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The map label for this gene is nfsA [H]

Identifier: 157961073

GI number: 157961073

Start: 1518901

End: 1519629

Strand: Direct

Name: nfsA [H]

Synonym: Spea_1245

Alternate gene names: 157961073

Gene position: 1518901-1519629 (Clockwise)

Preceding gene: 157961071

Following gene: 157961074

Centisome position: 29.35

GC content: 48.29

Gene sequence:

>729_bases
ATGACCATGAATCACACCATAGAAACCATTTTACAGCATCGTTCAATTAGGCACTTTACCGAGCAGCAGATCGAAGCTGA
AAAATTGAAACTCATCTTACGCTGTGCCAATGCTGCCTCTAGCTCCAGTTTTATACAGTGTAGCAGTATTATCAGGGTTA
CTTTGCCAAGCGTCAGGGCCAAGCTGGCTGAGCTTGCGGGAGGGCAAGCCTATGTCGAGTCGGCAGCTGAGTTTCTGGTG
TTTTGCGCCGACTTTAACCGTCATCAGCAAATCCACCCAGCAGCCCAACTAGGTTTTACTGAGCAAACATTAATCGGTGC
AGTCGATACCGCTTTAATGGGGCAAAATGCCATGTTAGCGGCTGAGTCTATGGGGCTCGGTGGTGTGTATATTGGCGGGA
TCCGCAATGACCCTAAGCTGGTCTCAGAGCTCCTCGCTTTACCTAGGCATGTATTGCCATTATTTGGTATGTGCCTTGGC
TATCCGGCTAAAATACCGCAGCCAAAGCCACGCTTACCACTGAGTATCGTGGTCCACCAAGAAAGTTATCAAAGCTTAGA
TAATGCTGAACTTGAGAAGTACGATCAGCATATACAAGCCTATTACGCCAGCAGAAGCAGTAACAACAAGCAGACCTCTT
GGTCCGATCAAATTAGGGCGACCCTGAGTAAGGAGTCAAGACCCTTTATGCAGGCCTATCTAAACGGGCAGGGGTTTAGC
ACCAAGTGA

Upstream 100 bases:

>100_bases
AAAATGGATTTTACAATAGGAAAGAGGTTTCTTTTTAGTGCTTTAGCTTGCGACTTGCATGAAGGAAAAGTAGGCTATTA
TTTCAAATGTTATAACTGAA

Downstream 100 bases:

>100_bases
GTCGTTTGATAGGGTAAAAATATACCGCTCTGTATGCCATCGTTATTGTGCAGCTTTATCTCAAATTTTATGCATGTGTT
TATATGGATGTTTACATTTA

Product: nitroreductase A

Products: NA

Alternate protein names: Modulator of drug activity A [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MTMNHTIETILQHRSIRHFTEQQIEAEKLKLILRCANAASSSSFIQCSSIIRVTLPSVRAKLAELAGGQAYVESAAEFLV
FCADFNRHQQIHPAAQLGFTEQTLIGAVDTALMGQNAMLAAESMGLGGVYIGGIRNDPKLVSELLALPRHVLPLFGMCLG
YPAKIPQPKPRLPLSIVVHQESYQSLDNAELEKYDQHIQAYYASRSSNNKQTSWSDQIRATLSKESRPFMQAYLNGQGFS
TK

Sequences:

>Translated_242_residues
MTMNHTIETILQHRSIRHFTEQQIEAEKLKLILRCANAASSSSFIQCSSIIRVTLPSVRAKLAELAGGQAYVESAAEFLV
FCADFNRHQQIHPAAQLGFTEQTLIGAVDTALMGQNAMLAAESMGLGGVYIGGIRNDPKLVSELLALPRHVLPLFGMCLG
YPAKIPQPKPRLPLSIVVHQESYQSLDNAELEKYDQHIQAYYASRSSNNKQTSWSDQIRATLSKESRPFMQAYLNGQGFS
TK
>Mature_241_residues
TMNHTIETILQHRSIRHFTEQQIEAEKLKLILRCANAASSSSFIQCSSIIRVTLPSVRAKLAELAGGQAYVESAAEFLVF
CADFNRHQQIHPAAQLGFTEQTLIGAVDTALMGQNAMLAAESMGLGGVYIGGIRNDPKLVSELLALPRHVLPLFGMCLGY
PAKIPQPKPRLPLSIVVHQESYQSLDNAELEKYDQHIQAYYASRSSNNKQTSWSDQIRATLSKESRPFMQAYLNGQGFST
K

Specific function: Reduction of nitroaromatic compounds using NADH. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major component of the oxygen- insensitive nitroreductase activity in E.coli [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=240, Percent_Identity=59.1666666666667, Blast_Score=283, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26794; Mature: 26663

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMNHTIETILQHRSIRHFTEQQIEAEKLKLILRCANAASSSSFIQCSSIIRVTLPSVRA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
KLAELAGGQAYVESAAEFLVFCADFNRHQQIHPAAQLGFTEQTLIGAVDTALMGQNAMLA
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHH
AESMGLGGVYIGGIRNDPKLVSELLALPRHVLPLFGMCLGYPAKIPQPKPRLPLSIVVHQ
HHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
ESYQSLDNAELEKYDQHIQAYYASRSSNNKQTSWSDQIRATLSKESRPFMQAYLNGQGFS
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCC
TK
CC
>Mature Secondary Structure 
TMNHTIETILQHRSIRHFTEQQIEAEKLKLILRCANAASSSSFIQCSSIIRVTLPSVRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
KLAELAGGQAYVESAAEFLVFCADFNRHQQIHPAAQLGFTEQTLIGAVDTALMGQNAMLA
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHH
AESMGLGGVYIGGIRNDPKLVSELLALPRHVLPLFGMCLGYPAKIPQPKPRLPLSIVVHQ
HHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
ESYQSLDNAELEKYDQHIQAYYASRSSNNKQTSWSDQIRATLSKESRPFMQAYLNGQGFS
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCC
TK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7568050; 8755878; 8905232; 9278503; 2570347; 9440535; 11034992 [H]