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The map label for this gene is phoQ [H]

Identifier: 157962754

GI number: 157962754

Start: 3596206

End: 3597759

Strand: Direct

Name: phoQ [H]

Synonym: Spea_2936

Alternate gene names: 157962754

Gene position: 3596206-3597759 (Clockwise)

Preceding gene: 157962753

Following gene: 157962755

Centisome position: 69.5

GC content: 48.46

Gene sequence:

>1554_bases
ATGATGCTAAGAAATAACCCGTTGATGATTCGCCTTAAGCACTTACCCAGATTATTACACTCGTTAAAGGCACGCCTCAT
CGTCAGTGCCATGTTGTTGATCTTGGTCTTGCTGCCCATCGTGGGACTCACCCTTAACGATGCCTTTAAATTGCAGGTTA
AAAGTGCCTCGCTCAAAGAGCTTAAAGCCTACGTTTACTCCATCTTGGCGGTGACTGAAGTCGACAATCAGCAAGTGTTT
ATGCCCGAGGTATTATTAGAAAACCAATTTAATGTGATCCAGTCTGGCCTGTACGCCTTGATTACCGTCCCCAATCAGCA
ACAGCCGCCCAAAACCAGTGAGGACAAGCAAGGAACTAACTTCGATGCTAAATTTGTTGGGCCAGACACAGACGAGTCCA
AAGATGAGACGATAATGGCTGAGCAAACCGCTGACGGCAGAAAGGTCGCTTGGCGCTCCAATTCATTGCTTGGCATCGAC
CTCACTTCGGCAATCACCACTGCGGTTCATACTGACCCGGTGTTTCCCCAACCAGAAAAGGGTCAGGGAGAATTCAGCGA
AATTATCCTTAACGGTGAACCCCACTTGGTCTATGGCTTTAGTGTCAGCTTTGAAACGCAACACACAGGGGCTGATGGAA
CAGATGAAAAAACCAGTCGTTTTCCTATCACTGTCTATATCATTAAAGATCAGAGCGACTATCTGCAGCAGGTTAAGGAG
TTTAATCAGCACCTGTGGCGTTGGTTAATGATCTTGATGATTGCCCTTCTCACAATCCAAATATTATGGCTGTGGTGGAC
ACTCAAACCCCTCACTCGCCTGCAGCGTGAATTAGCAGCAATTGAAAACGGTAAAGCCGATCAGCTAGCGGATAATTATC
CCACCGAACTGAGCGCCGTTGCCAAGCAGCTCAATACCTTGCTCAATACCGAGCAAAATCAGCGTAAGCGCTATCGTAAT
GCGCTCGCAGATTTAGCCCATAGCCTTAAAACACCTTTGGCTGTTATTCAAAGTCAGAAAGATTTAAGTCCACAATCATT
TGAGCAGGTCAGCCATATCAATCGCATTATCGGCCACCAGCTCAAACGAGCGCAGACGGCGGCGGGGTCGGCGTGGCACT
TAGGCGTTGAGGTAGTGGAAGTGTCAGATAAGCTGGTGCGTAACCTGCCCAAAATTTACTGCCAGCCTCCCATCACTATC
ACTGAAGAGGTCGACAGGCACGCCGTCTTTAAAGGTGATGCCAGCGATTTAACCGAGATCTTAGGTAATCTGCTGGACAA
TGCCTGTAAGGCTGCTGTCAGCCAAGTGTTATTAAGCGTTAAAAGCGCCGGTGCGAGTTTAGTTATCAGTATCGAAGATG
ACGGTAAAGGCATCGACGAGAGCCTGCACTCTCAGATTTTTGAGCGGGGAATTCGAGCCGACTCCTACCAAACCGGCCAC
GGCATAGGTTTGGCAATCGTTCGCGATCTCGTTGACAGTTATCAAGGGCAACTGCAGGTTAGTCGCTCGGTTAAACTGGG
GGGAGCAAAGTTCGAACTGCGCTTTAACACCTGA

Upstream 100 bases:

>100_bases
GTGTTTATCAGACGCTTACGCAAAAAACTCGACCCAGATAATCAACTCGGCCTTATCGAAACTCTTAGAGGTCAAGGTTA
CCGTCTTGTCTCACTCGATA

Downstream 100 bases:

>100_bases
TCTAACACTTAATCTCACGGCGCTAATCTAGCTGTTTTTTAGCTTTCAGCTTATGTTCATCTTGGCTTTTTTATACTGAT
GTTAACGTATACAAAGGTCA

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MMLRNNPLMIRLKHLPRLLHSLKARLIVSAMLLILVLLPIVGLTLNDAFKLQVKSASLKELKAYVYSILAVTEVDNQQVF
MPEVLLENQFNVIQSGLYALITVPNQQQPPKTSEDKQGTNFDAKFVGPDTDESKDETIMAEQTADGRKVAWRSNSLLGID
LTSAITTAVHTDPVFPQPEKGQGEFSEIILNGEPHLVYGFSVSFETQHTGADGTDEKTSRFPITVYIIKDQSDYLQQVKE
FNQHLWRWLMILMIALLTIQILWLWWTLKPLTRLQRELAAIENGKADQLADNYPTELSAVAKQLNTLLNTEQNQRKRYRN
ALADLAHSLKTPLAVIQSQKDLSPQSFEQVSHINRIIGHQLKRAQTAAGSAWHLGVEVVEVSDKLVRNLPKIYCQPPITI
TEEVDRHAVFKGDASDLTEILGNLLDNACKAAVSQVLLSVKSAGASLVISIEDDGKGIDESLHSQIFERGIRADSYQTGH
GIGLAIVRDLVDSYQGQLQVSRSVKLGGAKFELRFNT

Sequences:

>Translated_517_residues
MMLRNNPLMIRLKHLPRLLHSLKARLIVSAMLLILVLLPIVGLTLNDAFKLQVKSASLKELKAYVYSILAVTEVDNQQVF
MPEVLLENQFNVIQSGLYALITVPNQQQPPKTSEDKQGTNFDAKFVGPDTDESKDETIMAEQTADGRKVAWRSNSLLGID
LTSAITTAVHTDPVFPQPEKGQGEFSEIILNGEPHLVYGFSVSFETQHTGADGTDEKTSRFPITVYIIKDQSDYLQQVKE
FNQHLWRWLMILMIALLTIQILWLWWTLKPLTRLQRELAAIENGKADQLADNYPTELSAVAKQLNTLLNTEQNQRKRYRN
ALADLAHSLKTPLAVIQSQKDLSPQSFEQVSHINRIIGHQLKRAQTAAGSAWHLGVEVVEVSDKLVRNLPKIYCQPPITI
TEEVDRHAVFKGDASDLTEILGNLLDNACKAAVSQVLLSVKSAGASLVISIEDDGKGIDESLHSQIFERGIRADSYQTGH
GIGLAIVRDLVDSYQGQLQVSRSVKLGGAKFELRFNT
>Mature_517_residues
MMLRNNPLMIRLKHLPRLLHSLKARLIVSAMLLILVLLPIVGLTLNDAFKLQVKSASLKELKAYVYSILAVTEVDNQQVF
MPEVLLENQFNVIQSGLYALITVPNQQQPPKTSEDKQGTNFDAKFVGPDTDESKDETIMAEQTADGRKVAWRSNSLLGID
LTSAITTAVHTDPVFPQPEKGQGEFSEIILNGEPHLVYGFSVSFETQHTGADGTDEKTSRFPITVYIIKDQSDYLQQVKE
FNQHLWRWLMILMIALLTIQILWLWWTLKPLTRLQRELAAIENGKADQLADNYPTELSAVAKQLNTLLNTEQNQRKRYRN
ALADLAHSLKTPLAVIQSQKDLSPQSFEQVSHINRIIGHQLKRAQTAAGSAWHLGVEVVEVSDKLVRNLPKIYCQPPITI
TEEVDRHAVFKGDASDLTEILGNLLDNACKAAVSQVLLSVKSAGASLVISIEDDGKGIDESLHSQIFERGIRADSYQTGH
GIGLAIVRDLVDSYQGQLQVSRSVKLGGAKFELRFNT

Specific function: Member of the two-component regulatory system phoQ/phoP which regulates the expression of genes involved in virulence and resistance to host defense antimicrobial peptides. In presence of low periplasmic Mg(2+) concentrations, phoQ functions as a membrane

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787374, Length=333, Percent_Identity=33.6336336336336, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1790551, Length=270, Percent_Identity=28.1481481481481, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788393, Length=243, Percent_Identity=26.3374485596708, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR015014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF08918 PhoQ_Sensor [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57687; Mature: 57687

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLRNNPLMIRLKHLPRLLHSLKARLIVSAMLLILVLLPIVGLTLNDAFKLQVKSASLKE
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHH
LKAYVYSILAVTEVDNQQVFMPEVLLENQFNVIQSGLYALITVPNQQQPPKTSEDKQGTN
HHHHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCC
FDAKFVGPDTDESKDETIMAEQTADGRKVAWRSNSLLGIDLTSAITTAVHTDPVFPQPEK
CCEEECCCCCCCCCCCEEEEECCCCCCEEEEECCCEEEEEHHHHHHHHHCCCCCCCCCCC
GQGEFSEIILNGEPHLVYGFSVSFETQHTGADGTDEKTSRFPITVYIIKDQSDYLQQVKE
CCCCHHHEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHH
FNQHLWRWLMILMIALLTIQILWLWWTLKPLTRLQRELAAIENGKADQLADNYPTELSAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHH
AKQLNTLLNTEQNQRKRYRNALADLAHSLKTPLAVIQSQKDLSPQSFEQVSHINRIIGHQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LKRAQTAAGSAWHLGVEVVEVSDKLVRNLPKIYCQPPITITEEVDRHAVFKGDASDLTEI
HHHHHHCCCCEEEECHHHHHHHHHHHHCCCHHEECCCCCHHHHHCHHHEECCCHHHHHHH
LGNLLDNACKAAVSQVLLSVKSAGASLVISIEDDGKGIDESLHSQIFERGIRADSYQTGH
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCC
GIGLAIVRDLVDSYQGQLQVSRSVKLGGAKFELRFNT
CHHHHHHHHHHHHHCCCEEEECEEEECCEEEEEEECC
>Mature Secondary Structure
MMLRNNPLMIRLKHLPRLLHSLKARLIVSAMLLILVLLPIVGLTLNDAFKLQVKSASLKE
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHH
LKAYVYSILAVTEVDNQQVFMPEVLLENQFNVIQSGLYALITVPNQQQPPKTSEDKQGTN
HHHHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCC
FDAKFVGPDTDESKDETIMAEQTADGRKVAWRSNSLLGIDLTSAITTAVHTDPVFPQPEK
CCEEECCCCCCCCCCCEEEEECCCCCCEEEEECCCEEEEEHHHHHHHHHCCCCCCCCCCC
GQGEFSEIILNGEPHLVYGFSVSFETQHTGADGTDEKTSRFPITVYIIKDQSDYLQQVKE
CCCCHHHEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHH
FNQHLWRWLMILMIALLTIQILWLWWTLKPLTRLQRELAAIENGKADQLADNYPTELSAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHH
AKQLNTLLNTEQNQRKRYRNALADLAHSLKTPLAVIQSQKDLSPQSFEQVSHINRIIGHQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LKRAQTAAGSAWHLGVEVVEVSDKLVRNLPKIYCQPPITITEEVDRHAVFKGDASDLTEI
HHHHHHCCCCEEEECHHHHHHHHHHHHCCCHHEECCCCCHHHHHCHHHEECCCHHHHHHH
LGNLLDNACKAAVSQVLLSVKSAGASLVISIEDDGKGIDESLHSQIFERGIRADSYQTGH
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCC
GIGLAIVRDLVDSYQGQLQVSRSVKLGGAKFELRFNT
CHHHHHHHHHHHHHCCCEEEECEEEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA