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The map label for this gene is rpoN [H]

Identifier: 157963430

GI number: 157963430

Start: 4407138

End: 4408601

Strand: Direct

Name: rpoN [H]

Synonym: Spea_3616

Alternate gene names: 157963430

Gene position: 4407138-4408601 (Clockwise)

Preceding gene: 157963429

Following gene: 157963431

Centisome position: 85.17

GC content: 45.22

Gene sequence:

>1464_bases
ATGAAAGCGTCACTCCAGCTCAAAATGGGTCAGCAGCTCACCATGACTCCCCAGTTACAACAGGCCATTCGCCTGTTACA
ACTGTCTTCTTTAGAGCTGCAACAAGAGATCCAACAAGCATTAGACTCTAATCCGCTATTGGAATTAGATGAAGAGCAGT
CAGACGGTGATTTAGGCGACAATGAAGACCAATCATCAAACGATGCCAATAGTGACCTCATCCAAGATAGCGCTAGCGTT
GAAACCTCCGATGCCCTAACCCAAGACTCTATGCCAGATGATCTGCCAATGGACACCACTTGGGATGAAGTTTATACCGT
CTCGTCAAACTCCAGCTCTGGAGTTAGCCGTGAAGACGATATGCCATTCCAAGGTGAAACCAGCGAAGGCTTATATGAAC
ATTTAGAGTGGCAGAAAAACCTCACACCGTTTTCAGATAACGATCTTGCAATCGCCACCGCGATTATCGACGCCATTGAC
GAACGTGGCTACCTTACCCAAAGCTGCGACGATATTTTAGAAGCTATGGGCGATCCTGAAACCGAGCTTGATGAAGTCGA
AGCGGTATTAAAGCGCATCCAGCATTTTGACCCTATTGGTGTCGCCGCGCGTAATTTGAGTGAATGCTTGCTTATCCAGC
TGGCACAATATGCTGCAGAAACCCCGCATATCGACAATGCCAGATTACTGATAAAAGATTATTTAGACTTAATTGCAGGC
CGCGATTTTAGGCAGTTAATGCGTAAAACCAAGCTTAAAGAAGATGAACTACGTGATGCGATAACACTTATTCAGACCCT
TAATCCAAGACCAGGGCTGGCGATTGCTCGCAGCAAAGACGAGTATGTTATCCCTGACGTTTCCGTGTGCAAGAAAAAAG
GTCGCTGGGTGGTTGAGCTTAATCCTGACTATATGCCTAAGATTAATGTTAACCAACATTATGCCTCTATGGCGCGTAGC
ACCACTAATCAGGCCGATGGTCAATTTATTCGTGGTCATCTGCAGGAAGCTAAGTGGTTTATTAAGAGTCTCGAGAGTCG
TAATGACACCTTATTGAAAGTAACCAACTGTATCGTTAAATTCCAGCAAGGCTTCTTTGAGTATGGCGAAGAAGCCATGA
AGCCTATGGTACTCAATGATATTGCCGAAGCAGTTGAGATGCACGAGTCGACCATTTCACGGGTGACTACCCAAAAATAT
ATGCACACTCCAAGAGGTATTTTTGAACTAAAGTACTTCTTCTCAAGCCATGTCGGTACCGACGATGGTGGGGAGTGTTC
ATCGACCGCAATCAGAGCGTTTATTAAGAAGTTAGTCGCCGCAGAAAACCAGAAGAAGCCATTAAGCGATAGCAAGATGG
CACAACTTTTGGCAGAACAAGGGATTAAAGTTGCTAGACGTACGATAGCAAAATATCGAGAAGCGATGCTGATCCCCCCC
TCAAACCAACGTAAGAGTTTATAA

Upstream 100 bases:

>100_bases
GATTTACTTCCAAATAAAATCAAAGTGTTGAGTTTTCAAGCTACCTTCGCCAGTTCAGCGAGCTTTAACGTTTAACTACA
GTGTAGGGAATAGCGGTAAA

Downstream 100 bases:

>100_bases
CTCAAAATTAGGCACTTGGAGGATCGATATATGCAAATCAACCTGACTGGACACCACGTCGAAATCACCGATTCACTGCG
CGGTTATGTAGAAGATAAAT

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MKASLQLKMGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDSNPLLELDEEQSDGDLGDNEDQSSNDANSDLIQDSASV
ETSDALTQDSMPDDLPMDTTWDEVYTVSSNSSSGVSREDDMPFQGETSEGLYEHLEWQKNLTPFSDNDLAIATAIIDAID
ERGYLTQSCDDILEAMGDPETELDEVEAVLKRIQHFDPIGVAARNLSECLLIQLAQYAAETPHIDNARLLIKDYLDLIAG
RDFRQLMRKTKLKEDELRDAITLIQTLNPRPGLAIARSKDEYVIPDVSVCKKKGRWVVELNPDYMPKINVNQHYASMARS
TTNQADGQFIRGHLQEAKWFIKSLESRNDTLLKVTNCIVKFQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKY
MHTPRGIFELKYFFSSHVGTDDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAQLLAEQGIKVARRTIAKYREAMLIPP
SNQRKSL

Sequences:

>Translated_487_residues
MKASLQLKMGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDSNPLLELDEEQSDGDLGDNEDQSSNDANSDLIQDSASV
ETSDALTQDSMPDDLPMDTTWDEVYTVSSNSSSGVSREDDMPFQGETSEGLYEHLEWQKNLTPFSDNDLAIATAIIDAID
ERGYLTQSCDDILEAMGDPETELDEVEAVLKRIQHFDPIGVAARNLSECLLIQLAQYAAETPHIDNARLLIKDYLDLIAG
RDFRQLMRKTKLKEDELRDAITLIQTLNPRPGLAIARSKDEYVIPDVSVCKKKGRWVVELNPDYMPKINVNQHYASMARS
TTNQADGQFIRGHLQEAKWFIKSLESRNDTLLKVTNCIVKFQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKY
MHTPRGIFELKYFFSSHVGTDDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAQLLAEQGIKVARRTIAKYREAMLIPP
SNQRKSL
>Mature_487_residues
MKASLQLKMGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDSNPLLELDEEQSDGDLGDNEDQSSNDANSDLIQDSASV
ETSDALTQDSMPDDLPMDTTWDEVYTVSSNSSSGVSREDDMPFQGETSEGLYEHLEWQKNLTPFSDNDLAIATAIIDAID
ERGYLTQSCDDILEAMGDPETELDEVEAVLKRIQHFDPIGVAARNLSECLLIQLAQYAAETPHIDNARLLIKDYLDLIAG
RDFRQLMRKTKLKEDELRDAITLIQTLNPRPGLAIARSKDEYVIPDVSVCKKKGRWVVELNPDYMPKINVNQHYASMARS
TTNQADGQFIRGHLQEAKWFIKSLESRNDTLLKVTNCIVKFQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKY
MHTPRGIFELKYFFSSHVGTDDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAQLLAEQGIKVARRTIAKYREAMLIPP
SNQRKSL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor recognizes a DNA element, designated as region A upstream of the pressure-regulated operon [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=489, Percent_Identity=62.7811860940695, Blast_Score=629, Evalue=0.0,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54821; Mature: 54821

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASLQLKMGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDSNPLLELDEEQSDGDLGD
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
NEDQSSNDANSDLIQDSASVETSDALTQDSMPDDLPMDTTWDEVYTVSSNSSSGVSREDD
CCCCCCCCCCHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCHHHEEEECCCCCCCCCCCCC
MPFQGETSEGLYEHLEWQKNLTPFSDNDLAIATAIIDAIDERGYLTQSCDDILEAMGDPE
CCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
TELDEVEAVLKRIQHFDPIGVAARNLSECLLIQLAQYAAETPHIDNARLLIKDYLDLIAG
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
RDFRQLMRKTKLKEDELRDAITLIQTLNPRPGLAIARSKDEYVIPDVSVCKKKGRWVVEL
CHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCHHHHHHCCCEEEEE
NPDYMPKINVNQHYASMARSTTNQADGQFIRGHLQEAKWFIKSLESRNDTLLKVTNCIVK
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFELKYFFSSHVGT
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
DDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAQLLAEQGIKVARRTIAKYREAMLIPP
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
SNQRKSL
CCCCCCC
>Mature Secondary Structure
MKASLQLKMGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDSNPLLELDEEQSDGDLGD
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
NEDQSSNDANSDLIQDSASVETSDALTQDSMPDDLPMDTTWDEVYTVSSNSSSGVSREDD
CCCCCCCCCCHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCHHHEEEECCCCCCCCCCCCC
MPFQGETSEGLYEHLEWQKNLTPFSDNDLAIATAIIDAIDERGYLTQSCDDILEAMGDPE
CCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
TELDEVEAVLKRIQHFDPIGVAARNLSECLLIQLAQYAAETPHIDNARLLIKDYLDLIAG
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
RDFRQLMRKTKLKEDELRDAITLIQTLNPRPGLAIARSKDEYVIPDVSVCKKKGRWVVEL
CHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCHHHHHHCCCEEEEE
NPDYMPKINVNQHYASMARSTTNQADGQFIRGHLQEAKWFIKSLESRNDTLLKVTNCIVK
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFELKYFFSSHVGT
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
DDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAQLLAEQGIKVARRTIAKYREAMLIPP
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
SNQRKSL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10760597 [H]