Click here to switch to the map view.

The map label for this gene is asnB [H]

Identifier: 157962404

GI number: 157962404

Start: 3146167

End: 3147831

Strand: Direct

Name: asnB [H]

Synonym: Spea_2583

Alternate gene names: 157962404

Gene position: 3146167-3147831 (Clockwise)

Preceding gene: 157962403

Following gene: 157962411

Centisome position: 60.8

GC content: 47.09

Gene sequence:

>1665_bases
ATGTGTTCAATTTTTGCAATTTTAGATATTCAATCCGACGCGGCTCCACTGCGTCAAGTCGCTCTAGAGATGTCCAAGCT
TCTGCGTCATCGCGGTCCAGATTGGTCTGGGATTTACAGCAGTGATAAAGCAATTTTAGCCCATGAACGTTTAGCTATTG
TCGATATTGAACACGGCGCACAGCCACTGCTTAGCCAAGATGGCTCTATCGTATTGGCCGTAAACGGTGAGATCTACAAC
CACAAAGAGCTTAAGGCCGAGCTTGGTGATAAGTACAGTTACCAAACAAACTCAGACTGCGAAGTGATCTTGGCTCTTTA
TCAAGAGTATGGCACTGAATTCTTAGATAAACTCAACGGAATTTTCGCCTTTGTGCTTTACGACAAGTCAAAAGACACGT
ATCTGGTTGGCCGCGATCACATGGGTATTATTCCGCTCTACACCGGCCACGATGCGGAAGGTAACTTCTACGTTGCATCT
GAGATGAAAGCACTGATGCCAGTATGTAAAACTGTCGAAACCTTTACTCCAGGCCATTACCTGTCAAGTGAGAAAGGCGA
GCTTACCCAATACTATCAACGTGACTGGCGCGATTTTGCCGCAGTGCAAGATAACCCTGCCAGCGCGGAAGATGTGCGTG
ATGCATTAGAAGCTGCAGTAAAACGCCAGCTAATGTCTGATGTACCTTACGGTGTATTACTCTCTGGTGGATTGGATTCA
TCAGTGATTTCAGCCATCACTCAAACGTTCGCCAAACGTCGTATCGAAGATGACGGTGAAAGCAACGCATGGTGGCCACA
GCTTCACTCATTTGCCGTCGGTCTTGAAGGTGCACCTGACTTAATTGCTGCAAAAAAGGTAGCCGATGCCATTGGTACCA
TTCACCATGAAATTACCTTTACCTTCCAAGATGGTATCGATGCGATTAAAGATGTGATTTACCACTTAGAAACTTACGAT
GTCACCACTATTCGAGCGGCTACTCCTATGTACTTGATGGCACGTAAGATCAAGGCTATGGGCATTAAGATGGTGTTATC
GGGTGAAGGCGCCGATGAGTTGTTCGGCGGCTACCTCTACTTCCATAAAGCGCCAAACGCACAAGCCTTCCATGAAGAGC
TTGTTCGTAAACTCGATAAACTACACCTATTCGACTGTTTGCGCGCTAACAAAGCGATGGCGGCATGGGGACTCGAAGCT
CGCGTACCTTTCCTAGACAAAGAGTTTATGGATGTCGCGATGCGCACTAACCCTGATGCAAAAATGTCGAAGAACGGTAA
AATTGAAAAGCATATCCTGCGTGAAGCCTTTGAGCACAAGTTACCTAAGGAAGTAGCATGGCGTCAAAAGGAGCAGTTCA
GTGACGGTGTAGGTTACTCTTGGATCGATGGCCTTAAAGATCACGCAGCAGAGAGAGTGGACGATTTAAAATTGGCAAAC
GCTAAGTTTAGATTCCCATACAACACGCCAGAAACCAAAGAAGCTTATTTCTACCGTACCTTCTTTGAAGAGCTGTTCCC
ATTACCAAGCGCAGCAGAAACGGTACCAGGTGGTAAATCAGTAGCCTGCTCGACTCCAGAAGCCTTAGCGTGGGATGCAA
GCCTGCAAGGCATAATCGACCCATCGGGCCGAGCGGTACAATCGGTACACGACAGCGCCTACTAA

Upstream 100 bases:

>100_bases
CTTTTAACAATGATTACTCAAAAGCCGGTTTGTATTCTTAAGTATTCGATGGCAGAGTAATACTCAATTGAGTTTAAGAC
AGAATTTTTTGGAGTGACCT

Downstream 100 bases:

>100_bases
GATAAGTTCTGAACACAAAAAAGCCAGTCGGGAGTTCCAACTGGCTTAATAAAACCATCAACTTATTTTAGAATTTAGAC
AGGCTAGCCTTTTACCCTAA

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MCSIFAILDIQSDAAPLRQVALEMSKLLRHRGPDWSGIYSSDKAILAHERLAIVDIEHGAQPLLSQDGSIVLAVNGEIYN
HKELKAELGDKYSYQTNSDCEVILALYQEYGTEFLDKLNGIFAFVLYDKSKDTYLVGRDHMGIIPLYTGHDAEGNFYVAS
EMKALMPVCKTVETFTPGHYLSSEKGELTQYYQRDWRDFAAVQDNPASAEDVRDALEAAVKRQLMSDVPYGVLLSGGLDS
SVISAITQTFAKRRIEDDGESNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEITFTFQDGIDAIKDVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRTNPDAKMSKNGKIEKHILREAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKDHAAERVDDLKLAN
AKFRFPYNTPETKEAYFYRTFFEELFPLPSAAETVPGGKSVACSTPEALAWDASLQGIIDPSGRAVQSVHDSAY

Sequences:

>Translated_554_residues
MCSIFAILDIQSDAAPLRQVALEMSKLLRHRGPDWSGIYSSDKAILAHERLAIVDIEHGAQPLLSQDGSIVLAVNGEIYN
HKELKAELGDKYSYQTNSDCEVILALYQEYGTEFLDKLNGIFAFVLYDKSKDTYLVGRDHMGIIPLYTGHDAEGNFYVAS
EMKALMPVCKTVETFTPGHYLSSEKGELTQYYQRDWRDFAAVQDNPASAEDVRDALEAAVKRQLMSDVPYGVLLSGGLDS
SVISAITQTFAKRRIEDDGESNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEITFTFQDGIDAIKDVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRTNPDAKMSKNGKIEKHILREAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKDHAAERVDDLKLAN
AKFRFPYNTPETKEAYFYRTFFEELFPLPSAAETVPGGKSVACSTPEALAWDASLQGIIDPSGRAVQSVHDSAY
>Mature_554_residues
MCSIFAILDIQSDAAPLRQVALEMSKLLRHRGPDWSGIYSSDKAILAHERLAIVDIEHGAQPLLSQDGSIVLAVNGEIYN
HKELKAELGDKYSYQTNSDCEVILALYQEYGTEFLDKLNGIFAFVLYDKSKDTYLVGRDHMGIIPLYTGHDAEGNFYVAS
EMKALMPVCKTVETFTPGHYLSSEKGELTQYYQRDWRDFAAVQDNPASAEDVRDALEAAVKRQLMSDVPYGVLLSGGLDS
SVISAITQTFAKRRIEDDGESNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEITFTFQDGIDAIKDVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRTNPDAKMSKNGKIEKHILREAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKDHAAERVDDLKLAN
AKFRFPYNTPETKEAYFYRTFFEELFPLPSAAETVPGGKSVACSTPEALAWDASLQGIIDPSGRAVQSVHDSAY

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=543, Percent_Identity=40.3314917127072, Blast_Score=346, Evalue=4e-95,
Organism=Homo sapiens, GI168229252, Length=543, Percent_Identity=40.3314917127072, Blast_Score=346, Evalue=4e-95,
Organism=Homo sapiens, GI168229250, Length=543, Percent_Identity=40.3314917127072, Blast_Score=346, Evalue=4e-95,
Organism=Homo sapiens, GI296010848, Length=514, Percent_Identity=41.0505836575875, Blast_Score=343, Evalue=3e-94,
Organism=Homo sapiens, GI296010852, Length=449, Percent_Identity=42.0935412026726, Blast_Score=312, Evalue=7e-85,
Organism=Homo sapiens, GI296010850, Length=449, Percent_Identity=42.0935412026726, Blast_Score=312, Evalue=7e-85,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=70.0361010830325, Blast_Score=831, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=570, Percent_Identity=42.1052631578947, Blast_Score=427, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71993933, Length=559, Percent_Identity=42.2182468694097, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17560178, Length=520, Percent_Identity=36.9230769230769, Blast_Score=306, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI25147560, Length=311, Percent_Identity=38.9067524115756, Blast_Score=200, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6321563, Length=545, Percent_Identity=51.3761467889908, Blast_Score=550, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6325403, Length=580, Percent_Identity=49.6551724137931, Blast_Score=538, Evalue=1e-154,
Organism=Drosophila melanogaster, GI45553209, Length=573, Percent_Identity=47.1204188481675, Blast_Score=496, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 61939; Mature: 61939

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFAILDIQSDAAPLRQVALEMSKLLRHRGPDWSGIYSSDKAILAHERLAIVDIEHGA
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEEECCCC
QPLLSQDGSIVLAVNGEIYNHKELKAELGDKYSYQTNSDCEVILALYQEYGTEFLDKLNG
CHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
IFAFVLYDKSKDTYLVGRDHMGIIPLYTGHDAEGNFYVASEMKALMPVCKTVETFTPGHY
EEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
LSSEKGELTQYYQRDWRDFAAVQDNPASAEDVRDALEAAVKRQLMSDVPYGVLLSGGLDS
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SVISAITQTFAKRRIEDDGESNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEITF
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEE
TFQDGIDAIKDVIYHLETYDVTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLY
EEHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEARVPFLDKEFMDVAMRTNPDA
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCC
KMSKNGKIEKHILREAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKDHAAERVDDLKLAN
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECC
AKFRFPYNTPETKEAYFYRTFFEELFPLPSAAETVPGGKSVACSTPEALAWDASLQGIID
CEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCCCEECCCCCCEEECCCCCCCCC
PSGRAVQSVHDSAY
CCCHHHHHHHHCCC
>Mature Secondary Structure
MCSIFAILDIQSDAAPLRQVALEMSKLLRHRGPDWSGIYSSDKAILAHERLAIVDIEHGA
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEEECCCC
QPLLSQDGSIVLAVNGEIYNHKELKAELGDKYSYQTNSDCEVILALYQEYGTEFLDKLNG
CHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
IFAFVLYDKSKDTYLVGRDHMGIIPLYTGHDAEGNFYVASEMKALMPVCKTVETFTPGHY
EEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
LSSEKGELTQYYQRDWRDFAAVQDNPASAEDVRDALEAAVKRQLMSDVPYGVLLSGGLDS
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SVISAITQTFAKRRIEDDGESNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEITF
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEE
TFQDGIDAIKDVIYHLETYDVTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLY
EEHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEARVPFLDKEFMDVAMRTNPDA
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCC
KMSKNGKIEKHILREAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKDHAAERVDDLKLAN
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECC
AKFRFPYNTPETKEAYFYRTFFEELFPLPSAAETVPGGKSVACSTPEALAWDASLQGIID
CEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCCCEECCCCCCEEECCCCCCCCC
PSGRAVQSVHDSAY
CCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]