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The map label for this gene is rumB [H]

Identifier: 157963447

GI number: 157963447

Start: 4425503

End: 4426579

Strand: Direct

Name: rumB [H]

Synonym: Spea_3635

Alternate gene names: 157963447

Gene position: 4425503-4426579 (Clockwise)

Preceding gene: 157963446

Following gene: 157963448

Centisome position: 85.52

GC content: 46.98

Gene sequence:

>1077_bases
ATGTCAGTGCAAGTCGCAGCTAAGTCGCAGGTGCTAAGCCAACTTCTGAGTGACTTCGAAGTTGAGCAGTGGCACGAGCC
AGTATTTGGCCCAGACAGTGGTTTTCGTAATAAAGCTAAAATGGTCGTACTCGGCGCGGCCCATCAGCCAATCCTTGGGA
TCGTGACCCCGACAGGTGAGCCAGTAAGCTTATGTGATTGCAACTTGTACCCAGAGGATATGCAGCTGTTGCTGCATCGC
TTAGAACAGTTTGTGCGCCAAGCAGGCATACCACCTTATAATGTCGACAAAGCAAAAGGTGAGCTTAAGTTTATTTTGCT
GACCCGCAGTCAAATCAAAGGCGAATACCTACTGCGCTTTGTACTCAAGTCTCACAAGTCTATCGAGCGTATTGAACGCG
AGCTACCAAAGCTATTAAGCGAGTACCCGCAGATCAAAGTGGTGTCGGTAAACATTCAGCCTGTGCATATGGCCATTCTC
GAAGGTGAAGAGGAGATCTTCTTAACCGAAGAGACCCGCTTGTCTGAGCAATTTAACGATGTGCCACTGTTTATTCGCCC
AAAAAGTTTCTTTCAGACTCATCCACAAATTGCAGCCAAACTTTACCAAACTGCTCGCGAGTGGGTTGCCGAGCTAAAAC
CTAGCTCATTGTGGGATCTTTTCTGTGGTGTGGGTGGCTTTGGTCTGCACTGCGCGTCAAAAACGATTCCATTGACTGGC
ATTGAAATCTCAAGCGAGGCTATTGCCTGCGCCAAAATATCTGCAGAAACCATGGGATTAACTCAAGTGGATTTCACCGC
GCTTGATTCGACCGGTTTTGCACAAGGTTGTGATGCCACTGACAAGCCAGATGTGGTCATAGTTAATCCGCCAAGACGTG
GCATAGGTGAAAGCTTGTGTCAGTCACTGTCAGATTTTGCTCCTAAAGCCATCTTGTACTCAAGCTGTAACCCACACACC
TTAGCTAAAGATTTAGCCAATATTCAGGGCTACCATATTCAAAAGGTACAGCTATTCGATATGTTCCCGCACACGGATCA
CTTCGAAGTCTTAGTAATGCTAGTTAAGCCTAGTTAA

Upstream 100 bases:

>100_bases
CAGCAAAATTGTCGTCCCAACGCTTTAGAGAACCCAATCAGTGAAATGTGCCCACTTTGATCAGCAGCAGTGTCTTTCTT
GCCGCCATATTAAACAATCC

Downstream 100 bases:

>100_bases
CTTCTGATTATCTCCTTGTAATGTCAATAGCACTCAGGCTTAGTTCATTTTGAAGCTAAGCCTACTTCTTCTTTTCTTCG
CTCGATTTTCAAACAACAAA

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MSVQVAAKSQVLSQLLSDFEVEQWHEPVFGPDSGFRNKAKMVVLGAAHQPILGIVTPTGEPVSLCDCNLYPEDMQLLLHR
LEQFVRQAGIPPYNVDKAKGELKFILLTRSQIKGEYLLRFVLKSHKSIERIERELPKLLSEYPQIKVVSVNIQPVHMAIL
EGEEEIFLTEETRLSEQFNDVPLFIRPKSFFQTHPQIAAKLYQTAREWVAELKPSSLWDLFCGVGGFGLHCASKTIPLTG
IEISSEAIACAKISAETMGLTQVDFTALDSTGFAQGCDATDKPDVVIVNPPRRGIGESLCQSLSDFAPKAILYSSCNPHT
LAKDLANIQGYHIQKVQLFDMFPHTDHFEVLVMLVKPS

Sequences:

>Translated_358_residues
MSVQVAAKSQVLSQLLSDFEVEQWHEPVFGPDSGFRNKAKMVVLGAAHQPILGIVTPTGEPVSLCDCNLYPEDMQLLLHR
LEQFVRQAGIPPYNVDKAKGELKFILLTRSQIKGEYLLRFVLKSHKSIERIERELPKLLSEYPQIKVVSVNIQPVHMAIL
EGEEEIFLTEETRLSEQFNDVPLFIRPKSFFQTHPQIAAKLYQTAREWVAELKPSSLWDLFCGVGGFGLHCASKTIPLTG
IEISSEAIACAKISAETMGLTQVDFTALDSTGFAQGCDATDKPDVVIVNPPRRGIGESLCQSLSDFAPKAILYSSCNPHT
LAKDLANIQGYHIQKVQLFDMFPHTDHFEVLVMLVKPS
>Mature_357_residues
SVQVAAKSQVLSQLLSDFEVEQWHEPVFGPDSGFRNKAKMVVLGAAHQPILGIVTPTGEPVSLCDCNLYPEDMQLLLHRL
EQFVRQAGIPPYNVDKAKGELKFILLTRSQIKGEYLLRFVLKSHKSIERIERELPKLLSEYPQIKVVSVNIQPVHMAILE
GEEEIFLTEETRLSEQFNDVPLFIRPKSFFQTHPQIAAKLYQTAREWVAELKPSSLWDLFCGVGGFGLHCASKTIPLTGI
EISSEAIACAKISAETMGLTQVDFTALDSTGFAQGCDATDKPDVVIVNPPRRGIGESLCQSLSDFAPKAILYSSCNPHTL
AKDLANIQGYHIQKVQLFDMFPHTDHFEVLVMLVKPS

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI269784680, Length=216, Percent_Identity=26.8518518518519, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI269784676, Length=216, Percent_Identity=26.8518518518519, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI20149688, Length=216, Percent_Identity=26.8518518518519, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI34222389, Length=189, Percent_Identity=26.984126984127, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI51173878, Length=189, Percent_Identity=26.984126984127, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI269784678, Length=216, Percent_Identity=26.8518518518519, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787083, Length=353, Percent_Identity=57.5070821529745, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1789148, Length=186, Percent_Identity=30.6451612903226, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790403, Length=166, Percent_Identity=28.3132530120482, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24666579, Length=404, Percent_Identity=24.2574257425743, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 39904; Mature: 39772

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVQVAAKSQVLSQLLSDFEVEQWHEPVFGPDSGFRNKAKMVVLGAAHQPILGIVTPTGE
CCEEHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCC
PVSLCDCNLYPEDMQLLLHRLEQFVRQAGIPPYNVDKAKGELKFILLTRSQIKGEYLLRF
CEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHH
VLKSHKSIERIERELPKLLSEYPQIKVVSVNIQPVHMAILEGEEEIFLTEETRLSEQFND
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEEECCCCEEEEECHHHHHHHCCC
VPLFIRPKSFFQTHPQIAAKLYQTAREWVAELKPSSLWDLFCGVGGFGLHCASKTIPLTG
CCEEECCHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEECCCCCCEEC
IEISSEAIACAKISAETMGLTQVDFTALDSTGFAQGCDATDKPDVVIVNPPRRGIGESLC
EEECCCCEEEEEECHHHCCCEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHH
QSLSDFAPKAILYSSCNPHTLAKDLANIQGYHIQKVQLFDMFPHTDHFEVLVMLVKPS
HHHHHHCCCEEEECCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEEEECCC
>Mature Secondary Structure 
SVQVAAKSQVLSQLLSDFEVEQWHEPVFGPDSGFRNKAKMVVLGAAHQPILGIVTPTGE
CEEHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCC
PVSLCDCNLYPEDMQLLLHRLEQFVRQAGIPPYNVDKAKGELKFILLTRSQIKGEYLLRF
CEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHH
VLKSHKSIERIERELPKLLSEYPQIKVVSVNIQPVHMAILEGEEEIFLTEETRLSEQFND
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEEECCCCEEEEECHHHHHHHCCC
VPLFIRPKSFFQTHPQIAAKLYQTAREWVAELKPSSLWDLFCGVGGFGLHCASKTIPLTG
CCEEECCHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEECCCCCCEEC
IEISSEAIACAKISAETMGLTQVDFTALDSTGFAQGCDATDKPDVVIVNPPRRGIGESLC
EEECCCCEEEEEECHHHCCCEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHH
QSLSDFAPKAILYSSCNPHTLAKDLANIQGYHIQKVQLFDMFPHTDHFEVLVMLVKPS
HHHHHHCCCEEEECCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA