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The map label for this gene is thiE [H]

Identifier: 158313678

GI number: 158313678

Start: 2211142

End: 2211831

Strand: Direct

Name: thiE [H]

Synonym: Franean1_1842

Alternate gene names: 158313678

Gene position: 2211142-2211831 (Clockwise)

Preceding gene: 158313677

Following gene: 158313679

Centisome position: 24.62

GC content: 76.52

Gene sequence:

>690_bases
GTGCCGTCGCCGGGGGCTGAGCCGCTGCGCGCGCGTCGGCTGGCCCGCCTGCTTGACGCCCGCCTCTACCTGTGCACCGC
GCGTCGCGGCGACGAGGGAAGCTTCCTCGAGGACGTGCTCGGCCTGCCGGGCGCGCCCGCCGTCGACCTCGTCCAGCTCC
GGGAGAAGGGGCTGGAGTGGCGCGAGGAGCTCGCCGGGCTGGCCCGGATGCGGGAGGCCGCGGACCGGGTCGGTGCCCTG
GTCAGCGCCAACGACCGGGCCGACGTCGCAGCCTTCGCGGGCGTCGACATCCTGCATGTGGGGCAGGACGACATCCCGCC
GCGGCTGGCGCGGACCATCGTCGGGGCGGACGCGCTCATCGGGCTGTCCACGCATGATCCCGAGCAGTTCCTGGCCGCGC
TCGCGGACCCGGACGTCGACTACGTGTGCGTCGGCCCGGTGCACGCCACGCCGACCAAGGAGGGCCGTCCACCGGTCGGG
ATGGGCCTGCCGCGGCTCGCCGCGCGGCACGCCCCGCCGTTCGAGCCGGGGGCCAAACCCTGGTTCGTCACCGGCGGTGT
GGACGCGCGGACCCTGGACGCCATCCTCGAGACCGGCGCGCGCCGGGTCGTGGTGGTGCGGGGCATCGCCGAGGCCGCGG
ATCCGGGAGCCGCCGCCACCGCGCTCGCCGAACGCCTGCGCTCGGCCTGA

Upstream 100 bases:

>100_bases
CGGCCCGGCTCGGTGGTCGTGGAACTGCGCGGCACCGCGCTCACCCCGTCGGAGATGCCTTCGGTCCACCTGGCCGACGG
CGACACCCTGGAGATCGTCC

Downstream 100 bases:

>100_bases
CCGGGGGAGGGCGTCCGCGGTGAGCCGCCTCGGTCACTTCGGCGGTCTGGGCCACTTCAGCTACCTCGCGGTGCTGCTGG
GGTGCCTGGTGGCGACGGCG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MPSPGAEPLRARRLARLLDARLYLCTARRGDEGSFLEDVLGLPGAPAVDLVQLREKGLEWREELAGLARMREAADRVGAL
VSANDRADVAAFAGVDILHVGQDDIPPRLARTIVGADALIGLSTHDPEQFLAALADPDVDYVCVGPVHATPTKEGRPPVG
MGLPRLAARHAPPFEPGAKPWFVTGGVDARTLDAILETGARRVVVVRGIAEAADPGAAATALAERLRSA

Sequences:

>Translated_229_residues
MPSPGAEPLRARRLARLLDARLYLCTARRGDEGSFLEDVLGLPGAPAVDLVQLREKGLEWREELAGLARMREAADRVGAL
VSANDRADVAAFAGVDILHVGQDDIPPRLARTIVGADALIGLSTHDPEQFLAALADPDVDYVCVGPVHATPTKEGRPPVG
MGLPRLAARHAPPFEPGAKPWFVTGGVDARTLDAILETGARRVVVVRGIAEAADPGAAATALAERLRSA
>Mature_228_residues
PSPGAEPLRARRLARLLDARLYLCTARRGDEGSFLEDVLGLPGAPAVDLVQLREKGLEWREELAGLARMREAADRVGALV
SANDRADVAAFAGVDILHVGQDDIPPRLARTIVGADALIGLSTHDPEQFLAALADPDVDYVCVGPVHATPTKEGRPPVGM
GLPRLAARHAPPFEPGAKPWFVTGGVDARTLDAILETGARRVVVVRGIAEAADPGAAATALAERLRSA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=191, Percent_Identity=30.8900523560209, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 24118; Mature: 23987

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPGAEPLRARRLARLLDARLYLCTARRGDEGSFLEDVLGLPGAPAVDLVQLREKGLEW
CCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCHHH
REELAGLARMREAADRVGALVSANDRADVAAFAGVDILHVGQDDIPPRLARTIVGADALI
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHEE
GLSTHDPEQFLAALADPDVDYVCVGPVHATPTKEGRPPVGMGLPRLAARHAPPFEPGAKP
ECCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
WFVTGGVDARTLDAILETGARRVVVVRGIAEAADPGAAATALAERLRSA
EEEECCCCHHHHHHHHHCCCCEEEEEECHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PSPGAEPLRARRLARLLDARLYLCTARRGDEGSFLEDVLGLPGAPAVDLVQLREKGLEW
CCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCHHH
REELAGLARMREAADRVGALVSANDRADVAAFAGVDILHVGQDDIPPRLARTIVGADALI
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHEE
GLSTHDPEQFLAALADPDVDYVCVGPVHATPTKEGRPPVGMGLPRLAARHAPPFEPGAKP
ECCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
WFVTGGVDARTLDAILETGARRVVVVRGIAEAADPGAAATALAERLRSA
EEEECCCCHHHHHHHHHCCCCEEEEEECHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA