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The map label for this gene is ansA [H]

Identifier: 157962075

GI number: 157962075

Start: 2734394

End: 2735410

Strand: Direct

Name: ansA [H]

Synonym: Spea_2254

Alternate gene names: 157962075

Gene position: 2734394-2735410 (Clockwise)

Preceding gene: 157962074

Following gene: 157962079

Centisome position: 52.84

GC content: 45.72

Gene sequence:

>1017_bases
ATGACAAAACGATCTATCTATGTAGCCTACACTGGCGGTACAATCGGTATGCAAAAAACCGATCATGGTTTCGCACCTGT
TGCAAATTTCCTCACAGGCTGCGTTAAAGCTATGCCTGAGTTTTATCATGACGAAATGCCAGACTTTGTCATTAATGAAT
ATTCGCCTCTTATCGACTCTGCGAATATGAAACCAACCGATTGGCAAATGATTGCCAATGATATTAAAGCAAAATACGAT
CAGTATGATGGTTTCGTTATTCTCCATGGTACCGACACCATGGCTTTCACCGCATCAGCGCTCTCTTTTATGCTACAAGG
CTTACAAAAGCCTGTCATCGTAACCGGTTCGCAAATCCCTCTTGCCCAATTGCGTTCAGATGGACAAACCAATTTACTCA
ACGCCTTGTATATCGCGGCCAACTATCCAGTTGCCGAAGTTTGCCTATTCTTTAATAACAAACTCTTTAGAGGTAACCGC
ACCACCAAGGTCCATGCCGATGGCTTCAATGCCTTTGCATCACCGAACTTCCCGCTTCTATTAGAGGCTGGCATTAAAAT
TCGCTGGCATGCAGGTAAACGCGCAAAGTTTGATCCAAAGCTTGAGCTTCAGGTCGCCAAAATTAGCCCGCAACCTATCG
GTGTTGTAACGCTTTATCCAGGGATCAACACAGAGATCTTTGAGAACATATTGCTTCAACCCGTTAAAGCACTCATCTTA
CTGACTTTTGGTGTCGGCAATGCACCACAAACACCCGAGCTGTTAAAAACCCTCAAGCAAGCTCATGAGCGCGGCATTGT
CATAGTTAACCTCACTCAGTGCTTGCAGGGGCGGGTTAATATGGATGGCTACGCCACAGGTAACGCACTTGCCGAAGCAG
GAGTTACCAGTGGTTACGATATGACAACAGAGGCTACACTGACTAAATTGCACTATTTATTGTCTACCGACATGACCCCT
GATGAGATCCGCGCTGCGATGAAGGTTTCTATTGCAGGTGAGTTAACCGCCGACTAG

Upstream 100 bases:

>100_bases
CTTACTTAGGATTATTGAGCCATATAGCCTGCATCTACTGTCACTTTGGGTATATAATCCATGCACCCTGCTTTAATCCA
GAACTAAAAAACAAAATCCA

Downstream 100 bases:

>100_bases
CCAAATAGTGTCAGGCCGTGACAGCATAAAAGAAAGCCCAAGCATTTTGCCTGGGCTTTTTTGTTAGCGATGTTTATCTT
ATACCAATCAGTATAAGAAG

Product: cytoplasmic asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MTKRSIYVAYTGGTIGMQKTDHGFAPVANFLTGCVKAMPEFYHDEMPDFVINEYSPLIDSANMKPTDWQMIANDIKAKYD
QYDGFVILHGTDTMAFTASALSFMLQGLQKPVIVTGSQIPLAQLRSDGQTNLLNALYIAANYPVAEVCLFFNNKLFRGNR
TTKVHADGFNAFASPNFPLLLEAGIKIRWHAGKRAKFDPKLELQVAKISPQPIGVVTLYPGINTEIFENILLQPVKALIL
LTFGVGNAPQTPELLKTLKQAHERGIVIVNLTQCLQGRVNMDGYATGNALAEAGVTSGYDMTTEATLTKLHYLLSTDMTP
DEIRAAMKVSIAGELTAD

Sequences:

>Translated_338_residues
MTKRSIYVAYTGGTIGMQKTDHGFAPVANFLTGCVKAMPEFYHDEMPDFVINEYSPLIDSANMKPTDWQMIANDIKAKYD
QYDGFVILHGTDTMAFTASALSFMLQGLQKPVIVTGSQIPLAQLRSDGQTNLLNALYIAANYPVAEVCLFFNNKLFRGNR
TTKVHADGFNAFASPNFPLLLEAGIKIRWHAGKRAKFDPKLELQVAKISPQPIGVVTLYPGINTEIFENILLQPVKALIL
LTFGVGNAPQTPELLKTLKQAHERGIVIVNLTQCLQGRVNMDGYATGNALAEAGVTSGYDMTTEATLTKLHYLLSTDMTP
DEIRAAMKVSIAGELTAD
>Mature_337_residues
TKRSIYVAYTGGTIGMQKTDHGFAPVANFLTGCVKAMPEFYHDEMPDFVINEYSPLIDSANMKPTDWQMIANDIKAKYDQ
YDGFVILHGTDTMAFTASALSFMLQGLQKPVIVTGSQIPLAQLRSDGQTNLLNALYIAANYPVAEVCLFFNNKLFRGNRT
TKVHADGFNAFASPNFPLLLEAGIKIRWHAGKRAKFDPKLELQVAKISPQPIGVVTLYPGINTEIFENILLQPVKALILL
TFGVGNAPQTPELLKTLKQAHERGIVIVNLTQCLQGRVNMDGYATGNALAEAGVTSGYDMTTEATLTKLHYLLSTDMTPD
EIRAAMKVSIAGELTAD

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=356, Percent_Identity=43.8202247191011, Blast_Score=265, Evalue=4e-71,
Organism=Escherichia coli, GI1788065, Length=338, Percent_Identity=67.1597633136095, Blast_Score=466, Evalue=1e-133,
Organism=Escherichia coli, GI1789327, Length=235, Percent_Identity=29.7872340425532, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=362, Percent_Identity=40.6077348066298, Blast_Score=267, Evalue=7e-72,
Organism=Caenorhabditis elegans, GI17558280, Length=357, Percent_Identity=41.1764705882353, Blast_Score=267, Evalue=7e-72,
Organism=Caenorhabditis elegans, GI17558278, Length=357, Percent_Identity=41.1764705882353, Blast_Score=266, Evalue=8e-72,
Organism=Caenorhabditis elegans, GI193207374, Length=362, Percent_Identity=40.6077348066298, Blast_Score=266, Evalue=9e-72,
Organism=Drosophila melanogaster, GI24639609, Length=362, Percent_Identity=40.6077348066298, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24645530, Length=368, Percent_Identity=40.7608695652174, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37028; Mature: 36897

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRSIYVAYTGGTIGMQKTDHGFAPVANFLTGCVKAMPEFYHDEMPDFVINEYSPLIDS
CCCCEEEEEEECCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHCC
ANMKPTDWQMIANDIKAKYDQYDGFVILHGTDTMAFTASALSFMLQGLQKPVIVTGSQIP
CCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
LAQLRSDGQTNLLNALYIAANYPVAEVCLFFNNKLFRGNRTTKVHADGFNAFASPNFPLL
HHHHHCCCCHHHEEEEEEECCCCHHHHHHHHCCEEECCCCCEEEECCCCCCCCCCCCCEE
LEAGIKIRWHAGKRAKFDPKLELQVAKISPQPIGVVTLYPGINTEIFENILLQPVKALIL
EECCEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHE
LTFGVGNAPQTPELLKTLKQAHERGIVIVNLTQCLQGRVNMDGYATGNALAEAGVTSGYD
EEECCCCCCCCHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCCEECCCHHHHCCCCCCCC
MTTEATLTKLHYLLSTDMTPDEIRAAMKVSIAGELTAD
CCHHHHHHHHHHHHHCCCCHHHHHHHHEEEEECEECCC
>Mature Secondary Structure 
TKRSIYVAYTGGTIGMQKTDHGFAPVANFLTGCVKAMPEFYHDEMPDFVINEYSPLIDS
CCCEEEEEEECCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHCC
ANMKPTDWQMIANDIKAKYDQYDGFVILHGTDTMAFTASALSFMLQGLQKPVIVTGSQIP
CCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
LAQLRSDGQTNLLNALYIAANYPVAEVCLFFNNKLFRGNRTTKVHADGFNAFASPNFPLL
HHHHHCCCCHHHEEEEEEECCCCHHHHHHHHCCEEECCCCCEEEECCCCCCCCCCCCCEE
LEAGIKIRWHAGKRAKFDPKLELQVAKISPQPIGVVTLYPGINTEIFENILLQPVKALIL
EECCEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHE
LTFGVGNAPQTPELLKTLKQAHERGIVIVNLTQCLQGRVNMDGYATGNALAEAGVTSGYD
EEECCCCCCCCHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCCEECCCHHHHCCCCCCCC
MTTEATLTKLHYLLSTDMTPDEIRAAMKVSIAGELTAD
CCHHHHHHHHHHHHHCCCCHHHHHHHHEEEEECEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]