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The map label for this gene is rnt [H]

Identifier: 157962392

GI number: 157962392

Start: 3131172

End: 3131870

Strand: Direct

Name: rnt [H]

Synonym: Spea_2571

Alternate gene names: 157962392

Gene position: 3131172-3131870 (Clockwise)

Preceding gene: 157962381

Following gene: 157962393

Centisome position: 60.51

GC content: 47.21

Gene sequence:

>699_bases
ATGACTGACACTTTCGACCCTAATTTAATGAAAAACCGCTTTAGAGGGTATTTCCCAGTGGTCATTGATGTTGAAACCGC
AGGTTTTAACGCTAAAACAGATGCGCTGCTAGAAATTGCAGTCAGCATGCTAAAGATGGACGATGAAGGTGTGCTTGCTC
TAGATAAGACATTGCATTTCAATATTGAACCGTTTGAAGGTGCTAACCTTGAGCCAGAAGCATTAGCCTTTACGGGGATC
GACCCCACCAGCCCTCTTCGCGGCGCTGTTAGCGAAAAAGAAGCTTTCTTAGAAATTTTTAAAGAAGTCAAAAAAGCGCA
AAAAGCTGCGGGTTGTCACCGCAGTATTATTGTTGCTCACAACGCGGCATTCGATCACGGCTTTGTTACCCAAGCGATCG
AACGTAATGCACTAAAGCGTACTCCTTTCCACCCCTTTGCCACATTTGACACCGCAGCGCTTGCGGGCTTAGCCATAGGT
CATACTGTACTCGCAAAGGCCTGCGCGATGGCTGGGATCCCTTTCGATAACCGTGAAGCCCACTCAGCACTTTATGACAC
CGAGCGCACGGCTGAGCTATTTTGCTTAATCGTCAACCGCTGGAAGGCCCTAGGTGGTTGGCCTTTTATTGCTGATGAAA
CAGAAGAGGCTGACACCAACTCCTCTGAAGAAAAAGCTGAGGACTACACTAAAAATTAG

Upstream 100 bases:

>100_bases
AAAGATTTATCTTTCTTTAGCCTATAAATCAATCAAACGCACATGACAGTGATTATCAGTTGCAGCATAATAGTCGCCTG
CACACTTTTGGGAAATTTTA

Downstream 100 bases:

>100_bases
TCACTCGAGAGTGAATCACAAGAACAAAAAAGCTGCGATAATCGCAGCTTTTTTCATGCTTAAACATGTCCCGTCCTGAG
ATAAGTTGACACATTGGAGA

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MTDTFDPNLMKNRFRGYFPVVIDVETAGFNAKTDALLEIAVSMLKMDDEGVLALDKTLHFNIEPFEGANLEPEALAFTGI
DPTSPLRGAVSEKEAFLEIFKEVKKAQKAAGCHRSIIVAHNAAFDHGFVTQAIERNALKRTPFHPFATFDTAALAGLAIG
HTVLAKACAMAGIPFDNREAHSALYDTERTAELFCLIVNRWKALGGWPFIADETEEADTNSSEEKAEDYTKN

Sequences:

>Translated_232_residues
MTDTFDPNLMKNRFRGYFPVVIDVETAGFNAKTDALLEIAVSMLKMDDEGVLALDKTLHFNIEPFEGANLEPEALAFTGI
DPTSPLRGAVSEKEAFLEIFKEVKKAQKAAGCHRSIIVAHNAAFDHGFVTQAIERNALKRTPFHPFATFDTAALAGLAIG
HTVLAKACAMAGIPFDNREAHSALYDTERTAELFCLIVNRWKALGGWPFIADETEEADTNSSEEKAEDYTKN
>Mature_231_residues
TDTFDPNLMKNRFRGYFPVVIDVETAGFNAKTDALLEIAVSMLKMDDEGVLALDKTLHFNIEPFEGANLEPEALAFTGID
PTSPLRGAVSEKEAFLEIFKEVKKAQKAAGCHRSIIVAHNAAFDHGFVTQAIERNALKRTPFHPFATFDTAALAGLAIGH
TVLAKACAMAGIPFDNREAHSALYDTERTAELFCLIVNRWKALGGWPFIADETEEADTNSSEEKAEDYTKN

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=213, Percent_Identity=69.4835680751174, Blast_Score=303, Evalue=7e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 25513; Mature: 25382

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTFDPNLMKNRFRGYFPVVIDVETAGFNAKTDALLEIAVSMLKMDDEGVLALDKTLHF
CCCCCCCHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEECEEEE
NIEPFEGANLEPEALAFTGIDPTSPLRGAVSEKEAFLEIFKEVKKAQKAAGCHRSIIVAH
ECCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
NAAFDHGFVTQAIERNALKRTPFHPFATFDTAALAGLAIGHTVLAKACAMAGIPFDNREA
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
HSALYDTERTAELFCLIVNRWKALGGWPFIADETEEADTNSSEEKAEDYTKN
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
TDTFDPNLMKNRFRGYFPVVIDVETAGFNAKTDALLEIAVSMLKMDDEGVLALDKTLHF
CCCCCCHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEECEEEE
NIEPFEGANLEPEALAFTGIDPTSPLRGAVSEKEAFLEIFKEVKKAQKAAGCHRSIIVAH
ECCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
NAAFDHGFVTQAIERNALKRTPFHPFATFDTAALAGLAIGHTVLAKACAMAGIPFDNREA
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
HSALYDTERTAELFCLIVNRWKALGGWPFIADETEEADTNSSEEKAEDYTKN
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA