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The map label for this gene is ksgA [H]

Identifier: 157960690

GI number: 157960690

Start: 1060129

End: 1060932

Strand: Reverse

Name: ksgA [H]

Synonym: Spea_0862

Alternate gene names: 157960690

Gene position: 1060932-1060129 (Counterclockwise)

Preceding gene: 157960691

Following gene: 157960689

Centisome position: 20.5

GC content: 45.77

Gene sequence:

>804_bases
ATGAGCAATAAAGTACATTTAGGCCACACGGCCAGAAAACGTTTTGGACAAAACTTCTTAACCGACGAAAATGTGATTAA
CCGCATCGTTGGAGCTATTTCACCGGATAACGATCATGTAATGGTCGAAATTGGTCCGGGTCTAGCCGCGCTAACTGAGC
CAGTAGCCAGCGGGATCGACAAGCTAATTGTTGTCGAGCTCGATAAAGATCTGGTTGAGCGTCTAAAAGAGCACCCAGTG
CTTAAAGACAAGCTTGAGATCCATCAAGGTGATGCGCTTAAGTTTGACTTCAATCAGTTAGTGCGTGAAGACAAGCAGAT
GAAGGTGTTTGGTAACCTGCCATATAACATCTCTACACCGCTAATGTTCCACCTATTTGAGTTTGCTCAGCATATTGAAA
ACATGCACTTTATGCTGCAAAAAGAGGTCGTTCTACGCCTCTCTGCTTCACCTGGCACTAAAGCTTATGGTCGTCTAACG
GTCATGGCTCAGTACCATTGCCAAGTGATGCCAGTGCTAGAAGTGCCACCAGGATGTTTCACTCCACCTCCAAAAGTGGA
TTCGGCGGTTGTACGTTTAGTGCCATACAAAGTTAAGCCTTGGCCATGTAAAGATGTGGACTTACTAAGAAACTTAACCA
CGACGGCTTTCAACATGCGCCGTAAAACGCTGAGAAACAACCTTAAACAGTTACTGTCTGACGAAGACTTTGCAGTGCTG
GGTATCGACGCGACACTGAGACCGGAGCAGATCAGTGTTGAGCAATATGTGGCTATGGCTAACCACGTATTCGACAGAAA
GTAA

Upstream 100 bases:

>100_bases
GAGCTTGCAGGCACTGGCACTGCAGATATCGGCAGCTTTGTCTGCGCATTAAATAAAGCCATTGATTTGGCTGCAAAGAA
TTAGTGATAAAAGTTACCTC

Downstream 100 bases:

>100_bases
TCTTATTAATCAAATAAGCTACTGATGAATAAAGGGCATTAACTGCCCTTTTTTTATACCTTTTTTATGGCTTTTTGCCT
ATTAATTCAGATGTTCTCCA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSNKVHLGHTARKRFGQNFLTDENVINRIVGAISPDNDHVMVEIGPGLAALTEPVASGIDKLIVVELDKDLVERLKEHPV
LKDKLEIHQGDALKFDFNQLVREDKQMKVFGNLPYNISTPLMFHLFEFAQHIENMHFMLQKEVVLRLSASPGTKAYGRLT
VMAQYHCQVMPVLEVPPGCFTPPPKVDSAVVRLVPYKVKPWPCKDVDLLRNLTTTAFNMRRKTLRNNLKQLLSDEDFAVL
GIDATLRPEQISVEQYVAMANHVFDRK

Sequences:

>Translated_267_residues
MSNKVHLGHTARKRFGQNFLTDENVINRIVGAISPDNDHVMVEIGPGLAALTEPVASGIDKLIVVELDKDLVERLKEHPV
LKDKLEIHQGDALKFDFNQLVREDKQMKVFGNLPYNISTPLMFHLFEFAQHIENMHFMLQKEVVLRLSASPGTKAYGRLT
VMAQYHCQVMPVLEVPPGCFTPPPKVDSAVVRLVPYKVKPWPCKDVDLLRNLTTTAFNMRRKTLRNNLKQLLSDEDFAVL
GIDATLRPEQISVEQYVAMANHVFDRK
>Mature_266_residues
SNKVHLGHTARKRFGQNFLTDENVINRIVGAISPDNDHVMVEIGPGLAALTEPVASGIDKLIVVELDKDLVERLKEHPVL
KDKLEIHQGDALKFDFNQLVREDKQMKVFGNLPYNISTPLMFHLFEFAQHIENMHFMLQKEVVLRLSASPGTKAYGRLTV
MAQYHCQVMPVLEVPPGCFTPPPKVDSAVVRLVPYKVKPWPCKDVDLLRNLTTTAFNMRRKTLRNNLKQLLSDEDFAVLG
IDATLRPEQISVEQYVAMANHVFDRK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=281, Percent_Identity=29.8932384341637, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI7657198, Length=254, Percent_Identity=31.1023622047244, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1786236, Length=264, Percent_Identity=62.5, Blast_Score=339, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI25141369, Length=282, Percent_Identity=26.9503546099291, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25146882, Length=215, Percent_Identity=33.4883720930233, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=225, Percent_Identity=32, Blast_Score=90, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357273, Length=279, Percent_Identity=30.8243727598566, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21358017, Length=231, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30334; Mature: 30203

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKVHLGHTARKRFGQNFLTDENVINRIVGAISPDNDHVMVEIGPGLAALTEPVASGID
CCCCEECCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCC
KLIVVELDKDLVERLKEHPVLKDKLEIHQGDALKFDFNQLVREDKQMKVFGNLPYNISTP
EEEEEEECHHHHHHHHHCCCCHHHHHHCCCCEEEECHHHHHCCCCCEEEEECCCCCCCCH
LMFHLFEFAQHIENMHFMLQKEVVLRLSASPGTKAYGRLTVMAQYHCQVMPVLEVPPGCF
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHEEEEEEEECEEEEEEEECCCCCC
TPPPKVDSAVVRLVPYKVKPWPCKDVDLLRNLTTTAFNMRRKTLRNNLKQLLSDEDFAVL
CCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
GIDATLRPEQISVEQYVAMANHVFDRK
EECCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNKVHLGHTARKRFGQNFLTDENVINRIVGAISPDNDHVMVEIGPGLAALTEPVASGID
CCCEECCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCC
KLIVVELDKDLVERLKEHPVLKDKLEIHQGDALKFDFNQLVREDKQMKVFGNLPYNISTP
EEEEEEECHHHHHHHHHCCCCHHHHHHCCCCEEEECHHHHHCCCCCEEEEECCCCCCCCH
LMFHLFEFAQHIENMHFMLQKEVVLRLSASPGTKAYGRLTVMAQYHCQVMPVLEVPPGCF
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHEEEEEEEECEEEEEEEECCCCCC
TPPPKVDSAVVRLVPYKVKPWPCKDVDLLRNLTTTAFNMRRKTLRNNLKQLLSDEDFAVL
CCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
GIDATLRPEQISVEQYVAMANHVFDRK
EECCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA