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The map label for this gene is sppA [H]

Identifier: 157962074

GI number: 157962074

Start: 2732249

End: 2734090

Strand: Direct

Name: sppA [H]

Synonym: Spea_2253

Alternate gene names: 157962074

Gene position: 2732249-2734090 (Clockwise)

Preceding gene: 157962071

Following gene: 157962075

Centisome position: 52.8

GC content: 44.73

Gene sequence:

>1842_bases
ATGCCCACTAAACCCTCAATTTTTAAAAGGATATTCCTCCTTATTTGGAATACCGTTAATGGATTAAGAAAGCTTTTCCT
TAATCTTATCTTCTTCGGTGTCATTGCCCTTATCATCGTAAGTCTTAGCACCGACGATGGTGTAGAAGTTGAAAATGGTG
CCGCACTGGTGCTCAATCTATCTGGCACAATTGTTGACCAAAAACGTCAAGTCGATCCTATCGAAGCGGCGATGAAAAGT
GGCAATGAAGCCGATGGAAGCGGCGAAATTTTACTTTCTGATGTGCTATACGTGATAGATAACGCTGCTTCTGATGAACG
TATTAGTCAACTGGTATTAGATTTAGGCATGTTGCGCGGTACAGGTATAAGCAAGCTGCAATCTATTGGTAACGCGATTG
ATTCATTCAAGGCCACTGGCAAAACTGTTGTCGCCAATGGCAATTGGTATGGTCAAAATCACTATTTTCTTGCGAGCTTT
GCAGACAAAGTCTATCTAAACCCTCAAGGGAGTGTCGAAATTGAAGGTCTGGGCCGCTATCGTCTCTATTTTAAATCTGC
ATTAGAGAAGCTTAAGATCAATGCTCACGTGTTTAGAGTCGGTACTTTCAAGTCTGCGGTAGAACCTTTTATTCGTGATG
ATATGTCTGATGAAGCTAAAGAAGCTAACTTAGTACTATTAAACGATTTATGGCGAAGCTACGCTGACACAGTTGCGGCT
AACCGTGGCATTAATAGCAACGACTTATCCCTAAGTGCCGATGATTACCTCGCCCAGCTTGATAAAGCGGACGGTAAGTC
GGCTGATATGGCTGTTAATATGAAATGGGTCGATGGTCTTAAGTCTGCTGAAGAGTTTAGGCTTTCGATGGTGGATGCCG
TCGGCAAGTCTAGCGATGGTAACAGCTACAAACATATCGATTTTTATGATTATCTGTCTGTGACTCAAGCTCATCCTTCA
TTATCCTTAAACGATCAAGTTGGCATCATTGTCGCAAAGGGTAATATCTTAAATGGTTCACAGCCTGCCGGACAAATTGG
TGGCGACAGTACATCTGAGCTACTTCGAAAAGCCCGTTTCGATGATTCGATAAAAGCGGTTGTACTCAGAGTCGACAGCC
CAGGTGGTAGTGCCTTTGCTTCCGAGCAGATCAGACAAGAAGTCCTTGCACTTAAGGCGGCTAACAAGCCTGTCGTTGTC
AGCATGGGCAGCTACGCTGCATCGGGTGGATACTGGATCTCTGCCAGTGCAGACTATATTTATGCAACGCCAACAACGCT
CACTGGCTCAATTGGGATCTTCGGTATGATCACTACCTTTGAAGACTCATTAGCAAGCATTGGGGTTCATACAGATGGTG
TCGGTACTTCTGAGTGGGCAGGCTTCTCTGTGACTAAAGGCCTTTCACCTCAGATCCAAGCTGTCATTCAGCGCCATATT
GAACGTGGATACTACGACTTTATCTCGTTAGTCGCTAAAGAGCGTGATATGAGCTTAGATCAGGTTGATAGCATCGCTCA
GGGTCGAGTGTGGACCGGTCGTAAGGCATTAGAGCTAGGACTCGTCGATGGTTTAGGTGAGTTGCAAGATGCGGTAACCA
AGGCTGCTGAAATGGCTTCTCTCGATACCTTTGATACCGAGGTGATTGAGAGAGAGTTGTCTCCACAAGAGCAATTCATT
CAAGAGATGTTCGCTACGGCTTCGAGCCATCTACCACCGAGCGTCACTCAATCGAGCCTACTTGAAACCATCCTTAGTCA
ATGGTCTGGTGTGGTTGAAGAGTTTAAAGCTTTCGATGATCCAAATGGTGTTTATCTTTATTGCGATACCTGTAACTTTT
AA

Upstream 100 bases:

>100_bases
ACCTCAATAAGTGTATGAATAGATTCCTTTACAATTGAGTTTTCCTTTCATGACGGTTTTCAGTTAGCATTATGCCAACT
AAGCTAGAAAGGAGTGGCTA

Downstream 100 bases:

>100_bases
TTACTAATTAAGGTTACAGTTAACAAAATATGCCCGGCAAATGCTGGGCATATTTATTTTCAAATCCACTCTCCTACACC
AAATGCTACCAACCACGCTA

Product: signal peptide peptidase SppA

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MPTKPSIFKRIFLLIWNTVNGLRKLFLNLIFFGVIALIIVSLSTDDGVEVENGAALVLNLSGTIVDQKRQVDPIEAAMKS
GNEADGSGEILLSDVLYVIDNAASDERISQLVLDLGMLRGTGISKLQSIGNAIDSFKATGKTVVANGNWYGQNHYFLASF
ADKVYLNPQGSVEIEGLGRYRLYFKSALEKLKINAHVFRVGTFKSAVEPFIRDDMSDEAKEANLVLLNDLWRSYADTVAA
NRGINSNDLSLSADDYLAQLDKADGKSADMAVNMKWVDGLKSAEEFRLSMVDAVGKSSDGNSYKHIDFYDYLSVTQAHPS
LSLNDQVGIIVAKGNILNGSQPAGQIGGDSTSELLRKARFDDSIKAVVLRVDSPGGSAFASEQIRQEVLALKAANKPVVV
SMGSYAASGGYWISASADYIYATPTTLTGSIGIFGMITTFEDSLASIGVHTDGVGTSEWAGFSVTKGLSPQIQAVIQRHI
ERGYYDFISLVAKERDMSLDQVDSIAQGRVWTGRKALELGLVDGLGELQDAVTKAAEMASLDTFDTEVIERELSPQEQFI
QEMFATASSHLPPSVTQSSLLETILSQWSGVVEEFKAFDDPNGVYLYCDTCNF

Sequences:

>Translated_613_residues
MPTKPSIFKRIFLLIWNTVNGLRKLFLNLIFFGVIALIIVSLSTDDGVEVENGAALVLNLSGTIVDQKRQVDPIEAAMKS
GNEADGSGEILLSDVLYVIDNAASDERISQLVLDLGMLRGTGISKLQSIGNAIDSFKATGKTVVANGNWYGQNHYFLASF
ADKVYLNPQGSVEIEGLGRYRLYFKSALEKLKINAHVFRVGTFKSAVEPFIRDDMSDEAKEANLVLLNDLWRSYADTVAA
NRGINSNDLSLSADDYLAQLDKADGKSADMAVNMKWVDGLKSAEEFRLSMVDAVGKSSDGNSYKHIDFYDYLSVTQAHPS
LSLNDQVGIIVAKGNILNGSQPAGQIGGDSTSELLRKARFDDSIKAVVLRVDSPGGSAFASEQIRQEVLALKAANKPVVV
SMGSYAASGGYWISASADYIYATPTTLTGSIGIFGMITTFEDSLASIGVHTDGVGTSEWAGFSVTKGLSPQIQAVIQRHI
ERGYYDFISLVAKERDMSLDQVDSIAQGRVWTGRKALELGLVDGLGELQDAVTKAAEMASLDTFDTEVIERELSPQEQFI
QEMFATASSHLPPSVTQSSLLETILSQWSGVVEEFKAFDDPNGVYLYCDTCNF
>Mature_612_residues
PTKPSIFKRIFLLIWNTVNGLRKLFLNLIFFGVIALIIVSLSTDDGVEVENGAALVLNLSGTIVDQKRQVDPIEAAMKSG
NEADGSGEILLSDVLYVIDNAASDERISQLVLDLGMLRGTGISKLQSIGNAIDSFKATGKTVVANGNWYGQNHYFLASFA
DKVYLNPQGSVEIEGLGRYRLYFKSALEKLKINAHVFRVGTFKSAVEPFIRDDMSDEAKEANLVLLNDLWRSYADTVAAN
RGINSNDLSLSADDYLAQLDKADGKSADMAVNMKWVDGLKSAEEFRLSMVDAVGKSSDGNSYKHIDFYDYLSVTQAHPSL
SLNDQVGIIVAKGNILNGSQPAGQIGGDSTSELLRKARFDDSIKAVVLRVDSPGGSAFASEQIRQEVLALKAANKPVVVS
MGSYAASGGYWISASADYIYATPTTLTGSIGIFGMITTFEDSLASIGVHTDGVGTSEWAGFSVTKGLSPQIQAVIQRHIE
RGYYDFISLVAKERDMSLDQVDSIAQGRVWTGRKALELGLVDGLGELQDAVTKAAEMASLDTFDTEVIERELSPQEQFIQ
EMFATASSHLPPSVTQSSLLETILSQWSGVVEEFKAFDDPNGVYLYCDTCNF

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=609, Percent_Identity=43.6781609195402, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1787527, Length=163, Percent_Identity=32.5153374233129, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 66515; Mature: 66383

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTKPSIFKRIFLLIWNTVNGLRKLFLNLIFFGVIALIIVSLSTDDGVEVENGAALVLNL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEECCCCEEEEEE
SGTIVDQKRQVDPIEAAMKSGNEADGSGEILLSDVLYVIDNAASDERISQLVLDLGMLRG
CCCEECCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
TGISKLQSIGNAIDSFKATGKTVVANGNWYGQNHYFLASFADKVYLNPQGSVEIEGLGRY
CCHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCEEEEECCCEEEECCCCCEEEECCCHH
RLYFKSALEKLKINAHVFRVGTFKSAVEPFIRDDMSDEAKEANLVLLNDLWRSYADTVAA
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCHHHCCEEEHHHHHHHHHHHHHH
NRGINSNDLSLSADDYLAQLDKADGKSADMAVNMKWVDGLKSAEEFRLSMVDAVGKSSDG
CCCCCCCCCEECHHHHHHHHHCCCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHCCCCCC
NSYKHIDFYDYLSVTQAHPSLSLNDQVGIIVAKGNILNGSQPAGQIGGDSTSELLRKARF
CCEEEEEHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCC
DDSIKAVVLRVDSPGGSAFASEQIRQEVLALKAANKPVVVSMGSYAASGGYWISASADYI
CCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCEECCCCEEEEECCCEE
YATPTTLTGSIGIFGMITTFEDSLASIGVHTDGVGTSEWAGFSVTKGLSPQIQAVIQRHI
EECCCEEECCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHH
ERGYYDFISLVAKERDMSLDQVDSIAQGRVWTGRKALELGLVDGLGELQDAVTKAAEMAS
HCCHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCHHHEEHHHCCHHHHHHHHHHHHHHHH
LDTFDTEVIERELSPQEQFIQEMFATASSHLPPSVTQSSLLETILSQWSGVVEEFKAFDD
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
PNGVYLYCDTCNF
CCCEEEEEECCCC
>Mature Secondary Structure 
PTKPSIFKRIFLLIWNTVNGLRKLFLNLIFFGVIALIIVSLSTDDGVEVENGAALVLNL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEECCCCEEEEEE
SGTIVDQKRQVDPIEAAMKSGNEADGSGEILLSDVLYVIDNAASDERISQLVLDLGMLRG
CCCEECCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
TGISKLQSIGNAIDSFKATGKTVVANGNWYGQNHYFLASFADKVYLNPQGSVEIEGLGRY
CCHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCEEEEECCCEEEECCCCCEEEECCCHH
RLYFKSALEKLKINAHVFRVGTFKSAVEPFIRDDMSDEAKEANLVLLNDLWRSYADTVAA
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCHHHCCEEEHHHHHHHHHHHHHH
NRGINSNDLSLSADDYLAQLDKADGKSADMAVNMKWVDGLKSAEEFRLSMVDAVGKSSDG
CCCCCCCCCEECHHHHHHHHHCCCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHCCCCCC
NSYKHIDFYDYLSVTQAHPSLSLNDQVGIIVAKGNILNGSQPAGQIGGDSTSELLRKARF
CCEEEEEHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCC
DDSIKAVVLRVDSPGGSAFASEQIRQEVLALKAANKPVVVSMGSYAASGGYWISASADYI
CCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCEECCCCEEEEECCCEE
YATPTTLTGSIGIFGMITTFEDSLASIGVHTDGVGTSEWAGFSVTKGLSPQIQAVIQRHI
EECCCEEECCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHH
ERGYYDFISLVAKERDMSLDQVDSIAQGRVWTGRKALELGLVDGLGELQDAVTKAAEMAS
HCCHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCHHHEEHHHCCHHHHHHHHHHHHHHHH
LDTFDTEVIERELSPQEQFIQEMFATASSHLPPSVTQSSLLETILSQWSGVVEEFKAFDD
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
PNGVYLYCDTCNF
CCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3522590; 9097039; 9278503; 7973728 [H]