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The map label for this gene is potF [H]

Identifier: 157961031

GI number: 157961031

Start: 1464553

End: 1465638

Strand: Direct

Name: potF [H]

Synonym: Spea_1203

Alternate gene names: 157961031

Gene position: 1464553-1465638 (Clockwise)

Preceding gene: 157961030

Following gene: 157961032

Centisome position: 28.3

GC content: 43.83

Gene sequence:

>1086_bases
ATGAATAACCAATCTAGCATGTTGTTAGCGACATTAGCGCTGTTCACCAGCTCGGTATCGGCGCAGAGTACTCTTAATAT
TTATAACTGGTCAGATTATATCAGCGAGGCGGCAATAGCCTCATTTGAAGCCAAAACAGGGATTAAAGTGAATTACGATG
TTTATGATTCTCAGGAGCTTGCTGAAACCAAATTGCTACTGGGTAAAACTGGCTACGACCTAGTGGTGTCATCAGGTTCA
TTCTTACAGCGGCAGATTAAGGTAGGAGTATTTCAGCCTTTAGATAAGAGTCGTTTAAAGAACTATTATCACCTCGATCC
TAAGGTCCTCAATATCGTTCAATCTTTTGATGAAGATAATCAGTATGCGGTTCCGTACATGTGGGGCACAACAGGTATTG
GTTATAACATAGATAAGGCGGCTCAAATATTAGGAGAGCAGCCCTTAAATACATGGGAACTCCTGTTTCAAGCCGAAACG
GTTTCAAAGTTTAGTGAGTGTGGTGTCGCCATGGTTGATGCCCCAAGCGAAGTCGTCAGTGCCATGTTGAAGTATCTAGG
CAAAAATCCTAACTCGCAGAATTTAGACGATCTAAAGTTGGTTGAGGAGCGGCTTCTTGCGATACGCCCCTATATTACCT
ATTTTCACAGCTCTTCTTACGTTACCGACCTTGCAAGTGGCAATATCTGCTTAGCTATGGGCTGGAGTGGTGATGTATTT
ATGGCTGCGAATAGAGCCCTTGAGGCTAACAATGGGGTAAATGTTGACTATGTGATCCCAAAAGAGGGCGCACAAGGTTG
GGCGGACACGTTTAATATCACTAATGACTCTAAAAATGTTGATGCAGCTTATGCCTTTTTGGACCATATCTTAGAGCCTA
AGGTCATCGCTGAGATCTCTAATTACGTCTGGTATGCCAATGCCAACGCTGACTCGCAGCAGTATGTCGATACCGCTATT
ACCTCTCATCCAGGTATTTATCCGGATGAAAACACCTTGAGTAACTTGTTTGCTGATGAGGGGCGTAGCTTGAAGTTTGA
ACGTGCGATGAATCGTATGTGGACTCGTGTCAAGACGGGCCAATAA

Upstream 100 bases:

>100_bases
GGGGAGGCAATGTCGCCTGTATTACTCAGCCACAGTACGCAGGTATTACCGCATAGAGCATACAGCAGAGATCAGAAACT
ATCAGCACAGGGAAGATAAG

Downstream 100 bases:

>100_bases
GCGCTCAAATTCACTGCGAGAGGGCTTCAGCATGAGTCTCTCTTAGCTCCCCCCTATTTTAGGCATTTAAAAGAGGCTTT
GTATGTCCAAACTTGTCAAA

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MNNQSSMLLATLALFTSSVSAQSTLNIYNWSDYISEAAIASFEAKTGIKVNYDVYDSQELAETKLLLGKTGYDLVVSSGS
FLQRQIKVGVFQPLDKSRLKNYYHLDPKVLNIVQSFDEDNQYAVPYMWGTTGIGYNIDKAAQILGEQPLNTWELLFQAET
VSKFSECGVAMVDAPSEVVSAMLKYLGKNPNSQNLDDLKLVEERLLAIRPYITYFHSSSYVTDLASGNICLAMGWSGDVF
MAANRALEANNGVNVDYVIPKEGAQGWADTFNITNDSKNVDAAYAFLDHILEPKVIAEISNYVWYANANADSQQYVDTAI
TSHPGIYPDENTLSNLFADEGRSLKFERAMNRMWTRVKTGQ

Sequences:

>Translated_361_residues
MNNQSSMLLATLALFTSSVSAQSTLNIYNWSDYISEAAIASFEAKTGIKVNYDVYDSQELAETKLLLGKTGYDLVVSSGS
FLQRQIKVGVFQPLDKSRLKNYYHLDPKVLNIVQSFDEDNQYAVPYMWGTTGIGYNIDKAAQILGEQPLNTWELLFQAET
VSKFSECGVAMVDAPSEVVSAMLKYLGKNPNSQNLDDLKLVEERLLAIRPYITYFHSSSYVTDLASGNICLAMGWSGDVF
MAANRALEANNGVNVDYVIPKEGAQGWADTFNITNDSKNVDAAYAFLDHILEPKVIAEISNYVWYANANADSQQYVDTAI
TSHPGIYPDENTLSNLFADEGRSLKFERAMNRMWTRVKTGQ
>Mature_361_residues
MNNQSSMLLATLALFTSSVSAQSTLNIYNWSDYISEAAIASFEAKTGIKVNYDVYDSQELAETKLLLGKTGYDLVVSSGS
FLQRQIKVGVFQPLDKSRLKNYYHLDPKVLNIVQSFDEDNQYAVPYMWGTTGIGYNIDKAAQILGEQPLNTWELLFQAET
VSKFSECGVAMVDAPSEVVSAMLKYLGKNPNSQNLDDLKLVEERLLAIRPYITYFHSSSYVTDLASGNICLAMGWSGDVF
MAANRALEANNGVNVDYVIPKEGAQGWADTFNITNDSKNVDAAYAFLDHILEPKVIAEISNYVWYANANADSQQYVDTAI
TSHPGIYPDENTLSNLFADEGRSLKFERAMNRMWTRVKTGQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=355, Percent_Identity=50.7042253521127, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1787367, Length=314, Percent_Identity=30.5732484076433, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1787711, Length=325, Percent_Identity=25.8461538461538, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40199; Mature: 40199

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNQSSMLLATLALFTSSVSAQSTLNIYNWSDYISEAAIASFEAKTGIKVNYDVYDSQEL
CCCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEECCCCHHH
AETKLLLGKTGYDLVVSSGSFLQRQIKVGVFQPLDKSRLKNYYHLDPKVLNIVQSFDEDN
HHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCHHHCCCEECCCHHHHHHHHHCCCCC
QYAVPYMWGTTGIGYNIDKAAQILGEQPLNTWELLFQAETVSKFSECGVAMVDAPSEVVS
CEEEEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCHHHHH
AMLKYLGKNPNSQNLDDLKLVEERLLAIRPYITYFHSSSYVTDLASGNICLAMGWSGDVF
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEHHCCCCEEEEECCCCCEE
MAANRALEANNGVNVDYVIPKEGAQGWADTFNITNDSKNVDAAYAFLDHILEPKVIAEIS
EEECCEEECCCCCEEEEEECCCCCCCCHHCEECCCCCCCHHHHHHHHHHHHCCHHHHHHH
NYVWYANANADSQQYVDTAITSHPGIYPDENTLSNLFADEGRSLKFERAMNRMWTRVKTG
CEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCC
Q
C
>Mature Secondary Structure
MNNQSSMLLATLALFTSSVSAQSTLNIYNWSDYISEAAIASFEAKTGIKVNYDVYDSQEL
CCCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEECCCCHHH
AETKLLLGKTGYDLVVSSGSFLQRQIKVGVFQPLDKSRLKNYYHLDPKVLNIVQSFDEDN
HHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCHHHCCCEECCCHHHHHHHHHCCCCC
QYAVPYMWGTTGIGYNIDKAAQILGEQPLNTWELLFQAETVSKFSECGVAMVDAPSEVVS
CEEEEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCHHHHH
AMLKYLGKNPNSQNLDDLKLVEERLLAIRPYITYFHSSSYVTDLASGNICLAMGWSGDVF
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEHHCCCCEEEEECCCCCEE
MAANRALEANNGVNVDYVIPKEGAQGWADTFNITNDSKNVDAAYAFLDHILEPKVIAEIS
EEECCEEECCCCCEEEEEECCCCCCCCHHCEECCCCCCCHHHHHHHHHHHHCCHHHHHHH
NYVWYANANADSQQYVDTAITSHPGIYPDENTLSNLFADEGRSLKFERAMNRMWTRVKTG
CEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]