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The map label for this gene is pcnB [H]

Identifier: 157960524

GI number: 157960524

Start: 855987

End: 857291

Strand: Reverse

Name: pcnB [H]

Synonym: Spea_0696

Alternate gene names: 157960524

Gene position: 857291-855987 (Counterclockwise)

Preceding gene: 157960525

Following gene: 157960523

Centisome position: 16.57

GC content: 48.12

Gene sequence:

>1305_bases
GTGCCTAGAAATGCGCACGCTATTTCTCGCAGACAGATCAGTGAAAATGCGATCAAAGTACTCTATAGACTACATAAGTC
AGGCTTTAAAGCCTACCTAGTCGGTGGCGGTGTGCGCGATATCTTATTGGGTTTACAACCGAAAGATTTCGATGTCGTGA
CCAATGCAACGCCCGAAGAGATTAAGCGACTGTTCCGTAACTGCCGCCTCGTAGGCCGTCGTTTTCGTCTCGCTCATATC
GTTTTTGGACGCGATGTGATCGAAGTTGCGACCCTGCGTGGACACCACGTCGATAGCGGCGAAAAGATCTCTAAGACCAA
TGAATCGGGTCGCCTGCTGCGTGACAATGTCTACGGCAGCATAGATGAAGACGCTGAACGTCGTGACTTTACCGTCAACG
CGCTATATTACGATATTAGTGACTTCTCTATTCACAGCTATGGCGGTGGACTACAAGATTTAGAATCGCGCACTATCCGC
TTGATTGGTGACCCAGAGACTCGCTACCGTGAAGACCCTGTGCGTATGCTTCGTGCAGTGCGTTTCGCAACTAAGCTGGG
GATGAGCATTGAACCTAAGGCCGCAGAGCCAATTCAAGGCCTAGCACACCTGCTTAAAGATATTCCTGCCGCGCGTATGT
ATGAAGAAGTATTGAAGCTGTTTTTCGCAGGAAAAGCGCTTGAAAACTACCATATGATGCGAGAATTTGGTCTATTTGAG
CCACTATTCCCTCAGCTTGAAGCACTGTTAAAAGAATATCCAAACGGTGAAACGGCTAAGTTATTACAAGAGATGATGGC
AAATACCGATGAGCGCGTAGCGCAAGATAAGCCGGTTACACCAGCCTTCTTCTATGCAGCATTACTTTGGTACCCATTGA
GCCAGCGCGCAGACGATATCGCACTGGAAAGTGGCCTAAGCTCTTACGACGCTTATAATGCCGCCATGGGTGATGTCATG
GAGCAGCAATGCCGAACCATTAGCGTTCCGCGCCGCTTTAGCACTATCACTAAAGATATTTGGCAGCTACAACTTAGGTT
CGAGCGAAACAGAGGCACTAAGGCCTTTAGATTTATCGAACATCCTAAGTTTAGAGCCGCATACGATCTGTTATTGCTAC
GAGCCCATGCAGAAGGTGGTGCTTTAGCTAAGAGTGCTGCTTGGTGGAAGAGCTTTGTTGAAGGCAATGAAGATCAACGT
AATGTGATTTCTCGCTCTACCAATAAAGGTAGTCGTAACCGCAGCTCTAACCAACGTCGCCGCCGTAAGCCAAGCGCTAA
ATCGGCACCTAAATCTGCTGAATAA

Upstream 100 bases:

>100_bases
GTGTCCCATTTTTCGCCGTATTAGCCAATTCTGTAAACAGCTTTTTGAAGATAGTCCAAAAGCTGAAGAGCACACACAGA
CAGGTCTTAGTTTAGAGGTC

Downstream 100 bases:

>100_bases
TGGCGCAAGTCTATGTCGCTTTGGGCGCTAACTTAGCGGACCCAATTAGCCAGCTCAATAATGCCTGTGAGGCATTGTTG
AGCTTGGCGCATGCAGATTC

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MPRNAHAISRRQISENAIKVLYRLHKSGFKAYLVGGGVRDILLGLQPKDFDVVTNATPEEIKRLFRNCRLVGRRFRLAHI
VFGRDVIEVATLRGHHVDSGEKISKTNESGRLLRDNVYGSIDEDAERRDFTVNALYYDISDFSIHSYGGGLQDLESRTIR
LIGDPETRYREDPVRMLRAVRFATKLGMSIEPKAAEPIQGLAHLLKDIPAARMYEEVLKLFFAGKALENYHMMREFGLFE
PLFPQLEALLKEYPNGETAKLLQEMMANTDERVAQDKPVTPAFFYAALLWYPLSQRADDIALESGLSSYDAYNAAMGDVM
EQQCRTISVPRRFSTITKDIWQLQLRFERNRGTKAFRFIEHPKFRAAYDLLLLRAHAEGGALAKSAAWWKSFVEGNEDQR
NVISRSTNKGSRNRSSNQRRRRKPSAKSAPKSAE

Sequences:

>Translated_434_residues
MPRNAHAISRRQISENAIKVLYRLHKSGFKAYLVGGGVRDILLGLQPKDFDVVTNATPEEIKRLFRNCRLVGRRFRLAHI
VFGRDVIEVATLRGHHVDSGEKISKTNESGRLLRDNVYGSIDEDAERRDFTVNALYYDISDFSIHSYGGGLQDLESRTIR
LIGDPETRYREDPVRMLRAVRFATKLGMSIEPKAAEPIQGLAHLLKDIPAARMYEEVLKLFFAGKALENYHMMREFGLFE
PLFPQLEALLKEYPNGETAKLLQEMMANTDERVAQDKPVTPAFFYAALLWYPLSQRADDIALESGLSSYDAYNAAMGDVM
EQQCRTISVPRRFSTITKDIWQLQLRFERNRGTKAFRFIEHPKFRAAYDLLLLRAHAEGGALAKSAAWWKSFVEGNEDQR
NVISRSTNKGSRNRSSNQRRRRKPSAKSAPKSAE
>Mature_433_residues
PRNAHAISRRQISENAIKVLYRLHKSGFKAYLVGGGVRDILLGLQPKDFDVVTNATPEEIKRLFRNCRLVGRRFRLAHIV
FGRDVIEVATLRGHHVDSGEKISKTNESGRLLRDNVYGSIDEDAERRDFTVNALYYDISDFSIHSYGGGLQDLESRTIRL
IGDPETRYREDPVRMLRAVRFATKLGMSIEPKAAEPIQGLAHLLKDIPAARMYEEVLKLFFAGKALENYHMMREFGLFEP
LFPQLEALLKEYPNGETAKLLQEMMANTDERVAQDKPVTPAFFYAALLWYPLSQRADDIALESGLSSYDAYNAAMGDVME
QQCRTISVPRRFSTITKDIWQLQLRFERNRGTKAFRFIEHPKFRAAYDLLLLRAHAEGGALAKSAAWWKSFVEGNEDQRN
VISRSTNKGSRNRSSNQRRRRKPSAKSAPKSAE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=212, Percent_Identity=28.3018867924528, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI87081691, Length=408, Percent_Identity=56.1274509803922, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1789436, Length=228, Percent_Identity=30.7017543859649, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=225, Percent_Identity=29.3333333333333, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321016, Length=186, Percent_Identity=27.4193548387097, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281360112, Length=273, Percent_Identity=26.007326007326, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21357337, Length=273, Percent_Identity=26.007326007326, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 49561; Mature: 49430

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRNAHAISRRQISENAIKVLYRLHKSGFKAYLVGGGVRDILLGLQPKDFDVVTNATPEE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCCEECCCCHHH
IKRLFRNCRLVGRRFRLAHIVFGRDVIEVATLRGHHVDSGEKISKTNESGRLLRDNVYGS
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHCCCCCCCEEHHCCCCC
IDEDAERRDFTVNALYYDISDFSIHSYGGGLQDLESRTIRLIGDPETRYREDPVRMLRAV
CCCCCCCCCEEEEEEEEECCCCEECCCCCCHHHHCCCEEEEECCCHHHHCCCHHHHHHHH
RFATKLGMSIEPKAAEPIQGLAHLLKDIPAARMYEEVLKLFFAGKALENYHMMREFGLFE
HHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLFPQLEALLKEYPNGETAKLLQEMMANTDERVAQDKPVTPAFFYAALLWYPLSQRADDI
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHH
ALESGLSSYDAYNAAMGDVMEQQCRTISVPRRFSTITKDIWQLQLRFERNRGTKAFRFIE
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHC
HPKFRAAYDLLLLRAHAEGGALAKSAAWWKSFVEGNEDQRNVISRSTNKGSRNRSSNQRR
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHHH
RRKPSAKSAPKSAE
HCCCCCCCCCCCCC
>Mature Secondary Structure 
PRNAHAISRRQISENAIKVLYRLHKSGFKAYLVGGGVRDILLGLQPKDFDVVTNATPEE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCCEECCCCHHH
IKRLFRNCRLVGRRFRLAHIVFGRDVIEVATLRGHHVDSGEKISKTNESGRLLRDNVYGS
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHCCCCCCCEEHHCCCCC
IDEDAERRDFTVNALYYDISDFSIHSYGGGLQDLESRTIRLIGDPETRYREDPVRMLRAV
CCCCCCCCCEEEEEEEEECCCCEECCCCCCHHHHCCCEEEEECCCHHHHCCCHHHHHHHH
RFATKLGMSIEPKAAEPIQGLAHLLKDIPAARMYEEVLKLFFAGKALENYHMMREFGLFE
HHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLFPQLEALLKEYPNGETAKLLQEMMANTDERVAQDKPVTPAFFYAALLWYPLSQRADDI
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHH
ALESGLSSYDAYNAAMGDVMEQQCRTISVPRRFSTITKDIWQLQLRFERNRGTKAFRFIE
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHC
HPKFRAAYDLLLLRAHAEGGALAKSAAWWKSFVEGNEDQRNVISRSTNKGSRNRSSNQRR
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHHH
RRKPSAKSAPKSAE
HCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]