Click here to switch to the map view.

The map label for this gene is rnhA [H]

Identifier: 157962224

GI number: 157962224

Start: 2932212

End: 2932769

Strand: Direct

Name: rnhA [H]

Synonym: Spea_2403

Alternate gene names: 157962224

Gene position: 2932212-2932769 (Clockwise)

Preceding gene: 157962219

Following gene: 157962227

Centisome position: 56.67

GC content: 46.77

Gene sequence:

>558_bases
ATGAATTTATACATTTCTCAGTTAAATTTTTTGGGTATACTCGCCCGCAATTATAGCTATGGTAGCAACTTGATGACTGG
ACTGAAACAGATCTCTATCTATACCGACGGTTCTTGCCTAGGAAATCCGGGCCCTGGCGGTTATGGCATCGTTTTAAAGT
ACAAAAAACGCACCAAAGAACTGGCCGATGGTTTTGCCCTCACCACCAATAATCGTATGGAGATGCTTGCGCCGATTATA
GCCCTCGAAGCGCTAAAAGTGCCATGCGAGGTCATATTAACCAGCGATAGCCAATATATGCGTCAAGGGATCACCCAGTG
GATCCATGGTTGGAAACGTAAAGGGTGGATGACTTCAACAAACCAGCCCGTGAAAAATGTCGATCTTTGGAAGCGTTTAG
ATACAGTATCACAACGCCACCAAGTTGAATGGCGCTGGGTAAAAGGCCACGCAGGACATAGCGAGAACGAACGCTGTGAC
GATCTTGCGCGCCAAGCTGCAGAGGCTAAGCCCACACAAGAAGATAACGGGTACCTAGCTCAGCAAAAACAAGACTAA

Upstream 100 bases:

>100_bases
TAATTCGATGGCCTAAATAAATTGGGCCACTCGATTGGTTCATACCTGTGGTTTCGGTATCGAGAATAATTTGCCGCTTG
GCACTTGAAACAATATTCAT

Downstream 100 bases:

>100_bases
TAATAAACTCGGCCTAGAACCTGTCACGCTGGCTGGTTCTAAGCTTTATCTTTAGAACTTATCTTTGGAACATTAGTGCT
CCGTATTAAGAAAAGTTCGG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MNLYISQLNFLGILARNYSYGSNLMTGLKQISIYTDGSCLGNPGPGGYGIVLKYKKRTKELADGFALTTNNRMEMLAPII
ALEALKVPCEVILTSDSQYMRQGITQWIHGWKRKGWMTSTNQPVKNVDLWKRLDTVSQRHQVEWRWVKGHAGHSENERCD
DLARQAAEAKPTQEDNGYLAQQKQD

Sequences:

>Translated_185_residues
MNLYISQLNFLGILARNYSYGSNLMTGLKQISIYTDGSCLGNPGPGGYGIVLKYKKRTKELADGFALTTNNRMEMLAPII
ALEALKVPCEVILTSDSQYMRQGITQWIHGWKRKGWMTSTNQPVKNVDLWKRLDTVSQRHQVEWRWVKGHAGHSENERCD
DLARQAAEAKPTQEDNGYLAQQKQD
>Mature_185_residues
MNLYISQLNFLGILARNYSYGSNLMTGLKQISIYTDGSCLGNPGPGGYGIVLKYKKRTKELADGFALTTNNRMEMLAPII
ALEALKVPCEVILTSDSQYMRQGITQWIHGWKRKGWMTSTNQPVKNVDLWKRLDTVSQRHQVEWRWVKGHAGHSENERCD
DLARQAAEAKPTQEDNGYLAQQKQD

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=143, Percent_Identity=33.5664335664336, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1786408, Length=151, Percent_Identity=66.887417218543, Blast_Score=226, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI115532542, Length=142, Percent_Identity=37.3239436619718, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI17137476, Length=162, Percent_Identity=36.4197530864198, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45552509, Length=162, Percent_Identity=36.4197530864198, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 21020; Mature: 21020

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLYISQLNFLGILARNYSYGSNLMTGLKQISIYTDGSCLGNPGPGGYGIVLKYKKRTKE
CCEEHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCEEEEEEEHHHHHH
LADGFALTTNNRMEMLAPIIALEALKVPCEVILTSDSQYMRQGITQWIHGWKRKGWMTST
HHCCEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCC
NQPVKNVDLWKRLDTVSQRHQVEWRWVKGHAGHSENERCDDLARQAAEAKPTQEDNGYLA
CCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
QQKQD
CCCCC
>Mature Secondary Structure
MNLYISQLNFLGILARNYSYGSNLMTGLKQISIYTDGSCLGNPGPGGYGIVLKYKKRTKE
CCEEHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCEEEEEEEHHHHHH
LADGFALTTNNRMEMLAPIIALEALKVPCEVILTSDSQYMRQGITQWIHGWKRKGWMTST
HHCCEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCC
NQPVKNVDLWKRLDTVSQRHQVEWRWVKGHAGHSENERCDDLARQAAEAKPTQEDNGYLA
CCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
QQKQD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA