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The map label for this gene is gshB [H]

Identifier: 157960428

GI number: 157960428

Start: 736964

End: 737917

Strand: Reverse

Name: gshB [H]

Synonym: Spea_0599

Alternate gene names: 157960428

Gene position: 737917-736964 (Counterclockwise)

Preceding gene: 157960429

Following gene: 157960427

Centisome position: 14.26

GC content: 47.9

Gene sequence:

>954_bases
ATGATCAAACTCGGCATCGTGATGGATCCCATCAGCGACATTAATATCAAGAAAGATTCTAGCTTTGCCATGTTAATGGC
TGCCCAGTCTCGTGGTTATCAGCTGTTCTACATGGAGATGAAAGATCTTGCCATGGTCAACGGCCAAGCGATGGCAACCA
TGCGCGCATTAACAGTGAAAAATGATCCACAAGAGTGGTACCAGCTTGAAGAAGCCGTAGATACTCCGCTTGCCGACTTG
GACGTGATCTTAATGCGCAAGGATCCGCCGTTCGACACTGAATTCGTCTATTCAACTTACATGCTTGAGCGCGCTGAAGA
GCAAGGCGTGTTAATTGTAAACAAACCACAAAGCCTGCGTGATGCCAACGAAAAACTATTTACCGCTTGGTTCTCTGAGT
TCACCCCAGAAACCATAGTCACCCGTGACGAGCAACGCATTCGCGCTTTCCACCAAGCCAAAGGCGATATCATCCTTAAG
CCATTAGATGGTATGGGTGGCAGCTCTATTTTCAGAGTGAAAAAAGATGATCCAAACCTAGGGGTTATCATCGAGACACT
TACCGCTGAAGGCTCGCAATACGCGATGGCGCAAGCCTTTATCCCTGATATTACTGCAGGCGACAAGCGCATCTTAGTGG
TTGATGGTGAGCCAGTGCCTTACTCATTAGCCCGTATTCCACAAAAAGGTGAAACCCGTGGCAATTTAGCCGCTGGCGGC
CGTGGTGTAGCCCAGCCTCTATCTGAGTCTGATTGGCACATTGCGCGTACGATTGGCCCCGAGCTTAAAAAACGCGGTCT
TATCTTTGTTGGCTTAGATGTGATTGGCGACAAGCTTACCGAGATCAACGTCACTAGCCCTACCTGTATTAAAGAGATTG
AAGCAGCCTTCGATGTCGATATCACAGGCATGTTAATGGATGCAATTGAACAGCGTATCAACAAATCAGCATAG

Upstream 100 bases:

>100_bases
ACTCTGCCAAGTAGAAGATTTATTAGTAGCACGCTAGAAAACGGGGGCTTATGCCCCCATATTTATCGTTGAAACGAATA
TAAGATAAGGAAACCGCTCC

Downstream 100 bases:

>100_bases
CCAAAGGATTCGGCATGAACTTGAGAAAAACATGCACTTGGGCACTTAGTGCCCTTGTTGTGTTACCACTATTTGCATCG
GCCAATATCGATATTCATGA

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MIKLGIVMDPISDINIKKDSSFAMLMAAQSRGYQLFYMEMKDLAMVNGQAMATMRALTVKNDPQEWYQLEEAVDTPLADL
DVILMRKDPPFDTEFVYSTYMLERAEEQGVLIVNKPQSLRDANEKLFTAWFSEFTPETIVTRDEQRIRAFHQAKGDIILK
PLDGMGGSSIFRVKKDDPNLGVIIETLTAEGSQYAMAQAFIPDITAGDKRILVVDGEPVPYSLARIPQKGETRGNLAAGG
RGVAQPLSESDWHIARTIGPELKKRGLIFVGLDVIGDKLTEINVTSPTCIKEIEAAFDVDITGMLMDAIEQRINKSA

Sequences:

>Translated_317_residues
MIKLGIVMDPISDINIKKDSSFAMLMAAQSRGYQLFYMEMKDLAMVNGQAMATMRALTVKNDPQEWYQLEEAVDTPLADL
DVILMRKDPPFDTEFVYSTYMLERAEEQGVLIVNKPQSLRDANEKLFTAWFSEFTPETIVTRDEQRIRAFHQAKGDIILK
PLDGMGGSSIFRVKKDDPNLGVIIETLTAEGSQYAMAQAFIPDITAGDKRILVVDGEPVPYSLARIPQKGETRGNLAAGG
RGVAQPLSESDWHIARTIGPELKKRGLIFVGLDVIGDKLTEINVTSPTCIKEIEAAFDVDITGMLMDAIEQRINKSA
>Mature_317_residues
MIKLGIVMDPISDINIKKDSSFAMLMAAQSRGYQLFYMEMKDLAMVNGQAMATMRALTVKNDPQEWYQLEEAVDTPLADL
DVILMRKDPPFDTEFVYSTYMLERAEEQGVLIVNKPQSLRDANEKLFTAWFSEFTPETIVTRDEQRIRAFHQAKGDIILK
PLDGMGGSSIFRVKKDDPNLGVIIETLTAEGSQYAMAQAFIPDITAGDKRILVVDGEPVPYSLARIPQKGETRGNLAAGG
RGVAQPLSESDWHIARTIGPELKKRGLIFVGLDVIGDKLTEINVTSPTCIKEIEAAFDVDITGMLMDAIEQRINKSA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=315, Percent_Identity=72.6984126984127, Blast_Score=484, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35223; Mature: 35223

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLGIVMDPISDINIKKDSSFAMLMAAQSRGYQLFYMEMKDLAMVNGQAMATMRALTVK
CEEEEEEECCCCCCCCCCCCCEEEEEEECCCCEEEEEEEEHHHHHCCCCHHEEEEEEEEC
NDPQEWYQLEEAVDTPLADLDVILMRKDPPFDTEFVYSTYMLERAEEQGVLIVNKPQSLR
CCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHH
DANEKLFTAWFSEFTPETIVTRDEQRIRAFHQAKGDIILKPLDGMGGSSIFRVKKDDPNL
HHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCC
GVIIETLTAEGSQYAMAQAFIPDITAGDKRILVVDGEPVPYSLARIPQKGETRGNLAAGG
EEEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCCC
RGVAQPLSESDWHIARTIGPELKKRGLIFVGLDVIGDKLTEINVTSPTCIKEIEAAFDVD
CCCCCCCCCCCCEEEECCCHHHHHCCEEEEEHHHHCCCEEEEECCCCHHHHHHHHHHCCC
ITGMLMDAIEQRINKSA
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKLGIVMDPISDINIKKDSSFAMLMAAQSRGYQLFYMEMKDLAMVNGQAMATMRALTVK
CEEEEEEECCCCCCCCCCCCCEEEEEEECCCCEEEEEEEEHHHHHCCCCHHEEEEEEEEC
NDPQEWYQLEEAVDTPLADLDVILMRKDPPFDTEFVYSTYMLERAEEQGVLIVNKPQSLR
CCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHH
DANEKLFTAWFSEFTPETIVTRDEQRIRAFHQAKGDIILKPLDGMGGSSIFRVKKDDPNL
HHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCC
GVIIETLTAEGSQYAMAQAFIPDITAGDKRILVVDGEPVPYSLARIPQKGETRGNLAAGG
EEEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCCC
RGVAQPLSESDWHIARTIGPELKKRGLIFVGLDVIGDKLTEINVTSPTCIKEIEAAFDVD
CCCCCCCCCCCCEEEECCCHHHHHCCEEEEEHHHHCCCEEEEECCCCHHHHHHHHHHCCC
ITGMLMDAIEQRINKSA
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]