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The map label for this gene is hcaT [H]

Identifier: 157962408

GI number: 157962408

Start: 3150053

End: 3151216

Strand: Reverse

Name: hcaT [H]

Synonym: Spea_2587

Alternate gene names: 157962408

Gene position: 3151216-3150053 (Counterclockwise)

Preceding gene: 157962409

Following gene: 157962407

Centisome position: 60.9

GC content: 47.77

Gene sequence:

>1164_bases
ATGTTTCAACCCAAAAGCACAGAGCAGCAACTTCGCTGGCTCTGTGCCTGTTATTTCTTTTTCTTTTCCATCTTGGGCAT
CATGGTGCCTTATCTGGGAGTCTTCTTCGACAGCCGTGGTTTTGATGCTCAAGAAATAGGCTTGCTACTCGCCATTTTAA
TGGCTACGCGGATCATCGCGCCCAATCTATGGGCTATCGTTGCCGACAGAACGGGTATGCGCTCCGAGCTGATTAAAATC
GGTGCTGGCGCAGCGGCGATAAGCTATCTAACATTTTTCTATGATGGCAGCTTAACCTATCTGGCCATCAGTCTAGCTGT
GTATACCTTCTTCTGGAACGCCATTTTGGCGCAGCTTGAGGTGATCACCCTCGAAACCTTAGGTGACGATACTAATCGAT
ATGGCGCTATTCGTAGTTGGGGCAGTATAGGTTATATCGTACTGGTGATCAGCGGCGGCTTCGCCATCGATCACTTTGGG
CCGCAGATCTTGCCCTATATCGGCATGATGCTATTTATCGGTATGCTAGTCAGCGCCTTACCACTACCGTCAAATCGCGC
CGTTGTCGATAAGACCCAAAAGCGCAAGCCTTTAGTCTTCGATAGAGCCCTCATCTGGTTTTTGATTTCAGCCATGCTGT
TGCAGATGAGCGCAGGGCCTTTCTATGGCTTCTTCGTTTTGTATTTAAAGCAAGCTGGTTACACAGAGGCTACGGCGGGG
ATTTTCGTTGCATTGGGCGTGATGGCCGAGATTGTCATGTTTATGTATGCGCCTAAGTTGCTTGGTCGATTTGGGGTTAA
CCTCCTGTTAGTCGTGAGCATAGGCTTAACCGCAGTGCGTTGGTTATTACTCGCATACGGCGTTGAAAATATTGTCTACC
TTAGTATCAGTCAGTTGCTGCACGCCTTTACCTTCGGCTTAACCCATGCAGCATCGATTCAATTTATTCATAAACATTTC
GATATTAATCGTCGCAGTCAGGGTCAAGCACTCTATGCCAGTTTAAGTTTTGGTGTGGGTGGCGCAATAGGCACTTGGCT
TTGTGGCATCATCTGGGGAGATGGCTCTGGCGCAGTGTCTACCTGGATTTTTGCTGCAGCCTGTGCATTTTTATCTATGC
TAGCCGTATTTTTTATTCCTAAACCCAAAGCGACGGTTGCTTAA

Upstream 100 bases:

>100_bases
CCGAAGCCATGTTGGCTATCGTGCTGATGGATCACTTATTACGTCACCGCGCACAAAATATGGATGTGAATAGCAGTACA
CCTATTATCGGAATGCGTTG

Downstream 100 bases:

>100_bases
CCAGTACAACATAAAAAATGGAGCATCATTGTGGGGAAGTTTCGTCTTGGTTTAATCATTAATCCACTAGCAGGTCTTGG
TGGCAGTGTTGCCTTAAAAG

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MFQPKSTEQQLRWLCACYFFFFSILGIMVPYLGVFFDSRGFDAQEIGLLLAILMATRIIAPNLWAIVADRTGMRSELIKI
GAGAAAISYLTFFYDGSLTYLAISLAVYTFFWNAILAQLEVITLETLGDDTNRYGAIRSWGSIGYIVLVISGGFAIDHFG
PQILPYIGMMLFIGMLVSALPLPSNRAVVDKTQKRKPLVFDRALIWFLISAMLLQMSAGPFYGFFVLYLKQAGYTEATAG
IFVALGVMAEIVMFMYAPKLLGRFGVNLLLVVSIGLTAVRWLLLAYGVENIVYLSISQLLHAFTFGLTHAASIQFIHKHF
DINRRSQGQALYASLSFGVGGAIGTWLCGIIWGDGSGAVSTWIFAAACAFLSMLAVFFIPKPKATVA

Sequences:

>Translated_387_residues
MFQPKSTEQQLRWLCACYFFFFSILGIMVPYLGVFFDSRGFDAQEIGLLLAILMATRIIAPNLWAIVADRTGMRSELIKI
GAGAAAISYLTFFYDGSLTYLAISLAVYTFFWNAILAQLEVITLETLGDDTNRYGAIRSWGSIGYIVLVISGGFAIDHFG
PQILPYIGMMLFIGMLVSALPLPSNRAVVDKTQKRKPLVFDRALIWFLISAMLLQMSAGPFYGFFVLYLKQAGYTEATAG
IFVALGVMAEIVMFMYAPKLLGRFGVNLLLVVSIGLTAVRWLLLAYGVENIVYLSISQLLHAFTFGLTHAASIQFIHKHF
DINRRSQGQALYASLSFGVGGAIGTWLCGIIWGDGSGAVSTWIFAAACAFLSMLAVFFIPKPKATVA
>Mature_387_residues
MFQPKSTEQQLRWLCACYFFFFSILGIMVPYLGVFFDSRGFDAQEIGLLLAILMATRIIAPNLWAIVADRTGMRSELIKI
GAGAAAISYLTFFYDGSLTYLAISLAVYTFFWNAILAQLEVITLETLGDDTNRYGAIRSWGSIGYIVLVISGGFAIDHFG
PQILPYIGMMLFIGMLVSALPLPSNRAVVDKTQKRKPLVFDRALIWFLISAMLLQMSAGPFYGFFVLYLKQAGYTEATAG
IFVALGVMAEIVMFMYAPKLLGRFGVNLLLVVSIGLTAVRWLLLAYGVENIVYLSISQLLHAFTFGLTHAASIQFIHKHF
DINRRSQGQALYASLSFGVGGAIGTWLCGIIWGDGSGAVSTWIFAAACAFLSMLAVFFIPKPKATVA

Specific function: Probable permease involved in the uptake of 3- phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family. HcaT subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788886, Length=347, Percent_Identity=25.0720461095101, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR016196
- InterPro:   IPR005276 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 42517; Mature: 42517

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQPKSTEQQLRWLCACYFFFFSILGIMVPYLGVFFDSRGFDAQEIGLLLAILMATRIIA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
PNLWAIVADRTGMRSELIKIGAGAAAISYLTFFYDGSLTYLAISLAVYTFFWNAILAQLE
CCHHEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
VITLETLGDDTNRYGAIRSWGSIGYIVLVISGGFAIDHFGPQILPYIGMMLFIGMLVSAL
HEEEECCCCCCCCCCCHHCCCCCCEEEEEEECCEEEHHCCCHHHHHHHHHHHHHHHHHHC
PLPSNRAVVDKTQKRKPLVFDRALIWFLISAMLLQMSAGPFYGFFVLYLKQAGYTEATAG
CCCCCCCHHCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHH
IFVALGVMAEIVMFMYAPKLLGRFGVNLLLVVSIGLTAVRWLLLAYGVENIVYLSISQLL
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAFTFGLTHAASIQFIHKHFDINRRSQGQALYASLSFGVGGAIGTWLCGIIWGDGSGAVS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHEECCCCCHHH
TWIFAAACAFLSMLAVFFIPKPKATVA
HHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MFQPKSTEQQLRWLCACYFFFFSILGIMVPYLGVFFDSRGFDAQEIGLLLAILMATRIIA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
PNLWAIVADRTGMRSELIKIGAGAAAISYLTFFYDGSLTYLAISLAVYTFFWNAILAQLE
CCHHEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
VITLETLGDDTNRYGAIRSWGSIGYIVLVISGGFAIDHFGPQILPYIGMMLFIGMLVSAL
HEEEECCCCCCCCCCCHHCCCCCCEEEEEEECCEEEHHCCCHHHHHHHHHHHHHHHHHHC
PLPSNRAVVDKTQKRKPLVFDRALIWFLISAMLLQMSAGPFYGFFVLYLKQAGYTEATAG
CCCCCCCHHCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHH
IFVALGVMAEIVMFMYAPKLLGRFGVNLLLVVSIGLTAVRWLLLAYGVENIVYLSISQLL
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAFTFGLTHAASIQFIHKHFDINRRSQGQALYASLSFGVGGAIGTWLCGIIWGDGSGAVS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHEECCCCCHHH
TWIFAAACAFLSMLAVFFIPKPKATVA
HHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9603882; 9205837; 9278503 [H]