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The map label for this gene is cls [H]

Identifier: 157962061

GI number: 157962061

Start: 2715271

End: 2716728

Strand: Direct

Name: cls [H]

Synonym: Spea_2240

Alternate gene names: 157962061

Gene position: 2715271-2716728 (Clockwise)

Preceding gene: 157962056

Following gene: 157962062

Centisome position: 52.47

GC content: 44.51

Gene sequence:

>1458_bases
ATGTATGAATTTTATCAGCTACTCACCATAGCTGGCGTGTTTCTTTACTGGCTTATTATTGCAGGTATCAGTATTCGCGT
AGTTGCAAAGCGGCGCTCTATTGGAGTTTCTTTAGCCTGGTTAATGATTATTTATATCGTGCCATTAGGTGGCGCCTTTG
CCTATCTACTGTTTGGTGAATTAAATTTAGGTAAGAAGAGAGCGGTTCGCGCAGAGCTGATGTTTAAACCCTACGGTGAA
TGGTTTAAAAAACTCTATGAGTATCGGCAATATCGCCCTCATGTCGCGAGTCTATACGGCCAGTCGATCAGTGCCATTTG
TGAAAAACAAGTTGGGATCCCTGCACTTGCCGATAACCATCTGACTCTGTGTAGTGAAACTTATGAGATCTTAGATAACT
TAATTGAAGATATCGACAAGGCAAGTGAGACGATTCTACTCGAGTTCTATATCTGGCACCCCGGTGGGCGCGCCGATGAT
GTAGCTAACGCCCTCATCAATAGCGCTGCTCGAGGGGTGAAAGTACGCTTATTACTTGACGCTGCGGGCAGCAGGCACTT
CTTCAAGAGCCATTGGCCAAAATTAATGAAAAAAAGTGGCATTCAGGTGATCAGTGCTCTCGAAGTGTCTCCTTTTCGTA
TGTTCTTTAGACGGCTAGATCTTAGACTTCATAGAAAGATCGTCGTCATCGATAATCAAATAGGTTATACCGGCTCAATG
AATCTTGTCGACCCTAAGTATTTTAATCAAGATGCTGGCGTTGGCGAATGGATTGATGTGATGGTGCGGATCACCGGCAC
CTCAGTTCCAGTGCTTAATAGCATCCATGCCTGGGATTGGGAAGTGGAAACTAATGAGCGTTTCTTGCCTGAACAGCCAA
AATGTTTGTCTCATGAGGACAGCGAGATCACAGACTTAGTTCAGGTGATCCCATCTGGGCCAGGTATGCGTGAAGAGGTG
ATCCATCGAGTGCTACTACAAAGCATTTACCAGGCCTCAAAAAGTATCGTTATCACTACGCCCTACTTTGTGCCAAGTGA
CAATCTACATATGGCACTGATCATTGCGGCGCAACGTAACGTTAAGGTCGACATTATCGTTCCTGATAAGAATGATTCAT
TAATGGTTGAATGGGCGAGTCGAGCATTTTTTAGTGAACTGTTAGAGGCGGGCGTTAATATTCATCGATTTAAAGGCGGT
CTATTGCACACCAAATCTGTTGTCATCGACAGTAATCATTGCTTGATTGGCACAGTTAACTGGGACATGCGCAGTTTATG
GCTCAACTTCGAAGTGACCCTTGCGGTTGATAACCTAGGCTTTACCAAAAAGCTTGAACAGGTACAGCAAGAGTATCTTA
AAAATGCCGATAAGGTCTGCTTCGAGCAATGGCAAAAGCGTTCAATAGCCAGTCGCGGAGCCGAACGAATTTTCTATTTA
TTCAGCCCGTTACTCTAG

Upstream 100 bases:

>100_bases
TAGTGTTAGTCACATAATCATTCAATTAAGAGGTTAGTTAGCTCAAATCATCGTTTATCCGCTGATTTATGCGACTAAAG
GTAAGACTATCAGACACAAT

Downstream 100 bases:

>100_bases
TTCTTGCAATGATTCTTATATTCGCTGTTTTCTTTAGCCGCCAACGTAGCGATTCGTTGGCGCCACTATGGCCACAAAGC
ATGACTCTAATTGGATTGTT

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MYEFYQLLTIAGVFLYWLIIAGISIRVVAKRRSIGVSLAWLMIIYIVPLGGAFAYLLFGELNLGKKRAVRAELMFKPYGE
WFKKLYEYRQYRPHVASLYGQSISAICEKQVGIPALADNHLTLCSETYEILDNLIEDIDKASETILLEFYIWHPGGRADD
VANALINSAARGVKVRLLLDAAGSRHFFKSHWPKLMKKSGIQVISALEVSPFRMFFRRLDLRLHRKIVVIDNQIGYTGSM
NLVDPKYFNQDAGVGEWIDVMVRITGTSVPVLNSIHAWDWEVETNERFLPEQPKCLSHEDSEITDLVQVIPSGPGMREEV
IHRVLLQSIYQASKSIVITTPYFVPSDNLHMALIIAAQRNVKVDIIVPDKNDSLMVEWASRAFFSELLEAGVNIHRFKGG
LLHTKSVVIDSNHCLIGTVNWDMRSLWLNFEVTLAVDNLGFTKKLEQVQQEYLKNADKVCFEQWQKRSIASRGAERIFYL
FSPLL

Sequences:

>Translated_485_residues
MYEFYQLLTIAGVFLYWLIIAGISIRVVAKRRSIGVSLAWLMIIYIVPLGGAFAYLLFGELNLGKKRAVRAELMFKPYGE
WFKKLYEYRQYRPHVASLYGQSISAICEKQVGIPALADNHLTLCSETYEILDNLIEDIDKASETILLEFYIWHPGGRADD
VANALINSAARGVKVRLLLDAAGSRHFFKSHWPKLMKKSGIQVISALEVSPFRMFFRRLDLRLHRKIVVIDNQIGYTGSM
NLVDPKYFNQDAGVGEWIDVMVRITGTSVPVLNSIHAWDWEVETNERFLPEQPKCLSHEDSEITDLVQVIPSGPGMREEV
IHRVLLQSIYQASKSIVITTPYFVPSDNLHMALIIAAQRNVKVDIIVPDKNDSLMVEWASRAFFSELLEAGVNIHRFKGG
LLHTKSVVIDSNHCLIGTVNWDMRSLWLNFEVTLAVDNLGFTKKLEQVQQEYLKNADKVCFEQWQKRSIASRGAERIFYL
FSPLL
>Mature_485_residues
MYEFYQLLTIAGVFLYWLIIAGISIRVVAKRRSIGVSLAWLMIIYIVPLGGAFAYLLFGELNLGKKRAVRAELMFKPYGE
WFKKLYEYRQYRPHVASLYGQSISAICEKQVGIPALADNHLTLCSETYEILDNLIEDIDKASETILLEFYIWHPGGRADD
VANALINSAARGVKVRLLLDAAGSRHFFKSHWPKLMKKSGIQVISALEVSPFRMFFRRLDLRLHRKIVVIDNQIGYTGSM
NLVDPKYFNQDAGVGEWIDVMVRITGTSVPVLNSIHAWDWEVETNERFLPEQPKCLSHEDSEITDLVQVIPSGPGMREEV
IHRVLLQSIYQASKSIVITTPYFVPSDNLHMALIIAAQRNVKVDIIVPDKNDSLMVEWASRAFFSELLEAGVNIHRFKGG
LLHTKSVVIDSNHCLIGTVNWDMRSLWLNFEVTLAVDNLGFTKKLEQVQQEYLKNADKVCFEQWQKRSIASRGAERIFYL
FSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=487, Percent_Identity=48.870636550308, Blast_Score=493, Evalue=1e-140,
Organism=Escherichia coli, GI1787007, Length=337, Percent_Identity=26.4094955489614, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 55375; Mature: 55375

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEFYQLLTIAGVFLYWLIIAGISIRVVAKRRSIGVSLAWLMIIYIVPLGGAFAYLLFGE
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHCCCHHHHHHHHHHHHCCCHHHHHHHHHC
LNLGKKRAVRAELMFKPYGEWFKKLYEYRQYRPHVASLYGQSISAICEKQVGIPALADNH
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEECCCC
LTLCSETYEILDNLIEDIDKASETILLEFYIWHPGGRADDVANALINSAARGVKVRLLLD
CHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEE
AAGSRHFFKSHWPKLMKKSGIQVISALEVSPFRMFFRRLDLRLHRKIVVIDNQIGYTGSM
CCCCCHHHHHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
NLVDPKYFNQDAGVGEWIDVMVRITGTSVPVLNSIHAWDWEVETNERFLPEQPKCLSHED
CCCCCHHCCCCCCCCHHEEEEEEECCCCCCHHCCCCCCCEEECCCCCCCCCCCCCCCCCC
SEITDLVQVIPSGPGMREEVIHRVLLQSIYQASKSIVITTPYFVPSDNLHMALIIAAQRN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECEEECCCCCEEEEEEEECCC
VKVDIIVPDKNDSLMVEWASRAFFSELLEAGVNIHRFKGGLLHTKSVVIDSNHCLIGTVN
EEEEEEEECCCCCEEEHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEEEECCCEEEEEEC
WDMRSLWLNFEVTLAVDNLGFTKKLEQVQQEYLKNADKVCFEQWQKRSIASRGAERIFYL
CCEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
FSPLL
HHCCC
>Mature Secondary Structure
MYEFYQLLTIAGVFLYWLIIAGISIRVVAKRRSIGVSLAWLMIIYIVPLGGAFAYLLFGE
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHCCCHHHHHHHHHHHHCCCHHHHHHHHHC
LNLGKKRAVRAELMFKPYGEWFKKLYEYRQYRPHVASLYGQSISAICEKQVGIPALADNH
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEECCCC
LTLCSETYEILDNLIEDIDKASETILLEFYIWHPGGRADDVANALINSAARGVKVRLLLD
CHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEE
AAGSRHFFKSHWPKLMKKSGIQVISALEVSPFRMFFRRLDLRLHRKIVVIDNQIGYTGSM
CCCCCHHHHHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
NLVDPKYFNQDAGVGEWIDVMVRITGTSVPVLNSIHAWDWEVETNERFLPEQPKCLSHED
CCCCCHHCCCCCCCCHHEEEEEEECCCCCCHHCCCCCCCEEECCCCCCCCCCCCCCCCCC
SEITDLVQVIPSGPGMREEVIHRVLLQSIYQASKSIVITTPYFVPSDNLHMALIIAAQRN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECEEECCCCCEEEEEEEECCC
VKVDIIVPDKNDSLMVEWASRAFFSELLEAGVNIHRFKGGLLHTKSVVIDSNHCLIGTVN
EEEEEEEECCCCCEEEHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEEEECCCEEEEEEC
WDMRSLWLNFEVTLAVDNLGFTKKLEQVQQEYLKNADKVCFEQWQKRSIASRGAERIFYL
CCEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
FSPLL
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA