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The map label for this gene is thyA [H]

Identifier: 157960852

GI number: 157960852

Start: 1247941

End: 1248792

Strand: Direct

Name: thyA [H]

Synonym: Spea_1024

Alternate gene names: 157960852

Gene position: 1247941-1248792 (Clockwise)

Preceding gene: 157960851

Following gene: 157960853

Centisome position: 24.12

GC content: 43.54

Gene sequence:

>852_bases
ATGAAACAGTATTTAGATCTTTGTCAGCGACTAATTGATGAAGGAACTTGGGTTGAGAATAGCCGAACAGGTAAACGTTG
CCTCACCGTGATTAATGCTGATTTGGTTTATCATGTGGATAAAAATGAGTTTCCACTCATCACCACCCGTAAGAGTTTTT
ACAAGGCAGCAATTGCAGAGTTACTTGGCTACATTCGTGGTTACGACAATGCAGCTGATTTTCGGGCGATAGGTTGCAAC
ACCTGGAACGCCAATGCTAACGACAATCAAGCATGGCTCGATAACCCACATCGTAAAGGCATCGATGATATGGGGCGTGT
TTATGGTGTGCAAGGCCGCGCTTGGAGTAAGCCCGATGGTGGATGTATCGATCAGCTACGCAAAATTGTTGATGACTTAT
CAAAGGGGATTGACGATAGAGGCGAGATTTTAAGCTTCTATAACCCTGGTGAATTCCACATGGGCTGTTTAAGACCTTGT
ATGCATACCCATAACTTCTCTTTAGTAGGTGATACGCTACACCTAACCAGTTTTCAGCGTTCGTGCGATGTACCCCTAGG
GCTGAACTTCAATCAAGTTCAAGTCTTTACGCTACTGGCCTTAATGGCGCAAATTACTGGGCATAAGCCTGGAACGGCAT
TTCATAAAATCGTTAATGCCCATATCTATGAAGATCAGTTAGAGTTAATGCAGGAGGTTCAGCTTAAGCGTGAGCCTTTT
ACTTCACCTAAACTTGTCATTAACCCTAATATTAAGTCACTCGAAGATCTTGAGACTTGGGTGTCTATAGATGATTTTGA
GGTGACAGGTTACGAGCACCACGAAGCGATTAGCTATCCATTCTCAGTGTAG

Upstream 100 bases:

>100_bases
AACTTGTTTTAGCATAATGTTCTACTGAGCTATTATTGGTAGCATTTCTTCGTTTGACGCTGGGAGTGAGCTCAGCAACT
CTTTTTATAGGTAGATAATC

Downstream 100 bases:

>100_bases
CTTTCTTGGCTCTAAAATAAGCTTTAAAAAACTCGCCTAATGGCGAGTTTTTTATCAATTTAGTTAAATCCTTTCAAGTC
ACCTCTCATACATTCCTGCT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKQYLDLCQRLIDEGTWVENSRTGKRCLTVINADLVYHVDKNEFPLITTRKSFYKAAIAELLGYIRGYDNAADFRAIGCN
TWNANANDNQAWLDNPHRKGIDDMGRVYGVQGRAWSKPDGGCIDQLRKIVDDLSKGIDDRGEILSFYNPGEFHMGCLRPC
MHTHNFSLVGDTLHLTSFQRSCDVPLGLNFNQVQVFTLLALMAQITGHKPGTAFHKIVNAHIYEDQLELMQEVQLKREPF
TSPKLVINPNIKSLEDLETWVSIDDFEVTGYEHHEAISYPFSV

Sequences:

>Translated_283_residues
MKQYLDLCQRLIDEGTWVENSRTGKRCLTVINADLVYHVDKNEFPLITTRKSFYKAAIAELLGYIRGYDNAADFRAIGCN
TWNANANDNQAWLDNPHRKGIDDMGRVYGVQGRAWSKPDGGCIDQLRKIVDDLSKGIDDRGEILSFYNPGEFHMGCLRPC
MHTHNFSLVGDTLHLTSFQRSCDVPLGLNFNQVQVFTLLALMAQITGHKPGTAFHKIVNAHIYEDQLELMQEVQLKREPF
TSPKLVINPNIKSLEDLETWVSIDDFEVTGYEHHEAISYPFSV
>Mature_283_residues
MKQYLDLCQRLIDEGTWVENSRTGKRCLTVINADLVYHVDKNEFPLITTRKSFYKAAIAELLGYIRGYDNAADFRAIGCN
TWNANANDNQAWLDNPHRKGIDDMGRVYGVQGRAWSKPDGGCIDQLRKIVDDLSKGIDDRGEILSFYNPGEFHMGCLRPC
MHTHNFSLVGDTLHLTSFQRSCDVPLGLNFNQVQVFTLLALMAQITGHKPGTAFHKIVNAHIYEDQLELMQEVQLKREPF
TSPKLVINPNIKSLEDLETWVSIDDFEVTGYEHHEAISYPFSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=291, Percent_Identity=32.9896907216495, Blast_Score=151, Evalue=7e-37,
Organism=Escherichia coli, GI1789191, Length=283, Percent_Identity=33.9222614840989, Blast_Score=154, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI71993377, Length=278, Percent_Identity=28.0575539568345, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324648, Length=301, Percent_Identity=27.2425249169435, Blast_Score=102, Evalue=7e-23,
Organism=Drosophila melanogaster, GI17137556, Length=292, Percent_Identity=31.1643835616438, Blast_Score=137, Evalue=8e-33,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 32212; Mature: 32212

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLCQRLIDEGTWVENSRTGKRCLTVINADLVYHVDKNEFPLITTRKSFYKAAIAE
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHEEECEEEEEECCCCCCEEECHHHHHHHHHHH
LLGYIRGYDNAADFRAIGCNTWNANANDNQAWLDNPHRKGIDDMGRVYGVQGRAWSKPDG
HHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHEECCCCCCCCCCC
GCIDQLRKIVDDLSKGIDDRGEILSFYNPGEFHMGCLRPCMHTHNFSLVGDTLHLTSFQR
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEECCCHHHHHHHH
SCDVPLGLNFNQVQVFTLLALMAQITGHKPGTAFHKIVNAHIYEDQLELMQEVQLKREPF
HCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TSPKLVINPNIKSLEDLETWVSIDDFEVTGYEHHEAISYPFSV
CCCEEEECCCCHHHHHHHHHHCCCCEEECCCCCCHHCCCCCCC
>Mature Secondary Structure
MKQYLDLCQRLIDEGTWVENSRTGKRCLTVINADLVYHVDKNEFPLITTRKSFYKAAIAE
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHEEECEEEEEECCCCCCEEECHHHHHHHHHHH
LLGYIRGYDNAADFRAIGCNTWNANANDNQAWLDNPHRKGIDDMGRVYGVQGRAWSKPDG
HHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHEECCCCCCCCCCC
GCIDQLRKIVDDLSKGIDDRGEILSFYNPGEFHMGCLRPCMHTHNFSLVGDTLHLTSFQR
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEECCCHHHHHHHH
SCDVPLGLNFNQVQVFTLLALMAQITGHKPGTAFHKIVNAHIYEDQLELMQEVQLKREPF
HCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TSPKLVINPNIKSLEDLETWVSIDDFEVTGYEHHEAISYPFSV
CCCEEEECCCCHHHHHHHHHHCCCCEEECCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA