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The map label for this gene is putP [H]

Identifier: 157962429

GI number: 157962429

Start: 3176029

End: 3177480

Strand: Direct

Name: putP [H]

Synonym: Spea_2608

Alternate gene names: 157962429

Gene position: 3176029-3177480 (Clockwise)

Preceding gene: 157962428

Following gene: 157962430

Centisome position: 61.38

GC content: 45.87

Gene sequence:

>1452_bases
ATGACAATTGAATTACCTGTTTTAATTACTTTTATCGCCTATCTCTCTTTAATGATGGGCATAGGCTTATGGGCTTATAA
AGCAACAGACTCTGTAGACGATTACATTCTAGGTGGCCGTGGTATGGGGCCTGCGGTTACTGCACTCAGTGTGGGTGCCT
CAGATATGTCGGGCTGGTTACTTTTAGGTTTACCCGGAGCCGTATACCTAGGCGGGTTAGGCGAAGCTTGGATTGGCATA
GGTCTTGTCTTTGGCGCTTGGCTTAACTGGCTATTTGTCGCTAAACGTTTACGTGTTTATACCCAATTTACTGATAATGC
ACTGACACTTCCCGACTTCTTCGAGAAACGTTTTGAAGATAATAAAGGCCTGCTAAAGTTAGTTTCAGCTGTGACCATCT
TAGTCTTCTTCACCTTTTATGCCTCCTCTGGCATGGTAGGTGGTGCGATTTTATTTGAAAAAGTATTCGGTCTAGATTAC
ACCTTGGCTTTGATCATTGGCTCTACCATTATCGTGGCCTATACCTTCGTTGGTGGTTTCTTTGCCGTTAGTTGGACCGA
CTTTTTCCAAGGCTGCCTGATGCTTATTGCACTGTTAATTGTCCCTGTGGCTATTTTTAGTCAGCCAGAGACACAAGCAG
GCTTGGAAAACATCGACCCAGCAATGCTATCTATGTTCAATGAAAATACCACCTTTATTGGCTTCATATCGCTACTAGCA
TGGGGACTAGGCTATTTTGGTCAGCCGCATATTCTGTCTCGCTTTATGGCTATAGCCTCACCTGACGATATTCCGGTTTC
ACGCCGCATCGCCATGGGTTGGATGCTACTAGCCCTTGTCGGTGCGCTGGCAACAGGTATTGCCGGTAGCCTCTACTTTG
CAGCTGAGCCACTATCAAACCCTGAAACCGTGTTCATTCACTTAGCCCATGCAGCATTTAATCCGTGGATTGGTGGTTTA
CTTATCGCAGCTATCTTGTCGGCAATTATGAGTACCATCGATTCGCAGCTTTTAGTCTGTTCGAGTGTGATCACCGAAGA
TTTCTATAAGAAGTGGTTACGTCCACAGGCGACAAGTAAAGAATTGATGCTTGTCGGCCGTATTGGTGTATTGGCGATTG
CCATCATTGCGGGTGTTGTTGCCCTTAATCCAGAAAGCAGCGTACTAGGTTTAGTCAGCTATGCTTGGGCTGGCTTTGGT
GCAGCTTTCGGTCCTGTCGTGTTGTTATCTCTATTTTGGCGTGGTTACAGCCGCAATGGTGCAGTCGCGACCATTATTGT
TGGTGCACTGACAGTTGTGGTTTGGAAGCAACTTTCAGGTCATATTTTTGACATCTATGAAATCCTTCCAGGCTTCATTC
TAGCCAGCCTAGCCGGTGTGGTTATCAGCAAGATGCAAAGCCCATCAGAGACAATTAAACAGCAGTTCGATAGCTTCGAA
GCCAAGCTATAG

Upstream 100 bases:

>100_bases
TAGATCTTCATCACATTCCCTCGCTTTATTGGGCCTCAGCTCATAGATCTGCTAGCATAGCGCCCACACAAATTGAAAAT
AAAAGAAGCGTGCATCACAC

Downstream 100 bases:

>100_bases
CTGCAATAGAGAGCAGAACAGATAAAAAGCGTACACTTGTACGCTTTTTTTATTTTTACCCATCAGCGCCTAAACCATCA
ACGACTTACATCCCATTAAC

Product: sodium/proline symporter

Products: NA

Alternate protein names: Proline permease; Propionate transporter [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTIELPVLITFIAYLSLMMGIGLWAYKATDSVDDYILGGRGMGPAVTALSVGASDMSGWLLLGLPGAVYLGGLGEAWIGI
GLVFGAWLNWLFVAKRLRVYTQFTDNALTLPDFFEKRFEDNKGLLKLVSAVTILVFFTFYASSGMVGGAILFEKVFGLDY
TLALIIGSTIIVAYTFVGGFFAVSWTDFFQGCLMLIALLIVPVAIFSQPETQAGLENIDPAMLSMFNENTTFIGFISLLA
WGLGYFGQPHILSRFMAIASPDDIPVSRRIAMGWMLLALVGALATGIAGSLYFAAEPLSNPETVFIHLAHAAFNPWIGGL
LIAAILSAIMSTIDSQLLVCSSVITEDFYKKWLRPQATSKELMLVGRIGVLAIAIIAGVVALNPESSVLGLVSYAWAGFG
AAFGPVVLLSLFWRGYSRNGAVATIIVGALTVVVWKQLSGHIFDIYEILPGFILASLAGVVISKMQSPSETIKQQFDSFE
AKL

Sequences:

>Translated_483_residues
MTIELPVLITFIAYLSLMMGIGLWAYKATDSVDDYILGGRGMGPAVTALSVGASDMSGWLLLGLPGAVYLGGLGEAWIGI
GLVFGAWLNWLFVAKRLRVYTQFTDNALTLPDFFEKRFEDNKGLLKLVSAVTILVFFTFYASSGMVGGAILFEKVFGLDY
TLALIIGSTIIVAYTFVGGFFAVSWTDFFQGCLMLIALLIVPVAIFSQPETQAGLENIDPAMLSMFNENTTFIGFISLLA
WGLGYFGQPHILSRFMAIASPDDIPVSRRIAMGWMLLALVGALATGIAGSLYFAAEPLSNPETVFIHLAHAAFNPWIGGL
LIAAILSAIMSTIDSQLLVCSSVITEDFYKKWLRPQATSKELMLVGRIGVLAIAIIAGVVALNPESSVLGLVSYAWAGFG
AAFGPVVLLSLFWRGYSRNGAVATIIVGALTVVVWKQLSGHIFDIYEILPGFILASLAGVVISKMQSPSETIKQQFDSFE
AKL
>Mature_482_residues
TIELPVLITFIAYLSLMMGIGLWAYKATDSVDDYILGGRGMGPAVTALSVGASDMSGWLLLGLPGAVYLGGLGEAWIGIG
LVFGAWLNWLFVAKRLRVYTQFTDNALTLPDFFEKRFEDNKGLLKLVSAVTILVFFTFYASSGMVGGAILFEKVFGLDYT
LALIIGSTIIVAYTFVGGFFAVSWTDFFQGCLMLIALLIVPVAIFSQPETQAGLENIDPAMLSMFNENTTFIGFISLLAW
GLGYFGQPHILSRFMAIASPDDIPVSRRIAMGWMLLALVGALATGIAGSLYFAAEPLSNPETVFIHLAHAAFNPWIGGLL
IAAILSAIMSTIDSQLLVCSSVITEDFYKKWLRPQATSKELMLVGRIGVLAIAIIAGVVALNPESSVLGLVSYAWAGFGA
AFGPVVLLSLFWRGYSRNGAVATIIVGALTVVVWKQLSGHIFDIYEILPGFILASLAGVVISKMQSPSETIKQQFDSFEA
KL

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=488, Percent_Identity=46.3114754098361, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI4507035, Length=367, Percent_Identity=26.158038147139, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI11141885, Length=451, Percent_Identity=25.7206208425721, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI17941285, Length=408, Percent_Identity=27.2058823529412, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1787251, Length=498, Percent_Identity=53.4136546184739, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI87082237, Length=461, Percent_Identity=28.1995661605206, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1790503, Length=463, Percent_Identity=25.2699784017279, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17539284, Length=406, Percent_Identity=23.8916256157635, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533094, Length=422, Percent_Identity=24.8815165876777, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24645928, Length=394, Percent_Identity=26.3959390862944, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI221459584, Length=388, Percent_Identity=23.7113402061856, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24651739, Length=479, Percent_Identity=23.5908141962422, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640370, Length=358, Percent_Identity=26.2569832402235, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648033, Length=400, Percent_Identity=24, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21356865, Length=400, Percent_Identity=24, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221459586, Length=385, Percent_Identity=23.3766233766234, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281362918, Length=200, Percent_Identity=29, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 51995; Mature: 51864

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIELPVLITFIAYLSLMMGIGLWAYKATDSVDDYILGGRGMGPAVTALSVGASDMSGWL
CEEEHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHEECCCCCCHHHHHHHCCCCCCCCEE
LLGLPGAVYLGGLGEAWIGIGLVFGAWLNWLFVAKRLRVYTQFTDNALTLPDFFEKRFED
EEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
NKGLLKLVSAVTILVFFTFYASSGMVGGAILFEKVFGLDYTLALIIGSTIIVAYTFVGGF
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
FAVSWTDFFQGCLMLIALLIVPVAIFSQPETQAGLENIDPAMLSMFNENTTFIGFISLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHCCHHHHHHHCCCCHHHHHHHHHH
WGLGYFGQPHILSRFMAIASPDDIPVSRRIAMGWMLLALVGALATGIAGSLYFAAEPLSN
HHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
PETVFIHLAHAAFNPWIGGLLIAAILSAIMSTIDSQLLVCSSVITEDFYKKWLRPQATSK
CCEEEEEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ELMLVGRIGVLAIAIIAGVVALNPESSVLGLVSYAWAGFGAAFGPVVLLSLFWRGYSRNG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AVATIIVGALTVVVWKQLSGHIFDIYEILPGFILASLAGVVISKMQSPSETIKQQFDSFE
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AKL
CCC
>Mature Secondary Structure 
TIELPVLITFIAYLSLMMGIGLWAYKATDSVDDYILGGRGMGPAVTALSVGASDMSGWL
EEEHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHEECCCCCCHHHHHHHCCCCCCCCEE
LLGLPGAVYLGGLGEAWIGIGLVFGAWLNWLFVAKRLRVYTQFTDNALTLPDFFEKRFED
EEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
NKGLLKLVSAVTILVFFTFYASSGMVGGAILFEKVFGLDYTLALIIGSTIIVAYTFVGGF
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
FAVSWTDFFQGCLMLIALLIVPVAIFSQPETQAGLENIDPAMLSMFNENTTFIGFISLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHCCHHHHHHHCCCCHHHHHHHHHH
WGLGYFGQPHILSRFMAIASPDDIPVSRRIAMGWMLLALVGALATGIAGSLYFAAEPLSN
HHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
PETVFIHLAHAAFNPWIGGLLIAAILSAIMSTIDSQLLVCSSVITEDFYKKWLRPQATSK
CCEEEEEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ELMLVGRIGVLAIAIIAGVVALNPESSVLGLVSYAWAGFGAAFGPVVLLSLFWRGYSRNG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AVATIIVGALTVVVWKQLSGHIFDIYEILPGFILASLAGVVISKMQSPSETIKQQFDSFE
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AKL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3302614; 8905232; 9278503; 1400239; 3325781; 3053687; 3053649; 1567896; 8119910 [H]