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The map label for this gene is ispF [H]

Identifier: 157961016

GI number: 157961016

Start: 1442477

End: 1442956

Strand: Direct

Name: ispF [H]

Synonym: Spea_1188

Alternate gene names: 157961016

Gene position: 1442477-1442956 (Clockwise)

Preceding gene: 157961015

Following gene: 157961017

Centisome position: 27.88

GC content: 46.46

Gene sequence:

>480_bases
ATGAAAATTAGAATTGGTCATGGCTTTGATGTTCACAAGTTTGGTGGCGAGCCACCACTTATTCTCGGTGGCATCACGGT
GCCTTATGAGGTCGGTTTAATCGCACACTCTGATGGTGATGTGGTTTTGCATGCTATCTCGGATGCGATTCTTGGCGCGA
TGGCGCTTGGAGATATCGGTAAGCATTTTCCCGATACTGACCCCGACTTTAAGGGCGCCGATAGCCGAGTGCTATTGAGG
CACTGCTATAAATTAGCGATGGATATGGGCTATAGCTTATCAAACCTTGATGTGACCGTAATTGCTCAAGCGCCTAAAAT
GGCGCCGCATATCGAAGCCATTCGCGCTGTGCTAGCCGAAGATTTGGCATCGGAGATTTGCGATATTAATGTTAAAGCGA
CCACCACCGAAAAATTAGGTTTTACCGGCAGAAAAGAAGGCATTGCAGTAGAAGCTGTGGTGTTGATGATTAAATCATAA

Upstream 100 bases:

>100_bases
GACGATCTGCAGCTAGCCTCGCTATTTCTTAAAAGTGCAGCTCAATAGGCTAACTTAGAAACTAATTCGCTAAGAATTTA
ATAGATAAATAGGAACACCG

Downstream 100 bases:

>100_bases
TAAAGCTAAGCCATAACAAAGCTAAGTTATAAAAAGATTAAGTCATAAAAAGATTAAGTCATAAAAAGATTAAGTCATAG
TAAGAAGATAAACAGATTAT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MKIRIGHGFDVHKFGGEPPLILGGITVPYEVGLIAHSDGDVVLHAISDAILGAMALGDIGKHFPDTDPDFKGADSRVLLR
HCYKLAMDMGYSLSNLDVTVIAQAPKMAPHIEAIRAVLAEDLASEICDINVKATTTEKLGFTGRKEGIAVEAVVLMIKS

Sequences:

>Translated_159_residues
MKIRIGHGFDVHKFGGEPPLILGGITVPYEVGLIAHSDGDVVLHAISDAILGAMALGDIGKHFPDTDPDFKGADSRVLLR
HCYKLAMDMGYSLSNLDVTVIAQAPKMAPHIEAIRAVLAEDLASEICDINVKATTTEKLGFTGRKEGIAVEAVVLMIKS
>Mature_159_residues
MKIRIGHGFDVHKFGGEPPLILGGITVPYEVGLIAHSDGDVVLHAISDAILGAMALGDIGKHFPDTDPDFKGADSRVLLR
HCYKLAMDMGYSLSNLDVTVIAQAPKMAPHIEAIRAVLAEDLASEICDINVKATTTEKLGFTGRKEGIAVEAVVLMIKS

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=68.7898089171974, Blast_Score=205, Evalue=9e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 16947; Mature: 16947

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRIGHGFDVHKFGGEPPLILGGITVPYEVGLIAHSDGDVVLHAISDAILGAMALGDIG
CEEECCCCCCHHCCCCCCCEEEECEECCEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHH
KHFPDTDPDFKGADSRVLLRHCYKLAMDMGYSLSNLDVTVIAQAPKMAPHIEAIRAVLAE
HCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
DLASEICDINVKATTTEKLGFTGRKEGIAVEAVVLMIKS
HHHHHHHCCCCEECCHHHCCCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MKIRIGHGFDVHKFGGEPPLILGGITVPYEVGLIAHSDGDVVLHAISDAILGAMALGDIG
CEEECCCCCCHHCCCCCCCEEEECEECCEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHH
KHFPDTDPDFKGADSRVLLRHCYKLAMDMGYSLSNLDVTVIAQAPKMAPHIEAIRAVLAE
HCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
DLASEICDINVKATTTEKLGFTGRKEGIAVEAVVLMIKS
HHHHHHHCCCCEECCHHHCCCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA