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The map label for this gene is mscS [H]

Identifier: 157963531

GI number: 157963531

Start: 4524112

End: 4525728

Strand: Direct

Name: mscS [H]

Synonym: Spea_3719

Alternate gene names: 157963531

Gene position: 4524112-4525728 (Clockwise)

Preceding gene: 157963523

Following gene: 157963535

Centisome position: 87.43

GC content: 44.16

Gene sequence:

>1617_bases
ATGCATAAAATGCTCCCCCAAATTTTAATGCTTATTCTAAGCTTATGCTCATTTACAGCAGCGGCTGAGAATACAGAATT
AACTAATAATTTAAATAAAATCCAAAGCCTTATTGATAATGATGTCAAAGCACTACCAAGCACTAAAGGCGAAATTAAGA
GTTTTCTGGAATATCGCATAGGTCGCAATGCAGCTTTACTTCAGGACACCTTGAAGGAGCAAGTAGAAAAAACCGATCCA
GATTTCGCTTTCCTAAAGCCTTATATTGTTAAGCAGTTAGCCTTTACTAATGAAGTAGAAGCTTACTTTGAAAAGCAGAA
AGCCAGCCTGATGAGTCAAGTCGGTAGCGGTAGCGACAATAAAGTCATGGTTGGCCTAATGCAGATCCAGCGTGAGCAGG
ATAAGCTATTCACTGCAGAAAAAAGCCTATTAAACTGGGCCCAAAAGGCAGGAATCGATACCGACGCACAAACTAAAACC
TTTATCGCGACCCTGATTACTCGTGCCGATGATCTCAATAGCTTCGTCTACTACAACCAAGAGCGCCTCACTCAAGCTGT
AGCCGATGTTCAAGTTGCGGGTACCGATGTTAGCGCCGAGCAGAAGTCTCTTGTGGTAGACCTAAAAGAGCGCTTAGAAC
AGAGCTCTACTAGTCTTTCCACTACCATAGAGCTGCTCGATAGCTTGGACCAAGATACAGCTCTCTATAAGCAAACTCTG
TTTAGCATTTCTGGTGATATCACCCAAGATGTTTTGAACTTAGATGTTGCCAGCAGCTTATTGTCTGAGTGGATGACTAT
CGCTAAGAATCAAGCGATGGAAAATGGTGCCAGCATTCTATTTAAGCTAATTATCTTCTGCCTTATCCTGTTCCTATCTA
GCCTAGTCGGCAAGGTCGTTAAAAGTGTGGTGCAGAAGACGGTCAGCAACTCTAAGCTTAAATTTAGCATGCTGCTGCAA
GACTTCTTTATCTCACTCTCGGGTAAAGCGGTTTTCGCACTCGGTCTATTGATTGCCCTATCTCAACTTGGCTTTGAACT
GGCGCCACTGCTTGCCGGTTTCGGTATTGCCGGTGTAATTATTGGTTTTGCTTTGCAAGATACACTGTCTAATTTCGCTT
CAGGCATGATGATCTTGATCTACCGTCCGTTCGACGTCGGTGATCTTATCAACGCAGGCGGCGTCACCGGTCGTGTCAGT
CATATGAGCTTAGTATCGACCACGATTAAGACACTAGATAACCAGAGACTTATCGTGCCAAACAATAAGATCTGGGGCGA
CACCATCAATAACATTACCGTTGAGCATCAGCGTCGTGTGGATATGACCTTTGGTATTGGCTATGGCGATAATATCGAGC
ACGCAGAAAAAGTGCTTCACGATATCGTAATGGCGCACCCTCTAGTGCTTAAAGATCCAGAACCTATGATCAAGCTACAT
ACCTTAGGTGAGTCTTCGGTGGACTTTATTGTTCGTCCTTGGGCTAAGCCTGAAGATTACTGGGATGTATACTGGGATAT
CACTCGCACGGTTAAAATGCGCTTCGACGCCGAAGGCATTAGCATTCCGTTCCCACAGCGCGATGTGCATATCTATCAAA
CGGCTATAGAGAAGTAA

Upstream 100 bases:

>100_bases
AGTATTCGTTATTTTGTTTATCACTATTTCAATAACGAATCGACAGTTTTCCAATGCCGTAATATTATTACTTAATCTTG
GGAACTATCGGACACAGCAA

Downstream 100 bases:

>100_bases
CTCTTAAAGATAAAATGAAAAAAGCCCCGACAACCTAAGGTTGTCGGGGCTTTTTATTAGATTCGATAGCTAATACTTTT
AGGCTAATCCAACAATCTCG

Product: mechanosensitive ion channel protein MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MHKMLPQILMLILSLCSFTAAAENTELTNNLNKIQSLIDNDVKALPSTKGEIKSFLEYRIGRNAALLQDTLKEQVEKTDP
DFAFLKPYIVKQLAFTNEVEAYFEKQKASLMSQVGSGSDNKVMVGLMQIQREQDKLFTAEKSLLNWAQKAGIDTDAQTKT
FIATLITRADDLNSFVYYNQERLTQAVADVQVAGTDVSAEQKSLVVDLKERLEQSSTSLSTTIELLDSLDQDTALYKQTL
FSISGDITQDVLNLDVASSLLSEWMTIAKNQAMENGASILFKLIIFCLILFLSSLVGKVVKSVVQKTVSNSKLKFSMLLQ
DFFISLSGKAVFALGLLIALSQLGFELAPLLAGFGIAGVIIGFALQDTLSNFASGMMILIYRPFDVGDLINAGGVTGRVS
HMSLVSTTIKTLDNQRLIVPNNKIWGDTINNITVEHQRRVDMTFGIGYGDNIEHAEKVLHDIVMAHPLVLKDPEPMIKLH
TLGESSVDFIVRPWAKPEDYWDVYWDITRTVKMRFDAEGISIPFPQRDVHIYQTAIEK

Sequences:

>Translated_538_residues
MHKMLPQILMLILSLCSFTAAAENTELTNNLNKIQSLIDNDVKALPSTKGEIKSFLEYRIGRNAALLQDTLKEQVEKTDP
DFAFLKPYIVKQLAFTNEVEAYFEKQKASLMSQVGSGSDNKVMVGLMQIQREQDKLFTAEKSLLNWAQKAGIDTDAQTKT
FIATLITRADDLNSFVYYNQERLTQAVADVQVAGTDVSAEQKSLVVDLKERLEQSSTSLSTTIELLDSLDQDTALYKQTL
FSISGDITQDVLNLDVASSLLSEWMTIAKNQAMENGASILFKLIIFCLILFLSSLVGKVVKSVVQKTVSNSKLKFSMLLQ
DFFISLSGKAVFALGLLIALSQLGFELAPLLAGFGIAGVIIGFALQDTLSNFASGMMILIYRPFDVGDLINAGGVTGRVS
HMSLVSTTIKTLDNQRLIVPNNKIWGDTINNITVEHQRRVDMTFGIGYGDNIEHAEKVLHDIVMAHPLVLKDPEPMIKLH
TLGESSVDFIVRPWAKPEDYWDVYWDITRTVKMRFDAEGISIPFPQRDVHIYQTAIEK
>Mature_538_residues
MHKMLPQILMLILSLCSFTAAAENTELTNNLNKIQSLIDNDVKALPSTKGEIKSFLEYRIGRNAALLQDTLKEQVEKTDP
DFAFLKPYIVKQLAFTNEVEAYFEKQKASLMSQVGSGSDNKVMVGLMQIQREQDKLFTAEKSLLNWAQKAGIDTDAQTKT
FIATLITRADDLNSFVYYNQERLTQAVADVQVAGTDVSAEQKSLVVDLKERLEQSSTSLSTTIELLDSLDQDTALYKQTL
FSISGDITQDVLNLDVASSLLSEWMTIAKNQAMENGASILFKLIIFCLILFLSSLVGKVVKSVVQKTVSNSKLKFSMLLQ
DFFISLSGKAVFALGLLIALSQLGFELAPLLAGFGIAGVIIGFALQDTLSNFASGMMILIYRPFDVGDLINAGGVTGRVS
HMSLVSTTIKTLDNQRLIVPNNKIWGDTINNITVEHQRRVDMTFGIGYGDNIEHAEKVLHDIVMAHPLVLKDPEPMIKLH
TLGESSVDFIVRPWAKPEDYWDVYWDITRTVKMRFDAEGISIPFPQRDVHIYQTAIEK

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=278, Percent_Identity=32.3741007194245, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1786670, Length=204, Percent_Identity=28.4313725490196, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI2367355, Length=194, Percent_Identity=28.8659793814433, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787591, Length=207, Percent_Identity=27.536231884058, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 59884; Mature: 59884

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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ECCCCCCCEEEECCCCCCCHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure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ECCCCCCCEEEECCCCCCCHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]