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The map label for this gene is mltC [H]

Identifier: 157962545

GI number: 157962545

Start: 3330191

End: 3331750

Strand: Direct

Name: mltC [H]

Synonym: Spea_2724

Alternate gene names: 157962545

Gene position: 3330191-3331750 (Clockwise)

Preceding gene: 157962544

Following gene: 157962546

Centisome position: 64.36

GC content: 43.14

Gene sequence:

>1560_bases
ATGAACTACTATTACAAGTCCCTCATCGCCTTTTCAATTACCTCAATTGTATTTTCAGCTAACGCCAATACTGAGAGTTC
GTTCGCCGAATTAGAGCAGGCTAAAACCCGTATTAACCAACCATTTGAAGAGAAAGTTGCTGATTTTCATCAATACGTTA
ACCAGTATTTGGATGAGTATGAGGCATGGAGATCCCAGTATAATGAAGCTCTCGACAAACAGCGAACCGAGCTCATAGGT
CAATGGGGAAGTGGTGAAGTCTCTGACCAAACAAAAAGTGTCGAATACACTGACAATAACACCGTTAAAAAGGTCATCGA
CTATGAAAATAACACCGCTACCATATCGGTATTACTTGATGCTGATGCTGTTACAGAGTCCACACGTACTATTGTACAAC
AGGGGCTGACCGTCGATGGTCAGGTTTTAAACCTCACGCAGGCCAGCATATCGCTTGTGCAAGTCAATTATTCACTGCAG
CAGGAGAAACAAGAAAAGTCGTTTATTATTAAACAAATAGAGCTGCAAATGAAGGAGCTGGATATTCAAGCAGACAGATT
GATTCAGTCTGAAACAGGTATTCCAGAATCATTTATTTTTGAAAGAGCCCATAAGCAAAAACAGATCTTGCTTGCCGAGG
GCAAAGTCCGCCTTAAGCATATAACTGATCTATATGACAGCAAACGAGAAGAACTAGGAATTGAAACTGTTGCATTAATG
CCCATGGTTTCTGACAGTAAACCAACAACTGCACTGCAGACCGAACAAACACAAGTATCCGATAAGCAAACAACCAAACA
AGCAGAGTTGCGGAATAACGCCACAGACAAGCCAACGGTAACACTCGCAACCGAACAAGCACCAGTTGTCAATAAGCAAA
CAACCTCACAGGTAGAGCGACATGATAAAGCTTCTTTCAAAGAAGCTCAGGCCTTCGCTGAAATTGTGGTCCCAGCCGCA
GTGATTAAGCCTGCAGCCCAGAAAAAAATCGTTTCTTACACAGTAAAACTACCTAATAAGAGCCTAAATCTGCGCGCCAA
CCAGTATCACCCTTTGGCCACTGCAGAAAGCAAGAAGTGGGGAATTGATGAGGCTGTAATTATGGCAATTATGCACAGTG
AATCGAGTTTCAGGCCGGATGCTAAATCTCATGTGCCAGCCTTTGGCTTGATGCAGGTCGTTCCTGTAAGCGCAGGTCAT
GATGTTAACAAGCACATTCGCAACATCGATGCACCAATGAAGGCAGAAGAGTTATATGTACCACCATTAAATGTTGAAAC
TGGTGTCGCTTACCTCCATATATTAAACGACAAGTACCTCAAAGCTATTATTGATCCCCAAAGCCGCCTTTATTGCATGA
TTGCCGCCTACAATACTGGGGCAGGCAATGTAGCTAGAGCCTTCAACGAAAACCGTACGACTAACATCCGCAAGGCCGCA
GAAGTGATTAACAAGATGCCACCGGATCAGGTTTATCAGCACCTGCTAAAACAATTACCGTACGATGAAACCAAAAATTA
CCTCAAAAAGGTGAATCAGCGAATCGCGCTTTACCAATAA

Upstream 100 bases:

>100_bases
GATTTATCCAAGGCGTTAGAATCGCTTAAAGCAATATAAAACAAACTATTAGAGGCTATTTTGATAGCCTCTTTTTATAC
TTGGCATAATAGAGAAAACA

Downstream 100 bases:

>100_bases
TATAAACAGAGATAAAACATATGTTTAAAATTATCCCTATGATGTTGATAGCAATTGGGGTCTATATAGGTATGCAATAC
GATGACGAAATCATCGATCT

Product: lytic transglycosylase

Products: N-Acetylmuramic Acid Residues; N-Acetylglucosamine Residues [C]

Alternate protein names: Murein hydrolase C [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MNYYYKSLIAFSITSIVFSANANTESSFAELEQAKTRINQPFEEKVADFHQYVNQYLDEYEAWRSQYNEALDKQRTELIG
QWGSGEVSDQTKSVEYTDNNTVKKVIDYENNTATISVLLDADAVTESTRTIVQQGLTVDGQVLNLTQASISLVQVNYSLQ
QEKQEKSFIIKQIELQMKELDIQADRLIQSETGIPESFIFERAHKQKQILLAEGKVRLKHITDLYDSKREELGIETVALM
PMVSDSKPTTALQTEQTQVSDKQTTKQAELRNNATDKPTVTLATEQAPVVNKQTTSQVERHDKASFKEAQAFAEIVVPAA
VIKPAAQKKIVSYTVKLPNKSLNLRANQYHPLATAESKKWGIDEAVIMAIMHSESSFRPDAKSHVPAFGLMQVVPVSAGH
DVNKHIRNIDAPMKAEELYVPPLNVETGVAYLHILNDKYLKAIIDPQSRLYCMIAAYNTGAGNVARAFNENRTTNIRKAA
EVINKMPPDQVYQHLLKQLPYDETKNYLKKVNQRIALYQ

Sequences:

>Translated_519_residues
MNYYYKSLIAFSITSIVFSANANTESSFAELEQAKTRINQPFEEKVADFHQYVNQYLDEYEAWRSQYNEALDKQRTELIG
QWGSGEVSDQTKSVEYTDNNTVKKVIDYENNTATISVLLDADAVTESTRTIVQQGLTVDGQVLNLTQASISLVQVNYSLQ
QEKQEKSFIIKQIELQMKELDIQADRLIQSETGIPESFIFERAHKQKQILLAEGKVRLKHITDLYDSKREELGIETVALM
PMVSDSKPTTALQTEQTQVSDKQTTKQAELRNNATDKPTVTLATEQAPVVNKQTTSQVERHDKASFKEAQAFAEIVVPAA
VIKPAAQKKIVSYTVKLPNKSLNLRANQYHPLATAESKKWGIDEAVIMAIMHSESSFRPDAKSHVPAFGLMQVVPVSAGH
DVNKHIRNIDAPMKAEELYVPPLNVETGVAYLHILNDKYLKAIIDPQSRLYCMIAAYNTGAGNVARAFNENRTTNIRKAA
EVINKMPPDQVYQHLLKQLPYDETKNYLKKVNQRIALYQ
>Mature_519_residues
MNYYYKSLIAFSITSIVFSANANTESSFAELEQAKTRINQPFEEKVADFHQYVNQYLDEYEAWRSQYNEALDKQRTELIG
QWGSGEVSDQTKSVEYTDNNTVKKVIDYENNTATISVLLDADAVTESTRTIVQQGLTVDGQVLNLTQASISLVQVNYSLQ
QEKQEKSFIIKQIELQMKELDIQADRLIQSETGIPESFIFERAHKQKQILLAEGKVRLKHITDLYDSKREELGIETVALM
PMVSDSKPTTALQTEQTQVSDKQTTKQAELRNNATDKPTVTLATEQAPVVNKQTTSQVERHDKASFKEAQAFAEIVVPAA
VIKPAAQKKIVSYTVKLPNKSLNLRANQYHPLATAESKKWGIDEAVIMAIMHSESSFRPDAKSHVPAFGLMQVVPVSAGH
DVNKHIRNIDAPMKAEELYVPPLNVETGVAYLHILNDKYLKAIIDPQSRLYCMIAAYNTGAGNVARAFNENRTTNIRKAA
EVINKMPPDQVYQHLLKQLPYDETKNYLKKVNQRIALYQ

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082191, Length=183, Percent_Identity=43.1693989071038, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI87081855, Length=171, Percent_Identity=32.7485380116959, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 58608; Mature: 58608

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYYYKSLIAFSITSIVFSANANTESSFAELEQAKTRINQPFEEKVADFHQYVNQYLDEY
CCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
EAWRSQYNEALDKQRTELIGQWGSGEVSDQTKSVEYTDNNTVKKVIDYENNTATISVLLD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHEECCCCEEEEEEEEE
ADAVTESTRTIVQQGLTVDGQVLNLTQASISLVQVNYSLQQEKQEKSFIIKQIELQMKEL
CHHHHHHHHHHHHCCCCCCCCEEEEHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
DIQADRLIQSETGIPESFIFERAHKQKQILLAEGKVRLKHITDLYDSKREELGIETVALM
CHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCCHHEEEE
PMVSDSKPTTALQTEQTQVSDKQTTKQAELRNNATDKPTVTLATEQAPVVNKQTTSQVER
EEECCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHH
HDKASFKEAQAFAEIVVPAAVIKPAAQKKIVSYTVKLPNKSLNLRANQYHPLATAESKKW
HHHHHHHHHHHHHHHHHHHHHHCHHHHHEEEEEEEECCCCCCCEECCCCCCCCCCCCCCC
GIDEAVIMAIMHSESSFRPDAKSHVPAFGLMQVVPVSAGHDVNKHIRNIDAPMKAEELYV
CCCHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHEECCCCCCHHHHHHHCCCCCCCCCEEC
PPLNVETGVAYLHILNDKYLKAIIDPQSRLYCMIAAYNTGAGNVARAFNENRTTNIRKAA
CCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHCCCCCHHHHHHH
EVINKMPPDQVYQHLLKQLPYDETKNYLKKVNQRIALYQ
HHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNYYYKSLIAFSITSIVFSANANTESSFAELEQAKTRINQPFEEKVADFHQYVNQYLDEY
CCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
EAWRSQYNEALDKQRTELIGQWGSGEVSDQTKSVEYTDNNTVKKVIDYENNTATISVLLD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHEECCCCEEEEEEEEE
ADAVTESTRTIVQQGLTVDGQVLNLTQASISLVQVNYSLQQEKQEKSFIIKQIELQMKEL
CHHHHHHHHHHHHCCCCCCCCEEEEHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
DIQADRLIQSETGIPESFIFERAHKQKQILLAEGKVRLKHITDLYDSKREELGIETVALM
CHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCCHHEEEE
PMVSDSKPTTALQTEQTQVSDKQTTKQAELRNNATDKPTVTLATEQAPVVNKQTTSQVER
EEECCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHH
HDKASFKEAQAFAEIVVPAAVIKPAAQKKIVSYTVKLPNKSLNLRANQYHPLATAESKKW
HHHHHHHHHHHHHHHHHHHHHHCHHHHHEEEEEEEECCCCCCCEECCCCCCCCCCCCCCC
GIDEAVIMAIMHSESSFRPDAKSHVPAFGLMQVVPVSAGHDVNKHIRNIDAPMKAEELYV
CCCHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHEECCCCCCHHHHHHHCCCCCCCCCEEC
PPLNVETGVAYLHILNDKYLKAIIDPQSRLYCMIAAYNTGAGNVARAFNENRTTNIRKAA
CCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHCCCCCHHHHHHH
EVINKMPPDQVYQHLLKQLPYDETKNYLKKVNQRIALYQ
HHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA