKGNLAKWLKKEGDSVKSGDVIAEIETDKATMEVEAVDEGVLAKIV
CCEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
VPEGSQDVPVNQLIAVLAGEGEDVAAAAASAGSGGAKPAAAPAPAAAPAAAPAAAPAPVA
ECCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APAAAPAPAAAPVAAAPTGGRVFASPLARRLAKEKGIDLAALAGSGPRGRIIARDVEGAK
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCCCC
PGAAPAAAAAAPAAAAPAPAPKATAAAAPAVSGPTADQVKALFAEGTYEEVQLDGMRKTI
CCCCCHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
AKRLVESEQLTPTFYLSVDCDIDDLMKLRETVNASAPKDKDGKPGFRVSVNDFVIKALAL
HHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHH
ALQKVPAANAVWAEDRILRLKHSDVGVAVAIDGGLFAPIVKKAEQKTLSAISNEMRDLAT
HHHHCCCCCCEECCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RARTKKLKPDEYQGGSTSVSNLGMMGVRDFVAIINAPQSSILAVGASEQRPVVRGGEIKI
HHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCHHEEEECCCCCCCCEECCEEEE
ATQFTATITCDHRVMDGALGAELLAAFKGFIENPMSMLV
EEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]