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The map label for this gene is pntA [H]

Identifier: 157960480

GI number: 157960480

Start: 806523

End: 807752

Strand: Reverse

Name: pntA [H]

Synonym: Spea_0652

Alternate gene names: 157960480

Gene position: 807752-806523 (Counterclockwise)

Preceding gene: 157960487

Following gene: 157960479

Centisome position: 15.61

GC content: 49.27

Gene sequence:

>1230_bases
ATGACATCAATCCCTACGATTGTCCCAATAGAGAGGTTTACCTTTGTGAAGCTAGGTCTACCCAAAGAGAGTTATGCTAG
TGAAAGTCGCGTCGCGATTATTCCAGCCAATGTGACTCGACTACTTAAAATTAACCTGCAAGTTCTTGTTGAAAGTGGCG
CTGGTAGTAATGCTGGCTTTACCGATGAAGACTATATTCAGGCCGGCGCAAGCATAGTGTCTCGTGATCAGCTCTTCAGC
GATGCGGACATTATTACCTTAGTGAATGCTAAAGATGAGCAGCTTAACGAAATCAAGTCCAAGCTTAAGCCACAACAGAT
CGTGATCGGCATGATGGATCCATTAGCGCATCCAGAACACGCCCAAACCTTTGCCGAAACTGGGGCTACAGCCATTGCCC
TAGAACTTGTGCCACGGATCACCCGCGCTCAGAGTATGGATATTCTGTCTTCTATGGCGACAATCGCTGGCTATAAAGCT
GTATTGCTAGCGGCCCATAAAGCCCCACGGTTATTTCCTATGATGATGACAGCAGCGGGTACCTTAAAACCTGCACGCGC
CTTTATCATGGGTGTGGGTGTTGCCGGACTGCAAGCTTGTGCGACCGCCAAACGCTTAGGTGCTGTAGTCGAAGCCTATG
ATATTCGCCCTGCAGCGCGCGAGCAGATCATCTCTGTTGGTGCCAAGCCTGTTGAACTTGACTTAGAAGCGGAAAACACC
GAAACCAAAGGTGGCTACGCGACCGAGCAGTCTGATGACTTCCTTAAGCGTCAACAACAAGCCATGGCGAACGTGCTTGC
TCAGCAAGATATCGTGATCACCACTGCCGCTATTCCTGGTCGTAAGGCCCCTATCCTTATCACTAAGGAGATGGTTGATG
GCATGAAGAACGGCACCATTATTGTCGATCTCGCCGCAGAAACCGGTGGTAACTGTGAGCTAACCGAACTCGATAAAGAG
GTCGTTTACAAAGGTGTGACCATACTCGGGCCATCCAATGTACCGGGCACTGCGGCTAATCACGCCAGCCAGATGTACGG
CACTAACATCGAAAACTTGCTTAAGTTACTCGTCAGTAAAGAAGGCGAGCTTAATCTCGATTTTGAAGATGAGATCATTA
GAGACACGGTTGTTGCCCACAAAGGTGAAGTCGTGAGCACACGTTTACGCGAGCTTTTAGGCTTGCCAGCGCTTGAAGTG
ACAAGCGAAACGTCACCGGAGGCAAAATAA

Upstream 100 bases:

>100_bases
CGCAAACTTTTCAGTCTGACCAGTCTGGAAAAACCTATATTGAAAAATATACGCTTGTGTCTAAATCTAGAGTCGCCACA
ATATGCACCCTAACCAATAA

Downstream 100 bases:

>100_bases
TGGAAATTTTACTATTACTGACCCTGTTCGTGGTCGCGGTATTCTTAGGCGTCGAGATGATCACTAAGGTACCCCCAACG
CTACATACACCACTGATGTC

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTSIPTIVPIERFTFVKLGLPKESYASESRVAIIPANVTRLLKINLQVLVESGAGSNAGFTDEDYIQAGASIVSRDQLFS
DADIITLVNAKDEQLNEIKSKLKPQQIVIGMMDPLAHPEHAQTFAETGATAIALELVPRITRAQSMDILSSMATIAGYKA
VLLAAHKAPRLFPMMMTAAGTLKPARAFIMGVGVAGLQACATAKRLGAVVEAYDIRPAAREQIISVGAKPVELDLEAENT
ETKGGYATEQSDDFLKRQQQAMANVLAQQDIVITTAAIPGRKAPILITKEMVDGMKNGTIIVDLAAETGGNCELTELDKE
VVYKGVTILGPSNVPGTAANHASQMYGTNIENLLKLLVSKEGELNLDFEDEIIRDTVVAHKGEVVSTRLRELLGLPALEV
TSETSPEAK

Sequences:

>Translated_409_residues
MTSIPTIVPIERFTFVKLGLPKESYASESRVAIIPANVTRLLKINLQVLVESGAGSNAGFTDEDYIQAGASIVSRDQLFS
DADIITLVNAKDEQLNEIKSKLKPQQIVIGMMDPLAHPEHAQTFAETGATAIALELVPRITRAQSMDILSSMATIAGYKA
VLLAAHKAPRLFPMMMTAAGTLKPARAFIMGVGVAGLQACATAKRLGAVVEAYDIRPAAREQIISVGAKPVELDLEAENT
ETKGGYATEQSDDFLKRQQQAMANVLAQQDIVITTAAIPGRKAPILITKEMVDGMKNGTIIVDLAAETGGNCELTELDKE
VVYKGVTILGPSNVPGTAANHASQMYGTNIENLLKLLVSKEGELNLDFEDEIIRDTVVAHKGEVVSTRLRELLGLPALEV
TSETSPEAK
>Mature_408_residues
TSIPTIVPIERFTFVKLGLPKESYASESRVAIIPANVTRLLKINLQVLVESGAGSNAGFTDEDYIQAGASIVSRDQLFSD
ADIITLVNAKDEQLNEIKSKLKPQQIVIGMMDPLAHPEHAQTFAETGATAIALELVPRITRAQSMDILSSMATIAGYKAV
LLAAHKAPRLFPMMMTAAGTLKPARAFIMGVGVAGLQACATAKRLGAVVEAYDIRPAAREQIISVGAKPVELDLEAENTE
TKGGYATEQSDDFLKRQQQAMANVLAQQDIVITTAAIPGRKAPILITKEMVDGMKNGTIIVDLAAETGGNCELTELDKEV
VYKGVTILGPSNVPGTAANHASQMYGTNIENLLKLLVSKEGELNLDFEDEIIRDTVVAHKGEVVSTRLRELLGLPALEVT
SETSPEAK

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=389, Percent_Identity=40.6169665809769, Blast_Score=274, Evalue=9e-74,
Organism=Homo sapiens, GI122939153, Length=389, Percent_Identity=40.6169665809769, Blast_Score=274, Evalue=9e-74,
Organism=Escherichia coli, GI1787887, Length=372, Percent_Identity=44.8924731182796, Blast_Score=308, Evalue=6e-85,
Organism=Caenorhabditis elegans, GI17550456, Length=372, Percent_Identity=39.247311827957, Blast_Score=258, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 43774; Mature: 43643

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIPTIVPIERFTFVKLGLPKESYASESRVAIIPANVTRLLKINLQVLVESGAGSNAGF
CCCCCCEEECCCEEEEEECCCCHHHCCCCEEEEEECCCEEEHEEEEEEEEECCCCCCCCC
TDEDYIQAGASIVSRDQLFSDADIITLVNAKDEQLNEIKSKLKPQQIVIGMMDPLAHPEH
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCHHEEEEHHCCCCCCHH
AQTFAETGATAIALELVPRITRAQSMDILSSMATIAGYKAVLLAAHKAPRLFPMMMTAAG
HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
TLKPARAFIMGVGVAGLQACATAKRLGAVVEAYDIRPAAREQIISVGAKPVELDLEAENT
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCC
ETKGGYATEQSDDFLKRQQQAMANVLAQQDIVITTAAIPGRKAPILITKEMVDGMKNGTI
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHCCCCCCEE
IVDLAAETGGNCELTELDKEVVYKGVTILGPSNVPGTAANHASQMYGTNIENLLKLLVSK
EEEEECCCCCCEEHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHCC
EGELNLDFEDEIIRDTVVAHKGEVVSTRLRELLGLPALEVTSETSPEAK
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCCC
>Mature Secondary Structure 
TSIPTIVPIERFTFVKLGLPKESYASESRVAIIPANVTRLLKINLQVLVESGAGSNAGF
CCCCCEEECCCEEEEEECCCCHHHCCCCEEEEEECCCEEEHEEEEEEEEECCCCCCCCC
TDEDYIQAGASIVSRDQLFSDADIITLVNAKDEQLNEIKSKLKPQQIVIGMMDPLAHPEH
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCHHEEEEHHCCCCCCHH
AQTFAETGATAIALELVPRITRAQSMDILSSMATIAGYKAVLLAAHKAPRLFPMMMTAAG
HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
TLKPARAFIMGVGVAGLQACATAKRLGAVVEAYDIRPAAREQIISVGAKPVELDLEAENT
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCC
ETKGGYATEQSDDFLKRQQQAMANVLAQQDIVITTAAIPGRKAPILITKEMVDGMKNGTI
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHCCCCCCEE
IVDLAAETGGNCELTELDKEVVYKGVTILGPSNVPGTAANHASQMYGTNIENLLKLLVSK
EEEEECCCCCCEEHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHCC
EGELNLDFEDEIIRDTVVAHKGEVVSTRLRELLGLPALEVTSETSPEAK
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]