Click here to switch to the map view.

The map label for this gene is mltD [H]

Identifier: 157962228

GI number: 157962228

Start: 2935508

End: 2937067

Strand: Direct

Name: mltD [H]

Synonym: Spea_2407

Alternate gene names: 157962228

Gene position: 2935508-2937067 (Clockwise)

Preceding gene: 157962227

Following gene: 157962232

Centisome position: 56.73

GC content: 46.35

Gene sequence:

>1560_bases
ATGCGCGTACCATTTTTATTTGTCGCAGGGGGTATTGCTCTGTTGACAGGTTGTCAAACTTTAGACAACCAACAGACTTC
GGAAGGGATCCCTGCCAAGCCAGTTGCAGTTGAGAAAGTCGCCCCTAAGCCTATTGAGCCAGAGATTGTTGAGGTCGTTG
AAGTCACCGATATTTGGCAACGAATTCGTCTCAATATGCAGATGGAAGTCCCCGATGAAAAACTCGTTCGCCAATATCGC
GACTGGTATATAAAACATCCTCAACATTTAGAGCGCGTATCTGAGCGAGCGACGCCATTTATGTATCTGATTGTAGATGA
GATCGAGAAGCGTAATCTACCAATCGAGTTGGCTCTGCTACCCATTGTTGAGAGTGCTTTCGATCCTTTTGCCTACTCAC
ATGGTGCAGCATCAGGCCTTTGGCAATTCACTTCGCCAATGGCACGTCACTTCGGCCTACAGATGAACTGGTGGTATGAC
GGTCGCAGAGATGTCCCCGCCGCCACGATTGCCGCTTTAGATATGCTTGAGTACCTTCATGGTAAAACGGGGCAAAATTG
GTTATATGCCATTGCCGCCTACAATACAGGTGAAGGACGCGTGATTAACGCGGCTAAGCGCAACAAGAAAAAAGGATTAT
CGACTGATTTCTTTAGTTTAAATCTTCCAAGAGAAACTGAGCGCTATGTTCCTCAGTTACTTGCGTTAGCCGACGTGATC
AAAAATGCCGATAAATATGGCATTAAACTGACCCCCATCAGCAATGAGCCACAAATTGAAGTCATCAATATTGGTAGTCA
AATCGATTTAGCGCTTGCCGCTGACATTGCCAATATGACCACATCAGAGCTACACAAGTTAAACCCAGGTTTTAACCGTT
GGGCCACGGCTCCTGATGGGCCACACAAACTTGTATTGCCCGTTGATAAAGCCGATGCCTTTAAACTGGCATTAAATGAC
ACTAGCACAACCGATCGCCTCAACTGGGAACGCTATAAGATTGAGTCAGGCGACAGCTTAGGATTAATCGCAAAACGTTA
CCGCACTACGATTTCGGCATTGAAGGCAGTCAATGATATCAATGGCAACACCATTGTTGCCGGAAAGTACCTGTTGATCC
CCGTTGCAGCGAAGAACCTTGATGAATACCTGTTGTCAGCCGATCAACGTCTAGCGCGTCAACAAAATAAAGCCCGTGGT
GCCAGTAAAATTACCTATAAGGTCAGATCCGGTGATTCTCTGTGGAAAATTGCCAAGCAGCACAAGGTGAAAACGGCCCA
ACTTGCCAGCTGGAATAGCATGGCACCTAAAGATCCGCTGCAGATTGGCCAGAAGCTCGTCATCTGGTCTGGTACCGTAT
CTAACAAGAGTGGCTTAAGCGAAGTGATGCGTACCGTAAATTATAAGGTCCGTAATGGTGACTCTTTGGCTCGCATCGCC
AATAAGTTCAATGTCAGCGTTAGCGACCTCGTTCGTTGGAACTCACTCGAAAAGAGCAAGTATATTCAACCCGGTCAAAT
GCTTAAGCTGTATGTTGATATGACAAAAGTCAGAACATAA

Upstream 100 bases:

>100_bases
CAATTTCTAAAAAATAATCTACAATAGCCCGCTATTCTAGCGCCACTAGGCCAGATTTACTCTGGTTTTTTTGAGCCTAA
ATTAAGGACACTGATTTTAG

Downstream 100 bases:

>100_bases
GTTGAACCCATTAAAAAAGGCGACCTTAGGTCGCCTTTTTTATAGCTATGACTTGGTTAACCAGTTAGTAGCTGTATATT
ACAGGAAGCATGGCTGGCAC

Product: lytic transglycosylase

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MRVPFLFVAGGIALLTGCQTLDNQQTSEGIPAKPVAVEKVAPKPIEPEIVEVVEVTDIWQRIRLNMQMEVPDEKLVRQYR
DWYIKHPQHLERVSERATPFMYLIVDEIEKRNLPIELALLPIVESAFDPFAYSHGAASGLWQFTSPMARHFGLQMNWWYD
GRRDVPAATIAALDMLEYLHGKTGQNWLYAIAAYNTGEGRVINAAKRNKKKGLSTDFFSLNLPRETERYVPQLLALADVI
KNADKYGIKLTPISNEPQIEVINIGSQIDLALAADIANMTTSELHKLNPGFNRWATAPDGPHKLVLPVDKADAFKLALND
TSTTDRLNWERYKIESGDSLGLIAKRYRTTISALKAVNDINGNTIVAGKYLLIPVAAKNLDEYLLSADQRLARQQNKARG
ASKITYKVRSGDSLWKIAKQHKVKTAQLASWNSMAPKDPLQIGQKLVIWSGTVSNKSGLSEVMRTVNYKVRNGDSLARIA
NKFNVSVSDLVRWNSLEKSKYIQPGQMLKLYVDMTKVRT

Sequences:

>Translated_519_residues
MRVPFLFVAGGIALLTGCQTLDNQQTSEGIPAKPVAVEKVAPKPIEPEIVEVVEVTDIWQRIRLNMQMEVPDEKLVRQYR
DWYIKHPQHLERVSERATPFMYLIVDEIEKRNLPIELALLPIVESAFDPFAYSHGAASGLWQFTSPMARHFGLQMNWWYD
GRRDVPAATIAALDMLEYLHGKTGQNWLYAIAAYNTGEGRVINAAKRNKKKGLSTDFFSLNLPRETERYVPQLLALADVI
KNADKYGIKLTPISNEPQIEVINIGSQIDLALAADIANMTTSELHKLNPGFNRWATAPDGPHKLVLPVDKADAFKLALND
TSTTDRLNWERYKIESGDSLGLIAKRYRTTISALKAVNDINGNTIVAGKYLLIPVAAKNLDEYLLSADQRLARQQNKARG
ASKITYKVRSGDSLWKIAKQHKVKTAQLASWNSMAPKDPLQIGQKLVIWSGTVSNKSGLSEVMRTVNYKVRNGDSLARIA
NKFNVSVSDLVRWNSLEKSKYIQPGQMLKLYVDMTKVRT
>Mature_519_residues
MRVPFLFVAGGIALLTGCQTLDNQQTSEGIPAKPVAVEKVAPKPIEPEIVEVVEVTDIWQRIRLNMQMEVPDEKLVRQYR
DWYIKHPQHLERVSERATPFMYLIVDEIEKRNLPIELALLPIVESAFDPFAYSHGAASGLWQFTSPMARHFGLQMNWWYD
GRRDVPAATIAALDMLEYLHGKTGQNWLYAIAAYNTGEGRVINAAKRNKKKGLSTDFFSLNLPRETERYVPQLLALADVI
KNADKYGIKLTPISNEPQIEVINIGSQIDLALAADIANMTTSELHKLNPGFNRWATAPDGPHKLVLPVDKADAFKLALND
TSTTDRLNWERYKIESGDSLGLIAKRYRTTISALKAVNDINGNTIVAGKYLLIPVAAKNLDEYLLSADQRLARQQNKARG
ASKITYKVRSGDSLWKIAKQHKVKTAQLASWNSMAPKDPLQIGQKLVIWSGTVSNKSGLSEVMRTVNYKVRNGDSLARIA
NKFNVSVSDLVRWNSLEKSKYIQPGQMLKLYVDMTKVRT

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=449, Percent_Identity=36.0801781737194, Blast_Score=279, Evalue=3e-76,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 58328; Mature: 58328

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVPFLFVAGGIALLTGCQTLDNQQTSEGIPAKPVAVEKVAPKPIEPEIVEVVEVTDIWQ
CCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHH
RIRLNMQMEVPDEKLVRQYRDWYIKHPQHLERVSERATPFMYLIVDEIEKRNLPIELALL
HHHCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEHHHHCCCCEEEEEH
PIVESAFDPFAYSHGAASGLWQFTSPMARHFGLQMNWWYDGRRDVPAATIAALDMLEYLH
HHHHHHCCCCHHCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHC
GKTGQNWLYAIAAYNTGEGRVINAAKRNKKKGLSTDFFSLNLPRETERYVPQLLALADVI
CCCCCCEEEEEEEEECCCCCEEEHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
KNADKYGIKLTPISNEPQIEVINIGSQIDLALAADIANMTTSELHKLNPGFNRWATAPDG
HCCHHCCEEEEECCCCCCEEEEECCCCEEHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCC
PHKLVLPVDKADAFKLALNDTSTTDRLNWERYKIESGDSLGLIAKRYRTTISALKAVNDI
CCEEEEEECCCCEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
NGNTIVAGKYLLIPVAAKNLDEYLLSADQRLARQQNKARGASKITYKVRSGDSLWKIAKQ
CCCEEEECCEEEEEEHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
HKVKTAQLASWNSMAPKDPLQIGQKLVIWSGTVSNKSGLSEVMRTVNYKVRNGDSLARIA
HCCCHHHHCCCCCCCCCCHHHHCCEEEEEECCCCCHHHHHHHHHHHCEEECCCCHHHHHH
NKFNVSVSDLVRWNSLEKSKYIQPGQMLKLYVDMTKVRT
HHCCCCHHHHHHHCCCCHHHCCCCCHHEEEEEEHHHHCC
>Mature Secondary Structure
MRVPFLFVAGGIALLTGCQTLDNQQTSEGIPAKPVAVEKVAPKPIEPEIVEVVEVTDIWQ
CCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHH
RIRLNMQMEVPDEKLVRQYRDWYIKHPQHLERVSERATPFMYLIVDEIEKRNLPIELALL
HHHCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEHHHHCCCCEEEEEH
PIVESAFDPFAYSHGAASGLWQFTSPMARHFGLQMNWWYDGRRDVPAATIAALDMLEYLH
HHHHHHCCCCHHCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHC
GKTGQNWLYAIAAYNTGEGRVINAAKRNKKKGLSTDFFSLNLPRETERYVPQLLALADVI
CCCCCCEEEEEEEEECCCCCEEEHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
KNADKYGIKLTPISNEPQIEVINIGSQIDLALAADIANMTTSELHKLNPGFNRWATAPDG
HCCHHCCEEEEECCCCCCEEEEECCCCEEHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCC
PHKLVLPVDKADAFKLALNDTSTTDRLNWERYKIESGDSLGLIAKRYRTTISALKAVNDI
CCEEEEEECCCCEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
NGNTIVAGKYLLIPVAAKNLDEYLLSADQRLARQQNKARGASKITYKVRSGDSLWKIAKQ
CCCEEEECCEEEEEEHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
HKVKTAQLASWNSMAPKDPLQIGQKLVIWSGTVSNKSGLSEVMRTVNYKVRNGDSLARIA
HCCCHHHHCCCCCCCCCCHHHHCCEEEEEECCCCCHHHHHHHHHHHCEEECCCCHHHHHH
NKFNVSVSDLVRWNSLEKSKYIQPGQMLKLYVDMTKVRT
HHCCCCHHHHHHHCCCCHHHCCCCCHHEEEEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]