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The map label for this gene is htrB [H]

Identifier: 157960473

GI number: 157960473

Start: 798736

End: 799662

Strand: Reverse

Name: htrB [H]

Synonym: Spea_0645

Alternate gene names: 157960473

Gene position: 799662-798736 (Counterclockwise)

Preceding gene: 157960477

Following gene: 157960468

Centisome position: 15.45

GC content: 48.33

Gene sequence:

>927_bases
GTGGTAGAAAAAGCTCAATTTTCATCTCACCTTTATCATCCCAAGTACTGGCTCTTATGGTTAGGCGTGGCTTTTATGCG
GTTAACTCAACTGTTACCACTGCCAGTCCAAATGAAGATAGGCGCCGCGATTGGCCGTTTAGGCATGAAGCTAGTAGGCA
AACGAGTTAAGACCGCCAAACGTAATCTAGAGCTGTGTTTTCCGAAAATGTCTGAAAATGAGATGCAGCATCTGCTCACG
CAAAACTTCGAGCAAACCGGTAAAGCCATCTTCGACACGGCCAACGCCTGGTGGTGGTCTGATGAAAAAATCCAGCAGCA
CATGAGCATTAAAGGCAAAGAGTATGTTGAACAGACTCTAGAGAGTGGCCAAGGTGTGATTTTGTTTGCGGTGCACTGCC
TGCCCCTTGAGATGGGAGCCCGAATTTTCGGACAGTTCCAACCCGGTGTTGGAGTGTATCGCCCCCACAATAACCCCGTG
ATGGAGTACCTACAGGTTAAAGGTCGCCTGCGTTCCAACAAGGCCTTAGTGCCAAAACGCGATCTACGCCAGATGGTACG
CAGTCTGCGCGAGCCTAATGTGATCTGGTACACCGCCGACCAAGATTTTGGCCGCTCAAGCGCCACCTTCATCCCTTTCT
TCAATGTTGCCGAAGCCGCGACTATCACCGGTGCCACCACCCTCGCCAAGTTAGGCAAAGCCAAAGTGTTACCTTTCTTC
GTCGAACGTAATAGTGATGACACCGGCTACAATATTGAGATCCAACCGGCTTTGGATAATTTCCCTGGCGAGAATGAAAT
TGAAGATGCCAAGCGCGGAAATAAGATCATTGAATCTATTATTAGCCGCAATATGTCGCAGTATATGTGGTTACATAGAC
GGTTTAAGACACGTCCGAATAAAGGCGATGCTTCGTTGTATAAATAG

Upstream 100 bases:

>100_bases
AACGCCTATATCTATCACAAAATTTAATCCTGATTGTACCTAATTTTAATCACTTTTGAGTTAGAATAATGAGTAATTTT
TCAATGGATCCGAGAATTAC

Downstream 100 bases:

>100_bases
GTTCTAGGTTCTAGGTTCTAGGTTCTAGGTTCTAGGTTCTAGGTTCTAGGTTCTAGGTTCTAGGTTCTAGCATAGCTAAA
GATATCAGGCTGCGCCTGAC

Product: lipid A biosynthesis lauroyl (or palmitoleoyl) acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MVEKAQFSSHLYHPKYWLLWLGVAFMRLTQLLPLPVQMKIGAAIGRLGMKLVGKRVKTAKRNLELCFPKMSENEMQHLLT
QNFEQTGKAIFDTANAWWWSDEKIQQHMSIKGKEYVEQTLESGQGVILFAVHCLPLEMGARIFGQFQPGVGVYRPHNNPV
MEYLQVKGRLRSNKALVPKRDLRQMVRSLREPNVIWYTADQDFGRSSATFIPFFNVAEAATITGATTLAKLGKAKVLPFF
VERNSDDTGYNIEIQPALDNFPGENEIEDAKRGNKIIESIISRNMSQYMWLHRRFKTRPNKGDASLYK

Sequences:

>Translated_308_residues
MVEKAQFSSHLYHPKYWLLWLGVAFMRLTQLLPLPVQMKIGAAIGRLGMKLVGKRVKTAKRNLELCFPKMSENEMQHLLT
QNFEQTGKAIFDTANAWWWSDEKIQQHMSIKGKEYVEQTLESGQGVILFAVHCLPLEMGARIFGQFQPGVGVYRPHNNPV
MEYLQVKGRLRSNKALVPKRDLRQMVRSLREPNVIWYTADQDFGRSSATFIPFFNVAEAATITGATTLAKLGKAKVLPFF
VERNSDDTGYNIEIQPALDNFPGENEIEDAKRGNKIIESIISRNMSQYMWLHRRFKTRPNKGDASLYK
>Mature_308_residues
MVEKAQFSSHLYHPKYWLLWLGVAFMRLTQLLPLPVQMKIGAAIGRLGMKLVGKRVKTAKRNLELCFPKMSENEMQHLLT
QNFEQTGKAIFDTANAWWWSDEKIQQHMSIKGKEYVEQTLESGQGVILFAVHCLPLEMGARIFGQFQPGVGVYRPHNNPV
MEYLQVKGRLRSNKALVPKRDLRQMVRSLREPNVIWYTADQDFGRSSATFIPFFNVAEAATITGATTLAKLGKAKVLPFF
VERNSDDTGYNIEIQPALDNFPGENEIEDAKRGNKIIESIISRNMSQYMWLHRRFKTRPNKGDASLYK

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=307, Percent_Identity=41.3680781758958, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI87082094, Length=307, Percent_Identity=38.7622149837134, Blast_Score=258, Evalue=5e-70,
Organism=Escherichia coli, GI1788161, Length=305, Percent_Identity=28.1967213114754, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35339; Mature: 35339

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKAQFSSHLYHPKYWLLWLGVAFMRLTQLLPLPVQMKIGAAIGRLGMKLVGKRVKTAK
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RNLELCFPKMSENEMQHLLTQNFEQTGKAIFDTANAWWWSDEKIQQHMSIKGKEYVEQTL
CCHHEECCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHH
ESGQGVILFAVHCLPLEMGARIFGQFQPGVGVYRPHNNPVMEYLQVKGRLRSNKALVPKR
HCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCHH
DLRQMVRSLREPNVIWYTADQDFGRSSATFIPFFNVAEAATITGATTLAKLGKAKVLPFF
HHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEE
VERNSDDTGYNIEIQPALDNFPGENEIEDAKRGNKIIESIISRNMSQYMWLHRRFKTRPN
EECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
KGDASLYK
CCCCCCCC
>Mature Secondary Structure
MVEKAQFSSHLYHPKYWLLWLGVAFMRLTQLLPLPVQMKIGAAIGRLGMKLVGKRVKTAK
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RNLELCFPKMSENEMQHLLTQNFEQTGKAIFDTANAWWWSDEKIQQHMSIKGKEYVEQTL
CCHHEECCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHH
ESGQGVILFAVHCLPLEMGARIFGQFQPGVGVYRPHNNPVMEYLQVKGRLRSNKALVPKR
HCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCHH
DLRQMVRSLREPNVIWYTADQDFGRSSATFIPFFNVAEAATITGATTLAKLGKAKVLPFF
HHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEE
VERNSDDTGYNIEIQPALDNFPGENEIEDAKRGNKIIESIISRNMSQYMWLHRRFKTRPN
EECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
KGDASLYK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800; 7592970 [H]