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The map label for this gene is truD [H]

Identifier: 157961017

GI number: 157961017

Start: 1443055

End: 1444119

Strand: Direct

Name: truD [H]

Synonym: Spea_1189

Alternate gene names: 157961017

Gene position: 1443055-1444119 (Clockwise)

Preceding gene: 157961016

Following gene: 157961018

Centisome position: 27.89

GC content: 46.57

Gene sequence:

>1065_bases
ATGAGCGAACTACATTATTTATACGGCAAGCCGACATCGACAGCCGACCTGCGCACCCACAATAGTGACTTTCAGGTACA
AGAAATTCTACCATTTGCCCCTACGGGTGAAGGTGAGCACCATTTACTGCATATTCGCAAAGAGGGACTCAACACAGTTG
AAGTGGCTAAAATGCTATCTGGCTTTGCTCATGTGCACCCGAAAGAGGTGACCTACGCAGGGCAAAAAGATAAGCACGCG
ATCACCGAGCAATGGTTTGGCGTACGTATTCCCGGAAAGGAAACACCAAACTGGGAGCAACTAAATAGCGAACAGATCAC
TGTACTTAGCTCCTCTAGACACAGTAAGAAGCTACGTATTGGTGCGTTGGCTGGTAATCGTTTTACCTTAACCTTGCGCA
ATGTCTCTGATATGAGTGATGTGGTTGCGCGCATTGAGAAGATTGGTGAGCAGGGTGTTCCAAACTACTTTGGTGAGCAG
CGCTTTGGCCATGACGGCAAAAACCTTATATTTGGTCGTCAGATGTTTACAGGCCGTAAAGTGAAAGACAGAAACAAACG
TAGTATGTATCTTTCTGCTGTGCGCTCAAATCTATTCAATATGGCTGTATCCACGCGTTTAGCCCAACATGGTACAGGCA
CTCTTACTGGCGACTGTGTCATGCTTGCTGGGAGCAAGAGTTATTTTGTGGCTGAGCAGTGGGATGAAACTTTAGTCGAG
CGTTTAGCTAAAAAAGACATACAACTGTCGGCGCCATTATGGGGACGTGGTTTACCGCTTGCACAAGCTGAGGCTGCCGA
GTGTGAGGCGCAAGCGCTTGAATCACTTGCAACTGATAAAGATGGTTTAGAGCATGCGGGGCTTAGCCAAGAAAGACGCG
CATTGTTACTTGAGCCACAACATCTGCAATATGAAGTAGACGAAGATACCATTATTCTAAAATTTGCATTACCATCAGGT
TCTTATGCTACATCGGTACTACGAGAGTTATTTGTTTACCAGGATGTTCAGGAAGTCGCTCGAAAGCAGATGCTAGCCGC
ACAAAATAGCGGCGAGGCACAATGA

Upstream 100 bases:

>100_bases
AATAAAGCTAAGCCATAACAAAGCTAAGTTATAAAAAGATTAAGTCATAAAAAGATTAAGTCATAAAAAGATTAAGTCAT
AGTAAGAAGATAAACAGATT

Downstream 100 bases:

>100_bases
TGAAGATCCTTATCAGTAATGATGATGGAGTGAATGCGATAGGCATTGTTGCTCTGACTCGGTCTTTGAGCCAAATAGCA
GAAACACTCACTGTCGGGCC

Product: tRNA pseudouridine synthase D TruD

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSELHYLYGKPTSTADLRTHNSDFQVQEILPFAPTGEGEHHLLHIRKEGLNTVEVAKMLSGFAHVHPKEVTYAGQKDKHA
ITEQWFGVRIPGKETPNWEQLNSEQITVLSSSRHSKKLRIGALAGNRFTLTLRNVSDMSDVVARIEKIGEQGVPNYFGEQ
RFGHDGKNLIFGRQMFTGRKVKDRNKRSMYLSAVRSNLFNMAVSTRLAQHGTGTLTGDCVMLAGSKSYFVAEQWDETLVE
RLAKKDIQLSAPLWGRGLPLAQAEAAECEAQALESLATDKDGLEHAGLSQERRALLLEPQHLQYEVDEDTIILKFALPSG
SYATSVLRELFVYQDVQEVARKQMLAAQNSGEAQ

Sequences:

>Translated_354_residues
MSELHYLYGKPTSTADLRTHNSDFQVQEILPFAPTGEGEHHLLHIRKEGLNTVEVAKMLSGFAHVHPKEVTYAGQKDKHA
ITEQWFGVRIPGKETPNWEQLNSEQITVLSSSRHSKKLRIGALAGNRFTLTLRNVSDMSDVVARIEKIGEQGVPNYFGEQ
RFGHDGKNLIFGRQMFTGRKVKDRNKRSMYLSAVRSNLFNMAVSTRLAQHGTGTLTGDCVMLAGSKSYFVAEQWDETLVE
RLAKKDIQLSAPLWGRGLPLAQAEAAECEAQALESLATDKDGLEHAGLSQERRALLLEPQHLQYEVDEDTIILKFALPSG
SYATSVLRELFVYQDVQEVARKQMLAAQNSGEAQ
>Mature_353_residues
SELHYLYGKPTSTADLRTHNSDFQVQEILPFAPTGEGEHHLLHIRKEGLNTVEVAKMLSGFAHVHPKEVTYAGQKDKHAI
TEQWFGVRIPGKETPNWEQLNSEQITVLSSSRHSKKLRIGALAGNRFTLTLRNVSDMSDVVARIEKIGEQGVPNYFGEQR
FGHDGKNLIFGRQMFTGRKVKDRNKRSMYLSAVRSNLFNMAVSTRLAQHGTGTLTGDCVMLAGSKSYFVAEQWDETLVER
LAKKDIQLSAPLWGRGLPLAQAEAAECEAQALESLATDKDGLEHAGLSQERRALLLEPQHLQYEVDEDTIILKFALPSGS
YATSVLRELFVYQDVQEVARKQMLAAQNSGEAQ

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Escherichia coli, GI1789102, Length=338, Percent_Identity=46.1538461538462, Blast_Score=262, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 39562; Mature: 39431

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS50984 TRUD ; PS01268 UPF0024

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELHYLYGKPTSTADLRTHNSDFQVQEILPFAPTGEGEHHLLHIRKEGLNTVEVAKMLS
CCCCEEEECCCCCCCCCEECCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
GFAHVHPKEVTYAGQKDKHAITEQWFGVRIPGKETPNWEQLNSEQITVLSSSRHSKKLRI
HHHHCCCCCEEECCCCCCHHHHHHHCEEECCCCCCCCHHHCCCCEEEEEECCCCCCEEEE
GALAGNRFTLTLRNVSDMSDVVARIEKIGEQGVPNYFGEQRFGHDGKNLIFGRQMFTGRK
EEEECCEEEEEECCHHHHHHHHHHHHHHHCCCCCCHHCCHHCCCCCCCEEEEHHHHCCCC
VKDRNKRSMYLSAVRSNLFNMAVSTRLAQHGTGTLTGDCVMLAGSKSYFVAEQWDETLVE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEHHHHHHHHHH
RLAKKDIQLSAPLWGRGLPLAQAEAAECEAQALESLATDKDGLEHAGLSQERRALLLEPQ
HHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHEEEECCC
HLQYEVDEDTIILKFALPSGSYATSVLRELFVYQDVQEVARKQMLAAQNSGEAQ
CCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SELHYLYGKPTSTADLRTHNSDFQVQEILPFAPTGEGEHHLLHIRKEGLNTVEVAKMLS
CCCEEEECCCCCCCCCEECCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
GFAHVHPKEVTYAGQKDKHAITEQWFGVRIPGKETPNWEQLNSEQITVLSSSRHSKKLRI
HHHHCCCCCEEECCCCCCHHHHHHHCEEECCCCCCCCHHHCCCCEEEEEECCCCCCEEEE
GALAGNRFTLTLRNVSDMSDVVARIEKIGEQGVPNYFGEQRFGHDGKNLIFGRQMFTGRK
EEEECCEEEEEECCHHHHHHHHHHHHHHHCCCCCCHHCCHHCCCCCCCEEEEHHHHCCCC
VKDRNKRSMYLSAVRSNLFNMAVSTRLAQHGTGTLTGDCVMLAGSKSYFVAEQWDETLVE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEHHHHHHHHHH
RLAKKDIQLSAPLWGRGLPLAQAEAAECEAQALESLATDKDGLEHAGLSQERRALLLEPQ
HHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHEEEECCC
HLQYEVDEDTIILKFALPSGSYATSVLRELFVYQDVQEVARKQMLAAQNSGEAQ
CCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]