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The map label for this gene is mrpA [H]

Identifier: 157963103

GI number: 157963103

Start: 4028175

End: 4029875

Strand: Direct

Name: mrpA [H]

Synonym: Spea_3287

Alternate gene names: 157963103

Gene position: 4028175-4029875 (Clockwise)

Preceding gene: 157963102

Following gene: 157963104

Centisome position: 77.85

GC content: 50.15

Gene sequence:

>1701_bases
ATGTGGATGCTTGAGCTACCGCCCTTTACCCTATTCTTTATAGGTGGGCTTATTGCTGCAATGACCCGGGGCACACTGCG
CGGCGCAATAATGGTTGCCATTCCTGTTATTAGCGCCATTCACCTGTGGACAGTGCCTGAAGGTATTCATCTACAGCTGA
CGTTTTTAGATTATGAGTTGGTGCCATACCGCGCTGATAAACTGAGCCTGATGTTTGGCTATGTATTTCATATCGCCGCA
TTCATCTCCATTATCTACTCGCTGCATGTCAAAGATAGCGTACAACAGGTCGCAGCCATGCTCTATGCAGGTAGTGCGTT
GGGCGCTGTATTTGCGGGCGATCTACTGACTCTATTTATATTCTGGGAGCTACTCGCCTTTACCTCAGTGTTTTTAATCT
GGGCGCGCAGAACGACCCGCGCTTATCATGCAGGCATGCGCTACCTGATCATTCAGGTCCTATCCGGTGTCATCTTGCTA
GTAGGCGCACTATTTTACGCCGCTGAAACAGGCTCGCTATCTTTTGAGTATATTGGCCTAGATAGTATCGCTGGCTGGCT
GATCTTTATCGCCTTTGGTATTAAATGTGCTTTTCCCTTTGCCCATACTTGGCTCACCGACGCCTACCCTGAAGCCACAC
CAACGGGTACCGTTATTCTCAGTGCATTTACCACTAAGGTAGCCGTTTACGCACTGGCGCGCGCCTACCCAGGCACCGAG
CTATTGATATACATTGGCGCAGCCATGACCTGCTTCCCGATCTTCTTTGCGGTGATTGAAAATGATCTTCGCAGAGTCTT
GGCCTATAGCCTGATAAACCAGGTGGGCTTTATGGTGGTAGGTATAGGTATTGGTACTGCACTGGCAATCAACGGCGCAA
TAGCTCATGCCTTCAACGATGTGATCTTTAAAGGCTTGCTGTTTATGAGCATGGGCGCAGTGCTGCATATGACTGGCAGA
ATCAACGGCTCGGATCTGGGCGGCCTGTATAAAACCATGCCCAAGACCACGATACTATGTATTATCGGCGCCGCCTCTAT
CTCAGCCTTCCCACTGTTTAGCGGCTTTGTCAGTAAATCAATGGTGATGTCTGCCGCGCTTGAAACCGGTCATGACTGGG
TCTGGTTGATGTTACTGTTCGCCTCTGCGGGTGTGTTCCACCATGCAGGGATCAAGATCCCCTACTTTGCATTTTTCGCC
CACGACTCGGGGCTGCGCGCCAGCGATCCGCCGCGCAATATGTTATTCGCCATGTTTATCGCCGCCAGCCTGTGTATTGC
CATCGGTATCTATCCTGCAGCGCTGTACTCATTGCTGCCATATGATACGGGTTACAACCCTTATGATGCGACCCACGTAT
TGGCGCAGACTCAGTTGCTGATGTTCTCAGCCCTGGCGTTTGTTTGGCTAAATCTCAGAGGCATGTATCCTCCCGAATTG
CGTTCAACCAACTTGGATGTGGACTGGGTTTATCGCCGCGCTATTCCCAACGTGCTACAAAAACTGTTCGCGGTAATATG
GAAAGCTGATGGGGCGCTGCGCCAAGCTATACGAGGCAAACTAAGCCAATACCAGTCCATTATTGCCAACAAACATAAAG
GCTTAGGAGGCTTGCTATCAGGCTCATATCCTTCGGGTAATATGGTGCTATGGGTAGCCGTGCTGCTAGCGTCTTATCTG
TTTATGGGTTTTATTGGCTAG

Upstream 100 bases:

>100_bases
AGCAACATTCATTACCAAAACACTTCAGAAAATGCTGCAGATGAAAACACTCAAAAGGGTGTCCCCCTGCTAGCAAAAAA
CAACACTAGAGGTGGTCACC

Downstream 100 bases:

>100_bases
GTTTGAGTGAGGAAAGATATGGGGTCAAAGTCTGACCCCATTTATCATACACCCACGCTTGTTAACCACTTGTAACACTA
ATAGCAATGGCTTATCTTTA

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MWMLELPPFTLFFIGGLIAAMTRGTLRGAIMVAIPVISAIHLWTVPEGIHLQLTFLDYELVPYRADKLSLMFGYVFHIAA
FISIIYSLHVKDSVQQVAAMLYAGSALGAVFAGDLLTLFIFWELLAFTSVFLIWARRTTRAYHAGMRYLIIQVLSGVILL
VGALFYAAETGSLSFEYIGLDSIAGWLIFIAFGIKCAFPFAHTWLTDAYPEATPTGTVILSAFTTKVAVYALARAYPGTE
LLIYIGAAMTCFPIFFAVIENDLRRVLAYSLINQVGFMVVGIGIGTALAINGAIAHAFNDVIFKGLLFMSMGAVLHMTGR
INGSDLGGLYKTMPKTTILCIIGAASISAFPLFSGFVSKSMVMSAALETGHDWVWLMLLFASAGVFHHAGIKIPYFAFFA
HDSGLRASDPPRNMLFAMFIAASLCIAIGIYPAALYSLLPYDTGYNPYDATHVLAQTQLLMFSALAFVWLNLRGMYPPEL
RSTNLDVDWVYRRAIPNVLQKLFAVIWKADGALRQAIRGKLSQYQSIIANKHKGLGGLLSGSYPSGNMVLWVAVLLASYL
FMGFIG

Sequences:

>Translated_566_residues
MWMLELPPFTLFFIGGLIAAMTRGTLRGAIMVAIPVISAIHLWTVPEGIHLQLTFLDYELVPYRADKLSLMFGYVFHIAA
FISIIYSLHVKDSVQQVAAMLYAGSALGAVFAGDLLTLFIFWELLAFTSVFLIWARRTTRAYHAGMRYLIIQVLSGVILL
VGALFYAAETGSLSFEYIGLDSIAGWLIFIAFGIKCAFPFAHTWLTDAYPEATPTGTVILSAFTTKVAVYALARAYPGTE
LLIYIGAAMTCFPIFFAVIENDLRRVLAYSLINQVGFMVVGIGIGTALAINGAIAHAFNDVIFKGLLFMSMGAVLHMTGR
INGSDLGGLYKTMPKTTILCIIGAASISAFPLFSGFVSKSMVMSAALETGHDWVWLMLLFASAGVFHHAGIKIPYFAFFA
HDSGLRASDPPRNMLFAMFIAASLCIAIGIYPAALYSLLPYDTGYNPYDATHVLAQTQLLMFSALAFVWLNLRGMYPPEL
RSTNLDVDWVYRRAIPNVLQKLFAVIWKADGALRQAIRGKLSQYQSIIANKHKGLGGLLSGSYPSGNMVLWVAVLLASYL
FMGFIG
>Mature_566_residues
MWMLELPPFTLFFIGGLIAAMTRGTLRGAIMVAIPVISAIHLWTVPEGIHLQLTFLDYELVPYRADKLSLMFGYVFHIAA
FISIIYSLHVKDSVQQVAAMLYAGSALGAVFAGDLLTLFIFWELLAFTSVFLIWARRTTRAYHAGMRYLIIQVLSGVILL
VGALFYAAETGSLSFEYIGLDSIAGWLIFIAFGIKCAFPFAHTWLTDAYPEATPTGTVILSAFTTKVAVYALARAYPGTE
LLIYIGAAMTCFPIFFAVIENDLRRVLAYSLINQVGFMVVGIGIGTALAINGAIAHAFNDVIFKGLLFMSMGAVLHMTGR
INGSDLGGLYKTMPKTTILCIIGAASISAFPLFSGFVSKSMVMSAALETGHDWVWLMLLFASAGVFHHAGIKIPYFAFFA
HDSGLRASDPPRNMLFAMFIAASLCIAIGIYPAALYSLLPYDTGYNPYDATHVLAQTQLLMFSALAFVWLNLRGMYPPEL
RSTNLDVDWVYRRAIPNVLQKLFAVIWKADGALRQAIRGKLSQYQSIIANKHKGLGGLLSGSYPSGNMVLWVAVLLASYL
FMGFIG

Specific function: Mnh complex is a Na(+)Li(+)/H(+) antiporter involved in Na(+) and/or Li(+) excretion and Na(+) resistance. Na(+)/H(+) antiport consumes a transmembrane electrical potential, and is thus inferred to be electrogenic. Does not transport K(+), Ca(2+) or Mg(2+

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=351, Percent_Identity=26.2108262108262, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI1788827, Length=353, Percent_Identity=27.1954674220963, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788614, Length=294, Percent_Identity=29.5918367346939, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788831, Length=195, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1788829, Length=415, Percent_Identity=24.8192771084337, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788613, Length=336, Percent_Identity=23.8095238095238, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI2367154, Length=336, Percent_Identity=22.9166666666667, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI145693160, Length=377, Percent_Identity=24.1379310344828, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 61968; Mature: 61968

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWMLELPPFTLFFIGGLIAAMTRGTLRGAIMVAIPVISAIHLWTVPEGIHLQLTFLDYEL
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEEEEE
VPYRADKLSLMFGYVFHIAAFISIIYSLHVKDSVQQVAAMLYAGSALGAVFAGDLLTLFI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
FWELLAFTSVFLIWARRTTRAYHAGMRYLIIQVLSGVILLVGALFYAAETGSLSFEYIGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECH
DSIAGWLIFIAFGIKCAFPFAHTWLTDAYPEATPTGTVILSAFTTKVAVYALARAYPGTE
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
LLIYIGAAMTCFPIFFAVIENDLRRVLAYSLINQVGFMVVGIGIGTALAINGAIAHAFND
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHH
VIFKGLLFMSMGAVLHMTGRINGSDLGGLYKTMPKTTILCIIGAASISAFPLFSGFVSKS
HHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHH
MVMSAALETGHDWVWLMLLFASAGVFHHAGIKIPYFAFFAHDSGLRASDPPRNMLFAMFI
HHHHHHHHCCCHHHHHHHHHHHCCHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
AASLCIAIGIYPAALYSLLPYDTGYNPYDATHVLAQTQLLMFSALAFVWLNLRGMYPPEL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RSTNLDVDWVYRRAIPNVLQKLFAVIWKADGALRQAIRGKLSQYQSIIANKHKGLGGLLS
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GSYPSGNMVLWVAVLLASYLFMGFIG
CCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWMLELPPFTLFFIGGLIAAMTRGTLRGAIMVAIPVISAIHLWTVPEGIHLQLTFLDYEL
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEEEEE
VPYRADKLSLMFGYVFHIAAFISIIYSLHVKDSVQQVAAMLYAGSALGAVFAGDLLTLFI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
FWELLAFTSVFLIWARRTTRAYHAGMRYLIIQVLSGVILLVGALFYAAETGSLSFEYIGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECH
DSIAGWLIFIAFGIKCAFPFAHTWLTDAYPEATPTGTVILSAFTTKVAVYALARAYPGTE
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
LLIYIGAAMTCFPIFFAVIENDLRRVLAYSLINQVGFMVVGIGIGTALAINGAIAHAFND
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHH
VIFKGLLFMSMGAVLHMTGRINGSDLGGLYKTMPKTTILCIIGAASISAFPLFSGFVSKS
HHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHH
MVMSAALETGHDWVWLMLLFASAGVFHHAGIKIPYFAFFAHDSGLRASDPPRNMLFAMFI
HHHHHHHHCCCHHHHHHHHHHHCCHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
AASLCIAIGIYPAALYSLLPYDTGYNPYDATHVLAQTQLLMFSALAFVWLNLRGMYPPEL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RSTNLDVDWVYRRAIPNVLQKLFAVIWKADGALRQAIRGKLSQYQSIIANKHKGLGGLLS
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GSYPSGNMVLWVAVLLASYLFMGFIG
CCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]