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The map label for this gene is nuoN [H]

Identifier: 157963101

GI number: 157963101

Start: 4026289

End: 4027779

Strand: Direct

Name: nuoN [H]

Synonym: Spea_3285

Alternate gene names: 157963101

Gene position: 4026289-4027779 (Clockwise)

Preceding gene: 157963100

Following gene: 157963102

Centisome position: 77.81

GC content: 50.7

Gene sequence:

>1491_bases
ATGAATCACTCTTTGGATCTATTGCTACAGCTAACCATTATATTGCCGCTGCTTGCCACTTTGGCGATCTTGGCAACGGG
AAAGCACCCCAACCTTCGTGAGGCTGTAACGATAAGCATCAGCCTTGCCGTTATGTACTGTGTGATAAACCTATATCAAG
GGCTCAGTGCAGGCGCATCTATCGAGGTGTTTTGGTGGCAACTGCTACCGGGCTTGGAAGTGAGCTTTGTGGTTGAGCCT
TTAGGTATGCTGTTTGCCCTCATTGCCAGCTTTCTGTGGCTCATTACCACCCTATACGCCATCGGCTATATGCGCGGCCA
TGGGGAGGAGAATCAAACCCGCTTTTACCTCTGCTTTGCGTTGGCCATAAGCGCAGTGATGGGATTAGCATTTTCCGCCA
ACCTGTTTACCCTGTTTATCTTCTACGAAGTCTTGACCCTGTCCACCTATCCTTTAGTGACCCACGCTGGCACAGAAAAA
GCCAAGCAAGGGGGGCGCGTATACTTAGGGATCTTACTCGGGACCTCCATCGCCTTCTTCCTTTTAGCGATTATCTTGAC
CTGGTTTGTGGCCGGCACCTTAGACTTCAAACCCGGCGGCGTGTTTCACACTGATGTTGATACCAGCTTACTGGGAGCCA
TACTGGTACTGTTTGTGTTTGGTATTGGTAAGGCGGCCATCATGCCGTTCCATCGTTGGCTCCCCGCCGCTATGGTGGCG
CCAACACCGGTGAGTGCCCTGCTGCATGCGGTAGCTGTGGTCAAGGCTGGGGTGTTCACCATCTTAAAGATCTGCGTGTT
TATCTTTGGTATCGAGCTACTACCTACTCTGCCTACGACCGAATTCCTGCTCTATTTAGCTGGCGCATCGGTATTGCTCG
CCTCCATAGTGGCAATGCGTCAGGATAACTTAAAAGCACGACTCGCCTACTCAACGGTGAGTCAACTGGGCTATATCACC
ATAGGCGCACTGCTGGCCACCTCATCAGGGGTCATTGGTAGCTCGATGCATATTGCAACCCACGCTTTTGGCAAGATCAC
CCTATTCTTCTGTGCAGGCGCGATTCTGGTTGCGACCCATAAATCAAAAGTAAGTGAAATGCGCGGCCTCGGCTTTACCA
TGCCTGTCACCATGGCGGCTTTTTTTATCGCCAGTCTAAGTATTATTGGTGTTCCACCCGCAGGCGGTACGTGGAGTAAG
TGGTTCCTGTTAATGGGCACAATGGAAACCGAACATTGGGTCATTATGATGGTGCTGATGGTGAGCTCTCTGCTCAATAT
CGCCTATCTTCTACCTATTCCATACCATGCCTTTTTCCCAGGCAAGGGCCATGCCGCAACTAAGACTGGGATCAAGGAAG
CCCCTCTCATGTCATTAATCGCGCTGTCCATCACCACCCTAGGTTGCTTGATTCTATTCATCTATCCGCAACCCCTCTAT
GAGTTGGCAAAAACCATCTTAACTGTACCGGGAGTCAGCCATGGACAATAA

Upstream 100 bases:

>100_bases
TTTGGGTGCTGGTACTGGCCAATATCTATTTTGGCATCGATACCCGCCTTAGTGTGCAGGTGGCACAGGCGGCCTCTCAA
AGCTTGTTTGGAATTAACCC

Downstream 100 bases:

>100_bases
CAGTAACAAGCAATATCTGTTTGATAATCCTAAGAACATAAAACGTGTTCTGTATCTTTTATATGCCTGCTGCACCCTGC
TCGTGGTGCTTGATTTTGTT

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MNHSLDLLLQLTIILPLLATLAILATGKHPNLREAVTISISLAVMYCVINLYQGLSAGASIEVFWWQLLPGLEVSFVVEP
LGMLFALIASFLWLITTLYAIGYMRGHGEENQTRFYLCFALAISAVMGLAFSANLFTLFIFYEVLTLSTYPLVTHAGTEK
AKQGGRVYLGILLGTSIAFFLLAIILTWFVAGTLDFKPGGVFHTDVDTSLLGAILVLFVFGIGKAAIMPFHRWLPAAMVA
PTPVSALLHAVAVVKAGVFTILKICVFIFGIELLPTLPTTEFLLYLAGASVLLASIVAMRQDNLKARLAYSTVSQLGYIT
IGALLATSSGVIGSSMHIATHAFGKITLFFCAGAILVATHKSKVSEMRGLGFTMPVTMAAFFIASLSIIGVPPAGGTWSK
WFLLMGTMETEHWVIMMVLMVSSLLNIAYLLPIPYHAFFPGKGHAATKTGIKEAPLMSLIALSITTLGCLILFIYPQPLY
ELAKTILTVPGVSHGQ

Sequences:

>Translated_496_residues
MNHSLDLLLQLTIILPLLATLAILATGKHPNLREAVTISISLAVMYCVINLYQGLSAGASIEVFWWQLLPGLEVSFVVEP
LGMLFALIASFLWLITTLYAIGYMRGHGEENQTRFYLCFALAISAVMGLAFSANLFTLFIFYEVLTLSTYPLVTHAGTEK
AKQGGRVYLGILLGTSIAFFLLAIILTWFVAGTLDFKPGGVFHTDVDTSLLGAILVLFVFGIGKAAIMPFHRWLPAAMVA
PTPVSALLHAVAVVKAGVFTILKICVFIFGIELLPTLPTTEFLLYLAGASVLLASIVAMRQDNLKARLAYSTVSQLGYIT
IGALLATSSGVIGSSMHIATHAFGKITLFFCAGAILVATHKSKVSEMRGLGFTMPVTMAAFFIASLSIIGVPPAGGTWSK
WFLLMGTMETEHWVIMMVLMVSSLLNIAYLLPIPYHAFFPGKGHAATKTGIKEAPLMSLIALSITTLGCLILFIYPQPLY
ELAKTILTVPGVSHGQ
>Mature_496_residues
MNHSLDLLLQLTIILPLLATLAILATGKHPNLREAVTISISLAVMYCVINLYQGLSAGASIEVFWWQLLPGLEVSFVVEP
LGMLFALIASFLWLITTLYAIGYMRGHGEENQTRFYLCFALAISAVMGLAFSANLFTLFIFYEVLTLSTYPLVTHAGTEK
AKQGGRVYLGILLGTSIAFFLLAIILTWFVAGTLDFKPGGVFHTDVDTSLLGAILVLFVFGIGKAAIMPFHRWLPAAMVA
PTPVSALLHAVAVVKAGVFTILKICVFIFGIELLPTLPTTEFLLYLAGASVLLASIVAMRQDNLKARLAYSTVSQLGYIT
IGALLATSSGVIGSSMHIATHAFGKITLFFCAGAILVATHKSKVSEMRGLGFTMPVTMAAFFIASLSIIGVPPAGGTWSK
WFLLMGTMETEHWVIMMVLMVSSLLNIAYLLPIPYHAFFPGKGHAATKTGIKEAPLMSLIALSITTLGCLILFIYPQPLY
ELAKTILTVPGVSHGQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=360, Percent_Identity=31.1111111111111, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1788829, Length=401, Percent_Identity=29.426433915212, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1788614, Length=483, Percent_Identity=28.3643892339545, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788827, Length=320, Percent_Identity=30.9375, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI145693160, Length=429, Percent_Identity=25.6410256410256, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788831, Length=332, Percent_Identity=28.3132530120482, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788613, Length=393, Percent_Identity=22.9007633587786, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI2367154, Length=346, Percent_Identity=26.5895953757225, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53445; Mature: 53445

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSLDLLLQLTIILPLLATLAILATGKHPNLREAVTISISLAVMYCVINLYQGLSAGAS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEHHHHHHHHHHHHHHHCCCCCCE
IEVFWWQLLPGLEVSFVVEPLGMLFALIASFLWLITTLYAIGYMRGHGEENQTRFYLCFA
EEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LAISAVMGLAFSANLFTLFIFYEVLTLSTYPLVTHAGTEKAKQGGRVYLGILLGTSIAFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCEEEHHHHHHHHHHH
LLAIILTWFVAGTLDFKPGGVFHTDVDTSLLGAILVLFVFGIGKAAIMPFHRWLPAAMVA
HHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCC
PTPVSALLHAVAVVKAGVFTILKICVFIFGIELLPTLPTTEFLLYLAGASVLLASIVAMR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
QDNLKARLAYSTVSQLGYITIGALLATSSGVIGSSMHIATHAFGKITLFFCAGAILVATH
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEC
KSKVSEMRGLGFTMPVTMAAFFIASLSIIGVPPAGGTWSKWFLLMGTMETEHWVIMMVLM
HHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHEEECCCCCHHHHHHHHH
VSSLLNIAYLLPIPYHAFFPGKGHAATKTGIKEAPLMSLIALSITTLGCLILFIYPQPLY
HHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
ELAKTILTVPGVSHGQ
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNHSLDLLLQLTIILPLLATLAILATGKHPNLREAVTISISLAVMYCVINLYQGLSAGAS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEHHHHHHHHHHHHHHHCCCCCCE
IEVFWWQLLPGLEVSFVVEPLGMLFALIASFLWLITTLYAIGYMRGHGEENQTRFYLCFA
EEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LAISAVMGLAFSANLFTLFIFYEVLTLSTYPLVTHAGTEKAKQGGRVYLGILLGTSIAFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCEEEHHHHHHHHHHH
LLAIILTWFVAGTLDFKPGGVFHTDVDTSLLGAILVLFVFGIGKAAIMPFHRWLPAAMVA
HHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCC
PTPVSALLHAVAVVKAGVFTILKICVFIFGIELLPTLPTTEFLLYLAGASVLLASIVAMR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
QDNLKARLAYSTVSQLGYITIGALLATSSGVIGSSMHIATHAFGKITLFFCAGAILVATH
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEC
KSKVSEMRGLGFTMPVTMAAFFIASLSIIGVPPAGGTWSKWFLLMGTMETEHWVIMMVLM
HHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHEEECCCCCHHHHHHHHH
VSSLLNIAYLLPIPYHAFFPGKGHAATKTGIKEAPLMSLIALSITTLGCLILFIYPQPLY
HHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
ELAKTILTVPGVSHGQ
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA