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The map label for this gene is rpoS [H]

Identifier: 157961021

GI number: 157961021

Start: 1447036

End: 1448004

Strand: Direct

Name: rpoS [H]

Synonym: Spea_1193

Alternate gene names: 157961021

Gene position: 1447036-1448004 (Clockwise)

Preceding gene: 157961020

Following gene: 157961023

Centisome position: 27.96

GC content: 42.52

Gene sequence:

>969_bases
ATGGGCAGATTAAATAGTGCCGTTGTTGCAGAGCAGCCAAAAAATGACTCTGTAAAAGATTCATCGAATACTCTTACTAA
ACAGGATGTGAAAGAAGCTGAATTAGAGCAGCAAGTGCAAGATGATTTACAAAAAAACTTAGATGCCACACAGCTGTATC
TAGGTGAGATTGGATTTTCCCCTCTCCTCAGTGCCGAGGAAGAAGTGTATTTTTCACGTAAGTCCCTTAAAGGGTGTGAA
AAATCTCGTAACCGTATGATTGAAAGTAACCTCAGATTAGTCGTAAAAATTGCACGTCGATATAATAATCGTGGCTTAGC
GTTACTCGATCTTATCGAAGAGGGCAATTTAGGCCTTATCCGTGCAGTAGAGAAGTTCGATCCTGAGCGTGGCTTTAGAT
TTTCAACCTATGCAACATGGTGGATTAGACAGACGATTGAGCGAGCAATTATGAATCAGACTCGTACCATTCGTTTACCC
ATCCATGTGGTAAAAGAGCTGAATGTTTATCTGCGTACCGCAAGAGAGCTAGCGCATAAATTAGATCACGAGCCTACGGC
AGAAGAGATTGCAGATAAGCTAGATTTACCTAGTGCGGATGTAAGCCGTATGCTAAAGCTTAATGAAAGAATCACCTCTG
TTGACACCCCCTTAGGTGGCGAAAGTGATAAAGCATTACTAGATGTGCTTGCCGATGATGATAACGTAGGGCCTGACTAT
AAGGTGCAGGACGAAGACATGTCCAAGTCTGTAGTCAAATGGTTAGATGAGCTTAATACCAAGCAAAGAGAAGTGTTAGC
GCGCCGATTTGGTTTATTGGGCTACGAGCCATCTACGCTTGAAAACGTAGGTAAAGAAATTGGCTTAACCCGTGAAAGAG
TTCGTCAAATTCAAGTCGAGGCTCTCAAGCGTCTGAAAGATTTACTGGGGGCACAAGGCCTTTCTGTTGAAGCCATTTTT
AAAGCATAA

Upstream 100 bases:

>100_bases
GTTAAATTTTTGCCTAAACAATAGTTGTTGGGCCGCCATTTTGGCAAATCGGAGGATAGTGAAGTGCAGTAATTTAATGT
GAAAATTTGGGAGAAATATT

Downstream 100 bases:

>100_bases
AATTAAAGCATAAAATACTGTCTCGTTCTGAGATAAATAAAAAGCGCAGTTGAAATCAACTGCGCTTTTTTTGTGCCTAT
TGTCTAGTGCCCTAGAGGCT

Product: RpoD family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MGRLNSAVVAEQPKNDSVKDSSNTLTKQDVKEAELEQQVQDDLQKNLDATQLYLGEIGFSPLLSAEEEVYFSRKSLKGCE
KSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLP
IHVVKELNVYLRTARELAHKLDHEPTAEEIADKLDLPSADVSRMLKLNERITSVDTPLGGESDKALLDVLADDDNVGPDY
KVQDEDMSKSVVKWLDELNTKQREVLARRFGLLGYEPSTLENVGKEIGLTRERVRQIQVEALKRLKDLLGAQGLSVEAIF
KA

Sequences:

>Translated_322_residues
MGRLNSAVVAEQPKNDSVKDSSNTLTKQDVKEAELEQQVQDDLQKNLDATQLYLGEIGFSPLLSAEEEVYFSRKSLKGCE
KSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLP
IHVVKELNVYLRTARELAHKLDHEPTAEEIADKLDLPSADVSRMLKLNERITSVDTPLGGESDKALLDVLADDDNVGPDY
KVQDEDMSKSVVKWLDELNTKQREVLARRFGLLGYEPSTLENVGKEIGLTRERVRQIQVEALKRLKDLLGAQGLSVEAIF
KA
>Mature_321_residues
GRLNSAVVAEQPKNDSVKDSSNTLTKQDVKEAELEQQVQDDLQKNLDATQLYLGEIGFSPLLSAEEEVYFSRKSLKGCEK
SRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPI
HVVKELNVYLRTARELAHKLDHEPTAEEIADKLDLPSADVSRMLKLNERITSVDTPLGGESDKALLDVLADDDNVGPDYK
VQDEDMSKSVVKWLDELNTKQREVLARRFGLLGYEPSTLENVGKEIGLTRERVRQIQVEALKRLKDLLGAQGLSVEAIFK
A

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=289, Percent_Identity=78.8927335640138, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1789448, Length=226, Percent_Identity=42.0353982300885, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1789871, Length=294, Percent_Identity=28.2312925170068, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36605; Mature: 36474

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLNSAVVAEQPKNDSVKDSSNTLTKQDVKEAELEQQVQDDLQKNLDATQLYLGEIGFS
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
PLLSAEEEVYFSRKSLKGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLI
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCHHH
RAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRTARELAHK
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LDHEPTAEEIADKLDLPSADVSRMLKLNERITSVDTPLGGESDKALLDVLADDDNVGPDY
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCC
KVQDEDMSKSVVKWLDELNTKQREVLARRFGLLGYEPSTLENVGKEIGLTRERVRQIQVE
EECCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHH
ALKRLKDLLGAQGLSVEAIFKA
HHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure 
GRLNSAVVAEQPKNDSVKDSSNTLTKQDVKEAELEQQVQDDLQKNLDATQLYLGEIGFS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
PLLSAEEEVYFSRKSLKGCEKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLI
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCHHH
RAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRTARELAHK
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LDHEPTAEEIADKLDLPSADVSRMLKLNERITSVDTPLGGESDKALLDVLADDDNVGPDY
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCC
KVQDEDMSKSVVKWLDELNTKQREVLARRFGLLGYEPSTLENVGKEIGLTRERVRQIQVE
EECCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHH
ALKRLKDLLGAQGLSVEAIFKA
HHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]