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The map label for this gene is rluE [H]

Identifier: 157960587

GI number: 157960587

Start: 944422

End: 945015

Strand: Reverse

Name: rluE [H]

Synonym: Spea_0759

Alternate gene names: 157960587

Gene position: 945015-944422 (Counterclockwise)

Preceding gene: 157960594

Following gene: 157960583

Centisome position: 18.26

GC content: 49.16

Gene sequence:

>594_bases
ATGTCTTTCATCGAGCAAGCTGCCGAGCAGGCGCATCAACACTTTTTGATCTACAAGCCCTACGGTTTTTTAAGTCAATT
TGTGCCAGAGACCCGAAAAAGTAAGCCGCTACTTGGTCAGCTCTATGACTTTCCCGATAAGACGATGGCTATTGGCCGAC
TCGACCATGACTCTGAAGGCTTGTTGCTACTGACGACTGATGGCATGCAGAGCCACCTCATTCGCAGTAAAAGCATCGAA
AAAGAATATTATGTGCAGGTTGATGGTCTTGTGAGTCAAGCGCAGCTGTTACAGCTACAACAAGGGGTAGAGATCGGCAT
AAGCGGTGGTAAATACCTGACTTTACCCTGTCAGGTAGAACTGCTAACGACTGAACCTAACCTCCCCGCTCGGGGCAAGA
AAATCCGAGATCCTCGCCACGGGCCGACTAGCTGGCTCAGTGTCACCATAGCTGAAGGTAAAAATCGTCAAGTGCGAAAG
ATGACCGCTGCGGTGGGTTTGCCGACCTTAAGGTTAGTTAGAGTCCGAATTGGCCATATTTGTCTTAAACAGATGCAGCC
CGGGGAAGTGCTCCCCGTAAGCAGTTTTATCTAA

Upstream 100 bases:

>100_bases
TGCGGTTGGTATTTTAACACCTCCCCCCTGTTTACCACGACTCAAGCTAAGCGGCTCGATTAGCGATATAATGCTTACTC
CATTACTTTGAGTTTCTACT

Downstream 100 bases:

>100_bases
CTTAAAAGCTATGCGCAACGATTAGTTGCGCGGCGCCTTATGCAAAAAGTCTGTCAGTTCTTCGACTAATTTCGGCTTAC
TATAAAAGTAGCCCTGTGCA

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MSFIEQAAEQAHQHFLIYKPYGFLSQFVPETRKSKPLLGQLYDFPDKTMAIGRLDHDSEGLLLLTTDGMQSHLIRSKSIE
KEYYVQVDGLVSQAQLLQLQQGVEIGISGGKYLTLPCQVELLTTEPNLPARGKKIRDPRHGPTSWLSVTIAEGKNRQVRK
MTAAVGLPTLRLVRVRIGHICLKQMQPGEVLPVSSFI

Sequences:

>Translated_197_residues
MSFIEQAAEQAHQHFLIYKPYGFLSQFVPETRKSKPLLGQLYDFPDKTMAIGRLDHDSEGLLLLTTDGMQSHLIRSKSIE
KEYYVQVDGLVSQAQLLQLQQGVEIGISGGKYLTLPCQVELLTTEPNLPARGKKIRDPRHGPTSWLSVTIAEGKNRQVRK
MTAAVGLPTLRLVRVRIGHICLKQMQPGEVLPVSSFI
>Mature_196_residues
SFIEQAAEQAHQHFLIYKPYGFLSQFVPETRKSKPLLGQLYDFPDKTMAIGRLDHDSEGLLLLTTDGMQSHLIRSKSIEK
EYYVQVDGLVSQAQLLQLQQGVEIGISGGKYLTLPCQVELLTTEPNLPARGKKIRDPRHGPTSWLSVTIAEGKNRQVRKM
TAAVGLPTLRLVRVRIGHICLKQMQPGEVLPVSSFI

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=194, Percent_Identity=40.2061855670103, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1787524, Length=193, Percent_Identity=34.1968911917098, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1788510, Length=193, Percent_Identity=32.6424870466321, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 22063; Mature: 21932

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFIEQAAEQAHQHFLIYKPYGFLSQFVPETRKSKPLLGQLYDFPDKTMAIGRLDHDSEG
CCHHHHHHHHHHHEEEEECCHHHHHHHCCHHCCCCCCHHHHHCCCCCCEEEECCCCCCCC
LLLLTTDGMQSHLIRSKSIEKEYYVQVDGLVSQAQLLQLQQGVEIGISGGKYLTLPCQVE
EEEEEECCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCEEEECCCEEEEEEEEEE
LLTTEPNLPARGKKIRDPRHGPTSWLSVTIAEGKNRQVRKMTAAVGLPTLRLVRVRIGHI
EEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHH
CLKQMQPGEVLPVSSFI
HHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SFIEQAAEQAHQHFLIYKPYGFLSQFVPETRKSKPLLGQLYDFPDKTMAIGRLDHDSEG
CHHHHHHHHHHHEEEEECCHHHHHHHCCHHCCCCCCHHHHHCCCCCCEEEECCCCCCCC
LLLLTTDGMQSHLIRSKSIEKEYYVQVDGLVSQAQLLQLQQGVEIGISGGKYLTLPCQVE
EEEEEECCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCEEEECCCEEEEEEEEEE
LLTTEPNLPARGKKIRDPRHGPTSWLSVTIAEGKNRQVRKMTAAVGLPTLRLVRVRIGHI
EEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHH
CLKQMQPGEVLPVSSFI
HHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA