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The map label for this gene is gloB

Identifier: 157962227

GI number: 157962227

Start: 2934626

End: 2935417

Strand: Direct

Name: gloB

Synonym: Spea_2406

Alternate gene names: 157962227

Gene position: 2934626-2935417 (Clockwise)

Preceding gene: 157962224

Following gene: 157962228

Centisome position: 56.71

GC content: 43.43

Gene sequence:

>792_bases
ATGTTACATATCACTCCATTAGCCGCGTTCAATGATAATTATATCTGGGTGTTCCAATCTCAGCAAAATACTGGTGCCGA
AAGTAGTGGCATCTACGTCGTAGATCCTGGTGATGGCCAAGTTGTCATCGACTACTTAATCCAAACAGGACAGGCACTGC
TAGGCATACTCATCACCCACCATCATCATGATCACACCGGTGGGATCGAACAGTTGATCCGTCGATTTGGTGCACAAATT
GCCGTTTATGGACCGCAAAGCGAGAATATTATCGGTGTAAATCACCCTATCGTGGCAAACGGTGACATCTCCCTCACAAA
TGCAGGTTTAAATGCAAAAGTTATTCAGCTACCCGGCCATACCTTAGGTCATATCGCTTATTTAATCGAAGATGTTCTAT
TTTGTGGTGATACTCTGTTTAGTGCTGGCTGTGGTCGTATTTTTGAAGGCAGTGCCGAGCAGATGTACCAATCACTCAGT
GAGCTAGGTCAGCTGCCTGACAATACCAAAGTTTGCTGTGCTCACGAATACACCATAGCGAATCTAGCATTTGCTAACAG
GGTTGAGCCTAATAATGCAGCCCTCATAGATTACACAAAAAAAGCCCAAGCCCTTCGAGCTCAAAATCTGCCGACCTTGC
CTTCATCAATTGGACAGGAAAAGGCGATTAACCCCTTTTTAAGAAGCGATAGTGAAGAAATTTGCCAATCTTTATCCATT
CAATTCCAACAACCTATCAATAATCCATTGCAAAGCTTCTCACTTCTACGCCAATGGAAAGACAATTTCTAA

Upstream 100 bases:

>100_bases
GAAAGCGGTATACAAGCAATAGCTTTGCTCAGTAAACTAACTAAAGTGCTCAATTTATTTTGCTGGAGCGCTTAATTATA
TTAAATGAGAGAGTTTAGAC

Downstream 100 bases:

>100_bases
AAAATAATCTACAATAGCCCGCTATTCTAGCGCCACTAGGCCAGATTTACTCTGGTTTTTTTGAGCCTAAATTAAGGACA
CTGATTTTAGATGCGCGTAC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MLHITPLAAFNDNYIWVFQSQQNTGAESSGIYVVDPGDGQVVIDYLIQTGQALLGILITHHHHDHTGGIEQLIRRFGAQI
AVYGPQSENIIGVNHPIVANGDISLTNAGLNAKVIQLPGHTLGHIAYLIEDVLFCGDTLFSAGCGRIFEGSAEQMYQSLS
ELGQLPDNTKVCCAHEYTIANLAFANRVEPNNAALIDYTKKAQALRAQNLPTLPSSIGQEKAINPFLRSDSEEICQSLSI
QFQQPINNPLQSFSLLRQWKDNF

Sequences:

>Translated_263_residues
MLHITPLAAFNDNYIWVFQSQQNTGAESSGIYVVDPGDGQVVIDYLIQTGQALLGILITHHHHDHTGGIEQLIRRFGAQI
AVYGPQSENIIGVNHPIVANGDISLTNAGLNAKVIQLPGHTLGHIAYLIEDVLFCGDTLFSAGCGRIFEGSAEQMYQSLS
ELGQLPDNTKVCCAHEYTIANLAFANRVEPNNAALIDYTKKAQALRAQNLPTLPSSIGQEKAINPFLRSDSEEICQSLSI
QFQQPINNPLQSFSLLRQWKDNF
>Mature_263_residues
MLHITPLAAFNDNYIWVFQSQQNTGAESSGIYVVDPGDGQVVIDYLIQTGQALLGILITHHHHDHTGGIEQLIRRFGAQI
AVYGPQSENIIGVNHPIVANGDISLTNAGLNAKVIQLPGHTLGHIAYLIEDVLFCGDTLFSAGCGRIFEGSAEQMYQSLS
ELGQLPDNTKVCCAHEYTIANLAFANRVEPNNAALIDYTKKAQALRAQNLPTLPSSIGQEKAINPFLRSDSEEICQSLSI
QFQQPINNPLQSFSLLRQWKDNF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=270, Percent_Identity=35.5555555555556, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI94538322, Length=270, Percent_Identity=35.5555555555556, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI14150041, Length=240, Percent_Identity=36.6666666666667, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI21703352, Length=235, Percent_Identity=36.5957446808511, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI116642887, Length=235, Percent_Identity=36.5957446808511, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI46361987, Length=198, Percent_Identity=37.8787878787879, Blast_Score=121, Evalue=7e-28,
Organism=Escherichia coli, GI1786406, Length=262, Percent_Identity=43.1297709923664, Blast_Score=204, Evalue=4e-54,
Organism=Escherichia coli, GI1787158, Length=183, Percent_Identity=28.9617486338798, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17536925, Length=274, Percent_Identity=35.036496350365, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6320478, Length=247, Percent_Identity=26.7206477732794, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324614, Length=258, Percent_Identity=26.3565891472868, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21356335, Length=270, Percent_Identity=37.7777777777778, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24667703, Length=270, Percent_Identity=37.7777777777778, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24667711, Length=270, Percent_Identity=37.7777777777778, Blast_Score=179, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_SHEPA (A8H589)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001502261.1
- ProteinModelPortal:   A8H589
- SMR:   A8H589
- STRING:   A8H589
- GeneID:   5662799
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_2406
- NMPDR:   fig|398579.3.peg.2071
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK982151
- BioCyc:   SPEA398579:SPEA_2406-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28825; Mature: 28825

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHITPLAAFNDNYIWVFQSQQNTGAESSGIYVVDPGDGQVVIDYLIQTGQALLGILITH
CEEEEEEEEECCCEEEEEECCCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHC
HHHDHTGGIEQLIRRFGAQIAVYGPQSENIIGVNHPIVANGDISLTNAGLNAKVIQLPGH
CCCCCCCHHHHHHHHHCCEEEEECCCCCCEEECCCCEEECCCEEEEECCCCEEEEECCCC
TLGHIAYLIEDVLFCGDTLFSAGCGRIFEGSAEQMYQSLSELGQLPDNTKVCCAHEYTIA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHH
NLAFANRVEPNNAALIDYTKKAQALRAQNLPTLPSSIGQEKAINPFLRSDSEEICQSLSI
HHHHHCCCCCCCCEEEEEHHHHHHHHHCCCCCCHHHCCHHHCCCHHHCCCHHHHHHHHCC
QFQQPINNPLQSFSLLRQWKDNF
HHHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLHITPLAAFNDNYIWVFQSQQNTGAESSGIYVVDPGDGQVVIDYLIQTGQALLGILITH
CEEEEEEEEECCCEEEEEECCCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHC
HHHDHTGGIEQLIRRFGAQIAVYGPQSENIIGVNHPIVANGDISLTNAGLNAKVIQLPGH
CCCCCCCHHHHHHHHHCCEEEEECCCCCCEEECCCCEEECCCEEEEECCCCEEEEECCCC
TLGHIAYLIEDVLFCGDTLFSAGCGRIFEGSAEQMYQSLSELGQLPDNTKVCCAHEYTIA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHH
NLAFANRVEPNNAALIDYTKKAQALRAQNLPTLPSSIGQEKAINPFLRSDSEEICQSLSI
HHHHHCCCCCCCCEEEEEHHHHHHHHHCCCCCCHHHCCHHHCCCHHHCCCHHHHHHHHCC
QFQQPINNPLQSFSLLRQWKDNF
HHHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA