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The map label for this gene is pcm [H]

Identifier: 158423352

GI number: 158423352

Start: 1977779

End: 1978480

Strand: Direct

Name: pcm [H]

Synonym: AZC_1728

Alternate gene names: 158423352

Gene position: 1977779-1978480 (Clockwise)

Preceding gene: 158423351

Following gene: 158423353

Centisome position: 36.83

GC content: 69.94

Gene sequence:

>702_bases
ATGGGGCGCGAAGGTGCTTTACAGTCGCATCGTCAGGGGGCGCTTGGCATGACCGACAGTGAAGCCGAACGGGCGGAGCG
GGCCGCCTTCATCCTCAGCCTGCGGCGGCGGGGCATCCGGGATCTGGCGGTGCTGCGGGCGCTGGAGCTGGTGCCGCGCG
GCCTCTTCGTGGACCCGACTTTGCGTCGCCATGCCTATGAGGATGTGGCACTCCCCATCGCCTGCGGGCAGACCATGTCC
CAGCCGAGCCTCGTCGCCCTGATGACCGAGGCACTCGCGCTCAATGCCGAGCATACGGTGCTCGAGATTGGCACGGGGTC
GGGCTATCAGGCCGCCGTCCTCTCCCATCTCGCGGCGCAGGTGGTGACCATGGACCGCTACCGGGCGCTGGTGGGCGAGG
CGCAGACGCGCTTTCAGGTGCTCGGCCTGCGCAACGTCGCCGCCTTCGTGGGGGACGGCACGCAGGGCCTGCCGGGCCGC
GCGCCCTATGATCGCATCATGATCACCGCCGCAACCGGGGAGGTGCCCCGCGCGCTGGTGGATCAACTGAAACCGGGCGG
CGTCCTCATCGCCCCCATCGGCGCGCCCCGCGAGGTGCAGAAGCTCCGCCGGTTCATCAAGGATGGCGGCAACCTTGAGG
CGAGCGACCTCATGGACGTGCGCTTCGTGCCGCTGGTCGCCGGGGTGGCCGCGCTGCTCTGA

Upstream 100 bases:

>100_bases
ACGCCAACCATGTGCATCTCGACATGCGCCAGCGCCGCAACGATTTCCGCATGTGCCAGTGGAATGTGGACTAGGCGGGC
GCCCGCGCCCCCGTGTTTCA

Downstream 100 bases:

>100_bases
TCGGTTCCGCCGACGCCGCGCGGACCCCTCCGATCTTCCCAAGGCCGACGAAGTCGCGCCGGTTCGCTCCGCCCGTGATG
GGCGGTGGGGGCTGGCGTGT

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MGREGALQSHRQGALGMTDSEAERAERAAFILSLRRRGIRDLAVLRALELVPRGLFVDPTLRRHAYEDVALPIACGQTMS
QPSLVALMTEALALNAEHTVLEIGTGSGYQAAVLSHLAAQVVTMDRYRALVGEAQTRFQVLGLRNVAAFVGDGTQGLPGR
APYDRIMITAATGEVPRALVDQLKPGGVLIAPIGAPREVQKLRRFIKDGGNLEASDLMDVRFVPLVAGVAALL

Sequences:

>Translated_233_residues
MGREGALQSHRQGALGMTDSEAERAERAAFILSLRRRGIRDLAVLRALELVPRGLFVDPTLRRHAYEDVALPIACGQTMS
QPSLVALMTEALALNAEHTVLEIGTGSGYQAAVLSHLAAQVVTMDRYRALVGEAQTRFQVLGLRNVAAFVGDGTQGLPGR
APYDRIMITAATGEVPRALVDQLKPGGVLIAPIGAPREVQKLRRFIKDGGNLEASDLMDVRFVPLVAGVAALL
>Mature_232_residues
GREGALQSHRQGALGMTDSEAERAERAAFILSLRRRGIRDLAVLRALELVPRGLFVDPTLRRHAYEDVALPIACGQTMSQ
PSLVALMTEALALNAEHTVLEIGTGSGYQAAVLSHLAAQVVTMDRYRALVGEAQTRFQVLGLRNVAAFVGDGTQGLPGRA
PYDRIMITAATGEVPRALVDQLKPGGVLIAPIGAPREVQKLRRFIKDGGNLEASDLMDVRFVPLVAGVAALL

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=213, Percent_Identity=31.4553990610329, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1789100, Length=200, Percent_Identity=48, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI71983477, Length=191, Percent_Identity=30.8900523560209, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI193207222, Length=189, Percent_Identity=29.1005291005291, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI17981723, Length=219, Percent_Identity=30.1369863013699, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 24940; Mature: 24809

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGREGALQSHRQGALGMTDSEAERAERAAFILSLRRRGIRDLAVLRALELVPRGLFVDPT
CCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEECCH
LRRHAYEDVALPIACGQTMSQPSLVALMTEALALNAEHTVLEIGTGSGYQAAVLSHLAAQ
HHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHH
VVTMDRYRALVGEAQTRFQVLGLRNVAAFVGDGTQGLPGRAPYDRIMITAATGEVPRALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHH
DQLKPGGVLIAPIGAPREVQKLRRFIKDGGNLEASDLMDVRFVPLVAGVAALL
HCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
GREGALQSHRQGALGMTDSEAERAERAAFILSLRRRGIRDLAVLRALELVPRGLFVDPT
CCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEECCH
LRRHAYEDVALPIACGQTMSQPSLVALMTEALALNAEHTVLEIGTGSGYQAAVLSHLAAQ
HHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHH
VVTMDRYRALVGEAQTRFQVLGLRNVAAFVGDGTQGLPGRAPYDRIMITAATGEVPRALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHH
DQLKPGGVLIAPIGAPREVQKLRRFIKDGGNLEASDLMDVRFVPLVAGVAALL
HCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA