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The map label for this gene is napB [H]

Identifier: 157960449

GI number: 157960449

Start: 763871

End: 764296

Strand: Reverse

Name: napB [H]

Synonym: Spea_0620

Alternate gene names: 157960449

Gene position: 764296-763871 (Counterclockwise)

Preceding gene: 157960450

Following gene: 157960448

Centisome position: 14.77

GC content: 49.3

Gene sequence:

>426_bases
ATGAAAAAAGTACTCACCTTAGCTGCGCTAGTGATGGCGCTTAGTGCTTGCTCTGGTCAGCAAGCGAACACTCAAGCCGA
GCCTGTTAACATCAGCTCACTCGGTAAGTCTGAGATAACCGATATTCGCGCAGCAGATCCTGCACCGCTTTATCCAAAGC
GCGGTAAGTCTATCGAGCGTACCTTTGTACATCAGCCACCTATGATCCCGCATAAAGCCGATTACCCAATCACGCTTAAG
CGCAACGGCTGTATCAGCTGCCACAGCCCAGCAAAAGCTAAGCGTATGAAGGCAACGGCTGTGGATCCATCGCATATGTT
GGCCGACGGCAAGCTAAACAACGAGTACTACAACTGTACTCAGTGTCACACCCCGCAAGCTGAAAACAAGCAGCACTTAG
TTGAAAACGAATTCTCAACTAACTAA

Upstream 100 bases:

>100_bases
CTCAATGCGGCCGCTGTATCGACGTTTGCGCCCAACGCGTTTTCCAGTTTCAGAATCGAAAGTATCAAAATCGAATTGCC
CTAAAGGCGGAGAACAACCA

Downstream 100 bases:

>100_bases
TCCCGATAAGCGGCTTGTTTAAGCTAAAAAAAACCACCTCATTTGAGGTGGTTTTTTATTTTGTTCACGCAGAAATTATT
ATTCTAAAGGCGCTAATAGC

Product: nitrate reductase cytochrome c-type subunit NapB

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 141; Mature: 141

Protein sequence:

>141_residues
MKKVLTLAALVMALSACSGQQANTQAEPVNISSLGKSEITDIRAADPAPLYPKRGKSIERTFVHQPPMIPHKADYPITLK
RNGCISCHSPAKAKRMKATAVDPSHMLADGKLNNEYYNCTQCHTPQAENKQHLVENEFSTN

Sequences:

>Translated_141_residues
MKKVLTLAALVMALSACSGQQANTQAEPVNISSLGKSEITDIRAADPAPLYPKRGKSIERTFVHQPPMIPHKADYPITLK
RNGCISCHSPAKAKRMKATAVDPSHMLADGKLNNEYYNCTQCHTPQAENKQHLVENEFSTN
>Mature_141_residues
MKKVLTLAALVMALSACSGQQANTQAEPVNISSLGKSEITDIRAADPAPLYPKRGKSIERTFVHQPPMIPHKADYPITLK
RNGCISCHSPAKAKRMKATAVDPSHMLADGKLNNEYYNCTQCHTPQAENKQHLVENEFSTN

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=94, Percent_Identity=36.1702127659575, Blast_Score=63, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 15472; Mature: 15472

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLTLAALVMALSACSGQQANTQAEPVNISSLGKSEITDIRAADPAPLYPKRGKSIER
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCHHCCCCCCCCCCCCCCCCCCCCH
TFVHQPPMIPHKADYPITLKRNGCISCHSPAKAKRMKATAVDPSHMLADGKLNNEYYNCT
HCCCCCCCCCCCCCCCEEEECCCCEECCCCCHHHHEECCCCCHHHHEECCCCCCCEECCC
QCHTPQAENKQHLVENEFSTN
CCCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MKKVLTLAALVMALSACSGQQANTQAEPVNISSLGKSEITDIRAADPAPLYPKRGKSIER
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCHHCCCCCCCCCCCCCCCCCCCCH
TFVHQPPMIPHKADYPITLKRNGCISCHSPAKAKRMKATAVDPSHMLADGKLNNEYYNCT
HCCCCCCCCCCCCCCCEEEECCCCEECCCCCHHHHEECCCCCHHHHEECCCCCCCEECCC
QCHTPQAENKQHLVENEFSTN
CCCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 11389694; 11223519 [H]