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The map label for this gene is mdeA [H]

Identifier: 157962485

GI number: 157962485

Start: 3246844

End: 3248025

Strand: Reverse

Name: mdeA [H]

Synonym: Spea_2664

Alternate gene names: 157962485

Gene position: 3248025-3246844 (Counterclockwise)

Preceding gene: 157962486

Following gene: 157962483

Centisome position: 62.77

GC content: 47.21

Gene sequence:

>1182_bases
ATGCAAGATAGATGGCAATTAGCCACAAAAGTGATCCATGCTGGGCGTGAGGCTAATGAGTCTGGCGCACTCGTGACACC
TTTGTGCCAGAGTGCGACCTTTGTATTTGAGACCGCAGAGCAGGGCGGAGCAAGGTTTGCGGGTGATGAGCAAGGTTATA
TCTATACCCGCTTGGGTAACCCGACGACGGCGGAGCTTGAGCGTAAAATTGCCGCACTCGAGGGAAGCGATGCTGCGGCT
GCTACTGCATCGGGAATGGCGGCGGTTTCTGCAGCCTTGCTGGCAAACTTAAGCCATGGCGATCATTTAGTGGCATCCCA
TGCTGTTTATGGCTGCACCTTTTCCCTTATGACCTCACAACTCGTTAAGTTTGGTATCGAGGTGACGCTAGTTAACTTTA
GCGACGAAACAGAGATTAAAGCGGCAATAAAGCCCAATACCAAAGTGATATTTTGTGAGACACCGGTAAACCCTCATCTC
GATGTGTACAACCTCGATCTGATAGCTGCCATAGCTAAGCAACACAATTTAGTGAGTGTGGTGGATAACACCTTTATGAC
ACCACTACTGCAACAACCGATTAAGCATGGCATCGATATTGTTATTCACAGTGCAACAAAGTACCTCAATGGTCACGGTG
ATGTTATCGCTGGGCTTATTTGTGCCAGCGTTGAACAGATGGAAGTGATTAAGTTTGAAATACTGAAAGATGTTGGCGCG
GTGATTTCGCCCCACGATGCCTGGTTAATTCTACGTGGATTAAAAACCTTAGACGTCCGATTAACCCGACACTGTGATAA
TGCCGAGAAAGTCGCAAGTTACCTTGAGGGTCACCCGAAAGTGTCCAAAGTGTACTACCCGGGTCTTGAAAGTCATCCTG
GACATTCGCTTATAGGCACCCAGATGCGTAGGGGCGGAGGCGTAATAGCCTTCGAGCTAGAAGCAAGTCTTGAACAATCC
ATCGCATTCATTAATCGATTATCACTGTTTTCTATCGCCGTCAGTCTTGGTGATGCGGAGTCATTAATTCAGCACCCAGC
ATCAATGACACATTCACCATACAGTGAAGATGAACGTATTAAGGCAGGTATTAAAGATAACTTACTGAGGATTTCTGTGG
GCTTAGAATCGGTAGAAGATATTATTCAGGCTCTGGAAGATGGCCTAGCGAGTGTAGAGTAG

Upstream 100 bases:

>100_bases
GTTCTTCTGAATGGGTTGGCAAATTGATTTGAGATTGAAGAGATAAAGCTGACATTCACAACGATTAAAAGGTCAGTGCA
TCTCAATCCGGGAGAGCAAT

Downstream 100 bases:

>100_bases
CGGAGAAAGTTGAACACTTGGTACGCAAGTAGTGAAACAGAAATTAGCCCTGCCAAAGATAAGCAGGGCTAATAGTTTGA
ACCTAGCTTTTAGATTTCCC

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MQDRWQLATKVIHAGREANESGALVTPLCQSATFVFETAEQGGARFAGDEQGYIYTRLGNPTTAELERKIAALEGSDAAA
ATASGMAAVSAALLANLSHGDHLVASHAVYGCTFSLMTSQLVKFGIEVTLVNFSDETEIKAAIKPNTKVIFCETPVNPHL
DVYNLDLIAAIAKQHNLVSVVDNTFMTPLLQQPIKHGIDIVIHSATKYLNGHGDVIAGLICASVEQMEVIKFEILKDVGA
VISPHDAWLILRGLKTLDVRLTRHCDNAEKVASYLEGHPKVSKVYYPGLESHPGHSLIGTQMRRGGGVIAFELEASLEQS
IAFINRLSLFSIAVSLGDAESLIQHPASMTHSPYSEDERIKAGIKDNLLRISVGLESVEDIIQALEDGLASVE

Sequences:

>Translated_393_residues
MQDRWQLATKVIHAGREANESGALVTPLCQSATFVFETAEQGGARFAGDEQGYIYTRLGNPTTAELERKIAALEGSDAAA
ATASGMAAVSAALLANLSHGDHLVASHAVYGCTFSLMTSQLVKFGIEVTLVNFSDETEIKAAIKPNTKVIFCETPVNPHL
DVYNLDLIAAIAKQHNLVSVVDNTFMTPLLQQPIKHGIDIVIHSATKYLNGHGDVIAGLICASVEQMEVIKFEILKDVGA
VISPHDAWLILRGLKTLDVRLTRHCDNAEKVASYLEGHPKVSKVYYPGLESHPGHSLIGTQMRRGGGVIAFELEASLEQS
IAFINRLSLFSIAVSLGDAESLIQHPASMTHSPYSEDERIKAGIKDNLLRISVGLESVEDIIQALEDGLASVE
>Mature_393_residues
MQDRWQLATKVIHAGREANESGALVTPLCQSATFVFETAEQGGARFAGDEQGYIYTRLGNPTTAELERKIAALEGSDAAA
ATASGMAAVSAALLANLSHGDHLVASHAVYGCTFSLMTSQLVKFGIEVTLVNFSDETEIKAAIKPNTKVIFCETPVNPHL
DVYNLDLIAAIAKQHNLVSVVDNTFMTPLLQQPIKHGIDIVIHSATKYLNGHGDVIAGLICASVEQMEVIKFEILKDVGA
VISPHDAWLILRGLKTLDVRLTRHCDNAEKVASYLEGHPKVSKVYYPGLESHPGHSLIGTQMRRGGGVIAFELEASLEQS
IAFINRLSLFSIAVSLGDAESLIQHPASMTHSPYSEDERIKAGIKDNLLRISVGLESVEDIIQALEDGLASVE

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=372, Percent_Identity=40.0537634408602, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI299473759, Length=372, Percent_Identity=37.0967741935484, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI25453487, Length=371, Percent_Identity=35.3099730458221, Blast_Score=206, Evalue=2e-53,
Organism=Escherichia coli, GI1790375, Length=383, Percent_Identity=37.3368146214099, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1789383, Length=403, Percent_Identity=29.2803970223325, Blast_Score=172, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17560056, Length=376, Percent_Identity=40.6914893617021, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17538055, Length=375, Percent_Identity=40.8, Blast_Score=271, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI71980708, Length=373, Percent_Identity=40.2144772117962, Blast_Score=270, Evalue=7e-73,
Organism=Saccharomyces cerevisiae, GI6319307, Length=399, Percent_Identity=37.34335839599, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6323333, Length=429, Percent_Identity=32.1678321678322, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6321254, Length=404, Percent_Identity=30.1980198019802, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321170, Length=340, Percent_Identity=31.4705882352941, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6321904, Length=404, Percent_Identity=28.4653465346535, Blast_Score=120, Evalue=5e-28,
Organism=Drosophila melanogaster, GI19922554, Length=388, Percent_Identity=39.4329896907216, Blast_Score=264, Evalue=7e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 42331; Mature: 42331

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDRWQLATKVIHAGREANESGALVTPLCQSATFVFETAEQGGARFAGDEQGYIYTRLGN
CCCHHHHHHHHHHCCCCCCCCCCEEEHHHCCCEEEEEEHHCCCCCCCCCCCCEEEEECCC
PTTAELERKIAALEGSDAAAATASGMAAVSAALLANLSHGDHLVASHAVYGCTFSLMTSQ
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LVKFGIEVTLVNFSDETEIKAAIKPNTKVIFCETPVNPHLDVYNLDLIAAIAKQHNLVSV
HHHCCEEEEEEECCCCCCEEEEECCCCEEEEEECCCCCCCEEECHHHHHHHHHCCCHHHH
VDNTFMTPLLQQPIKHGIDIVIHSATKYLNGHGDVIAGLICASVEQMEVIKFEILKDVGA
HHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VISPHDAWLILRGLKTLDVRLTRHCDNAEKVASYLEGHPKVSKVYYPGLESHPGHSLIGT
HCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHH
QMRRGGGVIAFELEASLEQSIAFINRLSLFSIAVSLGDAESLIQHPASMTHSPYSEDERI
HHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCCCCCCCHHH
KAGIKDNLLRISVGLESVEDIIQALEDGLASVE
HCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQDRWQLATKVIHAGREANESGALVTPLCQSATFVFETAEQGGARFAGDEQGYIYTRLGN
CCCHHHHHHHHHHCCCCCCCCCCEEEHHHCCCEEEEEEHHCCCCCCCCCCCCEEEEECCC
PTTAELERKIAALEGSDAAAATASGMAAVSAALLANLSHGDHLVASHAVYGCTFSLMTSQ
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LVKFGIEVTLVNFSDETEIKAAIKPNTKVIFCETPVNPHLDVYNLDLIAAIAKQHNLVSV
HHHCCEEEEEEECCCCCCEEEEECCCCEEEEEECCCCCCCEEECHHHHHHHHHCCCHHHH
VDNTFMTPLLQQPIKHGIDIVIHSATKYLNGHGDVIAGLICASVEQMEVIKFEILKDVGA
HHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VISPHDAWLILRGLKTLDVRLTRHCDNAEKVASYLEGHPKVSKVYYPGLESHPGHSLIGT
HCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHH
QMRRGGGVIAFELEASLEQSIAFINRLSLFSIAVSLGDAESLIQHPASMTHSPYSEDERI
HHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCCCCCCCHHH
KAGIKDNLLRISVGLESVEDIIQALEDGLASVE
HCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]