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The map label for this gene is fhlA [H]

Identifier: 157963136

GI number: 157963136

Start: 4060373

End: 4061827

Strand: Direct

Name: fhlA [H]

Synonym: Spea_3320

Alternate gene names: 157963136

Gene position: 4060373-4061827 (Clockwise)

Preceding gene: 157963135

Following gene: 157963137

Centisome position: 78.47

GC content: 48.66

Gene sequence:

>1455_bases
ATGTCTGCCATAAACCTTGAAAAGAAAACCAGTCACGATGATTTTCTTCCCGAGAACCAACTCATTCTGAACGCGGTCAG
CGAAGGCATTTACGGTTTCGATTTAGCGGGTAATGCGGTTTTTATTAATCCCGCTGCCGAGCGAATGACAGGCTGGCAAT
CCCATGAGTTACTAGGCAAGAATATCCATGATTGTCATCACCATAGCCACAGCGATGGTAGCCATTACCATAAAGATGAC
TGCCCGATTTATAACACCTTAATCGACGGCACTGCCCGTGAGATCTCTCATGAAGTCTTCTGGCGCAAAGACGGCACCAG
CTTTCCGGTGCATTACACCTCTACCCCAGTGTACAAAGATGGCGTATTAATTGGCGTGGTCGCGATATTTCGTGACATCA
GTATTCAGAAGCAAACTGAGCATTCATTACGTCAGGCGCTCGAGCAAGTACAAGCCCTATCGGAAAAGCTGTCGTTTGAA
AACCAATACCTACAACTAGAACTGGCAGATAGAACTGGGGATGTTGAGATCTCAGGTAGCAGCCAGCCGATGAATCAGCT
AATTCAGCAAATTAAACTCGTTTCCAATACCGAGAGTACGGTATTGATCAGTGGCGAAAATGGCACAGGCAAAGAGTTAG
TCGCCCGCAACTTACATCGCTTGAGTAACCGCGCAGATAAGCCATTAATCAGCGTCAACTGCGCCGCCTTTTCCAGCGCC
TTGTTAGAGAGTGAACTATTTGGCCATGAAAAAGGGGCATACACAGGTGCCACCAGCCGCCGTAAAGGGCGATTTGAACT
GGCTCACCAAGGCACGCTGTTTCTAGACGAAGTAGCCGAGCTCAGCCTAGAAGCCCAGTCTAAACTACTGCGGGTGATCC
AGGAGCAGGAATTTGAGCGCGTAGGCAGTAGCCAAACCATCAAGGTAGACATTCGCCTCATTACCGCAACTCACCATGAT
CTGCTTAAGCGTGTAGAACAAGGCTTGTTCCGTATGGATCTCTATTATCGCCTCAATGTGTTTCCCATCGTGGTACCGCC
ATTGAGGCTACGATTAAGCGATATCCCTGAATTAGTCAGCCATCTCATTCAAGACTTAAACCGCAAGCTAGGTAAGAAGG
TCACCCAAGTTAGCCACAAAGGCATGACCAAACTCATGCAACACAGTTGGCCGGGAAACGTACGTGAGCTGCAAAATGTC
TTGGAGCGTGAGATGATTTTATCCCAAGGGCCAGTACTGGAGTTCTCACAGCTACAGTTTGCAGATCAAGCTGAAAATCC
TCCCCTAGCGACCAGCCAAACCTTACAAGAGGTGGAACGCCAACATATTTTGCATGTACTCAAGCAAACCCAATGGCGGA
TATCAGGGGCAAGCGGCGCAGCGGCATTGTTAGGCCTTCCACCGAGCACCTTACGTTCACGGATGAATAAATTGGCCATT
TCTCGCCCCGTCTAA

Upstream 100 bases:

>100_bases
AATAAAAACAATAAGGTCACGTTATTTCGTGGCCTTATTTTTTTAGCTATCACTTTTGCGTATACTGTGGCGACAAATTG
CTGCGTACACAGGGCTCAAC

Downstream 100 bases:

>100_bases
GCTAACGCCGCCTTAGATAGCACCGCCTTCACTAGACCTTAGCCTAACTCGTAGGCTGTCTATCTGGCTCTAGCATCCGG
CTCTCGATCCCTGAGCTCAA

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSAINLEKKTSHDDFLPENQLILNAVSEGIYGFDLAGNAVFINPAAERMTGWQSHELLGKNIHDCHHHSHSDGSHYHKDD
CPIYNTLIDGTAREISHEVFWRKDGTSFPVHYTSTPVYKDGVLIGVVAIFRDISIQKQTEHSLRQALEQVQALSEKLSFE
NQYLQLELADRTGDVEISGSSQPMNQLIQQIKLVSNTESTVLISGENGTGKELVARNLHRLSNRADKPLISVNCAAFSSA
LLESELFGHEKGAYTGATSRRKGRFELAHQGTLFLDEVAELSLEAQSKLLRVIQEQEFERVGSSQTIKVDIRLITATHHD
LLKRVEQGLFRMDLYYRLNVFPIVVPPLRLRLSDIPELVSHLIQDLNRKLGKKVTQVSHKGMTKLMQHSWPGNVRELQNV
LEREMILSQGPVLEFSQLQFADQAENPPLATSQTLQEVERQHILHVLKQTQWRISGASGAAALLGLPPSTLRSRMNKLAI
SRPV

Sequences:

>Translated_484_residues
MSAINLEKKTSHDDFLPENQLILNAVSEGIYGFDLAGNAVFINPAAERMTGWQSHELLGKNIHDCHHHSHSDGSHYHKDD
CPIYNTLIDGTAREISHEVFWRKDGTSFPVHYTSTPVYKDGVLIGVVAIFRDISIQKQTEHSLRQALEQVQALSEKLSFE
NQYLQLELADRTGDVEISGSSQPMNQLIQQIKLVSNTESTVLISGENGTGKELVARNLHRLSNRADKPLISVNCAAFSSA
LLESELFGHEKGAYTGATSRRKGRFELAHQGTLFLDEVAELSLEAQSKLLRVIQEQEFERVGSSQTIKVDIRLITATHHD
LLKRVEQGLFRMDLYYRLNVFPIVVPPLRLRLSDIPELVSHLIQDLNRKLGKKVTQVSHKGMTKLMQHSWPGNVRELQNV
LEREMILSQGPVLEFSQLQFADQAENPPLATSQTLQEVERQHILHVLKQTQWRISGASGAAALLGLPPSTLRSRMNKLAI
SRPV
>Mature_483_residues
SAINLEKKTSHDDFLPENQLILNAVSEGIYGFDLAGNAVFINPAAERMTGWQSHELLGKNIHDCHHHSHSDGSHYHKDDC
PIYNTLIDGTAREISHEVFWRKDGTSFPVHYTSTPVYKDGVLIGVVAIFRDISIQKQTEHSLRQALEQVQALSEKLSFEN
QYLQLELADRTGDVEISGSSQPMNQLIQQIKLVSNTESTVLISGENGTGKELVARNLHRLSNRADKPLISVNCAAFSSAL
LESELFGHEKGAYTGATSRRKGRFELAHQGTLFLDEVAELSLEAQSKLLRVIQEQEFERVGSSQTIKVDIRLITATHHDL
LKRVEQGLFRMDLYYRLNVFPIVVPPLRLRLSDIPELVSHLIQDLNRKLGKKVTQVSHKGMTKLMQHSWPGNVRELQNVL
EREMILSQGPVLEFSQLQFADQAENPPLATSQTLQEVERQHILHVLKQTQWRISGASGAAALLGLPPSTLRSRMNKLAIS
RPV

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=340, Percent_Identity=48.2352941176471, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI87082117, Length=350, Percent_Identity=47.1428571428571, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI1790437, Length=302, Percent_Identity=48.3443708609272, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1788550, Length=316, Percent_Identity=42.4050632911392, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1788905, Length=279, Percent_Identity=42.2939068100358, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1790299, Length=235, Percent_Identity=47.2340425531915, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=46.8085106382979, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1789233, Length=228, Percent_Identity=46.4912280701754, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1786524, Length=318, Percent_Identity=39.622641509434, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI87081872, Length=226, Percent_Identity=46.0176991150442, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI1787583, Length=230, Percent_Identity=38.2608695652174, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87081858, Length=287, Percent_Identity=35.5400696864112, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789828, Length=293, Percent_Identity=30.0341296928328, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54366; Mature: 54234

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAINLEKKTSHDDFLPENQLILNAVSEGIYGFDLAGNAVFINPAAERMTGWQSHELLGK
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCEEEECCHHHHHCCCCHHHHHCC
NIHDCHHHSHSDGSHYHKDDCPIYNTLIDGTAREISHEVFWRKDGTSFPVHYTSTPVYKD
CHHHHHCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHEECCCCCCCEEECCCCCCCC
GVLIGVVAIFRDISIQKQTEHSLRQALEQVQALSEKLSFENQYLQLELADRTGDVEISGS
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEECCC
SQPMNQLIQQIKLVSNTESTVLISGENGTGKELVARNLHRLSNRADKPLISVNCAAFSSA
CCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHH
LLESELFGHEKGAYTGATSRRKGRFELAHQGTLFLDEVAELSLEAQSKLLRVIQEQEFER
HHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGSSQTIKVDIRLITATHHDLLKRVEQGLFRMDLYYRLNVFPIVVPPLRLRLSDIPELVS
CCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHHHHHHHH
HLIQDLNRKLGKKVTQVSHKGMTKLMQHSWPGNVRELQNVLEREMILSQGPVLEFSQLQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
ADQAENPPLATSQTLQEVERQHILHVLKQTQWRISGASGAAALLGLPPSTLRSRMNKLAI
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCHHHHHHHHHHHHC
SRPV
CCCC
>Mature Secondary Structure 
SAINLEKKTSHDDFLPENQLILNAVSEGIYGFDLAGNAVFINPAAERMTGWQSHELLGK
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCEEEECCHHHHHCCCCHHHHHCC
NIHDCHHHSHSDGSHYHKDDCPIYNTLIDGTAREISHEVFWRKDGTSFPVHYTSTPVYKD
CHHHHHCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHEECCCCCCCEEECCCCCCCC
GVLIGVVAIFRDISIQKQTEHSLRQALEQVQALSEKLSFENQYLQLELADRTGDVEISGS
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEECCC
SQPMNQLIQQIKLVSNTESTVLISGENGTGKELVARNLHRLSNRADKPLISVNCAAFSSA
CCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHH
LLESELFGHEKGAYTGATSRRKGRFELAHQGTLFLDEVAELSLEAQSKLLRVIQEQEFER
HHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGSSQTIKVDIRLITATHHDLLKRVEQGLFRMDLYYRLNVFPIVVPPLRLRLSDIPELVS
CCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHHHHHHHH
HLIQDLNRKLGKKVTQVSHKGMTKLMQHSWPGNVRELQNVLEREMILSQGPVLEFSQLQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
ADQAENPPLATSQTLQEVERQHILHVLKQTQWRISGASGAAALLGLPPSTLRSRMNKLAI
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCHHHHHHHHHHHHC
SRPV
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]