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The map label for this gene is ltaA [H]

Identifier: 157962026

GI number: 157962026

Start: 2674405

End: 2675418

Strand: Direct

Name: ltaA [H]

Synonym: Spea_2205

Alternate gene names: 157962026

Gene position: 2674405-2675418 (Clockwise)

Preceding gene: 157962025

Following gene: 157962027

Centisome position: 51.68

GC content: 46.65

Gene sequence:

>1014_bases
ATGGCAGGAGTAATCGATTTACGCAGTGATACCGTCACGCAGCCTTGCCAGAACATGCGCGCGTTAATGGCATCAGCCGA
AACGGGTGATGATGTTTATGGTGAAGATCCTAGCGTTAAGCAGCTTGAATCCTATGCAGCTGAATTACTGAATAAACAAG
CTGCGGTATTTTGCCCCTCAGGCACGCAAAGCAACTTAATGGGGCTGCTGTCTCATTGTGGCCGAGGCGATGAGTACATT
GTTGGCAACGTTGCACATACCTATCTTTACGAAGGCGGCGGCGCCGCGGTATTAGGGAGTATTCAGCCACAGCCATTAAC
GCTACAAGCTGATGCAACCATGGATCTTTCCGAGCTGGAAAAAGCCATTAAGCCTAAAGACGTTCACTATGCCAGAACCA
AGTTGATATGCCTGGAAAACACTCATGGAGGTCTTCCTTTACCTGACGGATACACTCAAAACGTTAAAGCGATAGCACAG
CGCCATGGCTTAAGCATGCATTTAGATGGTGCTAGATTATTTAATGCCGTTGTTGCTGGTAATAAGAGTGCTGCGACATT
AACTGCAGATTTTGACAGTGTTTCAATCTGTTTATCAAAAGGCTTAGGAGCGCCTGTTGGCTCGTTACTCGTAGGCTCAA
ATAAGTTTATCGATGAAGCTAGGCACTGGCGAAAAATGTTAGGTGGCGGTATGCGCCAAGCAGGAATTATTGCCGCTGCA
GGCTTGTATGCCTTACAAAACAACGTTAGTCGCTTGAGCGATGATCACCGCCGAGCGAGCGAATTAGCAAATGCGCTAGG
GCAGATTGACGGTGTTAGTATCCCGTTAGGTCAGGCCAACACCAACATGTTATATGTGAGCGTTAGCCAAGCAATGAGAG
AACGCCTTGCTGAGCGTGCAGCCAATTACAATATATTGCTACCTGCTGGTGAGCAGATGCGTTTGGTCACTCACTTAAAT
ATTAATGATGATGACCTAAAAGCCATTATTGAGTTATTTCAACAAGCGGCTTAA

Upstream 100 bases:

>100_bases
ATGCGCGTAGCGCTTTTAGCGGCTTCCGACATCTTGCCGGCGGCTACACTAATCGAGAAGATTACATAAGTAAAAAGCTA
ACCTTAAAAGGGAAAACACC

Downstream 100 bases:

>100_bases
CGCTAGGGCACCTGATGACACAAAACATCTCTTCACATTCCCAAGGCCATAGAGTCATGGGGTTTTGGCGCGTTTGGGCG
CTTGCCGTAGGATGTGCTAT

Product: threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MAGVIDLRSDTVTQPCQNMRALMASAETGDDVYGEDPSVKQLESYAAELLNKQAAVFCPSGTQSNLMGLLSHCGRGDEYI
VGNVAHTYLYEGGGAAVLGSIQPQPLTLQADATMDLSELEKAIKPKDVHYARTKLICLENTHGGLPLPDGYTQNVKAIAQ
RHGLSMHLDGARLFNAVVAGNKSAATLTADFDSVSICLSKGLGAPVGSLLVGSNKFIDEARHWRKMLGGGMRQAGIIAAA
GLYALQNNVSRLSDDHRRASELANALGQIDGVSIPLGQANTNMLYVSVSQAMRERLAERAANYNILLPAGEQMRLVTHLN
INDDDLKAIIELFQQAA

Sequences:

>Translated_337_residues
MAGVIDLRSDTVTQPCQNMRALMASAETGDDVYGEDPSVKQLESYAAELLNKQAAVFCPSGTQSNLMGLLSHCGRGDEYI
VGNVAHTYLYEGGGAAVLGSIQPQPLTLQADATMDLSELEKAIKPKDVHYARTKLICLENTHGGLPLPDGYTQNVKAIAQ
RHGLSMHLDGARLFNAVVAGNKSAATLTADFDSVSICLSKGLGAPVGSLLVGSNKFIDEARHWRKMLGGGMRQAGIIAAA
GLYALQNNVSRLSDDHRRASELANALGQIDGVSIPLGQANTNMLYVSVSQAMRERLAERAANYNILLPAGEQMRLVTHLN
INDDDLKAIIELFQQAA
>Mature_336_residues
AGVIDLRSDTVTQPCQNMRALMASAETGDDVYGEDPSVKQLESYAAELLNKQAAVFCPSGTQSNLMGLLSHCGRGDEYIV
GNVAHTYLYEGGGAAVLGSIQPQPLTLQADATMDLSELEKAIKPKDVHYARTKLICLENTHGGLPLPDGYTQNVKAIAQR
HGLSMHLDGARLFNAVVAGNKSAATLTADFDSVSICLSKGLGAPVGSLLVGSNKFIDEARHWRKMLGGGMRQAGIIAAAG
LYALQNNVSRLSDDHRRASELANALGQIDGVSIPLGQANTNMLYVSVSQAMRERLAERAANYNILLPAGEQMRLVTHLNI
NDDDLKAIIELFQQAA

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=326, Percent_Identity=52.4539877300613, Blast_Score=343, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI193206522, Length=342, Percent_Identity=42.3976608187134, Blast_Score=262, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI193206520, Length=342, Percent_Identity=42.3976608187134, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI193206524, Length=342, Percent_Identity=42.3976608187134, Blast_Score=262, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6320789, Length=342, Percent_Identity=36.2573099415205, Blast_Score=186, Evalue=3e-48,
Organism=Drosophila melanogaster, GI21355439, Length=300, Percent_Identity=45.6666666666667, Blast_Score=265, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 35987; Mature: 35856

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVIDLRSDTVTQPCQNMRALMASAETGDDVYGEDPSVKQLESYAAELLNKQAAVFCPS
CCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECC
GTQSNLMGLLSHCGRGDEYIVGNVAHTYLYEGGGAAVLGSIQPQPLTLQADATMDLSELE
CCHHHHHHHHHHCCCCCCEEECCEEEEEEEECCCEEEECCCCCCCEEEECCCCCCHHHHH
KAIKPKDVHYARTKLICLENTHGGLPLPDGYTQNVKAIAQRHGLSMHLDGARLFNAVVAG
HHCCCCCCEEEHEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHCC
NKSAATLTADFDSVSICLSKGLGAPVGSLLVGSNKFIDEARHWRKMLGGGMRQAGIIAAA
CCCCEEEEECCCHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
GLYALQNNVSRLSDDHRRASELANALGQIDGVSIPLGQANTNMLYVSVSQAMRERLAERA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEHHHHHHHHHHHHH
ANYNILLPAGEQMRLVTHLNINDDDLKAIIELFQQAA
CCCEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AGVIDLRSDTVTQPCQNMRALMASAETGDDVYGEDPSVKQLESYAAELLNKQAAVFCPS
CCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECC
GTQSNLMGLLSHCGRGDEYIVGNVAHTYLYEGGGAAVLGSIQPQPLTLQADATMDLSELE
CCHHHHHHHHHHCCCCCCEEECCEEEEEEEECCCEEEECCCCCCCEEEECCCCCCHHHHH
KAIKPKDVHYARTKLICLENTHGGLPLPDGYTQNVKAIAQRHGLSMHLDGARLFNAVVAG
HHCCCCCCEEEHEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHCC
NKSAATLTADFDSVSICLSKGLGAPVGSLLVGSNKFIDEARHWRKMLGGGMRQAGIIAAA
CCCCEEEEECCCHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
GLYALQNNVSRLSDDHRRASELANALGQIDGVSIPLGQANTNMLYVSVSQAMRERLAERA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEHHHHHHHHHHHHH
ANYNILLPAGEQMRLVTHLNINDDDLKAIIELFQQAA
CCCEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]