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The map label for this gene is cysD [H]

Identifier: 158318129

GI number: 158318129

Start: 7761018

End: 7761977

Strand: Reverse

Name: cysD [H]

Synonym: Franean1_6393

Alternate gene names: 158318129

Gene position: 7761977-7761018 (Counterclockwise)

Preceding gene: 158318130

Following gene: 158318128

Centisome position: 86.42

GC content: 66.15

Gene sequence:

>960_bases
ATGACGCCGGTCGACGGTACGGACACACCGGTACGGACGCAGAAGGGTAGCCCTGGCATGGGCCGACTGACACATCTGCA
GCGCCTGGAGGCGGAGAGCATCCAGATCTTCCGGGAGGCGGTGGCGGAGAGCGAACGGCCGGTGATGCTGTATTCGGTGG
GCAAGGACAGCACCGTGCTGTTGCACCTGGCGATGAAGGCGTTCTTCCCGTCGCGGCCGCCGTTCCCGCTGCTGCATGTG
GACACGACGTGGAAGTTCCGGGCGATGTACGACTTCCGGGACAAGGCCGCCGCGGAGCTCGGTGTCGACCTGCTCGTCTA
TCAGAACCCGGAGTGCGTGGAACGGGGCATCAACCCGTTCGACCATGGTTCGGCGACGCACACGGACATGTGGAAGACCG
AGGGACTGAAGCAGGCGCTGGACCTGTACGGGTTCGACCTGGCGTTCGGTGGTGCGCGCCGCGACGAGGAGAAGTCGCGG
GCGAAGGAGCGGGTGTTCTCGATCCGCTCGGCGCAGCACCGGTGGGACCCGAAGGCACAGCGTCCGGAGCTGTGGCGGTT
GTACAACGCGAGCAAGGCACCGGGGCAGAGCGTGCGGGTGTTCCCGCTGTCGAACTGGACCGAGCTGGACGTGTGGCAGT
ACATCCACCGGGAGAAGATCCCGATCGTGCCGTTGTACTACGCGGCGCCGCGCCCGGTCGTGGAACGCGACGGCGCTCTG
ATCATGGTGGATGACGACCGGATGCGGCTGGAGCCGGGGGAGGTGCCCGAGCAGCGCAGTGTCCGGTTCCGGACGTTGGG
CTGCTACCCGCTGACCGGCGCGGTGGAAAGCACCGCCGGAACCCTCCCGGAGATCATCCAGGAGATGTTGCTGACGACGA
GCTCGGAACGCCAGGGCCGGGTGATCGACCATGACTCGTCCGGGTCGATGGAGAAGAAGAAGCAGGAGGGGTACTTCTGA

Upstream 100 bases:

>100_bases
CCCCGGCCGTAGCGGGCAACGGCACTGCGGGCGGGAGCACTGCGGGCGGCGGCATCACCGGCGGCATCACCGGCGGCAAC
GACGAGGAAAGGCCCCGGTC

Downstream 100 bases:

>100_bases
TGGCGCACGCGGCCAGCGACCTCGTCGCGGACGACATCGAGGCGTACCTGAACCTGCACGAGCACAAGAGCATGCTGCGG
TTCATCACCTGCGGCAGTGT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTPVDGTDTPVRTQKGSPGMGRLTHLQRLEAESIQIFREAVAESERPVMLYSVGKDSTVLLHLAMKAFFPSRPPFPLLHV
DTTWKFRAMYDFRDKAAAELGVDLLVYQNPECVERGINPFDHGSATHTDMWKTEGLKQALDLYGFDLAFGGARRDEEKSR
AKERVFSIRSAQHRWDPKAQRPELWRLYNASKAPGQSVRVFPLSNWTELDVWQYIHREKIPIVPLYYAAPRPVVERDGAL
IMVDDDRMRLEPGEVPEQRSVRFRTLGCYPLTGAVESTAGTLPEIIQEMLLTTSSERQGRVIDHDSSGSMEKKKQEGYF

Sequences:

>Translated_319_residues
MTPVDGTDTPVRTQKGSPGMGRLTHLQRLEAESIQIFREAVAESERPVMLYSVGKDSTVLLHLAMKAFFPSRPPFPLLHV
DTTWKFRAMYDFRDKAAAELGVDLLVYQNPECVERGINPFDHGSATHTDMWKTEGLKQALDLYGFDLAFGGARRDEEKSR
AKERVFSIRSAQHRWDPKAQRPELWRLYNASKAPGQSVRVFPLSNWTELDVWQYIHREKIPIVPLYYAAPRPVVERDGAL
IMVDDDRMRLEPGEVPEQRSVRFRTLGCYPLTGAVESTAGTLPEIIQEMLLTTSSERQGRVIDHDSSGSMEKKKQEGYF
>Mature_318_residues
TPVDGTDTPVRTQKGSPGMGRLTHLQRLEAESIQIFREAVAESERPVMLYSVGKDSTVLLHLAMKAFFPSRPPFPLLHVD
TTWKFRAMYDFRDKAAAELGVDLLVYQNPECVERGINPFDHGSATHTDMWKTEGLKQALDLYGFDLAFGGARRDEEKSRA
KERVFSIRSAQHRWDPKAQRPELWRLYNASKAPGQSVRVFPLSNWTELDVWQYIHREKIPIVPLYYAAPRPVVERDGALI
MVDDDRMRLEPGEVPEQRSVRFRTLGCYPLTGAVESTAGTLPEIIQEMLLTTSSERQGRVIDHDSSGSMEKKKQEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=298, Percent_Identity=70.4697986577181, Blast_Score=443, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 36369; Mature: 36238

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPVDGTDTPVRTQKGSPGMGRLTHLQRLEAESIQIFREAVAESERPVMLYSVGKDSTVL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEH
LHLAMKAFFPSRPPFPLLHVDTTWKFRAMYDFRDKAAAELGVDLLVYQNPECVERGINPF
HHHHHHHHCCCCCCCCEEEECCCEEEEEEECHHHHHHHHHCCEEEEECCHHHHHHCCCCC
DHGSATHTDMWKTEGLKQALDLYGFDLAFGGARRDEEKSRAKERVFSIRSAQHRWDPKAQ
CCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
RPELWRLYNASKAPGQSVRVFPLSNWTELDVWQYIHREKIPIVPLYYAAPRPVVERDGAL
CCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCEE
IMVDDDRMRLEPGEVPEQRSVRFRTLGCYPLTGAVESTAGTLPEIIQEMLLTTSSERQGR
EEEECCCEECCCCCCCCCCCCEEEEECCEECCCCHHHHCCCHHHHHHHHHHCCCCCCCCC
VIDHDSSGSMEKKKQEGYF
EEECCCCCCHHHHHHCCCC
>Mature Secondary Structure 
TPVDGTDTPVRTQKGSPGMGRLTHLQRLEAESIQIFREAVAESERPVMLYSVGKDSTVL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEH
LHLAMKAFFPSRPPFPLLHVDTTWKFRAMYDFRDKAAAELGVDLLVYQNPECVERGINPF
HHHHHHHHCCCCCCCCEEEECCCEEEEEEECHHHHHHHHHCCEEEEECCHHHHHHCCCCC
DHGSATHTDMWKTEGLKQALDLYGFDLAFGGARRDEEKSRAKERVFSIRSAQHRWDPKAQ
CCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
RPELWRLYNASKAPGQSVRVFPLSNWTELDVWQYIHREKIPIVPLYYAAPRPVVERDGAL
CCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCEE
IMVDDDRMRLEPGEVPEQRSVRFRTLGCYPLTGAVESTAGTLPEIIQEMLLTTSSERQGR
EEEECCCEECCCCCCCCCCCCEEEEECCEECCCCHHHHCCCHHHHHHHHHHCCCCCCCCC
VIDHDSSGSMEKKKQEGYF
EEECCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA