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The map label for this gene is nhaA

Identifier: 157960853

GI number: 157960853

Start: 1249284

End: 1250459

Strand: Direct

Name: nhaA

Synonym: Spea_1025

Alternate gene names: 157960853

Gene position: 1249284-1250459 (Clockwise)

Preceding gene: 157960852

Following gene: 157960854

Centisome position: 24.14

GC content: 44.3

Gene sequence:

>1176_bases
ATGGAACGGGCAATTAGAAATTTTCTAAGCCAGGAATCGGCAGGCGGCATTCTCTTGATGGTTTCCGTCGTGCTAGCGAT
GATTTTAGCAAACTCACCTTTATCGGGTATGTACCATGGCTTTCTGGAAACAGAAATGCAAGTTCGTGTTGGTAACCTAG
ATATCGATAAGACACTTATTCATTGGATCAACGATGGTTTGATGGCTATCTTCTTCATGCTTATCGGCTTAGAAGTGAAA
AGAGAGCTACTCGAAGGAGCGTTATCTAGTCGAGCACAGGCATCTTTACCTACGTTTGCAGCGATTGGCGGTATGGTTTT
CCCAGCGGCTGTTTATCTTATTTTCAATTATTCTGATCCGATAACTCAAGTGGGTTGGGCTATTCCGGCTGCGACAGATA
TTGCATTTGCATTAGGTATTATGGCGCTATTAGGTAGCCGTGTGCCTGTGGCGTTAAAGGTGTTCTTGTTAGCACTTGCG
ATTATCGACGATCTGGGTGTAGTGGTAATCATTGCGCTTTTCTACAGTACAGACTTATCGACCATCAGCTTAATCATTGC
TGCTGCTGCGATTCTTGGCTTAATAGGCCTAAACCGTAAAGGAGTAACCTCATTAGCACCTTATGGCGTAGTGGGTCTTA
TTCTGTGGATTGCAGTATTAAAGTCTGGTGTACATGCCACATTAGCTGGCGTAATTATCGCCTTCTGTATTCCGCTTAGA
GCGAAAGACGGCTCGTCGCCATCAGAAAGCTTAGAGCACAGTTTGCACCCATGGAGTACCTTCATCATCTTACCAATATT
TGCCTTCGCGAATGCTGGCGTAGACTTGAGTGGCATGAGCCTAAGCTCACTCTTATCTCCTGTACCATTAGGTATTGCTT
TAGGACTACTGGTTGGTAAGCCGCTTGGTATTATGTTGTTTAGCTATATTGCAGTGAAGCTAAAGCTAGCGGTATTACCA
GAAGGTATGGGCTGGAGACACATTACACCTGTTGCTGTGATGTGTGGTATTGGTTTCACTATGTCTATGTTTATCTCATC
GCTTGCCTTTGTTGGTGAAGGGCAGATGTACGGAGATTATGCAAGATTAGGGATTTTATTGGGGTCAATAGCATCGGCAA
CCATAGGATATTTCTGGCTGTCTAAGGTGCTACCTGAAAAAGGAGCAAAAGCATGA

Upstream 100 bases:

>100_bases
ATACTTCGGATATTCCGACGTTTATGGCTGATGCAAACGATTATTCAGGTTAATCGTATTCAGCTAAACTGGAAGTATTG
AGATTTTATGGAGCGTAAAA

Downstream 100 bases:

>100_bases
GAATCACATTATTAGCATTAGTTGTAGGACTGGGCTTTAGCTCAGTGGCATCGGCCAAGCAGATTGAGGTTTGTAATCAG
GATATCAATAGCGTGGTATG

Product: pH-dependent sodium/proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MERAIRNFLSQESAGGILLMVSVVLAMILANSPLSGMYHGFLETEMQVRVGNLDIDKTLIHWINDGLMAIFFMLIGLEVK
RELLEGALSSRAQASLPTFAAIGGMVFPAAVYLIFNYSDPITQVGWAIPAATDIAFALGIMALLGSRVPVALKVFLLALA
IIDDLGVVVIIALFYSTDLSTISLIIAAAAILGLIGLNRKGVTSLAPYGVVGLILWIAVLKSGVHATLAGVIIAFCIPLR
AKDGSSPSESLEHSLHPWSTFIILPIFAFANAGVDLSGMSLSSLLSPVPLGIALGLLVGKPLGIMLFSYIAVKLKLAVLP
EGMGWRHITPVAVMCGIGFTMSMFISSLAFVGEGQMYGDYARLGILLGSIASATIGYFWLSKVLPEKGAKA

Sequences:

>Translated_391_residues
MERAIRNFLSQESAGGILLMVSVVLAMILANSPLSGMYHGFLETEMQVRVGNLDIDKTLIHWINDGLMAIFFMLIGLEVK
RELLEGALSSRAQASLPTFAAIGGMVFPAAVYLIFNYSDPITQVGWAIPAATDIAFALGIMALLGSRVPVALKVFLLALA
IIDDLGVVVIIALFYSTDLSTISLIIAAAAILGLIGLNRKGVTSLAPYGVVGLILWIAVLKSGVHATLAGVIIAFCIPLR
AKDGSSPSESLEHSLHPWSTFIILPIFAFANAGVDLSGMSLSSLLSPVPLGIALGLLVGKPLGIMLFSYIAVKLKLAVLP
EGMGWRHITPVAVMCGIGFTMSMFISSLAFVGEGQMYGDYARLGILLGSIASATIGYFWLSKVLPEKGAKA
>Mature_391_residues
MERAIRNFLSQESAGGILLMVSVVLAMILANSPLSGMYHGFLETEMQVRVGNLDIDKTLIHWINDGLMAIFFMLIGLEVK
RELLEGALSSRAQASLPTFAAIGGMVFPAAVYLIFNYSDPITQVGWAIPAATDIAFALGIMALLGSRVPVALKVFLLALA
IIDDLGVVVIIALFYSTDLSTISLIIAAAAILGLIGLNRKGVTSLAPYGVVGLILWIAVLKSGVHATLAGVIIAFCIPLR
AKDGSSPSESLEHSLHPWSTFIILPIFAFANAGVDLSGMSLSSLLSPVPLGIALGLLVGKPLGIMLFSYIAVKLKLAVLP
EGMGWRHITPVAVMCGIGFTMSMFISSLAFVGEGQMYGDYARLGILLGSIASATIGYFWLSKVLPEKGAKA

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family

Homologues:

Organism=Escherichia coli, GI1786201, Length=379, Percent_Identity=60.9498680738786, Blast_Score=427, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAA_SHEPA (A8H1B5)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001500887.1
- ProteinModelPortal:   A8H1B5
- SMR:   A8H1B5
- STRING:   A8H1B5
- GeneID:   5661424
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_1025
- NMPDR:   fig|398579.3.peg.893
- eggNOG:   COG3004
- HOGENOM:   HBG665619
- OMA:   LIHWIND
- ProtClustDB:   PRK09561
- BioCyc:   SPEA398579:SPEA_1025-MONOMER
- HAMAP:   MF_01844
- InterPro:   IPR023171
- InterPro:   IPR004670
- TIGRFAMs:   TIGR00773

Pfam domain/function: PF06965 Na_H_antiport_1

EC number: NA

Molecular weight: Translated: 41323; Mature: 41323

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x189bb1d4)-; HASH(0x17bcace0)-; HASH(0x17be70d4)-; HASH(0x192e1de4)-; HASH(0x198620e4)-; HASH(0x18cf8b78)-; HASH(0x18fb151c)-; HASH(0x17a18c8c)-; HASH(0x18e73100)-; HASH(0x17404794)-; HASH(0x1793a914)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERAIRNFLSQESAGGILLMVSVVLAMILANSPLSGMYHGFLETEMQVRVGNLDIDKTLI
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEECCCCHHHHHH
HWINDGLMAIFFMLIGLEVKRELLEGALSSRAQASLPTFAAIGGMVFPAAVYLIFNYSDP
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
ITQVGWAIPAATDIAFALGIMALLGSRVPVALKVFLLALAIIDDLGVVVIIALFYSTDLS
HHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
TISLIIAAAAILGLIGLNRKGVTSLAPYGVVGLILWIAVLKSGVHATLAGVIIAFCIPLR
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
AKDGSSPSESLEHSLHPWSTFIILPIFAFANAGVDLSGMSLSSLLSPVPLGIALGLLVGK
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHCC
PLGIMLFSYIAVKLKLAVLPEGMGWRHITPVAVMCGIGFTMSMFISSLAFVGEGQMYGDY
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ARLGILLGSIASATIGYFWLSKVLPEKGAKA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure
MERAIRNFLSQESAGGILLMVSVVLAMILANSPLSGMYHGFLETEMQVRVGNLDIDKTLI
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEECCCCHHHHHH
HWINDGLMAIFFMLIGLEVKRELLEGALSSRAQASLPTFAAIGGMVFPAAVYLIFNYSDP
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
ITQVGWAIPAATDIAFALGIMALLGSRVPVALKVFLLALAIIDDLGVVVIIALFYSTDLS
HHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
TISLIIAAAAILGLIGLNRKGVTSLAPYGVVGLILWIAVLKSGVHATLAGVIIAFCIPLR
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
AKDGSSPSESLEHSLHPWSTFIILPIFAFANAGVDLSGMSLSSLLSPVPLGIALGLLVGK
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHCC
PLGIMLFSYIAVKLKLAVLPEGMGWRHITPVAVMCGIGFTMSMFISSLAFVGEGQMYGDY
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ARLGILLGSIASATIGYFWLSKVLPEKGAKA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA