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The map label for this gene is xthA [H]

Identifier: 158423021

GI number: 158423021

Start: 1589230

End: 1590042

Strand: Reverse

Name: xthA [H]

Synonym: AZC_1397

Alternate gene names: 158423021

Gene position: 1590042-1589230 (Counterclockwise)

Preceding gene: 158423022

Following gene: 158423020

Centisome position: 29.61

GC content: 68.76

Gene sequence:

>813_bases
ATGCGGATCGCCACTTTCAACGTGAACGGCGTCAATGGACGCCTGCCGCCTCTGCTGGACTGGCTCCGGCAGAGCAAGCC
CGACGTGGCCTGCCTCCAGGAGCTGAAGGCCCCGCACGAGCGCTTTCCCGCCGCCGAACTGGAACAGGCGGGCTATGGCG
CGGTCTGGCTAGGGCAGAAGGCCTGGAACGGGGTTGCCATTCTCGCCCGCGGCGAGATGCCGGTCGAGACCCGCCGCGGT
CTTCCCGGCGATCCGGACGATATCCAGAGCCGCTATATCGAGGCCGCCGTCGGCGGCGTCCTGATCGGCTGCCTCTATGC
GCCGAACGGCAATCCGGCACCGGGCGCGAAATTCGACTACAAGCTGCGCTGGCTTGAGCGGCTGCGAAATTACAGCGCGC
AGATGCTCGACCTCGACCTGCCCATGGTGCTCGCAGGCGATTTCAACATCATGCCCGAGGAGCGCGACGTCTATGCGCCG
GAGCGCTGGCGGGACGACGCGCTGTTCCGCCCGGAAGTGCGCGCCGCGTTCGCCGATTTGCGGGACGATGGATGGGTGGA
CGCGCTGCGCCACCTCCACCCCGACGCGCGCCTCTACACCTTCTGGAAATACCTGCGAAACGCCTTTGCCCGCGACGCCG
GGCTGCGGATCGACCATCTGCTGCTCAGCCCGGCGCTCGCCCCGCGCCTCGTGTCGGCCGGCGTGGATCGTGCCGTGCGC
GGGCGCGACCATGCCAGCGATCACGCACCAGCCTGGATCACGCTTGATGCGCCGACACGGGGACACGCGGGCGGCGATCG
CCGGACGGCCTGA

Upstream 100 bases:

>100_bases
GTTTCTAGCCCTCGCGTCTTCTGGGGGCCGCCAGCGACCGGATCGAACCGGACCGGGGCCTTGAGCGTTGATGGGCTTCC
TGCCTCACGCCGGGGCCGCC

Downstream 100 bases:

>100_bases
CGAGTCCCGATCCATAGCGAGCTAGGCACAGCAGGCGCGACGGTATCATGTGGAAGATCAATAGCCATTGATGAACGGCC
AGCCCGACAAATTTTGGGCG

Product: exodeoxyribonuclease III protein

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MRIATFNVNGVNGRLPPLLDWLRQSKPDVACLQELKAPHERFPAAELEQAGYGAVWLGQKAWNGVAILARGEMPVETRRG
LPGDPDDIQSRYIEAAVGGVLIGCLYAPNGNPAPGAKFDYKLRWLERLRNYSAQMLDLDLPMVLAGDFNIMPEERDVYAP
ERWRDDALFRPEVRAAFADLRDDGWVDALRHLHPDARLYTFWKYLRNAFARDAGLRIDHLLLSPALAPRLVSAGVDRAVR
GRDHASDHAPAWITLDAPTRGHAGGDRRTA

Sequences:

>Translated_270_residues
MRIATFNVNGVNGRLPPLLDWLRQSKPDVACLQELKAPHERFPAAELEQAGYGAVWLGQKAWNGVAILARGEMPVETRRG
LPGDPDDIQSRYIEAAVGGVLIGCLYAPNGNPAPGAKFDYKLRWLERLRNYSAQMLDLDLPMVLAGDFNIMPEERDVYAP
ERWRDDALFRPEVRAAFADLRDDGWVDALRHLHPDARLYTFWKYLRNAFARDAGLRIDHLLLSPALAPRLVSAGVDRAVR
GRDHASDHAPAWITLDAPTRGHAGGDRRTA
>Mature_270_residues
MRIATFNVNGVNGRLPPLLDWLRQSKPDVACLQELKAPHERFPAAELEQAGYGAVWLGQKAWNGVAILARGEMPVETRRG
LPGDPDDIQSRYIEAAVGGVLIGCLYAPNGNPAPGAKFDYKLRWLERLRNYSAQMLDLDLPMVLAGDFNIMPEERDVYAP
ERWRDDALFRPEVRAAFADLRDDGWVDALRHLHPDARLYTFWKYLRNAFARDAGLRIDHLLLSPALAPRLVSAGVDRAVR
GRDHASDHAPAWITLDAPTRGHAGGDRRTA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=262, Percent_Identity=31.2977099236641, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI18375503, Length=262, Percent_Identity=31.2977099236641, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI18375501, Length=262, Percent_Identity=31.2977099236641, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=35.9550561797753, Blast_Score=146, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71989536, Length=237, Percent_Identity=29.1139240506329, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221330655, Length=266, Percent_Identity=31.203007518797, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17136678, Length=266, Percent_Identity=31.203007518797, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30189; Mature: 30189

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATFNVNGVNGRLPPLLDWLRQSKPDVACLQELKAPHERFPAAELEQAGYGAVWLGQK
CEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHCCCHHHCCHHHHHHCCCCEEEECCC
AWNGVAILARGEMPVETRRGLPGDPDDIQSRYIEAAVGGVLIGCLYAPNGNPAPGAKFDY
CCCCEEEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHH
KLRWLERLRNYSAQMLDLDLPMVLAGDFNIMPEERDVYAPERWRDDALFRPEVRAAFADL
HHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHH
RDDGWVDALRHLHPDARLYTFWKYLRNAFARDAGLRIDHLLLSPALAPRLVSAGVDRAVR
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GRDHASDHAPAWITLDAPTRGHAGGDRRTA
CCCCCCCCCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MRIATFNVNGVNGRLPPLLDWLRQSKPDVACLQELKAPHERFPAAELEQAGYGAVWLGQK
CEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHCCCHHHCCHHHHHHCCCCEEEECCC
AWNGVAILARGEMPVETRRGLPGDPDDIQSRYIEAAVGGVLIGCLYAPNGNPAPGAKFDY
CCCCEEEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHH
KLRWLERLRNYSAQMLDLDLPMVLAGDFNIMPEERDVYAPERWRDDALFRPEVRAAFADL
HHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHH
RDDGWVDALRHLHPDARLYTFWKYLRNAFARDAGLRIDHLLLSPALAPRLVSAGVDRAVR
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GRDHASDHAPAWITLDAPTRGHAGGDRRTA
CCCCCCCCCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]