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The map label for this gene is ispH [H]

Identifier: 157960914

GI number: 157960914

Start: 1314577

End: 1315506

Strand: Direct

Name: ispH [H]

Synonym: Spea_1086

Alternate gene names: 157960914

Gene position: 1314577-1315506 (Clockwise)

Preceding gene: 157960913

Following gene: 157960915

Centisome position: 25.4

GC content: 47.96

Gene sequence:

>930_bases
ATGAATATTCTTCTGGCAAATCCTCGTGGTTTTTGTGCAGGTGTTGATCGCGCCATTAGTATTGTTGAGCGCGCATTAGA
GTTATTTTCTCCACCTATTTATGTCAGGCACGAAGTGGTTCATAACCGCTACGTGGTGCAGAACTTGAAAGAACGTGGTG
CCGTGTTTGTCGAAGAGCTAGATCAAGTACCAGACGATAGCATCGTTATCTTTAGTGCTCATGGTGTATCTCAAGCCGTT
CGAGCCGAAGCTAAGAGACGCGGCTTAAAGGTGTTCGATGCGACTTGTCCTCTTGTCACTAAGGTTCATCTACAGGTCGC
TCGTGCAAGCCGTAAAGGTATTGAGTGCATCTTAATTGGTCATGCAGGACACCCTGAAGTGGAAGGCACCATGGGTCAGT
ACGACAACTCTGCTGGTGGCGTACATTTAGTTGAGTCACCTGAAGATGTAGCAAAGCTTGTGGTCAAAGATCCTGACAAC
CTTTGCTTTGTGACTCAAACAACCTTATCAATGGACGATACCGCGGATGTGATCTCGTCTTTACAGAAAAAGTACCCTTC
AATCGAAGGGCCGCGTAAGGATGATATCTGCTACGCGACGCAGAACCGCCAAGACGCAGTTCGCAATGTTGCTGAAGAAG
TGGAACTATTTATCGTCGTTGGCTCCAAGAACAGTTCTAACTCAAATCGTCTGCGTGAGTTAGCACAGAAGAAAGGCACA
CAAGCTTTCTTAGTGGATAATGCCGATGACGTGGATGCCACATGGTTTAATGGCGTAGAACGCGTTGCCGTAACCGCTGG
GGCATCTGCGCCAGAGGTCTTGGTTAAGCAGGTTGTCGATGCGATTGCTAAAATGGCACCGAGCGTAGTAACAGAGGTCG
AAGGTCAACTCGAAGATACTGTTTTCGCGGTACCTGCTGAGCTTCGTTGA

Upstream 100 bases:

>100_bases
TGAGAGACGTGGTCGCTGACTCAGTGACCATAGATCTGAACCATCCACTCGCAGGTCAAGTGTTAACCTTTGAGCTTGAA
GTGGTACAGGTACTGTAATT

Downstream 100 bases:

>100_bases
GGCTTCGTGAAGTTGAGATACAAATTGAATTAAATAAAAAGAGGCTTAGGCCTCTTTTTTTTCGCTTAAAATTCAGCCAT
ATCTTGTATGTTAATCAGGA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MNILLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKERGAVFVEELDQVPDDSIVIFSAHGVSQAV
RAEAKRRGLKVFDATCPLVTKVHLQVARASRKGIECILIGHAGHPEVEGTMGQYDNSAGGVHLVESPEDVAKLVVKDPDN
LCFVTQTTLSMDDTADVISSLQKKYPSIEGPRKDDICYATQNRQDAVRNVAEEVELFIVVGSKNSSNSNRLRELAQKKGT
QAFLVDNADDVDATWFNGVERVAVTAGASAPEVLVKQVVDAIAKMAPSVVTEVEGQLEDTVFAVPAELR

Sequences:

>Translated_309_residues
MNILLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKERGAVFVEELDQVPDDSIVIFSAHGVSQAV
RAEAKRRGLKVFDATCPLVTKVHLQVARASRKGIECILIGHAGHPEVEGTMGQYDNSAGGVHLVESPEDVAKLVVKDPDN
LCFVTQTTLSMDDTADVISSLQKKYPSIEGPRKDDICYATQNRQDAVRNVAEEVELFIVVGSKNSSNSNRLRELAQKKGT
QAFLVDNADDVDATWFNGVERVAVTAGASAPEVLVKQVVDAIAKMAPSVVTEVEGQLEDTVFAVPAELR
>Mature_309_residues
MNILLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKERGAVFVEELDQVPDDSIVIFSAHGVSQAV
RAEAKRRGLKVFDATCPLVTKVHLQVARASRKGIECILIGHAGHPEVEGTMGQYDNSAGGVHLVESPEDVAKLVVKDPDN
LCFVTQTTLSMDDTADVISSLQKKYPSIEGPRKDDICYATQNRQDAVRNVAEEVELFIVVGSKNSSNSNRLRELAQKKGT
QAFLVDNADDVDATWFNGVERVAVTAGASAPEVLVKQVVDAIAKMAPSVVTEVEGQLEDTVFAVPAELR

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=66.9902912621359, Blast_Score=433, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 33644; Mature: 33644

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKERGAVFVEEL
CCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHH
DQVPDDSIVIFSAHGVSQAVRAEAKRRGLKVFDATCPLVTKVHLQVARASRKGIECILIG
HCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEE
HAGHPEVEGTMGQYDNSAGGVHLVESPEDVAKLVVKDPDNLCFVTQTTLSMDDTADVISS
CCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHH
LQKKYPSIEGPRKDDICYATQNRQDAVRNVAEEVELFIVVGSKNSSNSNRLRELAQKKGT
HHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCEEEEEEECCCCCCCHHHHHHHHHHCCC
QAFLVDNADDVDATWFNGVERVAVTAGASAPEVLVKQVVDAIAKMAPSVVTEVEGQLEDT
EEEEEECCCCCCHHHHCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHE
VFAVPAELR
EEECCCCCC
>Mature Secondary Structure
MNILLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKERGAVFVEEL
CCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHH
DQVPDDSIVIFSAHGVSQAVRAEAKRRGLKVFDATCPLVTKVHLQVARASRKGIECILIG
HCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEE
HAGHPEVEGTMGQYDNSAGGVHLVESPEDVAKLVVKDPDNLCFVTQTTLSMDDTADVISS
CCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHH
LQKKYPSIEGPRKDDICYATQNRQDAVRNVAEEVELFIVVGSKNSSNSNRLRELAQKKGT
HHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCEEEEEEECCCCCCCHHHHHHHHHHCCC
QAFLVDNADDVDATWFNGVERVAVTAGASAPEVLVKQVVDAIAKMAPSVVTEVEGQLEDT
EEEEEECCCCCCHHHHCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHE
VFAVPAELR
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA