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The map label for this gene is ligA [H]

Identifier: 157962332

GI number: 157962332

Start: 3059563

End: 3060438

Strand: Reverse

Name: ligA [H]

Synonym: Spea_2511

Alternate gene names: 157962332

Gene position: 3060438-3059563 (Counterclockwise)

Preceding gene: 157962336

Following gene: 157962330

Centisome position: 59.14

GC content: 42.12

Gene sequence:

>876_bases
ATGTTATCAATTCGGTTTATCAATTCATTTCTCAGGCTTTGTTTAATCAGCCTCATCACTGTCAATTATAGCTACGCCAA
TAACACAGACATTCGAACTCCAAATAAACCTGCTATTCAACTTGCATCTGCATTCGACGAGTCAATTTCGGATATAACGC
AATACTTGGTGAGCGAAAAACTCGATGGTGTTAGGGGCTACTGGGATGGCAAAGTGATGTACAGCCGTTCCGGCCGCCAA
ATTGCATTACCCGATTGGTTTGTAGCGGGCTTTCCTAGCTACCCTTTAGATGGTGAGTTATGGATTAAACGAGGGATGTT
TGAGCAAGTATCGGCAATGGTTAGAAGCTCCAAGGTGAGTGATGAAGAGTGGGCCAGAGTTAAATTTATGATTTTTGACT
TACCCGCCGAGTCCAGTGTTTTTGAATTGCGCTACCGAAAGGCTGTAAAAGAATTGGCTGGTATCAGTCCATACTTAAAG
GTGATTGGTCAAAATCAGTTCGATAGTTTTCAGGCGTTAGAGGCAGAGTTACAGAAGGTCATTGCGAAAAACGGTGAAGG
ACTCATGCTTCATAAGAAACATGCTTTTTATAAATCAGGTCGTAGCAAAGACATAGTTAAACTAAAACCTTTTTATGATG
CTGAAGCAAGAGTCATAGGTTATACGTCTGGTAAGGGGCGTTTTAGTGGCATGATGGGAGCCTTGGTGGTTAAAAATCAG
CAGGGGATCACATTCAACTTAGGAACAGGGTTCAGTAAAGAACAAAGGCAGAGTCCACCACAGATTGGCAGCTTAGTGAC
ATATAAGTATTACGGGCTGACACAAAAAGGTACACCAAGATTTGCGAGCTTTTTAAGGGTGAGACATGCTAAATAG

Upstream 100 bases:

>100_bases
GTTTGGCGAGATAAGATAAGTCAGGCTAGTCTTAAGTTATATCAAGTAAGGCACAATACGTGTAGCACAGGATGTGCAGA
TTAACGCAAGGAGCGGCATT

Downstream 100 bases:

>100_bases
TGAATATTGAAATGAGAAGTCAGGTTCGAGGAAAAAGCCTCGAACCTTTATCTGCTAAGCTACTACATATCAGCCTAGTT
ATACTTTAATTGTGCTTTAG

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLSIRFINSFLRLCLISLITVNYSYANNTDIRTPNKPAIQLASAFDESISDITQYLVSEKLDGVRGYWDGKVMYSRSGRQ
IALPDWFVAGFPSYPLDGELWIKRGMFEQVSAMVRSSKVSDEEWARVKFMIFDLPAESSVFELRYRKAVKELAGISPYLK
VIGQNQFDSFQALEAELQKVIAKNGEGLMLHKKHAFYKSGRSKDIVKLKPFYDAEARVIGYTSGKGRFSGMMGALVVKNQ
QGITFNLGTGFSKEQRQSPPQIGSLVTYKYYGLTQKGTPRFASFLRVRHAK

Sequences:

>Translated_291_residues
MLSIRFINSFLRLCLISLITVNYSYANNTDIRTPNKPAIQLASAFDESISDITQYLVSEKLDGVRGYWDGKVMYSRSGRQ
IALPDWFVAGFPSYPLDGELWIKRGMFEQVSAMVRSSKVSDEEWARVKFMIFDLPAESSVFELRYRKAVKELAGISPYLK
VIGQNQFDSFQALEAELQKVIAKNGEGLMLHKKHAFYKSGRSKDIVKLKPFYDAEARVIGYTSGKGRFSGMMGALVVKNQ
QGITFNLGTGFSKEQRQSPPQIGSLVTYKYYGLTQKGTPRFASFLRVRHAK
>Mature_291_residues
MLSIRFINSFLRLCLISLITVNYSYANNTDIRTPNKPAIQLASAFDESISDITQYLVSEKLDGVRGYWDGKVMYSRSGRQ
IALPDWFVAGFPSYPLDGELWIKRGMFEQVSAMVRSSKVSDEEWARVKFMIFDLPAESSVFELRYRKAVKELAGISPYLK
VIGQNQFDSFQALEAELQKVIAKNGEGLMLHKKHAFYKSGRSKDIVKLKPFYDAEARVIGYTSGKGRFSGMMGALVVKNQ
QGITFNLGTGFSKEQRQSPPQIGSLVTYKYYGLTQKGTPRFASFLRVRHAK

Specific function: Catalyzes efficient strand joining on a single nicked DNA [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 32943; Mature: 32943

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSIRFINSFLRLCLISLITVNYSYANNTDIRTPNKPAIQLASAFDESISDITQYLVSEK
CEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LDGVRGYWDGKVMYSRSGRQIALPDWFVAGFPSYPLDGELWIKRGMFEQVSAMVRSSKVS
CCCCCCEECCEEEEECCCCEEECCCHHCCCCCCCCCCCHHHHHHCHHHHHHHHHHHCCCC
DEEWARVKFMIFDLPAESSVFELRYRKAVKELAGISPYLKVIGQNQFDSFQALEAELQKV
CCHHCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHH
IAKNGEGLMLHKKHAFYKSGRSKDIVKLKPFYDAEARVIGYTSGKGRFSGMMGALVVKNQ
HHCCCCEEEEEEHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCCHHHHCEEEEEEECC
QGITFNLGTGFSKEQRQSPPQIGSLVTYKYYGLTQKGTPRFASFLRVRHAK
CCEEEECCCCCCHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLSIRFINSFLRLCLISLITVNYSYANNTDIRTPNKPAIQLASAFDESISDITQYLVSEK
CEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LDGVRGYWDGKVMYSRSGRQIALPDWFVAGFPSYPLDGELWIKRGMFEQVSAMVRSSKVS
CCCCCCEECCEEEEECCCCEEECCCHHCCCCCCCCCCCHHHHHHCHHHHHHHHHHHCCCC
DEEWARVKFMIFDLPAESSVFELRYRKAVKELAGISPYLKVIGQNQFDSFQALEAELQKV
CCHHCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHH
IAKNGEGLMLHKKHAFYKSGRSKDIVKLKPFYDAEARVIGYTSGKGRFSGMMGALVVKNQ
HHCCCCEEEEEEHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCCHHHHCEEEEEEECC
QGITFNLGTGFSKEQRQSPPQIGSLVTYKYYGLTQKGTPRFASFLRVRHAK
CCEEEECCCCCCHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550458; 7542800; 9060431 [H]