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The map label for this gene is guaD [H]

Identifier: 157963060

GI number: 157963060

Start: 3977193

End: 3978557

Strand: Direct

Name: guaD [H]

Synonym: Spea_3243

Alternate gene names: 157963060

Gene position: 3977193-3978557 (Clockwise)

Preceding gene: 157963057

Following gene: 157963062

Centisome position: 76.86

GC content: 47.33

Gene sequence:

>1365_bases
ATGTCAGAGATTAAATTTGGTGTATTAGGACAGTATTTTCATTGTCCAGTGAAAGGCGATTTTGAGTATGCGGCTCAAGC
TTTGGTGCTCGTTGATAATAGCGGCGCTATTTCATCAATTATCAACGAAAATGATGCCGATTTTCAAACACAGATCAGCG
AGTTATCCGCTCGCAAACAACTCATCGAATTAAGCCCGACTCAGTATCTAATGCCAGGCATGGTTGACTTGCACGTACAT
GCGCCGCAATTTCCTCAAGCCGGAAAAGGGCTAGATCTGCCTCTATACGATTGGCTACAAGACTATACTTTTCCGCTAGA
AGCTAAATTTGAGGATATGGATTTTGCCAAGAAAATTTACCCAGAGTTAGTCCAGTCACTGCTCGCTAATGGCACCACCA
GCGCAGTGTATTTTGCCACTATCCATAAAGACACGAGCGTTGAGCTTGCCCGTGAGTGTGTAAAGCAAGGTCAGCGCGGC
TTTATCGGCAAGGTCAATATGGATGAGGCGAGTCAATGCCCAGCATTTTACATTGAGCCATCTACAAGCGACGCCCTAAT
CGACACAGAAAACTTTATTCAGCAAGTACAGGCACTCGAAGGCAACGAGCACAGATTAGTGTCGCCAGTAGTCACTCCGC
GCTTTGTGCCAAGTTGCAGCAGCGAGATGCTGCAAGGCTTAGGCGAACTGGTACAAAAGTACCAGTGCCATGTGCAGACC
CACTGCTCTGAAAGCGACTGGGCAAGGGATTACAGCCAAGAGAAGTACGGTAAAACAGACGTTGAGATCTACAGCGATTT
TGGCTTAATGACCAACAAAACCATTTTGGCACACTCGATATTCCTAACGCCGAATGATCACAAGGTGATTAAGGCGACAG
GCGCCAGTATTGCCCACTGCCCGCTATCTAACATGTATTTTGCCAATGCGGCGATGCAGACCCGTGAGATCTTGGATAAC
GATCTAAAATGTGGTTTAGGTAGTGATTTAGCAGGCGCGCCAATTCCATCGATTTTCCATACTTGTTTAGATGCGGTAAA
CCACTCGCGAGTTCGCGAAGATGGCACATCCACTTATCTGCCTGCTGATACTCGTGGTGAAAGCGGATCGCGCATCTCAT
TCCTAGAGTCTTACTGGATGGCGACAGTTGGTGGCGGACAGGCAATAGATGCCAAAGTCGGTTTGTTCCAAGCAGGCTTC
GAGTTCGACGCCTTAGTGATTGATACCGCCGTTAGCGACAGCAATATCTTTATCCTTGATGAGATGGACAGCGCTAAAGA
CAAGCTTGAGAAAATCATCACCCTCAATAGCCGCCAAAACATCCGCAAGGTGTGGGTCAAAGGTCAGTTGGTGGTAGAGA
AGTAA

Upstream 100 bases:

>100_bases
AGCTTGGTCACAAATTGCAGCTTATTTTATTAAATTTGATTGAGCTCATGTAATACTCCGCCAGCGAAAACCCTACAATT
AATAAGATGGATGCATACTG

Downstream 100 bases:

>100_bases
GCCACTTACTTTCTAAACTTAGTTGACTAGTCATATAAAAAAGGCTCAGAAGATCATCCTTCTGAGCCTTTTATTTGTTT
ATCACTTTTACAGACTACTC

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSEIKFGVLGQYFHCPVKGDFEYAAQALVLVDNSGAISSIINENDADFQTQISELSARKQLIELSPTQYLMPGMVDLHVH
APQFPQAGKGLDLPLYDWLQDYTFPLEAKFEDMDFAKKIYPELVQSLLANGTTSAVYFATIHKDTSVELARECVKQGQRG
FIGKVNMDEASQCPAFYIEPSTSDALIDTENFIQQVQALEGNEHRLVSPVVTPRFVPSCSSEMLQGLGELVQKYQCHVQT
HCSESDWARDYSQEKYGKTDVEIYSDFGLMTNKTILAHSIFLTPNDHKVIKATGASIAHCPLSNMYFANAAMQTREILDN
DLKCGLGSDLAGAPIPSIFHTCLDAVNHSRVREDGTSTYLPADTRGESGSRISFLESYWMATVGGGQAIDAKVGLFQAGF
EFDALVIDTAVSDSNIFILDEMDSAKDKLEKIITLNSRQNIRKVWVKGQLVVEK

Sequences:

>Translated_454_residues
MSEIKFGVLGQYFHCPVKGDFEYAAQALVLVDNSGAISSIINENDADFQTQISELSARKQLIELSPTQYLMPGMVDLHVH
APQFPQAGKGLDLPLYDWLQDYTFPLEAKFEDMDFAKKIYPELVQSLLANGTTSAVYFATIHKDTSVELARECVKQGQRG
FIGKVNMDEASQCPAFYIEPSTSDALIDTENFIQQVQALEGNEHRLVSPVVTPRFVPSCSSEMLQGLGELVQKYQCHVQT
HCSESDWARDYSQEKYGKTDVEIYSDFGLMTNKTILAHSIFLTPNDHKVIKATGASIAHCPLSNMYFANAAMQTREILDN
DLKCGLGSDLAGAPIPSIFHTCLDAVNHSRVREDGTSTYLPADTRGESGSRISFLESYWMATVGGGQAIDAKVGLFQAGF
EFDALVIDTAVSDSNIFILDEMDSAKDKLEKIITLNSRQNIRKVWVKGQLVVEK
>Mature_453_residues
SEIKFGVLGQYFHCPVKGDFEYAAQALVLVDNSGAISSIINENDADFQTQISELSARKQLIELSPTQYLMPGMVDLHVHA
PQFPQAGKGLDLPLYDWLQDYTFPLEAKFEDMDFAKKIYPELVQSLLANGTTSAVYFATIHKDTSVELARECVKQGQRGF
IGKVNMDEASQCPAFYIEPSTSDALIDTENFIQQVQALEGNEHRLVSPVVTPRFVPSCSSEMLQGLGELVQKYQCHVQTH
CSESDWARDYSQEKYGKTDVEIYSDFGLMTNKTILAHSIFLTPNDHKVIKATGASIAHCPLSNMYFANAAMQTREILDND
LKCGLGSDLAGAPIPSIFHTCLDAVNHSRVREDGTSTYLPADTRGESGSRISFLESYWMATVGGGQAIDAKVGLFQAGFE
FDALVIDTAVSDSNIFILDEMDSAKDKLEKIITLNSRQNIRKVWVKGQLVVEK

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=452, Percent_Identity=35.8407079646018, Blast_Score=263, Evalue=3e-70,
Organism=Escherichia coli, GI1789249, Length=396, Percent_Identity=28.5353535353535, Blast_Score=159, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6319963, Length=416, Percent_Identity=34.8557692307692, Blast_Score=219, Evalue=5e-58,
Organism=Drosophila melanogaster, GI24643849, Length=456, Percent_Identity=34.8684210526316, Blast_Score=269, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 50290; Mature: 50159

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIKFGVLGQYFHCPVKGDFEYAAQALVLVDNSGAISSIINENDADFQTQISELSARKQ
CCCCCCCCCCCEEECCCCCCHHHCCEEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHH
LIELSPTQYLMPGMVDLHVHAPQFPQAGKGLDLPLYDWLQDYTFPLEAKFEDMDFAKKIY
HHHCCCCHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHH
PELVQSLLANGTTSAVYFATIHKDTSVELARECVKQGQRGFIGKVNMDEASQCPAFYIEP
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCEEECCCCCHHCCCEEEECC
STSDALIDTENFIQQVQALEGNEHRLVSPVVTPRFVPSCSSEMLQGLGELVQKYQCHVQT
CCCCCEECHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HCSESDWARDYSQEKYGKTDVEIYSDFGLMTNKTILAHSIFLTPNDHKVIKATGASIAHC
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCEEEEECCCCEEEC
PLSNMYFANAAMQTREILDNDLKCGLGSDLAGAPIPSIFHTCLDAVNHSRVREDGTSTYL
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
PADTRGESGSRISFLESYWMATVGGGQAIDAKVGLFQAGFEFDALVIDTAVSDSNIFILD
CCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCHHHEECCCCCCEEEEEEEECCCCEEEEE
EMDSAKDKLEKIITLNSRQNIRKVWVKGQLVVEK
CCCHHHHHHHHHHCCCCCCCHHEEEEECEEEEEC
>Mature Secondary Structure 
SEIKFGVLGQYFHCPVKGDFEYAAQALVLVDNSGAISSIINENDADFQTQISELSARKQ
CCCCCCCCCCEEECCCCCCHHHCCEEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHH
LIELSPTQYLMPGMVDLHVHAPQFPQAGKGLDLPLYDWLQDYTFPLEAKFEDMDFAKKIY
HHHCCCCHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHH
PELVQSLLANGTTSAVYFATIHKDTSVELARECVKQGQRGFIGKVNMDEASQCPAFYIEP
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCEEECCCCCHHCCCEEEECC
STSDALIDTENFIQQVQALEGNEHRLVSPVVTPRFVPSCSSEMLQGLGELVQKYQCHVQT
CCCCCEECHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HCSESDWARDYSQEKYGKTDVEIYSDFGLMTNKTILAHSIFLTPNDHKVIKATGASIAHC
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCEEEEECCCCEEEC
PLSNMYFANAAMQTREILDNDLKCGLGSDLAGAPIPSIFHTCLDAVNHSRVREDGTSTYL
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
PADTRGESGSRISFLESYWMATVGGGQAIDAKVGLFQAGFEFDALVIDTAVSDSNIFILD
CCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCHHHEECCCCCCEEEEEEEECCCCEEEEE
EMDSAKDKLEKIITLNSRQNIRKVWVKGQLVVEK
CCCHHHHHHHHHHCCCCCCCHHEEEEECEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]