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The map label for this gene is holA [H]

Identifier: 157962962

GI number: 157962962

Start: 3850943

End: 3851974

Strand: Direct

Name: holA [H]

Synonym: Spea_3145

Alternate gene names: 157962962

Gene position: 3850943-3851974 (Clockwise)

Preceding gene: 157962961

Following gene: 157962963

Centisome position: 74.42

GC content: 47.19

Gene sequence:

>1032_bases
ATGCGGATCTACCCAGATCAGTTAGCGCGTCAGCTTTCTCCACTGCCTCAATGTTGCCTTATTTTTGGTGATGATCCTTG
GTTGTGTGAGCAGAGCCGCGCAGCCCTTCACTCTGAAGCCAAGCGCCAAGGTTTCGAAGAAAAAATCCAACTGAGCCAAG
AGACGGGTTTTAGCTGGAATGAATTGTTCGAACAGTGGCAAGCGATGAGCTTGTTTTCGAGCCGTCGTATCATTGAGTTA
ACTCTTCCCCAAGCAAAACCTGGCACCGACGGCAGCGCCATGCTGCAAACGTTAATGCAGATGGATAATCCAGATGTGTT
ACTGATCTTAACCGGCCCAAAACTTGCGGCAGAGCAAACCAAGAGTAAATGGTTTAAGAGTCTAGATGCTAAAGGCATAT
ATGTGCCTTGCGCGACCCCAGAAGGCGCACAATTTCAGCGTTGGTTAGACGGCCGTATCAATTACTATGGCTTATCGCTA
GCAAGGGATCCTCGTGAGATGCTGTTTGCACTTTACGAAGGTAACCTGCTCGCCGCCGATCAAGCACTGCAGTTACTGCA
ACTATTGAGCCCTACAGCGCCCATTGATTGCGAGCAATTAAGCCAATACTTTGAAGATCAATCGCGCTTTAGTGTGTTTC
AACTCACCGATGCGATGCTCAACAACCAACAAGATAAAGCTCAGCACATTCTATCGCAGTTAAAAAGCGAAGGGGTTGCC
ATGCCAATTATCCTCTGGTCGTTATTTAAAGAGCTTGCGGTGCTGCTACAACTGAAAACAGCACAAGACAAGCGTGAGTC
TTTACAAGGGCTTTGGTGCAAGTTACGTATTTGGGATAAACGTAAACCTATCTACCAGAATGCCTTGAACCGTCTAGAGC
TCACTCATATTGAAACCTTACTTGCCGGAGCCTCTGCTATCGAGCTAAAGCTAAAGCAGCAAGGAATAGAAGATTGGACA
GGGCTAAGCCATATCAGCTTATTGTTCGATGCCAAGGCCCATAACAAGCTGGCACATATCGCGTTAGACTAA

Upstream 100 bases:

>100_bases
CATTAGCAAAAAGCCGAGAGATGGAGCTATTGCTAAAAGAGATGCGCATTCAAGCAGCCGACCGCATTATTCAAACTTTG
GCCGCGACTGAGGTTAATTG

Downstream 100 bases:

>100_bases
TGAAAATAGGCATTTTAGGTGGCACATTCGACCCGATCCACTTTGGCCATATCCGTCCGGCCCAAGAGGTGAAGCAGCAG
CTTAAGTTAGATGAGGTTTG

Product: DNA polymerase III subunit delta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MRIYPDQLARQLSPLPQCCLIFGDDPWLCEQSRAALHSEAKRQGFEEKIQLSQETGFSWNELFEQWQAMSLFSSRRIIEL
TLPQAKPGTDGSAMLQTLMQMDNPDVLLILTGPKLAAEQTKSKWFKSLDAKGIYVPCATPEGAQFQRWLDGRINYYGLSL
ARDPREMLFALYEGNLLAADQALQLLQLLSPTAPIDCEQLSQYFEDQSRFSVFQLTDAMLNNQQDKAQHILSQLKSEGVA
MPIILWSLFKELAVLLQLKTAQDKRESLQGLWCKLRIWDKRKPIYQNALNRLELTHIETLLAGASAIELKLKQQGIEDWT
GLSHISLLFDAKAHNKLAHIALD

Sequences:

>Translated_343_residues
MRIYPDQLARQLSPLPQCCLIFGDDPWLCEQSRAALHSEAKRQGFEEKIQLSQETGFSWNELFEQWQAMSLFSSRRIIEL
TLPQAKPGTDGSAMLQTLMQMDNPDVLLILTGPKLAAEQTKSKWFKSLDAKGIYVPCATPEGAQFQRWLDGRINYYGLSL
ARDPREMLFALYEGNLLAADQALQLLQLLSPTAPIDCEQLSQYFEDQSRFSVFQLTDAMLNNQQDKAQHILSQLKSEGVA
MPIILWSLFKELAVLLQLKTAQDKRESLQGLWCKLRIWDKRKPIYQNALNRLELTHIETLLAGASAIELKLKQQGIEDWT
GLSHISLLFDAKAHNKLAHIALD
>Mature_343_residues
MRIYPDQLARQLSPLPQCCLIFGDDPWLCEQSRAALHSEAKRQGFEEKIQLSQETGFSWNELFEQWQAMSLFSSRRIIEL
TLPQAKPGTDGSAMLQTLMQMDNPDVLLILTGPKLAAEQTKSKWFKSLDAKGIYVPCATPEGAQFQRWLDGRINYYGLSL
ARDPREMLFALYEGNLLAADQALQLLQLLSPTAPIDCEQLSQYFEDQSRFSVFQLTDAMLNNQQDKAQHILSQLKSEGVA
MPIILWSLFKELAVLLQLKTAQDKRESLQGLWCKLRIWDKRKPIYQNALNRLELTHIETLLAGASAIELKLKQQGIEDWT
GLSHISLLFDAKAHNKLAHIALD

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=345, Percent_Identity=37.3913043478261, Blast_Score=181, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39045; Mature: 39045

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIYPDQLARQLSPLPQCCLIFGDDPWLCEQSRAALHSEAKRQGFEEKIQLSQETGFSWN
CCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHH
ELFEQWQAMSLFSSRRIIELTLPQAKPGTDGSAMLQTLMQMDNPDVLLILTGPKLAAEQT
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
KSKWFKSLDAKGIYVPCATPEGAQFQRWLDGRINYYGLSLARDPREMLFALYEGNLLAAD
HHHHHHHCCCCCEEEECCCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCEEEHH
QALQLLQLLSPTAPIDCEQLSQYFEDQSRFSVFQLTDAMLNNQQDKAQHILSQLKSEGVA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
MPIILWSLFKELAVLLQLKTAQDKRESLQGLWCKLRIWDKRKPIYQNALNRLELTHIETL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHH
LAGASAIELKLKQQGIEDWTGLSHISLLFDAKAHNKLAHIALD
HHCCHHHHHHHHHCCCHHHCCHHHHHHEEECHHCCCEEEEECC
>Mature Secondary Structure
MRIYPDQLARQLSPLPQCCLIFGDDPWLCEQSRAALHSEAKRQGFEEKIQLSQETGFSWN
CCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHH
ELFEQWQAMSLFSSRRIIELTLPQAKPGTDGSAMLQTLMQMDNPDVLLILTGPKLAAEQT
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
KSKWFKSLDAKGIYVPCATPEGAQFQRWLDGRINYYGLSLARDPREMLFALYEGNLLAAD
HHHHHHHCCCCCEEEECCCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCEEEHH
QALQLLQLLSPTAPIDCEQLSQYFEDQSRFSVFQLTDAMLNNQQDKAQHILSQLKSEGVA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
MPIILWSLFKELAVLLQLKTAQDKRESLQGLWCKLRIWDKRKPIYQNALNRLELTHIETL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHH
LAGASAIELKLKQQGIEDWTGLSHISLLFDAKAHNKLAHIALD
HHCCHHHHHHHHHCCCHHHCCHHHHHHEEECHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 1400251; 8905232; 9278503; 3316191; 8505304; 1575709 [H]