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The map label for this gene is ykoW [H]

Identifier: 157961071

GI number: 157961071

Start: 1516340

End: 1517866

Strand: Direct

Name: ykoW [H]

Synonym: Spea_1243

Alternate gene names: 157961071

Gene position: 1516340-1517866 (Clockwise)

Preceding gene: 157961070

Following gene: 157961073

Centisome position: 29.3

GC content: 40.67

Gene sequence:

>1527_bases
ATGTTAAGCCATGTCTTAAACCAACGACATTCTTCTTGGCTTGTCTTTGCCTTGTTAATGCTGTGCCTGATCGTTTCTTA
TATTCTGACTCAATATCAGCTATACAAAAAGCTTGAAGTCAGGGTTAATGACTTTGATAACTTTTATAGCGAGCGCCTAG
AAAGTGCTGATCGAAGTTTACTTGCCGTAGAACAGGTGTTGAAAAATATAGAGGTGGGTAGATGCTCACAAGGGGTGGTA
ACGTCTCTTAAAGACTATATCTTAACTTCAGATGCCTTAGCTATAGTTTGGGTAAATTTTATCGATGAGCCAATTCTCTG
TTCGGCGCTAGGGGTTTCCCAATTACCAGATCCCAAATATTATCCCGTGATTGCTTCTATGGGAAATGGGTTAAAGATCT
ATCAAAAGGTGGATAGCGATAACCCAGATGAAAGAAAACCTGTTTATGTGGGACGGCATACGGAGCAATATCGCTGGTTT
ATCGGAATTGATGTTTTGTCCTATATACAAGAAATATTAAAAAATTGTGAGGACTGTGGGGCATTAAGTATTCAGTTTAA
TAGTAAGCATGAGCTATTTAGGAAAAACAAAAGTGCTCCATACTGGGGAGCTATGCAATTTAACTCAGAAGTCACGGGGT
TGACCTACAGGTTCTCCCTTAATTTATATAGCAAGGCTTTACTGTGGTTAACTCACTTTGTCGGTGTCATTGCTATCTCA
AGTATGTTTTTTGTTGGGTGGTTGTTACTTAGAGGTGTGTCAAGGCAATATTATTGGCACCGTCTGTTTTTAAAGGCTCT
GAAGAAGAAGGAGTTCAACTTAGTTTATCAGCCAGTGTTCGATAATCTAAAGCAGGAGGTTATTGGTGTTGAGGTGCTAT
TAAGATGGTTAAGGCCCGATGGCACAACACGTAATACTGGAGAGTTTATTGGGATCCTTGAGAAAGATCCTATTATGCCA
GAGGTCACTCGCTGGGTCGTTAAAATGGCATTGAATGAACTTAAAGAGCAATTAGATGGCAAGCTTATAGGCTGGTGCAG
TATCAATGTCAGCGCAATGGAAATTGAGCAGGGACAGATGCTTCCATTTTTAAGGGAATTGGTGGAAGAGGGATATCCAG
TAGAATACTTGAGCTTCGAGTTGACAGAGCGGATACCAATGAATAACTGGCAGCAACTTGAAGACTTTATTAAAGCATGC
CAAGCACTAGGATGTAAGGTCAAACTGGATGATGTGGGAACTGGCTATGGTGGTAGTCTGTGTTTACAGAATTTAGACTT
CGATTACCTGAAAATCGATCAACAGTTTGTTGGTCACTTAGGCACGCCAGAAAGTAAGCTATCTCTAATCGAGTCATATA
TTGCGATTGCTAAAGAATTAAATATTAGTGTCATCGCCGAAGGGGTAGAAACCTTAGAACAGGCTGAGATCCTGAAAACA
CTCGGTGTTAATCTGCAGCAGGGCTGGCTTTATTCAAAGGCATTGCCAGCTAGAGAGCTTGAAGCGTACTTACTTAAAGC
GACTTAA

Upstream 100 bases:

>100_bases
GGTTTCACTCTGATCCTTTCCGGAGGTTCTGGTGTTGCCTGTAATAGCACAGGGCTCCGTTTACCGTTTAGCTGTTTTCA
ACCAGTGAAGGCTTAGCTAT

Downstream 100 bases:

>100_bases
TCTCGCTATCGAGACAAAAAGCCTGAGTGTTATTACACTTAGGCTTTTTGTTGTCTGGCGCTCAGGCTTCTGATACTCAA
ATATCGACTTACTTTGACAA

Product: diguanylate phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MLSHVLNQRHSSWLVFALLMLCLIVSYILTQYQLYKKLEVRVNDFDNFYSERLESADRSLLAVEQVLKNIEVGRCSQGVV
TSLKDYILTSDALAIVWVNFIDEPILCSALGVSQLPDPKYYPVIASMGNGLKIYQKVDSDNPDERKPVYVGRHTEQYRWF
IGIDVLSYIQEILKNCEDCGALSIQFNSKHELFRKNKSAPYWGAMQFNSEVTGLTYRFSLNLYSKALLWLTHFVGVIAIS
SMFFVGWLLLRGVSRQYYWHRLFLKALKKKEFNLVYQPVFDNLKQEVIGVEVLLRWLRPDGTTRNTGEFIGILEKDPIMP
EVTRWVVKMALNELKEQLDGKLIGWCSINVSAMEIEQGQMLPFLRELVEEGYPVEYLSFELTERIPMNNWQQLEDFIKAC
QALGCKVKLDDVGTGYGGSLCLQNLDFDYLKIDQQFVGHLGTPESKLSLIESYIAIAKELNISVIAEGVETLEQAEILKT
LGVNLQQGWLYSKALPARELEAYLLKAT

Sequences:

>Translated_508_residues
MLSHVLNQRHSSWLVFALLMLCLIVSYILTQYQLYKKLEVRVNDFDNFYSERLESADRSLLAVEQVLKNIEVGRCSQGVV
TSLKDYILTSDALAIVWVNFIDEPILCSALGVSQLPDPKYYPVIASMGNGLKIYQKVDSDNPDERKPVYVGRHTEQYRWF
IGIDVLSYIQEILKNCEDCGALSIQFNSKHELFRKNKSAPYWGAMQFNSEVTGLTYRFSLNLYSKALLWLTHFVGVIAIS
SMFFVGWLLLRGVSRQYYWHRLFLKALKKKEFNLVYQPVFDNLKQEVIGVEVLLRWLRPDGTTRNTGEFIGILEKDPIMP
EVTRWVVKMALNELKEQLDGKLIGWCSINVSAMEIEQGQMLPFLRELVEEGYPVEYLSFELTERIPMNNWQQLEDFIKAC
QALGCKVKLDDVGTGYGGSLCLQNLDFDYLKIDQQFVGHLGTPESKLSLIESYIAIAKELNISVIAEGVETLEQAEILKT
LGVNLQQGWLYSKALPARELEAYLLKAT
>Mature_508_residues
MLSHVLNQRHSSWLVFALLMLCLIVSYILTQYQLYKKLEVRVNDFDNFYSERLESADRSLLAVEQVLKNIEVGRCSQGVV
TSLKDYILTSDALAIVWVNFIDEPILCSALGVSQLPDPKYYPVIASMGNGLKIYQKVDSDNPDERKPVYVGRHTEQYRWF
IGIDVLSYIQEILKNCEDCGALSIQFNSKHELFRKNKSAPYWGAMQFNSEVTGLTYRFSLNLYSKALLWLTHFVGVIAIS
SMFFVGWLLLRGVSRQYYWHRLFLKALKKKEFNLVYQPVFDNLKQEVIGVEVLLRWLRPDGTTRNTGEFIGILEKDPIMP
EVTRWVVKMALNELKEQLDGKLIGWCSINVSAMEIEQGQMLPFLRELVEEGYPVEYLSFELTERIPMNNWQQLEDFIKAC
QALGCKVKLDDVGTGYGGSLCLQNLDFDYLKIDQQFVGHLGTPESKLSLIESYIAIAKELNISVIAEGVETLEQAEILKT
LGVNLQQGWLYSKALPARELEAYLLKAT

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG4943

COG function: function code T; Predicted signal transduction protein containing sensor and EAL domains

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790496, Length=240, Percent_Identity=33.75, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87081980, Length=303, Percent_Identity=28.0528052805281, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI87081921, Length=266, Percent_Identity=29.6992481203008, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI87081743, Length=280, Percent_Identity=28.9285714285714, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1788502, Length=249, Percent_Identity=29.718875502008, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787541, Length=249, Percent_Identity=30.5220883534137, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI87081845, Length=271, Percent_Identity=30.9963099630996, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI226510982, Length=239, Percent_Identity=31.7991631799163, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786507, Length=267, Percent_Identity=30.7116104868914, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1788849, Length=234, Percent_Identity=27.3504273504274, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787055, Length=261, Percent_Identity=27.2030651340996, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082096, Length=232, Percent_Identity=25.8620689655172, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787410, Length=287, Percent_Identity=23.3449477351916, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 58263; Mature: 58263

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSHVLNQRHSSWLVFALLMLCLIVSYILTQYQLYKKLEVRVNDFDNFYSERLESADRSL
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LAVEQVLKNIEVGRCSQGVVTSLKDYILTSDALAIVWVNFIDEPILCSALGVSQLPDPKY
HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCC
YPVIASMGNGLKIYQKVDSDNPDERKPVYVGRHTEQYRWFIGIDVLSYIQEILKNCEDCG
CHHHHHCCCCEEEEEECCCCCCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHHHCHHHCC
ALSIQFNSKHELFRKNKSAPYWGAMQFNSEVTGLTYRFSLNLYSKALLWLTHFVGVIAIS
EEEEEECCHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
SMFFVGWLLLRGVSRQYYWHRLFLKALKKKEFNLVYQPVFDNLKQEVIGVEVLLRWLRPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHCCC
GTTRNTGEFIGILEKDPIMPEVTRWVVKMALNELKEQLDGKLIGWCSINVSAMEIEQGQM
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECEEEEEECCCCC
LPFLRELVEEGYPVEYLSFELTERIPMNNWQQLEDFIKACQALGCKVKLDDVGTGYGGSL
CHHHHHHHHCCCCEEEEHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHH
CLQNLDFDYLKIDQQFVGHLGTPESKLSLIESYIAIAKELNISVIAEGVETLEQAEILKT
HHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LGVNLQQGWLYSKALPARELEAYLLKAT
HCCCCCCCEEHHHCCCHHHHHHHHEECC
>Mature Secondary Structure
MLSHVLNQRHSSWLVFALLMLCLIVSYILTQYQLYKKLEVRVNDFDNFYSERLESADRSL
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LAVEQVLKNIEVGRCSQGVVTSLKDYILTSDALAIVWVNFIDEPILCSALGVSQLPDPKY
HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCC
YPVIASMGNGLKIYQKVDSDNPDERKPVYVGRHTEQYRWFIGIDVLSYIQEILKNCEDCG
CHHHHHCCCCEEEEEECCCCCCCCCCCEEEECCCCCEEEEEHHHHHHHHHHHHHCHHHCC
ALSIQFNSKHELFRKNKSAPYWGAMQFNSEVTGLTYRFSLNLYSKALLWLTHFVGVIAIS
EEEEEECCHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
SMFFVGWLLLRGVSRQYYWHRLFLKALKKKEFNLVYQPVFDNLKQEVIGVEVLLRWLRPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHCCC
GTTRNTGEFIGILEKDPIMPEVTRWVVKMALNELKEQLDGKLIGWCSINVSAMEIEQGQM
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECEEEEEECCCCC
LPFLRELVEEGYPVEYLSFELTERIPMNNWQQLEDFIKACQALGCKVKLDDVGTGYGGSL
CHHHHHHHHCCCCEEEEHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHH
CLQNLDFDYLKIDQQFVGHLGTPESKLSLIESYIAIAKELNISVIAEGVETLEQAEILKT
HHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LGVNLQQGWLYSKALPARELEAYLLKAT
HCCCCCCCEEHHHCCCHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]