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The map label for this gene is yafV [C]

Identifier: 157961032

GI number: 157961032

Start: 1465721

End: 1466593

Strand: Direct

Name: yafV [C]

Synonym: Spea_1204

Alternate gene names: 157961032

Gene position: 1465721-1466593 (Clockwise)

Preceding gene: 157961031

Following gene: 157961035

Centisome position: 28.33

GC content: 44.22

Gene sequence:

>873_bases
ATGTCCAAACTTGTCAAATTTGCAGCTATTCAACTCTCTATCAACTGGGATTTAGAACTTAATCTTTCAGAGGCAACTAA
AAGAGTTAAAGAGGCAGCAGCTAATGGTGCTCAAGTCATTTTGCTTCAAGAGTTATTCGCTGCACCTTACTTTTGCAAGC
AACAAAGTGCAAAGTATTTTGAATTAGCTGCCGAGATCGCAGAAAGCAGCTTAATACAAAAAATGAGTGAGTTGGCCAAG
TCCCTGCAAGTCGTCTTGCCTATCAGCTATTTCGAAAAGTCTGGCAATAACTTCTTTAATTCACTGGTAATGATAGATGC
AGATGGCACGATTTTAGATAACTATCGTAAGTCCCATATTCCCGATGGCCCAGGTTATAGTGAGAAGTACTACTTCAGCC
CGGGAGACACAGGCTTTAAGGTATGGGACACCCGCTGCGGTCGCTTTGGAGCGGGGATCTGTTGGGATCAATGGTTTCCT
GAGTTAGCACGCTGTTTGACTCTGGCAGGGGCTGAGGCAATATTCTACCCAACGGCTATTGGATCAGAGCCTCAAGCGCC
AAGCTTAGATTCTAAAGGACATTGGCAGCGAACAATGCAAGGTCATGCTGCCGCAAATTTAATACCCGTTATTGTGGCCA
ATCGTACTGGTGTTGAAACCGATGACGGAGTTGAAACTCGGTTTTATGGTTCCTCTTTTATCACCGATCACACGGGAGAG
TTATTAGCTGAAGCTAGCCGAAATAATGAGGAGGTTATTTATGCAGAAATCGATCTTCACGCGACTCACCTTGCCCGCTA
TAGTTGGGGAGTATTTAGAGATAGGCGGCCAGATCTATACTCACCACTATTAACATTGACAGGTAAAAGTTAG

Upstream 100 bases:

>100_bases
GTCAAGACGGGCCAATAAGCGCTCAAATTCACTGCGAGAGGGCTTCAGCATGAGTCTCTCTTAGCTCCCCCCTATTTTAG
GCATTTAAAAGAGGCTTTGT

Downstream 100 bases:

>100_bases
CAGTAACATTGATAGGCAATGGGCTTAACTAGAACGTTTATTAAGCCTTTAGTCAGTTGTGTACGCTAATTTAACTCAAT
CAGCTTCGGCACGGTTTGAT

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSKLVKFAAIQLSINWDLELNLSEATKRVKEAAANGAQVILLQELFAAPYFCKQQSAKYFELAAEIAESSLIQKMSELAK
SLQVVLPISYFEKSGNNFFNSLVMIDADGTILDNYRKSHIPDGPGYSEKYYFSPGDTGFKVWDTRCGRFGAGICWDQWFP
ELARCLTLAGAEAIFYPTAIGSEPQAPSLDSKGHWQRTMQGHAAANLIPVIVANRTGVETDDGVETRFYGSSFITDHTGE
LLAEASRNNEEVIYAEIDLHATHLARYSWGVFRDRRPDLYSPLLTLTGKS

Sequences:

>Translated_290_residues
MSKLVKFAAIQLSINWDLELNLSEATKRVKEAAANGAQVILLQELFAAPYFCKQQSAKYFELAAEIAESSLIQKMSELAK
SLQVVLPISYFEKSGNNFFNSLVMIDADGTILDNYRKSHIPDGPGYSEKYYFSPGDTGFKVWDTRCGRFGAGICWDQWFP
ELARCLTLAGAEAIFYPTAIGSEPQAPSLDSKGHWQRTMQGHAAANLIPVIVANRTGVETDDGVETRFYGSSFITDHTGE
LLAEASRNNEEVIYAEIDLHATHLARYSWGVFRDRRPDLYSPLLTLTGKS
>Mature_289_residues
SKLVKFAAIQLSINWDLELNLSEATKRVKEAAANGAQVILLQELFAAPYFCKQQSAKYFELAAEIAESSLIQKMSELAKS
LQVVLPISYFEKSGNNFFNSLVMIDADGTILDNYRKSHIPDGPGYSEKYYFSPGDTGFKVWDTRCGRFGAGICWDQWFPE
LARCLTLAGAEAIFYPTAIGSEPQAPSLDSKGHWQRTMQGHAAANLIPVIVANRTGVETDDGVETRFYGSSFITDHTGEL
LAEASRNNEEVIYAEIDLHATHLARYSWGVFRDRRPDLYSPLLTLTGKS

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=282, Percent_Identity=32.9787234042553, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI5031947, Length=294, Percent_Identity=27.891156462585, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI297632348, Length=294, Percent_Identity=27.891156462585, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI297632350, Length=294, Percent_Identity=27.891156462585, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI7706509, Length=302, Percent_Identity=26.4900662251656, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI297632346, Length=206, Percent_Identity=27.6699029126214, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17533173, Length=310, Percent_Identity=29.6774193548387, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17556280, Length=285, Percent_Identity=26.6666666666667, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323383, Length=294, Percent_Identity=29.5918367346939, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322335, Length=312, Percent_Identity=25.6410256410256, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21358471, Length=306, Percent_Identity=28.1045751633987, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21355835, Length=289, Percent_Identity=28.0276816608997, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI17933642, Length=280, Percent_Identity=25.3571428571429, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32270; Mature: 32138

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVKFAAIQLSINWDLELNLSEATKRVKEAAANGAQVILLQELFAAPYFCKQQSAKYF
CCCCEEEEEEEEEEEEEEEECHHHHHHHHHHHHCCCCEEEEEHHHHCCCCHHHHCCCHHH
ELAAEIAESSLIQKMSELAKSLQVVLPISYFEKSGNNFFNSLVMIDADGTILDNYRKSHI
HHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHCCCCCCEEEEEEEECCCCCHHCHHHHCC
PDGPGYSEKYYFSPGDTGFKVWDTRCGRFGAGICWDQWFPELARCLTLAGAEAIFYPTAI
CCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
GSEPQAPSLDSKGHWQRTMQGHAAANLIPVIVANRTGVETDDGVETRFYGSSFITDHTGE
CCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCHHH
LLAEASRNNEEVIYAEIDLHATHLARYSWGVFRDRRPDLYSPLLTLTGKS
HHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCCHHCCCEEECCCC
>Mature Secondary Structure 
SKLVKFAAIQLSINWDLELNLSEATKRVKEAAANGAQVILLQELFAAPYFCKQQSAKYF
CCCEEEEEEEEEEEEEEEECHHHHHHHHHHHHCCCCEEEEEHHHHCCCCHHHHCCCHHH
ELAAEIAESSLIQKMSELAKSLQVVLPISYFEKSGNNFFNSLVMIDADGTILDNYRKSHI
HHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHCCCCCCEEEEEEEECCCCCHHCHHHHCC
PDGPGYSEKYYFSPGDTGFKVWDTRCGRFGAGICWDQWFPELARCLTLAGAEAIFYPTAI
CCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
GSEPQAPSLDSKGHWQRTMQGHAAANLIPVIVANRTGVETDDGVETRFYGSSFITDHTGE
CCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCHHH
LLAEASRNNEEVIYAEIDLHATHLARYSWGVFRDRRPDLYSPLLTLTGKS
HHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCCHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]