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The map label for this gene is nuoN [H]

Identifier: 157963100

GI number: 157963100

Start: 4024814

End: 4026292

Strand: Direct

Name: nuoN [H]

Synonym: Spea_3284

Alternate gene names: 157963100

Gene position: 4024814-4026292 (Clockwise)

Preceding gene: 157963099

Following gene: 157963101

Centisome position: 77.78

GC content: 50.85

Gene sequence:

>1479_bases
GTGTTAGCACATCTGCCTATTTTACAAGTCATTGTGCCACTGATGGCGGCACCGCTATGCTGGTTTCTAGACCGCTCCAG
ACTCGTGTGGCTGTTCGCCCTCTTAGTGAGCGGTTTTTCCTGCCTAACCAGCATAGCCCTACTGCAACAAGTGATGAGCT
CCGGCACCATTCTTTATCAGCTTGGGGGCTGGGACGCGCCTTGGGGGATCGAATATCGCATCGATGAGCTCAACGCCCTT
TTACTGCTGATCATCTCAACTATTAGTACCATCGTCCTGTTTGCCGCACAAACCAGTCTTCAAAAAGAGCTGCCTAAAGA
GCGTCACACGCTGTTTTACAGCCTCTACCTGCTGTCATTGACTGGGATGTTCGGCATCGTCGCCACTGGCGATCTGTTTA
ATGTATTCGTATTTCTAGAGATCTCCTCGTTATCAGGCTACGCCCTGATTGCTTTGGGCAAAGATAGACGCGCTCTTTGG
GCCGCCTACCAATATCTGATCATGGGGACTATCGGCGCGACCTTTATTCTGATCGGCATCGGTCTGCTGTATCAGATGAC
AGGCACGCTAAATATGGATGATCTCGCCAACCGGCTACCAGAGGTTGACCAAACACGTACCGTATTTGCCGCGTTTGCCT
TCTTAATCGTTGGGGTCTGTTTAAAGTTGGCACTATTTCCACTGCATCTATGGTTACCTAACGCCTACGCCTATGCTCCT
TCAATAGTGACTGCATTCCTCGCCGCTACGGCAACTAAAGTTGCGGTTTACTTATTGATCCGCTTCACCTATACGATATT
TGGCATCGAGCTTTCCTTCTCCATCCTGCCACTGCAGAACTTATTTATGGTCCTCGGGCTGGTTGGCATATTCGCCGCTT
CTACTGCGGCGATATATAAAACCAATGTTAAACATATCTTTGCCTACTCGAGCATCGCTCAGGTTGGCTACATGATTATT
GGCTACGCCATCAACACCCCCACAGGGCTTATGGCTACTCTGCTGCACCTGTTTAATCACGCCTTAATGAAGAGTGCGCT
GTTTTTAGCGCTAGGCGCGGTGATGTACCGTATCGGCAGCGTACAACTTAGCCAGTTCCAAGGGCTAGGACGACAGATGC
CGCTAACCATGGCCGCCATAGTTGTAGGCGGGTTAAGTTTGATAGGCGTCCCCCTGACAGTCGGTTTTATCAGCAAGTGG
TATCTGCTCATCGCCGCCGTTGAGGCTGGAATGTGGCCAGTGGCGGTGTTGGTGCTACTCGGTTCATTACTGGCTGTGCT
TTATATATGGCGAATCGTTGAAATCGCCTATTTCAAGCCCCCCGTACCGGGTCGTGAACCCGTCAAGGAAGCCCCTTGGA
CCTTCCTTGTCCCGATTTGGGTGCTGGTACTGGCCAATATCTATTTTGGCATCGATACCCGCCTTAGTGTGCAGGTGGCA
CAGGCGGCCTCTCAAAGCTTGTTTGGAATTAACCCATGA

Upstream 100 bases:

>100_bases
CACTAGGGCTCGCCTTGGTGGTAAGAATCAAAGAATCATACGGCTCTATAGAAGAGGATGAGATTCAAATTCAAGATCAG
CTGACTGAGGACAAAACATA

Downstream 100 bases:

>100_bases
ATCACTCTTTGGATCTATTGCTACAGCTAACCATTATATTGCCGCTGCTTGCCACTTTGGCGATCTTGGCAACGGGAAAG
CACCCCAACCTTCGTGAGGC

Product: putative monovalent cation/H+ antiporter subunit D

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MLAHLPILQVIVPLMAAPLCWFLDRSRLVWLFALLVSGFSCLTSIALLQQVMSSGTILYQLGGWDAPWGIEYRIDELNAL
LLLIISTISTIVLFAAQTSLQKELPKERHTLFYSLYLLSLTGMFGIVATGDLFNVFVFLEISSLSGYALIALGKDRRALW
AAYQYLIMGTIGATFILIGIGLLYQMTGTLNMDDLANRLPEVDQTRTVFAAFAFLIVGVCLKLALFPLHLWLPNAYAYAP
SIVTAFLAATATKVAVYLLIRFTYTIFGIELSFSILPLQNLFMVLGLVGIFAASTAAIYKTNVKHIFAYSSIAQVGYMII
GYAINTPTGLMATLLHLFNHALMKSALFLALGAVMYRIGSVQLSQFQGLGRQMPLTMAAIVVGGLSLIGVPLTVGFISKW
YLLIAAVEAGMWPVAVLVLLGSLLAVLYIWRIVEIAYFKPPVPGREPVKEAPWTFLVPIWVLVLANIYFGIDTRLSVQVA
QAASQSLFGINP

Sequences:

>Translated_492_residues
MLAHLPILQVIVPLMAAPLCWFLDRSRLVWLFALLVSGFSCLTSIALLQQVMSSGTILYQLGGWDAPWGIEYRIDELNAL
LLLIISTISTIVLFAAQTSLQKELPKERHTLFYSLYLLSLTGMFGIVATGDLFNVFVFLEISSLSGYALIALGKDRRALW
AAYQYLIMGTIGATFILIGIGLLYQMTGTLNMDDLANRLPEVDQTRTVFAAFAFLIVGVCLKLALFPLHLWLPNAYAYAP
SIVTAFLAATATKVAVYLLIRFTYTIFGIELSFSILPLQNLFMVLGLVGIFAASTAAIYKTNVKHIFAYSSIAQVGYMII
GYAINTPTGLMATLLHLFNHALMKSALFLALGAVMYRIGSVQLSQFQGLGRQMPLTMAAIVVGGLSLIGVPLTVGFISKW
YLLIAAVEAGMWPVAVLVLLGSLLAVLYIWRIVEIAYFKPPVPGREPVKEAPWTFLVPIWVLVLANIYFGIDTRLSVQVA
QAASQSLFGINP
>Mature_492_residues
MLAHLPILQVIVPLMAAPLCWFLDRSRLVWLFALLVSGFSCLTSIALLQQVMSSGTILYQLGGWDAPWGIEYRIDELNAL
LLLIISTISTIVLFAAQTSLQKELPKERHTLFYSLYLLSLTGMFGIVATGDLFNVFVFLEISSLSGYALIALGKDRRALW
AAYQYLIMGTIGATFILIGIGLLYQMTGTLNMDDLANRLPEVDQTRTVFAAFAFLIVGVCLKLALFPLHLWLPNAYAYAP
SIVTAFLAATATKVAVYLLIRFTYTIFGIELSFSILPLQNLFMVLGLVGIFAASTAAIYKTNVKHIFAYSSIAQVGYMII
GYAINTPTGLMATLLHLFNHALMKSALFLALGAVMYRIGSVQLSQFQGLGRQMPLTMAAIVVGGLSLIGVPLTVGFISKW
YLLIAAVEAGMWPVAVLVLLGSLLAVLYIWRIVEIAYFKPPVPGREPVKEAPWTFLVPIWVLVLANIYFGIDTRLSVQVA
QAASQSLFGINP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=366, Percent_Identity=26.2295081967213, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI251831116, Length=372, Percent_Identity=24.7311827956989, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788831, Length=351, Percent_Identity=29.3447293447293, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI145693160, Length=357, Percent_Identity=29.1316526610644, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788827, Length=367, Percent_Identity=26.7029972752044, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1788614, Length=408, Percent_Identity=27.6960784313726, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788829, Length=405, Percent_Identity=27.1604938271605, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788613, Length=457, Percent_Identity=26.0393873085339, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI2367154, Length=367, Percent_Identity=25.3405994550409, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53944; Mature: 53944

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAHLPILQVIVPLMAAPLCWFLDRSRLVWLFALLVSGFSCLTSIALLQQVMSSGTILYQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
LGGWDAPWGIEYRIDELNALLLLIISTISTIVLFAAQTSLQKELPKERHTLFYSLYLLSL
ECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TGMFGIVATGDLFNVFVFLEISSLSGYALIALGKDRRALWAAYQYLIMGTIGATFILIGI
HHHHHHHCCCHHHEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
GLLYQMTGTLNMDDLANRLPEVDQTRTVFAAFAFLIVGVCLKLALFPLHLWLPNAYAYAP
HHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
SIVTAFLAATATKVAVYLLIRFTYTIFGIELSFSILPLQNLFMVLGLVGIFAASTAAIYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
TNVKHIFAYSSIAQVGYMIIGYAINTPTGLMATLLHLFNHALMKSALFLALGAVMYRIGS
CCHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VQLSQFQGLGRQMPLTMAAIVVGGLSLIGVPLTVGFISKWYLLIAAVEAGMWPVAVLVLL
CHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
GSLLAVLYIWRIVEIAYFKPPVPGREPVKEAPWTFLVPIWVLVLANIYFGIDTRLSVQVA
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
QAASQSLFGINP
HHHHHCCCCCCC
>Mature Secondary Structure
MLAHLPILQVIVPLMAAPLCWFLDRSRLVWLFALLVSGFSCLTSIALLQQVMSSGTILYQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
LGGWDAPWGIEYRIDELNALLLLIISTISTIVLFAAQTSLQKELPKERHTLFYSLYLLSL
ECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TGMFGIVATGDLFNVFVFLEISSLSGYALIALGKDRRALWAAYQYLIMGTIGATFILIGI
HHHHHHHCCCHHHEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
GLLYQMTGTLNMDDLANRLPEVDQTRTVFAAFAFLIVGVCLKLALFPLHLWLPNAYAYAP
HHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
SIVTAFLAATATKVAVYLLIRFTYTIFGIELSFSILPLQNLFMVLGLVGIFAASTAAIYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
TNVKHIFAYSSIAQVGYMIIGYAINTPTGLMATLLHLFNHALMKSALFLALGAVMYRIGS
CCHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VQLSQFQGLGRQMPLTMAAIVVGGLSLIGVPLTVGFISKWYLLIAAVEAGMWPVAVLVLL
CHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
GSLLAVLYIWRIVEIAYFKPPVPGREPVKEAPWTFLVPIWVLVLANIYFGIDTRLSVQVA
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
QAASQSLFGINP
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA