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The map label for this gene is gph [C]

Identifier: 158423110

GI number: 158423110

Start: 1686875

End: 1687582

Strand: Reverse

Name: gph [C]

Synonym: AZC_1486

Alternate gene names: 158423110

Gene position: 1687582-1686875 (Counterclockwise)

Preceding gene: 158423112

Following gene: 158423109

Centisome position: 31.43

GC content: 69.77

Gene sequence:

>708_bases
ATGACCGGCCTGAAGGCGCTCCTGTTCGACAAGGACGGCACCCTCGTGCATTTCGACCACACTTGGGGACCGGCCGCCGG
CGCGGTGATGCGGGCGCTCGCGGGGGACGATCCCCACGCACTGAAGCGCCTTGAGGAGGTGAGCCACTATCTGCCCGAGG
AGAGCCGCTTCCTGCCCTCCTCGCCGCTGGTGGCGGGCTCCTCCGCCCATTACGGGCCGCAGTGGGCGCAGTGCCTTGGC
CGGCAGGCGGACACCGCCTTCCTGACCGAAATCGACAGGCTCTTCTCCGACGAGGGCCTGAAAGCGCTGACGCCGCTCGG
CCGGCCGGCCGACGTGCTCGGGCATTTTCACCGGCAGGGGATGCGGCTCGGCATCGTGACCAACGATGCGGAAGCCAATT
CCCGCCTTCAGGCCGAGGCGCTGGGGCTTACGCCTTTCCTCGATCTCATCCACGGTTATGATTCCGGCTTCGGCTCCAAG
CCCGGTCCCGGCATGGTAAGCGCCGCCGCCGAACGGCTGGGCCTTGCCCCGCACCAGACGGCGGTGATCGGCGACAGCGC
CCACGACATGGCCGCCGCCCACAGCGCCGGCGCCCGTTTCATCCTCGTGCGCACGGGCCCCGCGCCGGTGGAGCATCTCT
ATGATGCCGCCGATCTCGTGGTGGACAGCATCGACGACCTGCCGGCGCGGCTTGCGGCGGCCGCCTGA

Upstream 100 bases:

>100_bases
GAGGGTGTTTTTCCGTTCAATCATTGCGAACAACATAGTAAATGACGCCCTCTCCGGCGTCACCGCCCCTCCCTTACCGC
TCCACGGAACGAAGCTCCCC

Downstream 100 bases:

>100_bases
GGGGGCGGGCTTCCGCTTCCGCGCGCGCTGGCCTAGACAGAATGGGAGGCGGGCACCTGAAGCCCGCAGGCAGGGTCCGG
CATGATCAAGCGACGGCTCT

Product: phosphatase

Products: glycolate; phosphate

Alternate protein names: Phosphoglycolate Phosphatase; Phosphatase; Haloacid Dehalogenase-Like Hydrolase; HAD Superfamily Hydrolase; Haloacid Dehalogenase Domain Protein Hydrolase; HAD-Like Hydrolase; Hydrolase; Phosphoglycolate Phosphatase Protein

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MTGLKALLFDKDGTLVHFDHTWGPAAGAVMRALAGDDPHALKRLEEVSHYLPEESRFLPSSPLVAGSSAHYGPQWAQCLG
RQADTAFLTEIDRLFSDEGLKALTPLGRPADVLGHFHRQGMRLGIVTNDAEANSRLQAEALGLTPFLDLIHGYDSGFGSK
PGPGMVSAAAERLGLAPHQTAVIGDSAHDMAAAHSAGARFILVRTGPAPVEHLYDAADLVVDSIDDLPARLAAAA

Sequences:

>Translated_235_residues
MTGLKALLFDKDGTLVHFDHTWGPAAGAVMRALAGDDPHALKRLEEVSHYLPEESRFLPSSPLVAGSSAHYGPQWAQCLG
RQADTAFLTEIDRLFSDEGLKALTPLGRPADVLGHFHRQGMRLGIVTNDAEANSRLQAEALGLTPFLDLIHGYDSGFGSK
PGPGMVSAAAERLGLAPHQTAVIGDSAHDMAAAHSAGARFILVRTGPAPVEHLYDAADLVVDSIDDLPARLAAAA
>Mature_234_residues
TGLKALLFDKDGTLVHFDHTWGPAAGAVMRALAGDDPHALKRLEEVSHYLPEESRFLPSSPLVAGSSAHYGPQWAQCLGR
QADTAFLTEIDRLFSDEGLKALTPLGRPADVLGHFHRQGMRLGIVTNDAEANSRLQAEALGLTPFLDLIHGYDSGFGSKP
GPGMVSAAAERLGLAPHQTAVIGDSAHDMAAAHSAGARFILVRTGPAPVEHLYDAADLVVDSIDDLPARLAAAA

Specific function: Unknown

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789787, Length=119, Percent_Identity=33.6134453781513, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.3.18

Molecular weight: Translated: 24811; Mature: 24680

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLKALLFDKDGTLVHFDHTWGPAAGAVMRALAGDDPHALKRLEEVSHYLPEESRFLPS
CCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
SPLVAGSSAHYGPQWAQCLGRQADTAFLTEIDRLFSDEGLKALTPLGRPADVLGHFHRQG
CCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHCC
MRLGIVTNDAEANSRLQAEALGLTPFLDLIHGYDSGFGSKPGPGMVSAAAERLGLAPHQT
CEEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCE
AVIGDSAHDMAAAHSAGARFILVRTGPAPVEHLYDAADLVVDSIDDLPARLAAAA
EEECCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGLKALLFDKDGTLVHFDHTWGPAAGAVMRALAGDDPHALKRLEEVSHYLPEESRFLPS
CCCEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
SPLVAGSSAHYGPQWAQCLGRQADTAFLTEIDRLFSDEGLKALTPLGRPADVLGHFHRQG
CCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHCC
MRLGIVTNDAEANSRLQAEALGLTPFLDLIHGYDSGFGSKPGPGMVSAAAERLGLAPHQT
CEEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCE
AVIGDSAHDMAAAHSAGARFILVRTGPAPVEHLYDAADLVVDSIDDLPARLAAAA
EEECCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.57 {phosphoglycolate}} [C]

Substrates: 2-phosphoglycolate; H2O

Specific reaction: 2-phosphoglycolate + H2O = glycolate + phosphate

General reaction: Additional information:

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA