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The map label for this gene is pdhB [H]

Identifier: 158423367

GI number: 158423367

Start: 1993154

End: 1994554

Strand: Direct

Name: pdhB [H]

Synonym: AZC_1743

Alternate gene names: 158423367

Gene position: 1993154-1994554 (Clockwise)

Preceding gene: 158423366

Following gene: 158423368

Centisome position: 37.12

GC content: 67.02

Gene sequence:

>1401_bases
ATGCCCGTCGATATTCTCATGCCGGCCCTGTCTCCGACCATGGAGAAGGGCAACCTCACGAAGTGGGTGAAGAAGGAAGG
CGACACGGTCAAGGCCGGCGACGTCATCGCCGAGATCGAGACCGACAAGGCCACCATGGAAGTGGAGGCCGTGGACGAGG
GCATCCTCGGCAAGATCCTGATCCCGGAAGGCACGCAGGACGTGGCCGTGAACACCCCCATCGCCGTGATCCTTGGCGAG
GGTGAGGACGCCTCCGCAGCCAGCACGCCGGCGCCGCAGCAGAAGGTCGCCGAGAGCGCCGCCCCGGCCTCCCCGGTGGC
CGCCGCCGCCCCGGCGCCGCAGGCGAGCGTGCCCTCCGCCGTCGCCAACCCGCCGGTCGTCACCTCCCAGCCTGATCCGG
AAGTGCCCGCGGGCACCGAGATGGTCACGATGACCGTGCGCGAAGCGCTGCGCGACGCCATGGCCGAAGAGATGCGCCGC
GACGGCGACGTCTTCGTGATGGGTGAGGAGGTCGCCGAATATCAGGGCGCCTACAAGATCACCCAGGGCCTCCTGCAGGA
ATTCGGCGCCAAGCGCGTGGTGGACACCCCCATCACCGAACACGGCTTCGCCGGCATGGGCGTGGGCGCCGCCATGGCGG
GCCTCAAGCCCATCATCGAGTTCATGACCTTCAACTTCGCCATGCAGGCGATCGACCAGATCATCAACTCCGCCGCCAAG
ACGCTCTACATGTCCGGCGGCCAGGTGCAGTGCTCGGTGGTGTTCCGCGGCCCGAACGGCGCCGCCGCCCGCGTCGCCGC
CCAGCACAGCCAGGATTATGCGGCCTGGTACAGCCACATCCCCGGCCTCAAGGTGGTGGCGCCTTATACGGCGGCAGACG
CCAAGGGCCTGCTCAAGGCGGCCATCCGCGATCCGAACCCGGTCATCTTCCTTGAGAACGAGATCCTCTACGGCCACTCC
TTCGAGGTGCCGAAGCTGGACGATTACGTGCTGCCCATCGGCAAGGCCCGCATCGCCCGCGCCGGCAAGGATGTGACGCT
CGTCTCCTGGTCCATCGGCATGACCTATACGCTGAAGGCGGCCGAGGAACTGGCCAAGCAGGGCATCGAGGCGGAGGTCA
TCGACCTGCGCACGATCCGTCCCATGGACGTGCCCACCATCATCGAATCGGTGAAGAAGACCGGCCGCTGCGTCACCGTC
GAGGAAGGCTGGCCGCAGTCCGGCGTCGGTTCCGAGATCGCCGCCCAGCTCATGGAAAAGGCGTTCGACTATCTCGACGC
TCCCGTGCTGCGCGTGACCGGCAAGGATGTGCCCATGCCGTATGCCGCCAATCTCGAAAAGCTCGCGCTGCCGAACGTCG
CCGAGGTGATCGAGGCGGTGCGCGCGGTCACCTATCGCTGA

Upstream 100 bases:

>100_bases
TTGCCGCGGTCTCCGTTTACACCAACCTTTCGGCCGTGCGCCCGCCGTCTGCCGCTCCGGCAGCCCCGGGAGCCGCTCCG
TCCAGCAAGAGCTGACAGCC

Downstream 100 bases:

>100_bases
GCGGGAGGAGCAGACATGCCCATCGAGATCCTGATGCCGGCCCTGTCTCCCACCATGGAGAAGGGCAACCTCGCCAAGTG
GCTCAAGAAGGAAGGTGACA

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MPVDILMPALSPTMEKGNLTKWVKKEGDTVKAGDVIAEIETDKATMEVEAVDEGILGKILIPEGTQDVAVNTPIAVILGE
GEDASAASTPAPQQKVAESAAPASPVAAAAPAPQASVPSAVANPPVVTSQPDPEVPAGTEMVTMTVREALRDAMAEEMRR
DGDVFVMGEEVAEYQGAYKITQGLLQEFGAKRVVDTPITEHGFAGMGVGAAMAGLKPIIEFMTFNFAMQAIDQIINSAAK
TLYMSGGQVQCSVVFRGPNGAAARVAAQHSQDYAAWYSHIPGLKVVAPYTAADAKGLLKAAIRDPNPVIFLENEILYGHS
FEVPKLDDYVLPIGKARIARAGKDVTLVSWSIGMTYTLKAAEELAKQGIEAEVIDLRTIRPMDVPTIIESVKKTGRCVTV
EEGWPQSGVGSEIAAQLMEKAFDYLDAPVLRVTGKDVPMPYAANLEKLALPNVAEVIEAVRAVTYR

Sequences:

>Translated_466_residues
MPVDILMPALSPTMEKGNLTKWVKKEGDTVKAGDVIAEIETDKATMEVEAVDEGILGKILIPEGTQDVAVNTPIAVILGE
GEDASAASTPAPQQKVAESAAPASPVAAAAPAPQASVPSAVANPPVVTSQPDPEVPAGTEMVTMTVREALRDAMAEEMRR
DGDVFVMGEEVAEYQGAYKITQGLLQEFGAKRVVDTPITEHGFAGMGVGAAMAGLKPIIEFMTFNFAMQAIDQIINSAAK
TLYMSGGQVQCSVVFRGPNGAAARVAAQHSQDYAAWYSHIPGLKVVAPYTAADAKGLLKAAIRDPNPVIFLENEILYGHS
FEVPKLDDYVLPIGKARIARAGKDVTLVSWSIGMTYTLKAAEELAKQGIEAEVIDLRTIRPMDVPTIIESVKKTGRCVTV
EEGWPQSGVGSEIAAQLMEKAFDYLDAPVLRVTGKDVPMPYAANLEKLALPNVAEVIEAVRAVTYR
>Mature_465_residues
PVDILMPALSPTMEKGNLTKWVKKEGDTVKAGDVIAEIETDKATMEVEAVDEGILGKILIPEGTQDVAVNTPIAVILGEG
EDASAASTPAPQQKVAESAAPASPVAAAAPAPQASVPSAVANPPVVTSQPDPEVPAGTEMVTMTVREALRDAMAEEMRRD
GDVFVMGEEVAEYQGAYKITQGLLQEFGAKRVVDTPITEHGFAGMGVGAAMAGLKPIIEFMTFNFAMQAIDQIINSAAKT
LYMSGGQVQCSVVFRGPNGAAARVAAQHSQDYAAWYSHIPGLKVVAPYTAADAKGLLKAAIRDPNPVIFLENEILYGHSF
EVPKLDDYVLPIGKARIARAGKDVTLVSWSIGMTYTLKAAEELAKQGIEAEVIDLRTIRPMDVPTIIESVKKTGRCVTVE
EGWPQSGVGSEIAAQLMEKAFDYLDAPVLRVTGKDVPMPYAANLEKLALPNVAEVIEAVRAVTYR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=60.1851851851852, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=57.0987654320988, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI4557353, Length=365, Percent_Identity=36.7123287671233, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI34101272, Length=365, Percent_Identity=36.7123287671233, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI203098753, Length=89, Percent_Identity=51.685393258427, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI203098816, Length=89, Percent_Identity=51.685393258427, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI31711992, Length=84, Percent_Identity=51.1904761904762, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI260898739, Length=59, Percent_Identity=62.7118644067797, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI225637459, Length=243, Percent_Identity=30.8641975308642, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI225637461, Length=243, Percent_Identity=30.8641975308642, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI225637463, Length=243, Percent_Identity=31.2757201646091, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI133778974, Length=154, Percent_Identity=33.1168831168831, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17538422, Length=323, Percent_Identity=66.5634674922601, Blast_Score=438, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17506935, Length=346, Percent_Identity=40.4624277456647, Blast_Score=220, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17560088, Length=142, Percent_Identity=40.1408450704225, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17539652, Length=261, Percent_Identity=31.0344827586207, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319698, Length=326, Percent_Identity=61.3496932515337, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6324258, Length=129, Percent_Identity=44.9612403100775, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321632, Length=119, Percent_Identity=42.8571428571429, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21358145, Length=324, Percent_Identity=62.3456790123457, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24650940, Length=324, Percent_Identity=62.3456790123457, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI160714832, Length=323, Percent_Identity=39.0092879256966, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI160714828, Length=323, Percent_Identity=39.0092879256966, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=64.4444444444444, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=64.4444444444444, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI20129315, Length=72, Percent_Identity=55.5555555555556, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI45551847, Length=254, Percent_Identity=27.1653543307087, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45550715, Length=254, Percent_Identity=27.1653543307087, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24645119, Length=254, Percent_Identity=27.1653543307087, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24582497, Length=65, Percent_Identity=52.3076923076923, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 49499; Mature: 49368

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVDILMPALSPTMEKGNLTKWVKKEGDTVKAGDVIAEIETDKATMEVEAVDEGILGKIL
CCHHHCCCCCCCCCCCCCCHHHHHCCCCEEECCCEEEEEECCCCEEEEEECCCCCEEEEE
IPEGTQDVAVNTPIAVILGEGEDASAASTPAPQQKVAESAAPASPVAAAAPAPQASVPSA
CCCCCCCEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHH
VANPPVVTSQPDPEVPAGTEMVTMTVREALRDAMAEEMRRDGDVFVMGEEVAEYQGAYKI
HCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHH
TQGLLQEFGAKRVVDTPITEHGFAGMGVGAAMAGLKPIIEFMTFNFAMQAIDQIINSAAK
HHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLYMSGGQVQCSVVFRGPNGAAARVAAQHSQDYAAWYSHIPGLKVVAPYTAADAKGLLKA
EEEECCCEEEEEEEEECCCCCHHHHHHHHCCHHHHHHHCCCCCEEEECCCHHHHHHHHHH
AIRDPNPVIFLENEILYGHSFEVPKLDDYVLPIGKARIARAGKDVTLVSWSIGMTYTLKA
HHCCCCCEEEEECEEEECCCCCCCCCCCEECCCCHHHHHHCCCCEEEEEEECCCEEEHHH
AEELAKQGIEAEVIDLRTIRPMDVPTIIESVKKTGRCVTVEEGWPQSGVGSEIAAQLMEK
HHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHH
AFDYLDAPVLRVTGKDVPMPYAANLEKLALPNVAEVIEAVRAVTYR
HHHHHCCCEEEECCCCCCCCHHCCCHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVDILMPALSPTMEKGNLTKWVKKEGDTVKAGDVIAEIETDKATMEVEAVDEGILGKIL
CHHHCCCCCCCCCCCCCCHHHHHCCCCEEECCCEEEEEECCCCEEEEEECCCCCEEEEE
IPEGTQDVAVNTPIAVILGEGEDASAASTPAPQQKVAESAAPASPVAAAAPAPQASVPSA
CCCCCCCEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHH
VANPPVVTSQPDPEVPAGTEMVTMTVREALRDAMAEEMRRDGDVFVMGEEVAEYQGAYKI
HCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHH
TQGLLQEFGAKRVVDTPITEHGFAGMGVGAAMAGLKPIIEFMTFNFAMQAIDQIINSAAK
HHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLYMSGGQVQCSVVFRGPNGAAARVAAQHSQDYAAWYSHIPGLKVVAPYTAADAKGLLKA
EEEECCCEEEEEEEEECCCCCHHHHHHHHCCHHHHHHHCCCCCEEEECCCHHHHHHHHHH
AIRDPNPVIFLENEILYGHSFEVPKLDDYVLPIGKARIARAGKDVTLVSWSIGMTYTLKA
HHCCCCCEEEEECEEEECCCCCCCCCCCEECCCCHHHHHHCCCCEEEEEEECCCEEEHHH
AEELAKQGIEAEVIDLRTIRPMDVPTIIESVKKTGRCVTVEEGWPQSGVGSEIAAQLMEK
HHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHH
AFDYLDAPVLRVTGKDVPMPYAANLEKLALPNVAEVIEAVRAVTYR
HHHHHCCCEEEECCCCCCCCHHCCCHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]