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The map label for this gene is dacA [H]

Identifier: 157962970

GI number: 157962970

Start: 3858541

End: 3859713

Strand: Direct

Name: dacA [H]

Synonym: Spea_3153

Alternate gene names: 157962970

Gene position: 3858541-3859713 (Clockwise)

Preceding gene: 157962969

Following gene: 157962971

Centisome position: 74.57

GC content: 44.16

Gene sequence:

>1173_bases
ATGAATTTATTGAACAAACCTTTAAAAACACTTTTGTTCGCGACTTCAGTCGCCGCCTTATCGGCACATGCGGCCCCCGT
CGTTACCCCAAATGCACCAACAGTTGCGGCCAAAGCCTATGTGCTGATGGATTACAACACCGGACAGATCATCGCTGAAG
AAAACGCTTACGAAAGCTTAAACCCAGCCAGCTTAACTAAGATGATGACCAGCTATGTTATCGGTCACGAAATTAAAGTC
GGCAATGTTTCACCAAGTGACGAAGTTACAATTAGCAAAAAAGCCTGGTCTAAAAATTTCCCTGATTCGTCTAAGATGTT
TATCGAAGTGGGTAAAAAAGTCACTGTAGAAGATCTAAACAAGGGCATTATCATTCAATCGGGTAACGATGCCTGTGTTG
CTATGGCTGAACACATTGCAGGCACAGAAGATGGCTTCGTTGACCTAATGAACTCTTGGGCTAAGCAGCTAGGTATGCAA
GATAGCTACTTTGAAAACAGCCACGGTCTAGACTCTGACAATCACAAGAGTACTGCCTATGATATGGCAATTCTAGGCGC
TGCGATTATTCGTGATGTACCAGAAGAGTACGCTGTTTACAAAGAGAAATCATTTACCTACAACGGGATCAAGCAGTACA
ACCGTAATGGCTTATTATGGGATAAGAGCCTGAGCGTTGACGGCATCAAAACCGGTCACACTTCAGGTGCAGGCTTTAAC
TTAGTAACCTCTGCGACCAAAGACGGTATGCGTCTAATCTCTGTTGTGATGGGTACCAAGAGTGAAGCGGCGCGTAAGGC
TGAAAGCAAGAAACTACTTAACTACGGCTTCCGTTTCTTTGAAACTGTTACACCATACAAAGCTGGCGACACATTTGTCA
CTCAAAAAATCTGGTACGGCGATCGTGAGACGATTGACTTAGGTGTGGTAACTGACACCCCGATTACCATCAGTCGCGGT
CAAGCTAAGAACCTACAGGCTAACTTTGAGCTAACTAAAGAGCTTAATGCACCAATTGCTAAAGGTGAAACTGTTGGTCG
TGTTTACTTCCAACTAAACGGAAAAGATATCGCTCAGTTCCCACTTGTAACCCTACAAGAAGTAAACGAAGGCAGCTGGT
TCAGCCAGTTGATGGATTACTTTAAGCAGATGTTTGCAGGTTGGTTTAGCTAA

Upstream 100 bases:

>100_bases
GATTAATGAACCAACGCTGAATTCCCTTGTCGACTAATGTTATACTCGTGGGAATTACACTTTCTTCATACATCAACTTA
TTAAGACGTGCATTATTAAT

Downstream 100 bases:

>100_bases
GCCATACTCGGTATATCAAGAAGCCACCCTAGGGTGGCTTCTTGCGTTTTATACTTCAAACAAGCCTATACCACTTGAAT
AAGGTATAATTGCCACCATA

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MNLLNKPLKTLLFATSVAALSAHAAPVVTPNAPTVAAKAYVLMDYNTGQIIAEENAYESLNPASLTKMMTSYVIGHEIKV
GNVSPSDEVTISKKAWSKNFPDSSKMFIEVGKKVTVEDLNKGIIIQSGNDACVAMAEHIAGTEDGFVDLMNSWAKQLGMQ
DSYFENSHGLDSDNHKSTAYDMAILGAAIIRDVPEEYAVYKEKSFTYNGIKQYNRNGLLWDKSLSVDGIKTGHTSGAGFN
LVTSATKDGMRLISVVMGTKSEAARKAESKKLLNYGFRFFETVTPYKAGDTFVTQKIWYGDRETIDLGVVTDTPITISRG
QAKNLQANFELTKELNAPIAKGETVGRVYFQLNGKDIAQFPLVTLQEVNEGSWFSQLMDYFKQMFAGWFS

Sequences:

>Translated_390_residues
MNLLNKPLKTLLFATSVAALSAHAAPVVTPNAPTVAAKAYVLMDYNTGQIIAEENAYESLNPASLTKMMTSYVIGHEIKV
GNVSPSDEVTISKKAWSKNFPDSSKMFIEVGKKVTVEDLNKGIIIQSGNDACVAMAEHIAGTEDGFVDLMNSWAKQLGMQ
DSYFENSHGLDSDNHKSTAYDMAILGAAIIRDVPEEYAVYKEKSFTYNGIKQYNRNGLLWDKSLSVDGIKTGHTSGAGFN
LVTSATKDGMRLISVVMGTKSEAARKAESKKLLNYGFRFFETVTPYKAGDTFVTQKIWYGDRETIDLGVVTDTPITISRG
QAKNLQANFELTKELNAPIAKGETVGRVYFQLNGKDIAQFPLVTLQEVNEGSWFSQLMDYFKQMFAGWFS
>Mature_390_residues
MNLLNKPLKTLLFATSVAALSAHAAPVVTPNAPTVAAKAYVLMDYNTGQIIAEENAYESLNPASLTKMMTSYVIGHEIKV
GNVSPSDEVTISKKAWSKNFPDSSKMFIEVGKKVTVEDLNKGIIIQSGNDACVAMAEHIAGTEDGFVDLMNSWAKQLGMQ
DSYFENSHGLDSDNHKSTAYDMAILGAAIIRDVPEEYAVYKEKSFTYNGIKQYNRNGLLWDKSLSVDGIKTGHTSGAGFN
LVTSATKDGMRLISVVMGTKSEAARKAESKKLLNYGFRFFETVTPYKAGDTFVTQKIWYGDRETIDLGVVTDTPITISRG
QAKNLQANFELTKELNAPIAKGETVGRVYFQLNGKDIAQFPLVTLQEVNEGSWFSQLMDYFKQMFAGWFS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=395, Percent_Identity=51.6455696202532, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1787062, Length=370, Percent_Identity=53.5135135135135, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI87082022, Length=359, Percent_Identity=47.9108635097493, Blast_Score=357, Evalue=1e-99,
Organism=Escherichia coli, GI87082054, Length=252, Percent_Identity=26.5873015873016, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42916; Mature: 42916

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLNKPLKTLLFATSVAALSAHAAPVVTPNAPTVAAKAYVLMDYNTGQIIAEENAYESL
CCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEEEEECCCCCEEECCCCCCCC
NPASLTKMMTSYVIGHEIKVGNVSPSDEVTISKKAWSKNFPDSSKMFIEVGKKVTVEDLN
CHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEHHHCCCCCCCCCHHHHHCCCEEEEECCC
KGIIIQSGNDACVAMAEHIAGTEDGFVDLMNSWAKQLGMQDSYFENSHGLDSDNHKSTAY
CCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
DMAILGAAIIRDVPEEYAVYKEKSFTYNGIKQYNRNGLLWDKSLSVDGIKTGHTSGAGFN
HHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCE
LVTSATKDGMRLISVVMGTKSEAARKAESKKLLNYGFRFFETVTPYKAGDTFVTQKIWYG
EEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECC
DRETIDLGVVTDTPITISRGQAKNLQANFELTKELNAPIAKGETVGRVYFQLNGKDIAQF
CCCEEEEEEEECCCEEEECCCCCCCEECEEEHHHCCCCCCCCCCEEEEEEEECCCCCCCC
PLVTLQEVNEGSWFSQLMDYFKQMFAGWFS
CCHHHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLLNKPLKTLLFATSVAALSAHAAPVVTPNAPTVAAKAYVLMDYNTGQIIAEENAYESL
CCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEEEEECCCCCEEECCCCCCCC
NPASLTKMMTSYVIGHEIKVGNVSPSDEVTISKKAWSKNFPDSSKMFIEVGKKVTVEDLN
CHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEHHHCCCCCCCCCHHHHHCCCEEEEECCC
KGIIIQSGNDACVAMAEHIAGTEDGFVDLMNSWAKQLGMQDSYFENSHGLDSDNHKSTAY
CCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
DMAILGAAIIRDVPEEYAVYKEKSFTYNGIKQYNRNGLLWDKSLSVDGIKTGHTSGAGFN
HHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCE
LVTSATKDGMRLISVVMGTKSEAARKAESKKLLNYGFRFFETVTPYKAGDTFVTQKIWYG
EEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECC
DRETIDLGVVTDTPITISRGQAKNLQANFELTKELNAPIAKGETVGRVYFQLNGKDIAQF
CCCEEEEEEEECCCEEEECCCCCCCEECEEEHHHCCCCCCCCCCEEEEEEEECCCCCCCC
PLVTLQEVNEGSWFSQLMDYFKQMFAGWFS
CCHHHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]